scholarly journals Template free solvothermal synthesis of single crystal magnetic Fe 3 O 4 hollow spheres, their interaction with bovine serum albumin and antibacterial activities

2018 ◽  
Vol 22 (5) ◽  
pp. 569-580 ◽  
Author(s):  
M. Karuppuraja ◽  
S. Murugesan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Single Crystal ◽  
Bovine Serum ◽  
Solvothermal Synthesis ◽  
Hollow Spheres ◽  
Antibacterial Activities ◽  
Template Free

One-pot template-free synthesis of water-dispersive Fe3O4@C nanoparticles for adsorption of bovine serum albumin

2013 ◽  
Vol 37 (11) ◽  
pp. 3731 ◽  
Author(s):  
Zhiming Chen ◽  
Zhenglian Xue ◽  
Lin Chen ◽  
Zhirong Geng ◽  
Renchun Yang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
One Pot ◽  
Template Free

scholarly journals A Study of the Antibacterial Activities and the Mode of Action of L-Methionine and L-Cystine Based Surfactants and their Interaction with Bovine Serum Albumin Using Fluorescence Spectroscopy and in Silico Modelling

2021 ◽  
Vol 12 (6) ◽  
pp. 7356-7375
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Hydrophobic Interactions ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Resistant Bacteria ◽  
Binding Studies ◽  
Gram Positive ◽  
Gram Negative

Surfactants are versatile excipients that are commonly employed in diverse pharmaceutical formulations given their broad range of antimicrobial activities. Sulfur-based amino acid surfactants are promising candidates as substitutes for conventional antibacterial agents in light of the current antibiotic resistance crisis. Dodecyl esters of the free amine (SURF1), cationic ester hydrochloride (SURF2), and quaternary ammonium (QUAT3) derivatives of methionine, and the Gemini diester of cystine (GEM4) were synthesized and evaluated for antibacterial activity against Gram-positive and Gram-negative pathogens. All surfactants displayed moderate to high antibacterial activities, particularly on Gram-positive bacteria, with QUAT3 showing the highest activity. Among Gram-negative bacteria, QUAT3, GEM4, and SURF2 were mostly active towards K. pneumoniae with minimum inhibitory concentrations (MIC) ranging from 0.004 to 0.441 mM. QUAT3 and GEM4 displayed a bacteriostatic behavior similar to that of tetracycline, as assessed by broth macrodilution assays. Fluorescence binding studies with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) revealed that the antibacterial activities could be attributed to a combination of electrostatic and hydrophobic interactions. Bovine serum albumin (BSA) fluorescence and molecular docking studies indicated that SURF1 and QUAT3 interact mainly via van der Waals' forces and hydrogen bonding while SURF2 binds through hydrophobic interactions. QUAT3, which displays broad-spectrum activity, has the potential to combat drug-resistant bacteria.

Effect of bovine serum albumin on tartrate-modified manganese ferrite nano hollow spheres: spectroscopic and toxicity study

2019 ◽  
Vol 21 (20) ◽  
pp. 10726-10737 ◽  
Author(s):  
Indranil Chakraborty ◽  
Urmila Saha ◽  
Dipika Mandal ◽  
Suprabhat Mukherjee ◽  
Nikhilesh Joardar ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Blue Light ◽  
Fluorescence Intensity ◽  
Bovine Serum ◽  
Hollow Spheres ◽  
Toxicity Study ◽  
Light Irradiation ◽  
Microscopic Image ◽  
Manganese Ferrite

Green-colored fluorescence microscopic image of [BSA]–[T-MnFe2O4] under blue light irradiation along with higher fluorescence intensity of the same compared to T-MnFe2O4 NHSs.

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

1981 ◽  
Vol 46 (03) ◽  
pp. 645-647 ◽  
Author(s):  
M A Orchard ◽  
C Robinson
Keyword(s):  
Platelet Aggregation ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protective Effect ◽  
Whole Blood ◽  
Bovine Serum ◽  
Fatty Acid Content ◽  
Krebs Solution ◽  
Antiaggregatory Activity ◽  
Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

RADIOIMMUNOASSAY OF OESTRONE AND OESTRADIOL IN THE PERIPHERAL PLASMA OF THE DOMESTIC FOWL IN VARIOUS PHYSIOLOGICAL STATES AND OF HYPOPHYSECTOMIZED AND OVARIECTOMIZED FOWLS

1974 ◽  
Vol 75 (1) ◽  
pp. 133-140 ◽  
Author(s):  
B. E. Senior
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Diethyl Ether ◽  
Bovine Serum ◽  
Domestic Fowl ◽  
Laying Hens ◽  
Peripheral Plasma ◽  
The Mean ◽  
Precision And Accuracy ◽  
Chicken Ovary

ABSTRACT A radioimmunoassay was developed to measure the levels of oestrone and oestradiol in 0.5–1.0 ml of domestic fowl peripheral plasma. The oestrogens were extracted with diethyl ether, chromatographed on columns of Sephadex LH-20 and assayed with an antiserum prepared against oestradiol-17β-succinyl-bovine serum albumin using a 17 h incubation at 4°C. The specificity, sensitivity, precision and accuracy of the assays were satisfactory. Oestrogen concentrations were determined in the plasma of birds in various reproductive states. In laying hens the ranges of oestrone and oestradiol were 12–190 pg/ml and 29–327 pg/ml respectively. Levels in immature birds, in adult cockerels and in an ovariectomized hen were barely detectable. The mean concentrations of oestrone and oestradiol in the plasma of four non-laying hens (55 pg/ml and 72 pg/ml respectively) and one partially ovariectomized hen (71 pg/ml and 134 pg/ml respectively) were well within the range for laying hens. It is evident that the large, yolk-filled follicles are not the only source of oestrogens in the chicken ovary.

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