Complexation of epigallocatechin gallate (a green tea extract, egcg) with Mn2+: nuclear spin relaxation by the paramagnetic ion

Duchenne muscular dystrophy is a frequent muscular disorder caused by mutations in the gene encoding dystrophin, a cytoskeletal protein that contributes to the stabilization of muscle fiber membrane during muscle activity. Affected individuals show progressive muscle wasting that generally causes death by age 30. In this study, the dystrophic mdx 5Cv mouse model was used to investigate the effects of green tea extract, its major component (−)-epigallocatechin gallate, and pentoxifylline on dystrophic muscle quality and function. Three-week-old mdx 5Cv mice were fed for either 1 or 5 wk a control chow or a chow containing the test substances. Histological examination showed a delay in necrosis of the extensor digitorum longus muscle in treated mice. Mechanical properties of triceps suræ muscles were recorded while the mice were under deep anesthesia. Phasic and tetanic tensions of treated mice were increased, reaching values close to those of normal mice. The phasic-to-tetanic tension ratio was corrected. Finally, muscles from treated mice exhibited 30–50% more residual force in a fatigue assay. These results demonstrate that diet supplementation of dystrophic mdx 5Cv mice with green tea extract or (−)-epigallocatechin gallate protected muscle against the first massive wave of necrosis and stimulated muscle adaptation toward a stronger and more resistant phenotype.

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Kinetics of Reduction of Ferrylmyoglobin by (−)-Epigallocatechin Gallate and Green Tea Extract

Journal of Agricultural and Food Chemistry ◽

10.1021/jf011535u ◽

2002 ◽

Vol 50 (10) ◽

pp. 2998-3003 ◽

Cited By ~ 27

Author(s):

Min Hu ◽

Leif H. Skibsted

Keyword(s):

Green Tea ◽

Epigallocatechin Gallate ◽

Green Tea Extract ◽

Tea Extract ◽

Kinetics Of ◽

Kinetics Of Reduction

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Bioavailability and antioxidant effect of epigallocatechin gallate administered in purified form versus as green tea extract in healthy individuals

The Journal of Nutritional Biochemistry ◽

10.1016/j.jnutbio.2005.03.003 ◽

2005 ◽

Vol 16 (10) ◽

pp. 610-616 ◽

Cited By ~ 110

Author(s):

Susanne M. Henning ◽

Yantao Niu ◽

Yong Liu ◽

Nicolas H. Lee ◽

Yukihiko Hara ◽

...

Keyword(s):

Green Tea ◽

Epigallocatechin Gallate ◽

Antioxidant Effect ◽

Green Tea Extract ◽

Healthy Individuals ◽

Form Versus ◽

Tea Extract

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The MIRACLE trial: A randomized, controlled trial comparing green tea extract versus placebo for the prevention of metachronous colon adenomas in a screening population.

Journal of Clinical Oncology ◽

10.1200/jco.2015.33.3_suppl.tps786 ◽

2015 ◽

Vol 33 (3_suppl) ◽

pp. TPS786-TPS786 ◽

Cited By ~ 1

Author(s):

Thomas Jens Ettrich ◽

Julia Stingl ◽

Rainer Muche ◽

Katrin Claus ◽

Baerbel Reiser ◽

...

Keyword(s):

Green Tea ◽

Controlled Trial ◽

Epigallocatechin Gallate ◽

Genetic Alterations ◽

Colorectal Polyps ◽

Green Tea Extract ◽

Colorectal Adenomas ◽

Tissue Samples ◽

Increased Risk ◽

Tea Extract

TPS786 Background: Prevention of colorectal cancer is a major health care issue. After polypectomy there is an increased risk of polyp recurrence and various means of chemoprevention have been tried to prevent this. NSAIDs have been shown to be effective but confer side effects such as gastrointestinal bleeding. Nutraceuticals such as polyphenols from tea plants have demonstrated remarkable therapeutic and preventive effects in molecular, epidemiological and some clinical trials. However, their value in preventing colorectal polyps has not been demonstrated in a large, randomized trial. The beneficial safety profile of decaffeinated green tea extract and accumulating evidence of its cancer preventive potential justify and require, in our view, a validation of this compound for the nutriprevention of colorectal adenoma. Good accessibility and low costs might render this neutraceutical a top candidate for wider use as nutritional supplement in colon cancer prevention. Methods: Randomized, double blinded, placebo-controlled, multicenter trial. After a one month run-in period with verum, 918 patients (age: 50-80 years) who have undergone polypectomy within the last 6 months will be randomized to receive either decaffeinated green tea extract (containing 150 mg epigallocatechin gallate (EGCG) two times daily) or placebo over a period of three years. Primary outcome: Incidence of metachronous colorectal adenomas (tubulovillous, tubular, villous, serrated lesions) at the 3 year follow-up colonoscopy. Secondary outcomes: Occurrences, number, localization, size and histological subtypes of adenomas, frequency of colorectal carcinoma. In addition, genetic and biochemical biomarkers in blood samples and genetic alterations (Ras, B-raf, microRNAs) in tissue samples of adenomas will be analyzed (biobanking subprojects). Additionally, nutrikinetics and nutrigenetics of EGCG and other catechins will be assessed in healthy volunteers. Patient recruitment has started in November 2011. At September 2014, 785 patients were recruited and 651 patients were randomized. We expect the last patient out in Spring 2018. (Trial identifier NCT01360320) Clinical trial information: NCT01360320.

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Green tea extract improves endurance capacity and increases muscle lipid oxidation in mice

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00693.2004 ◽

2005 ◽

Vol 288 (3) ◽

pp. R708-R715 ◽

Cited By ~ 131

Author(s):

Takatoshi Murase ◽

Satoshi Haramizu ◽

Akira Shimotoyodome ◽

Azumi Nagasawa ◽

Ichiro Tokimitsu

Keyword(s):

Fatty Acid ◽

Green Tea ◽

Epigallocatechin Gallate ◽

Fat Oxidation ◽

Green Tea Extract ◽

Endurance Capacity ◽

Oxidation Activity ◽

Tea Extract ◽

High Level ◽

Dose Dependent

Green tea contains a high level of polyphenolic compounds known as catechins. We investigated the effects of green tea extract (GTE), which is rich in catechins, on endurance capacity, energy metabolism, and fat oxidation in BALB/c mice over a 10-wk period. Swimming times to exhaustion for mice fed 0.2–0.5% (wt/wt) GTE were prolonged by 8–24%. The effects were dose dependent and accompanied by lower respiratory quotients and higher rates of fat oxidation as determined by indirect calorimetry. In addition, feeding with GTE increased the level of β-oxidation activity in skeletal muscle. Plasma lactate concentrations in mice fed GTE were significantly decreased after exercise, concomitant with increases in free fatty acid concentrations in plasma, suggesting an increased lipid use as an energy source in GTE-fed mice. Epigallocatechin gallate (EGCG), a major component of tea catechins, also enhanced endurance capacity, suggesting that the endurance-improving effects of GTE were mediated, at least in part, by EGCG. The β-oxidation activity and the level of fatty acid translocase/CD36 mRNA in the muscle was higher in GTE-fed mice compared with control mice. These results indicate that GTE are beneficial for improving endurance capacity and support the hypothesis that the stimulation of fatty acid use is a promising strategy for improving endurance capacity.

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Limited Sampling Estimates of Epigallocatechin Gallate Exposures in Cirrhotic and Noncirrhotic Patients With Hepatitis C After Single Oral Doses of Green Tea Extract

Clinical Therapeutics ◽

10.1016/j.clinthera.2012.10.009 ◽

2012 ◽

Vol 34 (12) ◽

pp. 2279-2285.e1 ◽

Cited By ~ 12

Author(s):

Dina Halegoua-De Marzio ◽

Walter K. Kraft ◽

Constantine Daskalakis ◽

Xie Ying ◽

Roy L. Hawke ◽

...

Keyword(s):

Hepatitis C ◽

Green Tea ◽

Epigallocatechin Gallate ◽

Green Tea Extract ◽

Limited Sampling ◽

Tea Extract ◽

Oral Doses

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Anti-inflammatory Activity of Camellia sinensis, l. Extract Cream Combined with Vitamin C as Antioxidant on Croton Oil-induced Inflamation in Male Mice Strain BALB/C

Majalah Obat Tradisional ◽

10.22146/tradmedj.27915 ◽

2017 ◽

Vol 22 (2) ◽

pp. 73

Author(s):

Nining Sugihartini ◽

Ratih Saridewi ◽

Ulfa Ramdhani M ◽

Fitri Rahmawanti ◽

Sapto Yuliani ◽

...

Keyword(s):

Vitamin C ◽

Green Tea ◽

Epigallocatechin Gallate ◽

Inflammatory Cells ◽

Green Tea Extract ◽

Inflammatory Activity ◽

Inflammatory Parameters ◽

Cox 2 ◽

Anti Inflammatory ◽

Tea Extract

Green tea extract cream contains epigallocatechin gallate (EGCG) as the active ingredient for anti-inflammatory. Epigallocatechin gallate is easyly oxidized and able to reduce its effectivity as an anti-inflammatory. Therefore, an addition of antioxidants in order to increase its stability is required. The purpose of this study was to determine the effect of adding the antioxidant Vitamin C on the effectivity of green tea extract as an anti-inflammatory. This study uses 6 groups of male mice strain BALB/C which were given treatment as follows: normal control, negative control, base cream, green tea extract (0.2%), Vitamin C cream (1%) and green tea extract cream with addition of Vitamin C. The anti-inflammatory activity was evaluated based on the expression of COX-2, inflammatory cells and the thickness of the epidermis in the skin tissue of mice after given crotton oil (0.1%) on the back for the induction of inflammation. After treatment cream for 3 days, mice were sacrificed for histopathological tissue preparations made with hematoxylin eosin staining and immunohistochemistry COX-2. Data were analyzed statistically with one way Anova followed by t-test to determine differences between groups at a significance level of 0.05. The test results indicate that cream of green tea extract is higher in decreasing inflammatory parameters in comparison with cream of Vitamin C, except in the thickness of epidermal parameter. Green tea extract cream with the addition of Vitamin C is higher in reducing inflammatory parameters than cream of green tea extract or cream of Vitamin C. The decline percentage of cells that express COX-2, inflammatory cells and the thickness of the epidermis in the each of groups were cream of green tea extract:57.95%;53.75%;34.83%, cream of Vitamin C:48.76%;34.96%;34.27%, cream of green tea extract and Vitamin C:61,89%;65,54%;46.30%, respectively. Based on the results of this study, it can be concluded that anti-inflammatory activity of green tea extract cream increased due to the addition of 1% vitamin C as an antioxidant.

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