scholarly journals Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum

2014 ◽  
Vol 8 (3) ◽  
pp. 216-224 ◽  
Author(s):  
Faten Medini ◽  
Hanen Fellah ◽  
Riadh Ksouri ◽  
Chedly Abdelly
Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2746
Author(s):  
Giuseppe Squillaci ◽  
Carla Zannella ◽  
Virginia Carbone ◽  
Paola Minasi ◽  
Veronica Folliero ◽  
...  

The purpose of the current study was to determine the phenolic composition, antioxidant, and antimicrobial activities in grape cane extracts from typical cultivars of Southern Italy. Aqueous extracts at different pHs (1–13) were prepared from “Aglianico”, “Fiano”, and “Greco” grape canes. The results demonstrated that an alkaline pH (13.00) produced the best polyphenol-rich extracts, as the total phenolic content was more than double when compared to the respective extracts prepared at pH 1.00. “Greco” grape canes gave the highest quantity of phenolic compounds at each pH, ranging from 42.7 ± 0.4 to 104.3 ± 3.0 mg Gallic Acid Equivalents (GAE)/g Dry Extract (DE) from pH 1.00 to 13.00. The Radical Scavenging Activity (RSA) and the Ferric Reducing Antioxidant Power (FRAP) were measured. The highest antioxidant activity was showed by “Greco” extract at pH 7.00. Seventy-five compounds were identified in the extracts by HPLC-MS with six of them described for the first time in grape canes. Procyanidins were highly abundant in extracts at pH 7.00, whereas stilbenoids were the most represented compounds at pH 13.00. Very strong antiviral activity against herpes simplex viruses was recorded for the extracts at pH 7.00 and 13.00 that were active in the early stages of infection by acting directly against the viral particles. The overall results suggest that grape canes, currently underutilized, can be usefully valorised by providing active extracts to use as antioxidant and antiviral agents.


2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Alper Gökbulut ◽  
Onural Özhana ◽  
Basri Satılmiş ◽  
Kadir Batçioğlu ◽  
Selami Günal ◽  
...  

Three Inula species, I. viscosa, I. helenium ssp. turcoracemosa and I. montbretiana, collected from different locations of Anatolia were investigated for their antioxidant and antimicrobial potential, and their total phenolic content and phenolic composition. Antioxidant activities of various extracts of the plant parts were measured using DPPH radical scavenging and ABTS assays. Antimicrobial potential of methanol extracts of the plant parts was determined by the agar dilution method against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Candida tropicalis. All the extracts were more active against Gram-positive bacteria and yeasts than Gram-negative bacteria. The extracts exhibited antioxidant and antimicrobial activities in different concentrations. Total phenolic concentration of the extracts was estimated with Folin-Ciocalteu reagent using gallic acid as standard. The total phenolic content varied widely in different parts of the three tested Inula species, ranging from 21.1 ± 0.8 to 190.9 ± 6.1 mg GAE/g extract. Phenolic components, such as chlorogenic acid, caffeic acid, rutin, myricetin, quercetin, luteolin and kaempferol were quantified by HPLC-DAD in the methanol extracts of the Inula species. It was obvious that the antioxidant and antimicrobial properties of the plants were due to the phenolics.


2021 ◽  
Vol 45 (2) ◽  
pp. 223-231
Author(s):  
Kara Guzel ◽  
Mahmut Ulger ◽  
Ahmet Kahraman

The phytochemical contents of Salvia virgata mericarps and the antioxidant and antimicrobial activities of its ethanol extract were studied for the first time. S. virgata mericarps were collected in the province of Trabzon, Turkey. Chemical analyses were performed using chromatographic methods. The total phenolic and flavonoid contents and antioxidant potential were measured using Folin-Ciocalteu, Al(NO3)3, and DPPH radical scavenging activity methods, respectively. The antimicrobial activity was evaluated using the microdilution method for all the tested bacterial and fungal strains, with the exception of Mycobacterium tuberculosis for which the resazurin microtiter plate method was applied. The mericarps were rich in glutamic acid (3934 mg/100 g), potassium (12578.8 ?g/g), calcium (12092.0 ?g/g), and dietary fibers (35.565 g/100 g). The total phenolic and flavonoid contents were 2.50 ?g GAE/mg extract and 0.34 ?g QE/mg extract, respectively. The most effective DPPH free radical scavenging activity determined for the highest applied concentration was 92.44%. The ethanol extract obtained from the mericarps was found to be as effective as the reference drug ampicillin (MIC value = 125 ?g/mL) against the nosocomial pathogen Acinetobacter baumannii. In conclusion, S. virgata mericarps provide good nutritional value with low amounts of carbohydrates and high dietary fibers, amino acids, minerals and total phenolic and flavonoid contents and medicinal properties.


2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Bhanuz Dechayont ◽  
Pimnapa Ruamdee ◽  
Sukrita Poonnaimuang ◽  
Khwanchanok Mokmued ◽  
Jitpisute Chunthorng-Orn

Pogostemon cablin (Lamiaceae) has been widely used in traditional medicine. In this study, the antioxidant and antimicrobial activities of leaves from P. cablin extracts were investigated. The water extracts had the highest total phenolic content 116.88±0.48 mg gallic acid equivalent/g of dry plant extract. Nevertheless, high levels of total flavonoid content were found in ethanolic extracts 280.12±2.04 mg quercetin equivalent/g of dry plant extract. The highest antioxidant activities were found for the ethanolic extract (IC50=18±0.90, 20±0.24 μg/mL) by DPPH and ABTS scavenging assays, respectively. Both extracts showed moderate inhibition of superoxide inhibition (O2∙-) and nitric oxide (NO) production in concentration-dependent manner. Antibacterial activity was calculated by disk diffusion, minimum inhibitory concentration (MIC), and minimum bacterial concentration (MBC). The ethanolic extract had the greatest activity against methicillin resistant Staphylococcus aureus, methicillin sensitive S. aureus, and Streptococcus pyogenes with zone diameters of 11.67±1.53, 10.33±2.52, and 10.33±1.15 mm, respectively. The corresponding MIC and MBCs were 5, 0.625, and 0.039 mg/mL. P. cablin extracts contain antioxidant and antibacterial properties that should be exploited for possible clinical application.


Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 178
Author(s):  
Vanessa Silva ◽  
Rupesh Kumar Singh ◽  
Nelson Gomes ◽  
Bruno Gonçalves Soares ◽  
Adriana Silva ◽  
...  

Chitosan, a natural polysaccharide, has been previously proposed as an elicitor in plants to prevent pathogen infections. The present study aimed to analyze the effect of chitosan solution and chitosan nanoparticles treatment applied on the grapevine variety Sousão with respect to the phenolic composition, antioxidant potential and antibacterial activity of its individual grape components. Grapevine plants of selected lines were sprayed with chitosan solution and chitosan nanoparticles, and ethanolic extracts of stems, seeds and skins were prepared from grapevines treated and not treated with chitosan. Total phenolic, anthocyanin and tannin contents were studied, and the identification of the individual phenolic compounds was performed by HPLC-DAD. The antimicrobial susceptibility method was performed using the Kirby-Bauer disc diffusion method against multidrug-resistant bacteria. Overall, there was small increase in the concentration of phenolic compounds, antioxidant and antimicrobial activities in grape components treated with chitosan solution. Seed extracts showed the highest antioxidant and antimicrobial activities. The studied individual components obtained from chitosan-treated grapevines could represent an added value due to the increased antioxidant and antibacterial potentials. The phenolic compounds found in components may be used in food and pharmaceutical industries as natural food preservers and antibiotic adjuvants.


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