Assessment of physiological age and antioxidant status of new somatic hybrid potato seeds during extended cold storage

From the time of fruit setting early in the spring and throughout the whole summer, samples of fruits were taken directly from the tree. In the fall several bushels of apples were picked and placed in cold storage. These constituted the source of material during the winter. Weekly samples of fruits were analyzed for pH and titratable acidity of their sap, and also for their organic acids content. Various stages in the acid metabolism of fruits were found to correspond chronologically very closely with those of carbohydrate and respiration. During the first two to four weeks of the fruit ontogeny, the pH of the apple juice falls rapidly to a value of about 2.8. Thereafter there is a slow, steady rise which continues until the next spring. This regularity makes the pH of the juice a better indicator of the physiological age of a fruit than either its chemical composition or respiratory rate. Initially, the malic acid content of a fruit is low, and some unknown organic acids predominate. This relation is reversed within the first two to four weeks of growth and during the rest of the ontogeny malic acid forms about 80% of the total organic acids of a fruit.

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Evaluation of chitting to enhance earliness of potatoes grown in southern Ontario

Canadian Journal of Plant Science ◽

10.4141/cjps94-034 ◽

1994 ◽

Vol 74 (1) ◽

pp. 159-165 ◽

Cited By ~ 4

Author(s):

Alan W. McKeown

Keyword(s):

Cold Storage ◽

Potato Cultivars ◽

The Other ◽

Physiological Age ◽

Degree Days ◽

Southern Ontario ◽

Degree Day ◽

Lower Base ◽

Low Degree ◽

Rapid Accumulation

Potato cultivars Atlantic, Conestoga, Jemseg, Superior and Yukon Gold, grown for early markets and mid-season use in Ontario were evaluated for response to chitting in 1987 to 1989. Seed tubers were chitted at 8 °C commencing in January, for an average of 408, 284 or 172 degree-days (base 4 °C). Controls were stored at 4 °C. Plants emerged quicker from chitted seed and produced higher early yields than the controls. Approximately 260 degree-days of chitting was optimal for early yield of Jemseg and Superior. For maximum emergence, 318 degree-days was required for Jemseg. Commencing chitting before March did not increase yields for the other cultivars. Low degree-day requirements are likely the result of cold storage of seed prior to chitting, resulting in more rapid accumulation of degree-days and/or lower base temperatures for growth. Key words:Solarium tuberosum, cultivars, emergence, yield, physiological age

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Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100169080 ◽

1994 ◽

Vol 52 ◽

pp. 270-271

Author(s):

Henry H. Eichelberger ◽

John G. Baust ◽

Robert G. Van Buskirk

Keyword(s):

Cold Storage ◽

Structural Integrity ◽

Culture Media ◽

Cell Structure ◽

Natural State ◽

Sodium Cacodylate ◽

Ruthenium Tetroxide ◽

Cacodylate Buffer ◽

Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

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M.654 Antioxidant status and endothelium dysfunction markers in patients with diabetic retinopathy

Atherosclerosis ◽

10.1016/s0021-9150(04)90652-1 ◽

2004 ◽

Vol 5 (1) ◽

pp. 151

Author(s):

K SIEMIANOWICZ

Keyword(s):

Diabetic Retinopathy ◽

Antioxidant Status ◽

Endothelium Dysfunction

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Association between calcitriol and paricalcitol with oxidative stress in patients with hemodialysis

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000641 ◽

2020 ◽

pp. 1-8

Author(s):

Hasan Haci Yeter ◽

Berfu Korucu ◽

Elif Burcu Bali ◽

Ulver Derici

Keyword(s):

Oxidative Stress ◽

Vitamin D ◽

Antioxidant Status ◽

Total Antioxidant Status ◽

Stress Index ◽

Total Oxidant Status ◽

Vitamin D Analog ◽

Total Antioxidant ◽

Oxidant Status ◽

And Oxidative Stress

Abstract. Background: The pathophysiological basis of chronic kidney disease and its complications, including cardiovascular disease, are associated with chronic inflammation and oxidative stress. We investigated the effects of active vitamin D (calcitriol) and synthetic vitamin D analog (paricalcitol) on oxidative stress in hemodialysis patients. Methods: This cross-sectional study was composed of 83 patients with a minimum hemodialysis vintage of one year. Patients with a history of any infection, malignancy, and chronic inflammatory disease were excluded. Oxidative markers (total oxidant and antioxidant status) and inflammation markers (C-reactive protein and interleukin-6) were analyzed. Results: A total of 47% (39/83) patients were using active or analog vitamin D. Total antioxidant status was significantly higher in patients with using active or analog vitamin D than those who did not use (p = 0.006). Whereas, total oxidant status and oxidative stress index were significantly higher in patients with not using vitamin D when compared with the patients who were using vitamin D preparation (p = 0.005 and p = 0.004, respectively). On the other hand, total antioxidant status, total oxidant status, and oxidative stress index were similar between patients who used active vitamin D or vitamin D analog (p = 0.6; p = 0.4 and p = 0.7, respectively). Conclusion: The use of active or selective vitamin D analog in these patients decreases total oxidant status and increases total antioxidant status. Also, paricalcitol is as effective as calcitriol in decreasing total oxidant status and increasing total antioxidant status in patients with chronic kidney disease.

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Pro-oxidant effects of a high α-tocopherol dose on kidney antioxidant biomarkers and histopathological aspects

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000512 ◽

2017 ◽

Vol 87 (3-4) ◽

pp. 179-190

Author(s):

Amel Kanane ◽

Fayrouz Rouaki ◽

Mohamed Brahim Errahmani ◽

Abdenour Laraba ◽

Hayet Mesbah ◽

...

Keyword(s):

Sunflower Oil ◽

Antioxidant Status ◽

Basal Diet ◽

Inflammatory Cells ◽

Significant Rise ◽

Control Group ◽

Histopathological Changes ◽

Group 5 ◽

Oxidant Effect ◽

Higher Doses

Abstract. The aim of this study is to evaluate the effect of α-tocopherol supplementation at two doses (600 and 1200 mg × kg–1) on kidney antioxidant status and the histopathological changes in Wistar rats after 12 weeks of exposure at different diets. Forty rats has been divided into 4 groups of 10 rats each, the control group received basal diet with 5 % fresh sunflower oil (FSO), the second group: 5 % oxidized sunflower oil (OSO), the third group: 5 % OSO supplemented with 600 mg × kg–1 α-tocopherol and the fourth group: 5 % OSO supplemented with 1200 mg × kg–1 α-tocopherol. In OSO groups, the results showed highly significant increases of LPO (from 31.3 ± 0.9 to 53.8 ± 1.2 nmol of MDA formed/min/mg protein, p < 0.0001) with a significant decrease (p < = 0.001) of the antioxidant enzymatic activities (CAT, SOD, GPX, GR and G6PDH), body weight (339 ± 9 to 290 ± 3 g) and α-tocopherol levels (13.6 ± 0.6 to 6.5 ± 0.4 μg/mg protein). In OSO groups with 600 mg × kg–1 α-tocopherol, an antioxidant effect was found, reflected by a return of the parameters to values similar to those of the control group. However, higher doses of α-tocopherol (1200 mg × kg–1) induced a depletion of antioxidant status, α-tocopherol levels (6.0 ± 0.3 μg/mg protein, p < 0.001) and a very highly significant rise (p < 0.0001) of LPO content (54.86 ± 0.01 nmol of MDA formed/min/mg protein). The kidney tissues also showed changes in glomerular, severe inflammatory cells infiltration, and formation of novel vessels. So, we can conclude that the oxidative stress is attenuated by a moderate administration of 600 mg × kg–1 α-tocopherol, while a pro-oxidant effect occurs at 1200 mg × kg–1 α-tocopherol.

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