Quantification of microplastics along the Caribbean Coastline of Colombia: Pollution profile and biological effects on Caenorhabditis elegans

2019 ◽  
Vol 146 ◽  
pp. 574-583 ◽  
Author(s):  
Isabel Acosta-Coley ◽  
Margareth Duran-Izquierdo ◽  
Erika Rodriguez-Cavallo ◽  
Jairo Mercado-Camargo ◽  
Dario Mendez-Cuadro ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Biological Effects ◽  
The Caribbean ◽  
Pollution Profile

Study of Zalema Grape Pomace: Phenolic Composition and Biological Effects in Caenorhabditis elegans

2013 ◽  
Vol 61 (21) ◽  
pp. 5114-5121 ◽  
Author(s):  
M. José Jara-Palacios ◽  
Susana González-Manzano ◽  
M. Luisa Escudero-Gilete ◽  
Dolores Hernanz ◽  
Montserrat Dueñas ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Biological Effects ◽  
Grape Pomace ◽  
Phenolic Composition

scholarly journals Review of Biological Effects of Acute and Chronic Radiation Exposure on Caenorhabditis elegans

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1966
Author(s):  
Rabin Dhakal ◽  
Mohammad Yosofvand ◽  
Mahsa Yavari ◽  
Ramzi Abdulrahman ◽  
Ryan Schurr ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Ionizing Radiation ◽  
Biological Effects ◽  
Model Organism ◽  
Model Organisms ◽  
C Elegans ◽  
Chronic Radiation ◽  
Biological Responses ◽  
Chronic Radiation Exposure ◽  
Acute And Chronic Exposure

Knowledge regarding complex radiation responses in biological systems can be enhanced using genetically amenable model organisms. In this manuscript, we reviewed the use of the nematode, Caenorhabditis elegans (C. elegans), as a model organism to investigate radiation’s biological effects. Diverse types of experiments were conducted on C. elegans, using acute and chronic exposure to different ionizing radiation types, and to assess various biological responses. These responses differed based on the type and dose of radiation and the chemical substances in which the worms were grown or maintained. A few studies compared responses to various radiation types and doses as well as other environmental exposures. Therefore, this paper focused on the effect of irradiation on C. elegans, based on the intensity of the radiation dose and the length of exposure and ways to decrease the effects of ionizing radiation. Moreover, we discussed several studies showing that dietary components such as vitamin A, polyunsaturated fatty acids, and polyphenol-rich food source may promote the resistance of C. elegans to ionizing radiation and increase their life span after irradiation.

scholarly journals Flavonoids’ Effects on Caenorhabditis elegans’ Longevity, Fat Accumulation, Stress Resistance and Gene Modulation Involve mTOR, SKN-1 and DAF-16

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 438
Author(s):  
María Alejandra Guerrero-Rubio ◽  
Samanta Hernández-García ◽  
Francisco García-Carmona ◽  
Fernando Gandía-Herrero
Keyword(s):  
Caenorhabditis Elegans ◽  
Stress Resistance ◽  
Inflammatory Diseases ◽  
Biological Effects ◽  
Molecular Targets ◽  
Mechanistic Studies ◽  
C Elegans ◽  
Nutraceutical Compounds ◽  
Age Related ◽  
Health Promoting

Flavonoids are potential nutraceutical compounds present in diary food. They are considered health-promoting compounds and promising drugs for different diseases, such as neurological and inflammatory diseases, diabetes and cancer. Therefore, toxicological and mechanistic studies should be done to assert the biological effects and identify the molecular targets of these compounds. In this work we describe the effects of six structurally-related flavonoids—baicalein, chrysin, scutellarein, 6-hydroxyflavone, 6,7-dihydroxyflavone and 7,8-dihydroxyflavone—on Caenorhabditis elegans’ lifespan and stress resistance. The results showed that chrysin, 6-hydroxyflavone and baicalein prolonged C. elegans’ lifespan by up to 8.5%, 11.8% and 18.6%, respectively. The lifespan extensions caused by these flavonoids are dependent on different signaling pathways. The results suggested that chrysin’s effects are dependent on the insulin signaling pathway via DAF-16/FOXO. Baicalein and 6-hydroxyflavone’s effects are dependent on the SKN-1/Nfr2 pathway. In addition, microarray analysis showed that baicalein downregulates important age-related genes, such as mTOR and PARP.

scholarly journals Biological effects, translocation, and metabolism of quantum dots in the nematode Caenorhabditis elegans

2016 ◽  
Vol 5 (4) ◽  
pp. 1003-1011 ◽  
Author(s):  
Dayong Wang
Keyword(s):  
Quantum Dots ◽  
Caenorhabditis Elegans ◽  
Recent Progress ◽  
Biological Effects ◽  
Assay System ◽  
Nematode Caenorhabditis Elegans ◽  
C Elegans

We summarize recent progress on the biological effects, translocation, and metabolism of QDs in thein vivoassay system ofC. elegans.

scholarly journals Assessment of the In Vivo Antioxidant Activity of an Anthocyanin-Rich Bilberry Extract Using the Caenorhabditis elegans Model

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 509
Author(s):  
Ana M. González-Paramás ◽  
Virginia Brighenti ◽  
Laura Bertoni ◽  
Laura Marcelloni ◽  
Begoña Ayuda-Durán ◽  
...  
Keyword(s):  
Thermal Stress ◽  
Caenorhabditis Elegans ◽  
Biological Effects ◽  
In Vivo Model ◽  
Heat Shock Factor 1 ◽  
Formation Factor ◽  
C Elegans ◽  
Mutant Strains ◽  
Bilberry Extract

Anthocyanins have been associated with several health benefits, although the responsible mechanisms are not well established yet. In the present study, an anthocyanin-rich extract from bilberry (Vaccinium myrtillus L.) was tested in order to evaluate its capacity to modulate reactive oxygen species (ROS) production and resistance to thermally induced oxidative stress, using the nematode Caenorhabditis elegans as an in vivo model. The assays were carried out with the wild-type N2 strain and the mutant strains daf-16(mu86) I and hsf-1(sy441), which were grown in the presence of two anthocyanin extract concentrations (5 and 10 μg/mL in the culture medium) and further subjected to thermal stress. The treatment with the anthocyanin extract at 5 μg/mL showed protective effects on the accumulation of ROS and increased thermal resistance in C. elegans, both in stressed and non-stressed young and aged worms. However, detrimental effects were observed in nematodes treated with 10 μg/mL, leading to a higher worm mortality rate compared to controls, which was interpreted as a hormetic response. These findings suggested that the effects of the bilberry extract on C. elegans might not rely on its direct antioxidant capacity, but other mechanisms could also be involved. Additional assays were performed in two mutant strains with loss-of-function for DAF-16 (abnormal DAuer Formation factor 16) and HSF-1 (Heat Shock Factor 1) transcription factors, which act downstream of the insulin/insulin like growth factor-1 (IGF-1) signaling pathway. The results indicated that the modulation of these factors could be behind the improvement in the resistance against thermal stress produced by bilberry anthocyanins in young individuals, whereas they do not totally explain the effects produced in worms in the post-reproductive development stage. Further experiments are needed to continue uncovering the mechanisms behind the biological effects of anthocyanins in living organisms, as well as to establish whether they fall within the hormesis concept.

scholarly journals Caenorhabditis elegans Exhibits Positive Gravitaxis

2019 ◽  
Author(s):  
Wei-Long Chen ◽  
Hungtang Ko ◽  
Han-Sheng Chuang ◽  
Haim H. Bau ◽  
David Raizen
Keyword(s):  
Caenorhabditis Elegans ◽  
Molecular Mechanisms ◽  
Biological Effects ◽  
Neural Mechanisms ◽  
Mechanistic Studies ◽  
Active Behavior ◽  
C Elegans ◽  
Sensory Cilia ◽  
Hydrodynamic Effects ◽  
Nematode Ecology

AbstractWhether or not the micro swimmer Caenorhabditis elegans senses and respond to gravity is unknown. We find that C. elegans aligns its swimming direction with that of the gravity vector (positive gravitaxis). When placed in an aqueous solution that is denser than the animals, they still orient downwards, indicating that non-uniform mass distribution and/or hydrodynamic effects are not responsible for animal’s downward orientation. Paralyzed worms and worms with globally disrupted sensory cilia do not change orientation as they settle in solution, indicating that gravitaxis is an active behavior that requires gravisensation. Other types of sensory driven orientation behaviors cannot explain our observed downward orientation. Like other neural behaviors, the ability to respond to gravity declines with age. Our study establishes gravitaxis in the micro swimmer C. elegans and suggests that C. elegans can be used as a genetically tractable system to study molecular and neural mechanisms of gravity sensing and orientation.Significance StatementUnderstanding how animals respond to gravity is not only of fundamental scientific interest, but has clinical relevance, given the prevalence of postural instability in aged individuals. Determining whether C. elegans responds to gravity is important for mechanistic studies of gravity sensing in an experimentally tractable animal, for a better understanding of nematode ecology and evolution, and for studying biological effects of microgravity. Our experiments, which indicate that C. elegans senses and responds to gravity, set the stage for mechanistic studies on molecular mechanisms of gravity sensing.

scholarly journals Exploring Target Genes Involved in the Effect of Quercetin on the Response to Oxidative Stress in Caenorhabditis elegans

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 585 ◽  
Author(s):  
Begoña Ayuda-Durán ◽  
Susana González-Manzano ◽  
Antonio Miranda-Vizuete ◽  
Eva Sánchez-Hernández ◽  
Marta R. Romero ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Fluorescent Protein ◽  
Biological Activities ◽  
Biological Effects ◽  
Knockout Mutant ◽  
C Elegans

Quercetin is one the most abundant flavonoids in the human diet. Although it is well known that quercetin exhibits a range of biological activities, the mechanisms behind these activities remain unresolved. The aim of this work is to progress in the knowledge of the molecular mechanisms involved in the biological effects of quercetin using Caenorhabditis elegans as a model organism. With this aim, the nematode has been used to explore the ability of this flavonoid to modulate the insulin/insulin-like growth factor 1(IGF-1) signaling pathway (IIS) and the expression of some genes related to stress response. Different methodological approaches have been used, i.e., assays in knockout mutant worms, gene expression assessment by RT-qPCR, and C. elegans transgenic strains expressing green fluorescent protein (GFP) reporters. The results showed that the improvement of the oxidative stress resistance of C. elegans induced by quercetin could be explained, at least in part, by the modulation of the insulin signaling pathway, involving genes age-1, akt-1, akt-2, daf-18, sgk-1, daf-2, and skn-1. However, this effect could be independent of the transcription factors DAF-16 and HSF-1 that regulate this pathway. Moreover, quercetin was also able to increase expression of hsp-16.2 in aged worms. This observation could be of particular interest to explain the effects of enhanced lifespan and greater resistance to stress induced by quercetin in C. elegans, since the expression of many heat shock proteins diminishes in aging worms.

Bioavailability and Biological Effects of 2-O-β-d-Glucopyranosyl-carboxyatractyligenin from Green Coffee in Caenorhabditis elegans

2019 ◽  
Vol 67 (17) ◽  
pp. 4774-4781
Author(s):  
Britta Spanier ◽  
Roman Lang ◽  
Daniela Weber ◽  
Anica Lechner ◽  
Tizia Thoma ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Biological Effects ◽  
Green Coffee

Antioxidant Activities of the Pyroligneous Acid in Living Caenorhabditis Elegans

2011 ◽  
Vol 236-238 ◽  
pp. 2564-2569 ◽  
Author(s):  
Ke Zhou Cai ◽  
Yu Jie He
Keyword(s):  
Ascorbic Acid ◽  
Caenorhabditis Elegans ◽  
Cell Apoptosis ◽  
Brood Size ◽  
Biological Effects ◽  
Control Level ◽  
Alpha Tocopherol ◽  
Germline Cell ◽  
Chemical Examination ◽  
Pyroligneous Acid

Pyroligneous acid (PA), obtained from charcoal production, has been reported excellent capability in antioxidant by chemical examination. However, the biological effect of PA in living animal is still unknown. In this study, a simple model organism, the nematode Caenorhabditis elegans, was used as an in vivo system to assess the biological effects of PA treatment. The worms were exposed to concentrated pyroligneous acid (CPA) and extraction (CPAE) thereof in a 0.5-5.0 mg/mL concentration and their brood size and germline cell apoptotisis were examined. The results showed that CPA and CPAE rescued the germline cell apoptosis induced by paraquat, a reactive oxygen species (ROS) generator. Additionally, CPA and CPAE did not show negative effect on the brood size and germline cell apoptosis in wild type at normal culture condition, suggesting safety of PA in living worms. To further scrutinize the antioxidant ability of PA, the apoptotic cell of germline induced by parapuat was assayed after treatment of 0.5 mg/mL CPA, CPAE, L-ascorbic acid and alpha-tocopherol. Treatment with CPAE or L-ascorbic acid could almost completely rescue the germline apoptosis to control level, indicating a potential material in food and biomedicine of PA.

UV-induced over time transformation of AgNPs in commercial wound dressings and adverse biological effects on Caenorhabditis elegans

NanoImpact ◽  
2020 ◽  
Vol 17 ◽  
pp. 100193
Author(s):  
Yaguang Nie ◽  
Jingjing Wang ◽  
Hui Dai ◽  
Juan Wang ◽  
Mudi Wang ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Biological Effects ◽  
Wound Dressings ◽  
Time Transformation ◽  
Over Time
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