Antioxidant Activities of the Pyroligneous Acid in Living Caenorhabditis Elegans

2011 ◽  
Vol 236-238 ◽  
pp. 2564-2569 ◽  
Author(s):  
Ke Zhou Cai ◽  
Yu Jie He
Keyword(s):  
Ascorbic Acid ◽  
Caenorhabditis Elegans ◽  
Cell Apoptosis ◽  
Brood Size ◽  
Biological Effects ◽  
Control Level ◽  
Alpha Tocopherol ◽  
Germline Cell ◽  
Chemical Examination ◽  
Pyroligneous Acid

Pyroligneous acid (PA), obtained from charcoal production, has been reported excellent capability in antioxidant by chemical examination. However, the biological effect of PA in living animal is still unknown. In this study, a simple model organism, the nematode Caenorhabditis elegans, was used as an in vivo system to assess the biological effects of PA treatment. The worms were exposed to concentrated pyroligneous acid (CPA) and extraction (CPAE) thereof in a 0.5-5.0 mg/mL concentration and their brood size and germline cell apoptotisis were examined. The results showed that CPA and CPAE rescued the germline cell apoptosis induced by paraquat, a reactive oxygen species (ROS) generator. Additionally, CPA and CPAE did not show negative effect on the brood size and germline cell apoptosis in wild type at normal culture condition, suggesting safety of PA in living worms. To further scrutinize the antioxidant ability of PA, the apoptotic cell of germline induced by parapuat was assayed after treatment of 0.5 mg/mL CPA, CPAE, L-ascorbic acid and alpha-tocopherol. Treatment with CPAE or L-ascorbic acid could almost completely rescue the germline apoptosis to control level, indicating a potential material in food and biomedicine of PA.

scholarly journals Effects of Manganese Porphyrins on Cellular Sulfur Metabolism

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 980 ◽  
Author(s):  
Kenneth R. Olson ◽  
Yan Gao ◽  
Andrea K. Steiger ◽  
Michael D. Pluth ◽  
Charles R. Tessier ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Biological Effects ◽  
Sulfur Metabolism ◽  
Hek293 Cells ◽  
Redox Cycle ◽  
Major Site ◽  
Manganese Porphyrins ◽  
Short And Long Term ◽  
H2s Production

Manganese porphyrins (MnPs), MnTE-2-PyP5+, MnTnHex-2-PyP5+ and MnTnBuOE-2-PyP5+, are superoxide dismutase (SOD) mimetics and form a redox cycle between O2 and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H2O2). We previously found that MnPs oxidize hydrogen sulfide (H2S) to polysulfides (PS; H2Sn, n = 2–6) in buffer. Here, we examine the effects of MnPs for 24 h on H2S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H2S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H2S production and increased intracellular PS. H2S metabolism and PS production were unaffected by cellular O2 (5% versus 21% O2), H2O2 or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H2S production in HEK293 cells and that MnPs decrease mitochondrial H2S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H2S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism.

scholarly journals Ascorbic acid, alpha-tocopherol, and oregano supplements reduce stress-induced deterioration of chicken meat quality

2003 ◽  
Vol 82 (8) ◽  
pp. 1343-1351 ◽  
Author(s):  
JF Young ◽  
J Stagsted ◽  
SK Jensen ◽  
AH Karlsson ◽  
P Henckel
Keyword(s):  
Ascorbic Acid ◽  
Meat Quality ◽  
Chicken Meat ◽  
Alpha Tocopherol

scholarly journals Implications for Degenerative Disorders: Antioxidative Activity, Total Phenols, Flavonoids, Ascorbic Acid, β-Carotene and β-Tocopherol inAloe vera

2009 ◽  
Vol 2 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Nurten Ozsoy ◽  
Eda Candoken ◽  
Nuriye Akev
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Biological Effects ◽  
Aloe Vera ◽  
Total Phenols ◽  
Beneficial Effects ◽  
Degenerative Disorders ◽  
Phosphatidylcholine Liposome ◽  
Aqueous Leaf Extract ◽  
Β Carotene

In order to demonstrate whether the known biological effects ofAloe vera(L.) Burm. fil. could correlate with the antioxidant activity of the plant, the antioxidant activity of the aqueous leaf extract was investigated. The present study demonstrated that the aqueous extract fromA. veraleaves contained naturally occuring antioxidant components, including total phenols, flavonoids, ascorbic acid, β-carotene and α-tocopherol. The extract exhibited inhibitory capacity against Fe3+/ascorbic acid induced phosphatidylcholine liposome oxidation, scavenged stable DPPH•, ABTS•+and superoxide anion radicals, and acted as reductant. In contrast, the leaf inner gel did not show any antioxidant activity. It was concluded that the known beneficial effects ofAloe veracould be attributed to its antioxidant activity and could be related to the presence of phenolic compounds and antioxidant vitamins.

scholarly journals Additive effect of alpha-tocopherol and ascorbic acid in combating ethanol-induced hepatic fibrosis

Redox Report ◽  
2013 ◽  
Vol 18 (1) ◽  
pp. 36-46 ◽  
Author(s):  
P Prathibha ◽  
S Rejitha ◽  
R Harikrishnan ◽  
S Syam Das ◽  
P A Abhilash ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Hepatic Fibrosis ◽  
Additive Effect ◽  
Alpha Tocopherol

scholarly journals EFFECTS OF L-ASCORBIC ACID AND ALPHA-TOCOPHEROL ON BIOCHEMICAL PARAMETERS OF SWIMMING-INDUCED OXIDATIVE STRESS IN SERUM OF GUINEA PIGS

2016 ◽  
Vol 13 (4) ◽  
pp. 29-33 ◽  
Author(s):  
Marija Bursać-Mitrović ◽  
Dragan R. Milovanović ◽  
Radoslav Mitić ◽  
Danijela Jovanović ◽  
Miroslav Sovrlić ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Guinea Pigs ◽  
Biochemical Parameters ◽  
Alpha Tocopherol

scholarly journals Antigoitrogenic effect of combined supplementation with dl-alpha-tocopherol, ascorbic acid and beta-carotene and of dl-alpha-tocopherol alone in the rat

1998 ◽  
Vol 156 (3) ◽  
pp. 551-561 ◽  
Author(s):  
JF Mutaku ◽  
MC Many ◽  
I Colin ◽  
JF Denef ◽  
MF van den Hove
Keyword(s):  
Endothelial Cells ◽  
Ascorbic Acid ◽  
Vitamin E ◽  
Beta Carotene ◽  
Iodine Content ◽  
Fold Increase ◽  
Alpha Tocopherol ◽  
Free Radical Scavengers ◽  
Rate Of Increase ◽  
Male Wistar Rats

The effects of the vitamins dl-alpha-tocopherol, ascorbic acid and beta-carotene, free radical scavengers and lipid peroxidation inhibitors, were analyzed in male Wistar rats made goitrous by feeding a low iodine diet (< 20 micrograms iodine/kg) and perchlorate (1% in drinking water) for 4, 8, 16, and 32 days. Groups of control or goitrous rats received for at least 16 days before killing a diet containing 0.6% vitamin E (as dl-alpha-tocopherol acetate), 1.2% vitamin C (ascorbic acid) and 0.48% beta-carotene, either simultaneously (vitamin cocktail) or separately. This treatment led to a 5-fold increase of vitamin E in the thyroid gland, a 24-fold increase in the liver and a 3-fold increase in the plasma. In control rats, vitamin cocktail administration increased slightly the thyroid weight with little changes in thyroid function parameters. During iodine deficiency, administration of the vitamin cocktail or vitamin E alone reduced significantly the rate of increase in thyroid weight, and DNA and protein contents, as well as the proportion of [3H]thymidine labeled thyroid follicular cells, but not that of labeled endothelial cells. Plasma tri-iodothyronine, thyroxine, TSH levels, thyroid iodine content and concentration as well as relative volumes of glandular compartments were not modified. The proportion of necrotic cells rose from 0.5% in normal animals to about 2% after 16 days of goiter development. No significant protective effect of the vitamins was observed. These results suggest that these vitamins, particularly vitamin E, modulate one of the regulatory cascades involved in the control of thyroid follicular cell growth, without interfering with the proliferation of endothelial cells.

scholarly journals Mutator Foci Are Regulated by Developmental Stage, RNA, and the Germline Cell Cycle in Caenorhabditis elegans

2020 ◽  
Vol 10 (10) ◽  
pp. 3719-3728 ◽  
Author(s):  
Celja J. Uebel ◽  
Dana Agbede ◽  
Dylan C. Wallis ◽  
Carolyn M. Phillips
Keyword(s):  
Cell Cycle ◽  
Caenorhabditis Elegans ◽  
Epigenetic Inheritance ◽  
Germline Cell ◽  
Late Pachytene ◽  
Larval Stages ◽  
Transcriptional Inhibition ◽  
Male Germline ◽  
Early Embryos ◽  
Adult Hermaphrodite

RNA interference is a crucial gene regulatory mechanism in Caenorhabditis elegans. Phase-separated perinuclear germline compartments called Mutator foci are a key element of RNAi, ensuring robust gene silencing and transgenerational epigenetic inheritance. Despite their importance, Mutator foci regulation is not well understood, and observations of Mutator foci have been largely limited to adult hermaphrodite germlines. Here we reveal that punctate Mutator foci arise in the progenitor germ cells of early embryos and persist throughout all larval stages. They are additionally present throughout the male germline and in the cytoplasm of post-meiotic spermatids, suggestive of a role in paternal epigenetic inheritance. In the adult germline, transcriptional inhibition results in a pachytene-specific loss of Mutator foci, indicating that Mutator foci are partially reliant on RNA for their stability. Finally, we demonstrate that Mutator foci intensity is modulated by the stage of the germline cell cycle and specifically, that Mutator foci are brightest and most robust in the mitotic cells, transition zone, and late pachytene of adult germlines. Thus, our data defines several new factors that modulate Mutator foci morphology which may ultimately have implications for efficacy of RNAi in certain cell stages or environments.

scholarly journals Isolation, characterization and epistasis of fluoride-resistant mutants of Caenorhabditis elegans.

Genetics ◽  
1994 ◽  
Vol 136 (1) ◽  
pp. 145-154
Author(s):  
I Katsura ◽  
K Kondo ◽  
T Amano ◽  
T Ishihara ◽  
M Kawakami
Keyword(s):  
Caenorhabditis Elegans ◽  
Brood Size ◽  
Normal Growth ◽  
Fluoride Ion ◽  
Wild Type ◽  
Signal Transduction System ◽  
Nematode Caenorhabditis Elegans ◽  
C Elegans ◽  
Class 1 ◽  
Resistant Mutants

Abstract We have isolated 13 fluoride-resistant mutants of the nematode Caenorhabditis elegans. All the mutations are recessive and mapped to five genes. Mutants in three of the genes (class 1 genes: flr-1 X, flr-3 IV, and flr-4 X) are resistant to 400 micrograms/ml NaF. Furthermore, they grow twice as slowly as and have smaller brood size than wild-type worms even in the absence of fluoride ion. In contrast, mutants in the other two genes (class 2 genes: flr-2 V and flr-5 V) are only partially resistant to 400 micrograms/ml NaF, and they have almost normal growth rates and brood sizes in the absence of fluoride ion. Studies on the phenotypes of double mutants showed that class 2 mutations are epistatic to class 1 mutations concerning growth rate and brood size but hypostatic with respect to fluoride resistance. We propose two models that can explain the epistasis. Since fluoride ion depletes calcium ion, inhibits some protein phosphatases and activates trimeric G-proteins, studies on these mutants may lead to discovery of a new signal transduction system that controls the growth of C. elegans.

scholarly journals The Caenorhabditis elegans TDRD5/7-like protein, LOTR-1, interacts with the helicase ZNFX-1 to balance epigenetic signals in the germline

2021 ◽  
Author(s):  
Elisabeth A Marnik ◽  
Miguel Vasconcelos Almeida ◽  
P Giselle Cipriani ◽  
George Chung ◽  
Edoardo Caspani ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Small Rna ◽  
Small Rnas ◽  
Brood Size ◽  
Homologous Proteins ◽  
P Granules ◽  
Transposon Silencing ◽  
Tudor Domain ◽  
Germ Granules

LOTUS and Tudor domain containing proteins have critical roles in the germline. Proteins that contain these domains, such as Tejas/Tapas in Drosophila, help localize Vasa to the germ granules and facilitate piRNA-mediated transposon silencing. The homologous proteins in mammals, TDRD5 and TDRD7, are required during spermiogenesis. Until now, proteins containing both LOTUS and Tudor domains in Caenorhabditis elegans have remained elusive. Here we describe LOTR-1 (D1081.7), which derives its name from its LOTUS and Tudor domains. Interestingly, LOTR-1 docks next to P granules to colocalize with the broadly conserved Z-granule helicase, ZNFX-1. LOTR-1's Z-granule association requires its Tudor domain, but both LOTUS and Tudor deletions affect brood size when coupled with a knockdown of the Vasa homolog glh-1. In addition to interacting with the germ-granule components WAGO-1, PRG-1 and DEPS-1, we identified a Tudor-dependent association with ZNFX-1. Like znfx-1 mutants, lotr-1 mutants lose small RNAs from the 3' ends of WAGO and Mutator targets, reminiscent of the loss of piRNAs from the 3' ends of piRNA precursor transcripts in mouse Tdrd5 mutants. Our work suggests that LOTR-1 acts in a conserved mechanism that brings small RNA generating mechanisms towards the 3' ends of small RNA templates or precursors.

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