Alternations in the gut microbiota and metabolome with newly diagnosed unstable angina

Abstract Background: Type 2 diabetes (T2DM) is a top risk factor for health in China. Gut microbiota, genetic factors and lipids metabolism play important role in development of T2DM. In this study, we investigated the relationship between the gut microbiota and omentin-1 gene polymorphism to explore the interaction between host gene and gut microbiota in Uyghur T2DM. Methods: A total of 98 newly diagnosed Uyghur T2DM patients and 99 healthy normal controls (NC) enrolled into this study according to inclusion criteria. The total DNAs was extracted from the fecal microbiota. Abundance of the Lactobacillus genus, Bacteroides thetaiotaomicron and Clostridium in the gut microbiota was determined with 16S rDNA gene Real-time fluorescence quantitative PCR amplification. PCR-PFLP was applied to determine the genotypes of Val109Asp variant (rs2274907) in the Omentin-1 gene. And the relationship between rs2274907 and gut microbiota was assessed. Results: There were no significant differences of the Val109Asp variant (rs2274907) between T2DM and NC group. The abundance of Lactobacillus genus and Clostridium genus was lower in newly diagnosed T2DM group than in the NC group (P<0.05). Serum insulin, LDL-C, the abundances of Lactobacillus genus and Clostridium genus were the risk factors of T2DM. (OR=1.094 95%CI 1.014-1.180), (OR=3.868 95%CI 1.250-11.971), (OR=0.288 95%CI 0.145-0.571), (OR=0.044 95%CI 0.012-0.154). Conclusions: The abundance of Lactobacillus and Clostridium genus may be related to the pathogenesis of new-onset T2DM in Uyghur population, the mechanism of which needs to be further studied. The interactive relationship between the gut microbiota and omentin-1 gene polymorphism in newly diagnosed T2DM was not observed in this study.

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Altered Profile of Fecal Microbiota in Newly Diagnosed Systemic Lupus Erythematosus Egyptian Patients

International Journal of Microbiology ◽

10.1155/2021/9934533 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Marian A. Gerges ◽

Noura E. Esmaeel ◽

Wafaa K. Makram ◽

Doaa M. Sharaf ◽

Manar G. Gebriel

Keyword(s):

Systemic Lupus Erythematosus ◽

Gut Microbiota ◽

Disease Activity ◽

Lupus Erythematosus ◽

Healthy Subjects ◽

Fecal Microbiota ◽

Healthy Controls ◽

Newly Diagnosed ◽

Systemic Lupus ◽

Egyptian Patients

Background. Dysbiosis of gut microbiota could promote autoimmune disorders including systemic lupus erythematosus (SLE). Clarifying this point would be of great importance in understanding the pathogenesis and hence the development of new strategies for SLE treatment. Aim. This study aimed to determine the fecal microbiota profile in newly diagnosed SLE patients compared to healthy subjects and to investigate the correlation of this profile with disease activity. Methods. Newly diagnosed SLE patients who fulfilled at least four of the American College of Rheumatology (ACR) criteria were enrolled during the study period. Patients with lupus were matched to healthy subjects. SLE activity was evaluated using the Systemic Lupus Disease Activity Index (SLEDAI-2K). Fresh fecal samples were collected from each subject. Genomic DNA was extracted from fecal samples. Quantitative real-time PCR was applied for quantitation of Firmicutes phylum, Bacteroidetes phylum, and Lactobacillus genus in comparison to the total fecal microbiota. Results of patients’ samples were compared to those of healthy subjects and were correlated to patients’ SLEDAI-2K score. Results. Twenty SLE patients’ samples were compared with 20 control samples. There was a significant alteration in SLE patients’ gut microbiota. A significantly lower ( p ≤ 0.001 ) Firmicutes/Bacteroidetes (F/B) ratio in SLE patients (mean ratio: 0.66%) compared to healthy subjects (mean ratio: 1.79%) was found. Lactobacillus showed a significant decrease in SLE patients ( p = 0.006 ) in comparison to healthy controls. An inverse significant correlation between SLEDAI-2K scores for disease activity and F/B ratio (r = −0.451; p = 0.04 ) was found. However, an inverse nonsignificant correlation between SLEDAI-2K scores for disease activity and Lactobacillus (r = −0.155; p = 0.51 ) was detected. Conclusion. Compared to healthy controls, recently diagnosed SLE Egyptian patients have an altered fecal microbiota profile with significant lowering of both F/B ratio and Lactobacillus abundance, which is weakly correlated with disease activity.

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Effect of Jianpi Qinghua Decoction on Gut Microbiota, Intestinal Permeability and Glycolipid Metabolism in Newly Diagnosed T2DM Patients: Study Protocol for a Randomized, Double-blind, Placebo-controlled Clinical Trial

10.21203/rs.3.rs-633298/v1 ◽

2021 ◽

Author(s):

Shenyi Jin ◽

Sheng Zhang ◽

Qingguang Chen ◽

Xu Han ◽

Yahua Liu ◽

...

Keyword(s):

Clinical Trial ◽

Gut Microbiota ◽

Intestinal Permeability ◽

Density Lipoprotein ◽

Lipoprotein Cholesterol ◽

Postprandial Blood Glucose ◽

Controlled Clinical Trial ◽

Newly Diagnosed ◽

Double Blind ◽

Double Blind Placebo

Abstract BackgroundThe gut microbiome is a key target for traditional Chinese medicines (TCM) to ameliorate type 2 diabetes mellitus (T2DM). Patients with T2DM have quite distinct gut microbiota in comparisons with normal individuals, and it directly influences intestinal permeability. This study aims to investigate the underlying effects of Jianpi Qinghua Decoction on gut microbiota and intestinal permeability in newly diagnosed T2DM patients.Methods / designThis study is a single-center, randomized, double-blind, placebo-controlled clinical trial in 120 subjects with newly diagnosed T2DM. The patients will be randomized in a 1:1 ratio into placebo group (1/10 Jianpi Qinghua Decoction + maltodextrin twice daily, n = 60) and treatment group (Jianpi Qinghua Decoction twice daily, n = 60). The study drugs will be double blinded to both investigators and participants. The visits will be done at baseline (visit 0), 1 month (visit 1), 2 months (visit 2), 3 months (visit 3). Measures include: anthropometric measures, blood pressure, glycosylated hemoglobin (HbA1c), fasting plasma glucose (FPG), 2-hour postprandial blood glucose (2hPG) and serum insulin, total cholesterol (TC), total triglycerides (TG), low-density lipoprotein cholesterol (LDL) and high-density lipoprotein cholesterol (HDL), safety indexs (including blood routine examination, clinical urine tests, liver/renal profile), identifying intestinal permeability used ELISA testing serum LPS, serum intestinal fatty acid binding protein (IFABP), serum Zonulin concentrations and fecal sample collection for gut microbiota profiling through 16S rRNA gene sequencing. Data will be analyzed using SPSS version 26. DiscussionWe describe a clinical research protocol evaluating the impact of Jianpi Qinghua Decoction on gut microbiota and intestinal permeability in newly diagnosed T2DM patients. We assume that adminstration of Jianpi Qinghua Decoction will correct gut microbial dysbiosis and intestinal permeability, leading to improved glycemic control.Trial registrationChinese clinical trial registry, identifier: ChiCTR2000039423（http://www.chictr.org.cn/edit.aspx?pid=62537&htm=4.）.Registered on 27 October 2020.

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Gut microbiota composition in patients with newly diagnosed bipolar disorder and their unaffected first-degree relatives

Brain Behavior and Immunity ◽

10.1016/j.bbi.2018.09.026 ◽

2019 ◽

Vol 75 ◽

pp. 112-118 ◽

Cited By ~ 42

Author(s):

Klara Coello ◽

Tue Haldor Hansen ◽

Nikolaj Sørensen ◽

Klaus Munkholm ◽

Lars Vedel Kessing ◽

...

Keyword(s):

Bipolar Disorder ◽

Gut Microbiota ◽

Microbiota Composition ◽

Newly Diagnosed ◽

First Degree Relatives ◽

Gut Microbiota Composition

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Altered Gut Microbiota Is Present in Newly Diagnosed Pediatric Patients With Inflammatory Bowel Disease

Journal of Pediatric Gastroenterology and Nutrition ◽

10.1097/mpg.0000000000002611 ◽

2020 ◽

Vol 70 (4) ◽

pp. 497-502 ◽

Cited By ~ 2

Author(s):

Sara Sila ◽

Marko Jelić ◽

Ivana Trivić ◽

Arjana Tambić Andrašević ◽

Iva Hojsak ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Gut Microbiota ◽

Bowel Disease ◽

Pediatric Patients ◽

Newly Diagnosed ◽

Inflammatory Bowel

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Plasma asymmetric dimethylarginine concentrations in newly diagnosed patients with acute myocardial infarction or unstable angina pectoris during two weeks of medical treatment

The American Journal of Cardiology ◽

10.1016/j.amjcard.2004.11.023 ◽

2005 ◽

Vol 95 (6) ◽

pp. 729-733 ◽

Cited By ~ 50

Author(s):

Sung Won Bae ◽

Markus C. Stühlinger ◽

Hwan Soo Yoo ◽

Kyung Hyun Yu ◽

Haing Kee Park ◽

...

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Angina Pectoris ◽

Medical Treatment ◽

Unstable Angina ◽

Asymmetric Dimethylarginine ◽

Unstable Angina Pectoris ◽

Newly Diagnosed

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Effects ofLactobacillus rhamnosusGG andBifidobacterium lactisBb12 on beta-cell function in children with newly diagnosed type 1 diabetes: protocol of a randomised controlled trial

BMJ Open ◽

10.1136/bmjopen-2017-017178 ◽

2017 ◽

Vol 7 (10) ◽

pp. e017178 ◽

Cited By ~ 15

Author(s):

Lidia Groele ◽

Hania Szajewska ◽

Agnieszka Szypowska

Keyword(s):

Type 1 Diabetes ◽

Gut Microbiota ◽

Beta Cell ◽

Beta Cell Function ◽

Cell Function ◽

Controlled Trial ◽

Lactobacillus Rhamnosus ◽

Newly Diagnosed ◽

Double Blind

IntroductionRecent evidence has demonstrated that, among other factors, dysbiosis (imbalances in the composition and function of the gut microbiota) may be relevant in the development of type 1 diabetes (T1D). Thus, gut microbiota may be a target for improving outcomes in subjects with T1D. The aim of the study is to examine the effects ofLactobacillus rhamnosusGG andBifidobacterium lactisBb12 on beta-cell function in children with newly diagnosed T1D.Methods and analysisA total of 96 children aged 8 to 17 years with newly diagnosed T1D, confirmed by clinical history and the presence of at least one positive autoantibody, will be enrolled in a double-blind, randomised, placebo-controlled trial in which they will receiveL. rhamnosusGG andB. lactisBb12 at a dose of 109colony-forming units or an identically appearing placebo, orally, once daily, for 6 months. The follow-up will be for 12 months. The primary outcome measures will be the area under the curve of the C-peptide level during 2-hour responses to a mixed meal.Ethics and disseminationThe Bioethics Committee approved the study protocol. The findings of this trial will be submitted to a peer-reviewed paediatric journal. Abstracts will be submitted to relevant national and international conferences.Trial registration numberNCT03032354; Pre-results.

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Associations Between Gut Microbiota and Asthma Endotypes: A Cross-Sectional Study in South China Based on Patients with Newly Diagnosed Asthma

Journal of Asthma and Allergy ◽

10.2147/jaa.s320088 ◽

2021 ◽

Vol Volume 14 ◽

pp. 981-992

Author(s):

Xiao-Ling Zou ◽

Jin-Jie Wu ◽

Hui-Xia Ye ◽

Ding-Yun Feng ◽

Ping Meng ◽

...

Keyword(s):

Gut Microbiota ◽

South China ◽

Cross Sectional Study ◽

Newly Diagnosed ◽

Sectional Study ◽

Cross Sectional

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Correlation study on gut microbiota and omentin-1 gene polymorphism in Uyghur newly diagnosed type 2 diabetes

10.21203/rs.2.22391/v1 ◽

2020 ◽

Author(s):

Rebiya Nuli ◽

Jiaoyu Shan ◽

Bing Zhang ◽

Rui Li ◽

Yaqun Guan

Keyword(s):

Type 2 Diabetes ◽

Risk Factor ◽

Gene Polymorphism ◽

Gut Microbiota ◽

Serum Insulin ◽

Correlation Study ◽

Control Group ◽

Newly Diagnosed ◽

The Relationship

Abstract Background: Type 2 diabetes (T2DM) is a top risk factor for health in China. Gut microbiota, genetic factors and lipids metabolism play important role in development of T2DM. In this study, we investigated the relationship between the gut microbiota and omentin-1 gene polymorphism to explore host gene and gut microbiota interaction in Uyghur T2DM.Methods: A total of 98 newly diagnosed Uyghur T2DM patients and 99 healthy normal controls (NC) enrolled into this study according to inclusion criteria. The total DNAs were extracted from the fecal microbiota. Abundant of the Lactobacillus genus, Bacteroides thetaiotaomicron and Clostridium in the gut microbiota were determined with 16S rDNA gene Real-time fluorescence quantitative PCR amplification. PCR-PFLP was applied to determine the genotypes of Val109Asp variant (rs2274907) in the Omentin-1 gene. And the relationship between rs2274907 and gut microbiota was assessed.Results : There were no significant differences between the genotypes of Val109Asp variant (rs2274907) of T2DM and control group. The abundances of Lactobacillus genus and Clostridium genus were lower in newly diagnosed T2DM group, compared with NC group ( P <0.05). Serum insulin, LDL-C, the abundances of Lactobacillus genus and Clostridium genus were the risk factor of T2DM. (OR=1.094 95%CI 1.014-1.180), (OR=3.868 95%CI 1.250-11.971), (OR=0.288, 95%CI 0.145-0.571), (OR=0.044, 95%CI 0.012-0.154).Conclusions: The abundance of Lactobacillus and Clostridium genus may be related to the pathogenesis of new-onset T2DM in Uyghur population, the mechanism of which needs to be further studied. The interaction relationship between the gut microbiota and omentin-1 gene polymorphism in newly diagnosed T2DM was not observed in this study.

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