Tertiary Dentin Formation after Direct Pulp Capping with Odontogenic Ameloblast-associated Protein in Rat Teeth

2010 ◽  
Vol 36 (12) ◽  
pp. 1956-1962 ◽  
Author(s):  
In-Seok Yang ◽  
Dong-Seol Lee ◽  
Jong-Tae Park ◽  
Heung-Joong Kim ◽  
Ho-Hyun Son ◽  
...  
Keyword(s):  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Tertiary Dentin ◽  
Dentin Formation

Direct pulp capping in permanent teeth in children – tertiary dentin formation, materials used. Part II

2018 ◽  
Vol 23 (2) ◽  
Author(s):  
Malwina Kolasa ◽  
Joanna Szczepańska
Keyword(s):  
Permanent Teeth ◽  
Clinical State ◽  
Direct Pulp Capping ◽  
Therapeutic Success ◽  
Pulp Capping ◽  
Dental Tissues ◽  
Tertiary Dentin ◽  
Continue Research ◽  
Materials Used ◽  
Dentin Formation

Direct pulp capping involves placing therapeutic material on mechanically or traumatically exposed pulp. The most essential requirement of therapeutic success is clinical state of the pulp which should be healthy or in reversible pulpitis. The method is particularly recommended for young permanent teeth due to the high regenerative potential of dental pulp. The mechanisms underlying these repair processes involve the ability of odontoblasts to form dentin bridges via tertiary dentin deposition. If pulp exposure occurs, a layer of odontoblasts is killed and must be replaced with a new odontoblastic population, which differentiates from pulpal stem cells under the influence of a therapeutic agent applied on the pulp. In addition to odontotropic properties and the ability to stimulate production of qualitatively satisfying dentinal bridge, the substance used for pulp capping should be biocompatible, not stain dental tissues, exhibit good adhesion to them, and insolubility in dentin tubule fluid or water. So far an agent which meets all the requirements mentioned above has not been invented. It is crucial to continue research to develop a substance that will yield the best effects in direct pulp capping.

scholarly journals Induction of reparative dentin by calcium silicate-based material as direct pulp capping agent

2019 ◽  
Vol 12 (4) ◽  
pp. 182-186
Author(s):  
Mozammal Hossain ◽  
Mahmood Sajedeen ◽  
Yukio Nakamura
Keyword(s):  
Calcium Hydroxide ◽  
Calcium Silicate ◽  
Normal Function ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Exposure Site ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

This study was performed to examine whether calcium silicate could induce reparative dentin formation without eliciting any adverse effect in direct pulp capping of premolar teeth. Twenty participants who need extraction of their 4 healthy permanent premolar teeth for orthodontic reasons were included in this study. Following the surgical procedure, the exposed pulp tissue was treated either with calcium silicate or covered with calcium hydroxide paste. On day 3, 7, 14 and 28, the experimental teeth was extracted and examined using light microscopy and histometric analysis to observe the inflammatory changes and the amount of reparative dentin formation. The results showed that in the calcium silicate treated teeth, substantial amounts of dentine-like tissue was formed on day 14 and mostly located on the exposure site. It was also observed in the calcium hydroxide treated teeth but dentin-like tissue located at a distance from the exposure site. The total amount of reparative dentine formed in the calcium silicate-treated teeth was significantly higher (p<0.005) than in the calcium hydroxide-treated specimens. In conclusion that the calcium silicate indices pulpal wound healing and reparative formation in the exposed teeth without affecting the normal function of the remaining pulp.

Tertiary Dentin Formation after Indirect Pulp Capping Using Protein CPNE7

2016 ◽  
Vol 95 (8) ◽  
pp. 906-912 ◽  
Author(s):  
H.W. Choung ◽  
D.S. Lee ◽  
Ji-Hyun Lee ◽  
W.J. Shon ◽  
Jong-Ho Lee ◽  
...  
Keyword(s):  
Pulp Capping ◽  
Tertiary Dentin ◽  
Dentin Formation ◽  
Indirect Pulp Capping

scholarly journals Knowledge, attitude and practise of usage of MTA and bio dentine as direct pulp capping agents - A questionnaire study

2020 ◽  
Vol 11 (SPL3) ◽  
pp. 744-756
Author(s):  
Sudarsan R ◽  
Balaji Ganesh S ◽  
Anjaneyulu K
Keyword(s):  
Calcium Hydroxide ◽  
Undergraduate Students ◽  
Direct Pulp Capping ◽  
Online Questionnaire ◽  
Capping Agents ◽  
Pulp Capping ◽  
Reparative Dentin ◽  
Study Population ◽  
Spss Software ◽  
Dentin Formation

Direct pulp-capping is a procedure in which exposed vital pulp is treated by placing a dental material over dental pulp to maintain vitality and to facilitate reparative dentin formation. The most commonly used pulp-capping material for decades is calcium hydroxide. But due to a number of disadvantages, there is a need for an alternate ideal pulp capping material. Recently MTA and Biodentine have been suggested as promising candidates for use in direct pulp capping procedures. The aim of the present study is to evaluate the knowledge, attitude and practise of usage of MTA and biodentine as direct pulp capping agents. An online questionnaire consisting of 15 questions which included demographic details, and questions regarding their knowledge and awareness of direct pulp therapy and the properties of MTA and biodentine. The study was conducted among undergraduate students of a private dental institute. Data was collected from filled questionnaires and analysed using SPSS software. Majority of the respondents (40.1%) chose calcium hydroxide as the material of choice for direct pulp capping and only 30% of the study population chose both MTA and biodentine as a direct pulp capping agent, which was statistically significant (p=0.000). 52% of the respondents were not aware of the major drawbacks of MTA. Knowledge regarding properties of biodentine in comparison to MTA was higher. Within the limits of the study, it shows that the knowledge in regard to the use of MTA and biodentine as direct pulp capping was poor despite recent studies showing a growing number of advantages over calcium hydroxide.

Endodontic Treatment of a Traumatic Tusk Fracture With Exposed Pulp in an Asian Elephant (Elephas maximus)

2021 ◽  
Vol 38 (3) ◽  
pp. 139-151
Author(s):  
Matthias Seewald ◽  
Christine Gohl ◽  
Monika Egerbacher ◽  
Stephan Handschuh ◽  
Kirsti Witter
Keyword(s):  
Photodynamic Therapy ◽  
Treatment Options ◽  
Asian Elephant ◽  
Elephas Maximus ◽  
Filling Material ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Reparative Dentin ◽  
Infection And Inflammation ◽  
Dentin Formation

Tusk fracture in elephants is a common incident often resulting in pulp exposure and pulpitis. Extensive lavage, endodontic therapy, direct pulp capping, or extraction are treatment options. In this report, the successful management of a broken tusk of a juvenile male Asian elephant (Elephas maximus) including morphological analysis of the tusk tip 2 years after surgery are presented. Treatment was carried out under barn conditions and included antimicrobial photodynamic therapy and partial pulpotomy with direct pulp capping. Immediate pain relief was reached. The fractured tusk was preserved and continued to grow. The therapeutic filling material remained intact for over 1 year but was absent 2 years after treatment. The former pulp cavity of the tusk tip was filled with reparative dentin, osteodentin, and bone, but the seal between these hard tissues and pulp chamber dentin was incomplete. Radiographs obtained 3 years after treatment showed no differences in pulp shape, pulp width, and secondary dentin formation between the treated right and the healthy left tusk. It can be concluded that in case of an emergency, the endodontic therapy of a broken elephant tusk can be attempted under improvised conditions with adequate success. Photodynamic therapy might contribute to prevent infection and inflammation of the pulp. The decision tree published by Steenkamp (2019) provides a valuable tool to make quick decisions regarding a suitable therapy of broken tusks.

scholarly journals Evaluating the potential of an amelogenin-derived peptide in tertiary dentin formation

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Xiu Peng ◽  
Sili Han ◽  
Kun Wang ◽  
Longjiang Ding ◽  
Zhenqi Liu ◽  
...  
Keyword(s):  
Cell Viability ◽  
Dental Pulp ◽  
Cell Counting ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Alizarin Red ◽  
Pulp Capping ◽  
Tertiary Dentin ◽  
Dental Pulp Capping ◽  
Dentin Formation

Abstract Several novel biomaterials have been developed for dental pulp capping by inducing tertiary dentin formation. The aim of this study was to evaluate the effect of QP5, an amelogenin-based peptide, on the mineralization of dental pulp cells (DPCs) in vitro and in vivo. The cell viability of human DPCs (hDPCs) after treatment with QP5 was determined using the Cell Counting Kit-8 (CCK-8). Migration of hDPCs was assessed using scratch assays, and the pro-mineralization effect was determined using alkaline phosphatase (ALP) staining, alizarin red staining and the expression of mineralization-related genes and proteins. The results showed that QP5 had little effect on the cell viability, and significantly enhanced the migration capability of hDPCs. QP5 promoted the formation of mineralized nodules, and upregulated the activity of ALP, the expression of mRNA and proteins of mineralization-related genes. A pulp capping model in rats was generated to investigate the biological effect of QP5. The results of micro-computed tomography and haematoxylin and eosin staining indicated that the formation of tertiary dentin in QP5-capping groups was more prominent than that in the negative control group. These results indicated the potential of QP5 as a pulp therapy agent.

scholarly journals Dentin Growth after Direct Pulp Capping with the Different Fractions of Plasma Rich in Growth Factors (PRGF) vs. MTA: Experimental Study in Animal Model

2021 ◽  
Vol 10 (15) ◽  
pp. 3432
Author(s):  
José F. Gaviño-Orduña ◽  
Javier Caviedes-Bucheli ◽  
María C. Manzanares-Céspedes ◽  
Sophie Román-Richon ◽  
Benjamín Martin-Biedma ◽  
...  
Keyword(s):  
Growth Factors ◽  
Mineral Trioxide Aggregate ◽  
Positive Control ◽  
Negative Control ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Vital Pulp Therapy ◽  
Significant Difference ◽  
Group B ◽  
Dentin Formation

Background: This study aimed to evaluate the area of dentin growth in rabbit incisors after pulp capping with plasma rich in growth factors (PRGF) compared with mineral trioxide aggregate (MTA) by fluorescence. Methods: twenty-seven upper and lower incisors of rabbits were divided into 4 groups: poor PRGF (F1) (n = 9 teeth), rich PRGF (F2) (n = 8 teeth), ProRoot MTA (positive control, n = 5 teeth), and untreated (NC) (negative control, n = 5). Fluorochrome markers were injected 24 h before surgery and the day before euthanasia, 28 days after the vital pulp therapy (VPT). Two transverse cuts were performed to every tooth: the first cut (A), 1 mm incisal to the gingival margin, and the second cut (B), 5 mm apical to the first cut. The sections were assessed with histomorphometric evaluation by fluorescence microscopy, comparing the dentin area between fluorescence marks and the total mineralized area. Results: The higher percentage of dentin growth was observed in the F2 group (B = 63.25%, A = 36.52%), followed by F1 (B = 57.63%, A = 30,12%) and MTA (B = 38.64%, A = 15,74%) The group with lowest percentage of dentin growth was the NC group (B = 29.22%, A = 7.82%). Significant difference (p < 0.05) was found between F2 group and MTA, also statistically significant difference has been observed comparing dentin growth areas of NC group with F1 and F2 groups. Conclusions: The application of PRGF rich and poor fraction as a pulp capping material stimulated dentin formation more intensively than MTA and NC.

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