Human C-reactive protein aggravates osteoarthritis development in mice on a high-fat diet

The aim of this study was to investigate the real impact of dietary lipids on metabolic and inflammatory response in rat white adipose tissue. Male healthy Wistar rats were fed ad libitum with a control diet (CON, n=12) or with an adjusted high-fat diet (HFD, n=12) for 12 weeks. Oral glucose and insulin tolerance tests were performed during the last week of the protocol. Plasma fatty acid, lipid profile, body adiposity, and carcass chemical composition were analyzed. Plasma concentration of leptin, adiponectin, C-reactive protein (CRP), TNF-α, IL-6, and monocyte chemotactic protein (MCP-1) was measured. Periepididymal adipose tissue was employed to evaluate TNF-α, MCP-1, and adiponectin gene expression as well as NF-κB pathway and AKT proteins. Isocaloric intake of the adjusted HFD did not induce hyperphagia, but promoted an increase in periepididymal (HFD = 2.94 ± 0.77 vs. CON = 1.99 ± 0.26 g/100 g body weight, p = 0.01) and retroperitoneal adiposity (HFD = 3.11 ± 0.81 vs. CON = 2.08 ± 0.39 g/100 g body weight, p = 0.01) and total body lipid content (HFD = 105.3 ± 20.8 vs. CON = 80.5 ± 7.6 g carcass, p = 0.03). Compared with control rats, HFD rats developed glucose intolerance (p=0.01), dyslipidemia (p = 0.02) and exhibited higher C-reactive protein levels in response to the HFD (HFD = 1002 ± 168 vs. CON = 611 ± 260 ng/mL, p = 0.01). The adjusted HFD did not affect adipokine gene expression or proteins involved in inflammatory signaling, but decreased AKT phosphorylation after insulin stimulation in periepididymal adipose tissue (p = 0.01). In this study, nutrient-adjusted HFD did not induce periepididymal adipose tissue inflammation in rats, suggesting that the composition of HFD differently modulates inflammation in rats, and adequate micronutrient levels may also influence inflammatory pathways.

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Low Carbohydrate, High Fat Diet Increases C-Reactive Protein during Weight Loss

Journal of the American College of Nutrition ◽

10.1080/07315724.2007.10719598 ◽

2007 ◽

Vol 26 (2) ◽

pp. 163-169 ◽

Cited By ~ 39

Author(s):

Janet W. Rankin ◽

Abigail D. Turpyn

Keyword(s):

Weight Loss ◽

High Fat Diet ◽

C Reactive Protein ◽

High Fat ◽

Reactive Protein ◽

Low Carbohydrate

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Vitamin D reduces high-fat diet induced weight gain and C-reactive protein, increases interleukin-10, and reduces CD86 and caspase-3

Pathophysiology ◽

10.1016/j.pathophys.2017.01.003 ◽

2017 ◽

Vol 24 (1) ◽

pp. 31-37 ◽

Cited By ~ 5

Author(s):

Asmaa M.S. Gomaa ◽

Ebtihal A. Abd El-Aziz

Keyword(s):

Vitamin D ◽

Weight Gain ◽

High Fat Diet ◽

Caspase 3 ◽

Interleukin 10 ◽

C Reactive Protein ◽

High Fat ◽

Reactive Protein

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Aggravated osteoarthritis development in mice transgenic for human C-reactive protein on a high fat diet

Osteoarthritis and Cartilage ◽

10.1016/j.joca.2016.01.670 ◽

2016 ◽

Vol 24 ◽

pp. S375

Author(s):

A. Kozijn ◽

F. van den Ham ◽

F. Casbas ◽

C. Hodgman ◽

J. Bacardit ◽

...

Keyword(s):

High Fat Diet ◽

C Reactive Protein ◽

High Fat ◽

Reactive Protein

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A Possible Role for Chemerin; a Novel Adipokine in the Haemostatic and Atherogenic Disorders Related to Obesity in White Albino Rats

The Open Obesity Journal ◽

10.2174/1876823720130703010 ◽

2013 ◽

Vol 5 (1) ◽

pp. 65-71 ◽

Cited By ~ 2

Author(s):

Enas N. Morgan ◽

Husam M. Edrees

Keyword(s):

High Fat Diet ◽

Bleeding Time ◽

Serum Levels ◽

Significant Negative Correlation ◽

Albino Rats ◽

C Reactive Protein ◽

High Fat ◽

Reactive Protein ◽

Group I ◽

Insignificant Correlation

A strong link between obesity, hypercoagulability and thrombogenesis, had been recognized. Chemerin is a novel adipokine that has been activated by the coagulation process and suggested to play an important role in the pathogenesis of metabolic syndrome. We aimed to identify the possible relationship between chemerin levels and haemostatic changes in the high fat diet (HFD) fed rats. A total number of 20 adult male albino rats were divided into two groups of 10 rats each. Group I, rats served as controls; Group II: rats received high fat diet (58% fat) for 8 weeks. In both groups, serum levels of glucose, insulin, chemerin, total cholesterol (TC), triglycerides (TG), HDL, LDL were measured. As well as the bleeding time (BT), whole blood clotting time (WBCT), Prothrombin time (PT), Activated partial thromboplastin time (aPTT), fibrinogen level and C- reactive protein (CRP) were measured. Moreover HOMA-IR was calculated for both groups. The results of the current study revealed that chemerine level increased significantly in the HFD- fed rats (p< 0.001). In addition, a significant positive correlation was detected for the serum chemerin levels with body weight, insulin levels, HOMA-IR, the levels of TC, TG, LDL and CRP, while a significant negative correlation was found between its levels and serum levels of HDL. Moreover chemerin was correlated negatively with the BT, WBCT, PT, aPTT and positively correlated with plasma fibrinogen, while there was insignificant correlation with platelet count. These results suggested that chemerin may represent a novel link between obesity and its haemostatic and atherogenic complication.

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Consumption of a high-fat meal containing cheese compared with a vegan alternative lowers postprandial C-reactive protein in overweight and obese individuals with metabolic abnormalities: a randomised controlled cross-over study

Journal of Nutritional Science ◽

10.1017/jns.2015.40 ◽

2016 ◽

Vol 5 ◽

Cited By ~ 9

Author(s):

Elieke Demmer ◽

Marta D. Van Loan ◽

Nancy Rivera ◽

Tara S. Rogers ◽

Erik R. Gertz ◽

...

Keyword(s):

Test Meal ◽

Inflammatory Markers ◽

Cellular Adhesion ◽

Metabolic Abnormalities ◽

C Reactive Protein ◽

High Fat ◽

Reactive Protein ◽

Amyloid A ◽

Alternative Test ◽

Randomised Controlled

AbstractDietary recommendations suggest decreased consumption of SFA to minimise CVD risk; however, not all foods rich in SFA are equivalent. To evaluate the effects of SFA in a dairy food matrix, as Cheddar cheese, v. SFA from a vegan-alternative test meal on postprandial inflammatory markers, a randomised controlled cross-over trial was conducted in twenty overweight or obese adults with metabolic abnormalities. Individuals consumed two isoenergetic high-fat mixed meals separated by a 1- to 2-week washout period. Serum was collected at baseline, and at 1, 3 and 6 h postprandially and analysed for inflammatory markers (IL-6, IL-8, IL-10, IL-17, IL-18, TNFα, monocyte chemotactic protein-1 (MCP-1)), acute-phase proteins C-reactive protein (CRP) and serum amyloid-A (SAA), cellular adhesion molecules and blood lipids, glucose and insulin. Following both high-fat test meals, postprandial TAG concentrations rose steadily (P < 0·05) without a decrease by 6 h. The incremental AUC (iAUC) for CRP was significantly lower (P < 0·05) in response to the cheese compared with the vegan-alternative test meal. A treatment effect was not observed for any other inflammatory markers; however, for both test meals, multiple markers significantly changed from baseline over the 6 h postprandial period (IL-6, IL-8, IL-18, TNFα, MCP-1, SAA). Saturated fat in the form of a cheese matrix reduced the iAUC for CRP compared with a vegan-alternative test meal during the postprandial 6 h period. The study is registered at clinicaltrials.gov under NCT01803633.

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A substitution model of dietary manipulation is an effective means of optimising lipid profile, reducing C-reactive protein and increasing insulin-like growth factor-1

British Journal Of Nutrition ◽

10.1079/bjn20041262 ◽

2004 ◽

Vol 92 (5) ◽

pp. 809-818 ◽

Cited By ~ 9

Author(s):

Adrian H. Heald ◽

Cheryl Golding ◽

Reena Sharma ◽

Kirk Siddals ◽

Sara Kirk ◽

...

Keyword(s):

Growth Factor ◽

Dietary Intervention ◽

Short Interval ◽

The Other ◽

Fat Intake ◽

Insulin Like Growth Factor ◽

C Reactive Protein ◽

High Fat ◽

Substitution Model ◽

Reactive Protein

There are two key methods in which fat intake may be manipulated; the ‘substitution model’ and the ‘reduction model’. However insufficient information is known about the mechanisms of dietary fat reduction in individuals who have successfully reduced their fat intake, to be clear as to which strategy offers the greatest chance of success. Our objective was to ascertain the most effective dietary intervention for improving cardiovascular risk profile. Eighty female volunteers (high fat consumers) were recruited. Each subject was randomly allocated into one of the following groups. Substitution of high-fat foods was made with reduced-fat products, by the reduction of high-fat foods, by a combination of substitution and reduction strategies, or no advice was given. Each intervention lasted 3 months. Anthropometric measures and fasting blood samples were taken at baseline and follow-up. The substitution intervention resulted in weight loss (mean −1.4 (95% CI −2.4, −0.2) kg) and reduced percentage body fat (mean −1.3 (95% CI −2.0, −0.5)%). There was no significant weight change with the other interventions. Fasting triacylglycerols (−0.2 (SEM 0.07) mM; P=0.04), cholesterol and C-reactive protein (CRP) levels (0.8 (SEM 0.2) mg/l; P=0.04) fell with the substitution intervention, but not with the other interventions. Insulin-like growth factor-1 increased with both substitution and reduction (P=0.02). There was no significant change in fasting insulin or glucose with any intervention. The substitution model of dietary intervention is effective even over a relatively short interval of time in reducing fasting total cholesterol, triacylglycerols and CRP. Although the group size for the present study was small and involved females only, it has significant implications for population intervention strategies.

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