In situ ruminal amino acid degradability of green and ensiled Italian rye-grass according to particle transit model and microbial contamination correction

2009 ◽  
Vol 123 (2-3) ◽  
pp. 209-214 ◽  
Author(s):  
J. González ◽  
C. Centeno ◽  
N. Morujo ◽  
J. Faría-Mármol ◽  
A. Martínez
2013 ◽  
Vol 94 (7) ◽  
pp. 1291-1298 ◽  
Author(s):  
Javier González ◽  
José M Arroyo ◽  
Rabiaa Mouhbi ◽  
Jesús A Guevara-González ◽  
Vicente J Moya ◽  
...  

1990 ◽  
Vol 38 (4) ◽  
pp. 719-724
Author(s):  
G. Hof ◽  
W.J.A. Kouwenberg ◽  
S. Tamminga

Soyabean meal and wilted grass silage were suspended in nylon bags in the rumen for 0, 3, 6 and 12 h and 0, 6, 24 and 48 h, respectively. Samples were then washed using the standard procedure, or standard washing, followed by soaking for 16 h in neutral detergent at 70 or 25 degrees C, followed by standard washing. The amino acid profile of the samples was determined by amino acid content in the original feed. Amino acids disappeared at a faster rate than non-amino acid N. Microbial contamination was only seen in the grass silage and its residues after incubation. None of the washing procedures effectively removed microbial contamination. (Abstract retrieved from CAB Abstracts by CABI’s permission)


Function ◽  
2021 ◽  
Author(s):  
Bruce R Stevens ◽  
J Clive Ellory ◽  
Robert L Preston

Abstract The SARS-CoV-2 receptor, Angiotensin Converting Enzyme-2 (ACE2), is expressed at levels of greatest magnitude in the small intestine as compared to all other human tissues. Enterocyte ACE2 is co-expressed as the apical membrane trafficking partner obligatory for expression and activity of the B0AT1 sodium-dependent neutral amino acid transporter. These components are assembled as an [ACE2: B0AT1]2 dimer-of-heterodimers quaternary complex that putatively steers SARS-CoV-2 tropism in the gastrointestinal (GI) tract. GI clinical symptomology is reported in about half of COVID-19 patients, and can be accompanied by gut shedding of virion particles. We hypothesized that within this 4-mer structural complex, each [ACE2: B0AT1] heterodimer pair constitutes a physiological “functional unit.” This was confirmed experimentally by employing purified lyophilized enterocyte brush border membrane vesicles that were exposed to increasing doses of high-energy electron radiation from a 16 MeV linear accelerator. Based on established target theory, the results indicated the presence of Na+-dependent neutral amino acid influx transport activity functional unit with target size mw = 183.7 ± 16.8 kDa in situ in intact apical membranes. Each thermodynamically stabilized [ACE2: B0AT1] heterodimer functional unit manifests the transport activity within the whole ∼345 kDa [ACE2: B0AT1]2 dimer-of-heterodimers quaternary structural complex. The results are consistent with our prior molecular docking modeling and gut-lung axis approaches to understanding COVID-19. These findings advance the understanding of the physiology of B0AT1 interaction with ACE2 in the gut, and thereby potentially contribute to translational developments designed to treat or mitigate COVID-19 variant outbreaks and/or GI symptom persistence in long-haul Post-Acute Sequelae of SARS-CoV-2 (PASC).


2016 ◽  
Vol 14 (2) ◽  
pp. 556-563 ◽  
Author(s):  
Veladi Panduranga ◽  
Girish Prabhu ◽  
Roopesh Kumar ◽  
Basavaprabhu Basavaprabhu ◽  
Vommina V. Sureshbabu

A simple and efficient method for the synthesis of N,N’-orthogonally protected imide tethered peptidomimetics is presented. The imide peptidomimetics were synthesized by coupling the in situ generated selenocarboxylate of Nα-protected amino acids with Nα-protected amino acid azides in good yields.


1977 ◽  
Vol 14 (12) ◽  
pp. 2824-2857 ◽  
Author(s):  
G. H. Miller ◽  
J. T. Andrews ◽  
S. K. Short

A study of the stratigraphic sequence (14C and amino acid age control), marine bivalve faunal changes, and palynology of buried soils and organic-rich sediment collected from the Clyde Foreland Formation in the extensive cliff sections of the Clyde foreland, eastern Baffin Island, N.W.T., suggests the following last interglacial – Foxe (last glaciation) glacial – present interglacial sequence.(1) Cape Christian Member (ca. 130 000 years BP?)Consists of the Sledgepointer till overlain by the Cape Christian marine sediments. In situ molluscan fauna, collected from the marine sediments, contain a moderately warm bivalve assemblage. A well-developed soil that formed on the marine sediments (Cape Christian soil) contains an interglacial pollen assemblage dominated by dwarf birch. U-series dates of > 115 000 and ca. 130 000 years BP on molluscs from the Cape Christian marine sediments suggest that they were deposited during the last interglaciation, here termed the Cape Christian Interglaciation. The development of a subarctic pollen assemblage in the Cape Christian soil has not been duplicated during the present interglaciation, suggesting higher summer temperatures and perhaps a duration well in excess of 10 000 years for the last interglaciation.(2) Kuvinilk MemberConsists of fossiliferous marine sediments, locally divided by the Clyde till into upper and lower units. The Clyde till was deposited by the earliest and most extensive advance of the Foxe (last) Glaciation. Kuvinilk marine sediments both under- and overlying the Clyde till contain the pecten Chlamys islandicus, indicating that the outlet glacier advanced into a subarctic marine environment. Amino acid ratios from in situ pelecypod shells abovę and below the Clyde till are not statistically different, but contrast markedly with ratios obtained from the same species in the Cape Christian Member. Organic horizons within the Kuvinilk marine sediments contain a relatively rich pollen assemblage, although 'absolute' counts are low.(3) Kogalu Member (> 35 00014C years BP)Sediments of the Kogalu Member unconformably overlie those of the Kuvinilk Member, but are of a similar character. The dominant sediments are marine in origin, but in places are divided into upper and lower units by the Ayr Lake till. Amino acid ratios from in situ shells above and below the Ayr Lake till are indistinguishable, but substantially less than those in the Kuvinilk Member, suggesting the two members are separated by a considerable time interval. Radiocarbon dates on shells in the Kogalu marine sediments range from 33 000 to 47 700 years BP, but these may be only minimum estimates. The sea transgressed to a maximum level 70–80 m asl, coincident with the glacial maximum. Subarctic marine fauna of interstadial–interglacial character occur within the Kogalu marine sediments.(4) Eglinton Member (10 000 years BP to present)A major unconformity exists between the Kogalu and Eglinton Members. Ravenscraig marine sediments were deposited during an early Holocene marine transgression–regression cycle; the oldest dates on these sediments are ca. 10 000 years BP. Locally a vegetation mat occurs at the base or within the Ravenscraig unit. Pollen from these beds is sparse, but indicates a terrestrial vegetation assemblage as diverse as that of today. There is no evidence that Laurentide Ice reached the foreland during the last 30 000 years. Eolian sands that overlie a soil developed on the marine sediments record a late Holocene climatic deterioration. Pollen in organic-rich sediments at the base of, and within, the eolian sands record a vegetation shift in response to climatic change.


Synlett ◽  
2017 ◽  
Vol 28 (15) ◽  
pp. 1984-1989 ◽  
Author(s):  
Rudolf Schneider ◽  
Stephan Schmidt ◽  
Sven Hanelt ◽  
Carsten Canitz ◽  
Holger Hoffmann ◽  
...  

For many heterogeneous sensor applications as well as the synthesis of hapten antigens to produce antibodies, protein conjugates of the target substance are essential. A requirement is that the target substance already offers or is modified to contain a functionality that allows for coupling to a protein, that is, an amino acid residue. Ideally, to avoid shielding of the compound by the carrier protein, a sufficient distance to the protein surface should be provided. With its carboxyl function diclofenac (DCF) allows for direct binding to lysine residues after in situ synthesis of the NHS ester. One problem is that diclofenac as free acid tends to autocondensation, which results in low yields. Here we describe the ‘insertion’ of a C6 spacer via synthesis of the amide with 6-aminohexanoic acid. To carry out the reaction in solution, first the methyl ester of the amino acid had to be produced. Due to otherwise low yields and large cleaning efforts, solid-phase synthesis on Fmoc Ahx Wang resin is recommended. The crude product is mainly contaminated by cleavage products from the resin which were removed by chromatography. The structure of the highly pure hapten was completely determined by nuclear magnetic resonance (NMR) spectroscopy.


1980 ◽  
Vol 188 (1) ◽  
pp. 269-278 ◽  
Author(s):  
Clyde A. Watkins ◽  
D. Eugene Rannels

Compartmentalization of amino acid was investigated to define conditions required for accurate measurements of rates of protein synthesis in rat lungs perfused in situ. Lungs were perfused with Krebs–Henseleit bicarbonate buffer containing 4.5% (w/v) bovine serum albumin, 5.6mm-glucose, normal plasma concentrations of 19 amino acids, and 8.6–690μm-[U-14C]phenylalanine. The perfusate was equilibrated with the same humidified gas mixture used to ventilate the lungs [O2/CO2 (19:1) or O2/N2/CO2 (4:15:1)]. [U-14C]Phenylalanine was shown to be a suitable precursor for studies of protein synthesis in perfused lungs: it entered the tissue rapidly (t½, 81s) and was not converted to other compounds. As perfusate phenylalanine was decreased below 5 times the normal plasma concentration, the specific radioactivity of the pool of phenylalanine serving as precursor for protein synthesis, and thus [14C]phenylalanine incorporation into protein, declined. In contrast, incorporation of [14C]histidine into lung protein was unaffected. At low perfusate phenylalanine concentrations, rates of protein synthesis that were based on the specific radioactivity of phenylalanyl-tRNA were between rates calculated from the specific radioactivity of phenylalanine in the extracellular or intracellular pools. Rates based on the specific radioactivities of these three pools of phenylalanine were the same when extracellular phenylalanine was increased. These observations suggested that: (1) phenylalanine was compartmentalized in lung tissue; (2) neither the extracellular nor the total intracellular pool of phenylalanine served as the sole source of precursor for protein; (3) at low extracellular phenylalanine concentrations, rates of protein synthesis were in error if calculated from the specific radioactivity of the free amino acid; (4) at high extracellular phenylalanine concentrations, the effects of compartmentalization were negligible and protein synthesis could be calculated accurately from the specific radioactivity of the free or tRNA-bound phenylalanine pool.


2019 ◽  
Author(s):  
Moritz Senger ◽  
Viktor Eichmann ◽  
Konstantin Laun ◽  
Jifu Duan ◽  
Florian Wittkamp ◽  
...  

Hydrogenases are metalloenzymes that catalyse the interconversion of protons and molecular hydrogen, H2. [FeFe]-hydrogenases show particularly high rates of hydrogen turnover and have inspired numerous compounds for biomimetic H2 production. Two decades of research on the active site cofactor of [FeFe]-hydrogenases have put forward multiple models of the catalytic proceedings. In comparison, understanding of the catalytic proton transfer is poor. We were able to identify the amino acid residues forming a proton transfer pathway between active site cofactor and bulk solvent; however, the exact mechanism of catalytic proton transfer remained inconclusive. Here, we employ in situ IR difference spectroscopy on the [FeFe]-hydrogenase from Chlamydomonas reinhardtii evaluating dynamic changes in the hydrogen-bonding network upon catalytic proton transfer. Our analysis allows for a direct, molecular unique assignment to individual amino acid residues. We found that transient protonation changes of arginine and glutamic acid residues facilitate bidirectional proton transfer in [FeFe]-hydrogenases.<br>


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