Autoimmune thyroid patients with CTLA-4 (+49A/G) GG/AG genotypes have high seropositivity to thyroid peroxidase than thyroglobulin

Meta Gene ◽  
2022 ◽  
pp. 101010
Author(s):  
Nusrath Fathima ◽  
Qursheed Sultana ◽  
Syyeda Anees ◽  
Kaleem Ullah ◽  
Vitaly Ryu ◽  
...  
Keyword(s):  
Thyroid Peroxidase ◽  
Autoimmune Thyroid

scholarly journals Purification of the human thyroid peroxidase and its identification as the microsomal antigen involved in autoimmune thyroid diseases

FEBS Letters ◽  
1985 ◽  
Vol 190 (1) ◽  
pp. 147-152 ◽  
Author(s):  
Barbara Czarnocka ◽  
Jean Ruf ◽  
Mireille Ferrand ◽  
Pierre Carayon ◽  
Serge Lissitzky
Keyword(s):  
Thyroid Diseases ◽  
Human Thyroid ◽  
Thyroid Peroxidase ◽  
Autoimmune Thyroid Diseases ◽  
Autoimmune Thyroid ◽  
Human Thyroid Peroxidase ◽  
Microsomal Antigen

scholarly journals Seasonality of month of birth of patients with Graves’ and Hashimoto’s diseases differ from that in the general population

2007 ◽  
Vol 156 (6) ◽  
pp. 631-636 ◽  
Author(s):  
Gerasimos E Krassas ◽  
Konstantinos Tziomalos ◽  
Nikolaos Pontikides ◽  
Hadas Lewy ◽  
Zvi Laron
Keyword(s):  
General Population ◽  
Perinatal Period ◽  
Thyroid Peroxidase ◽  
Month Of Birth ◽  
Autoimmune Thyroid ◽  
Common Etiology ◽  
Antibody Titers ◽  
Low Levels ◽  
Antibody Levels

Objective: We aimed to test the viral hypothesis in the pathogenesis of autoimmune thyroid disease (AITD). Design: We determined the pattern of month of birth (MOB) distribution in patients with AITD and in the general population and searched for differences between them. Methods: A total of 1023 patients were included in this study; 359 patients had Graves’ hyperthyroidism (GrH) and 664 had Hashimoto’s hypothyroidism (HH). We divided the patients with HH into three subgroups according to their thyroid peroxidase (TPO) antibody titers at diagnosis: low levels (<500 IU/ml), high levels (500–1000 IU/ml), and extremely high levels (>1000 IU/ml). We used cosinor analysis to analyze the data. Results: Overall, patients with GrH and HH had a different pattern of MOB distribution when compared with the general population and between groups. Furthermore, among both patients with GrH and HH, both genders had a different pattern of MOB distribution when compared with the general population and this pattern was also different between genders. Finally, only women with extremely high titers of TPO antibodies at diagnosis and men with low or extremely high TPO antibody levels showed rhythmicity in MOB, with a pattern of MOB distribution different from that in controls. Conclusions: The different MOB seasonality in both GrH and HH points towards a similar maybe even common etiology with type 1 diabetes mellitus and multiple sclerosis, namely a seasonal viral infection as the initial trigger in the perinatal period, the clinical disease resulting from further specific damage over time.

Enzymatic deglycosylation of porcine thyroid peroxidase: effects on catalytic activity and immunoreactivity

1991 ◽  
Vol 124 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Egberto G. Moura ◽  
Carmen C. Pazos-Moura ◽  
Naokata Yokoyama ◽  
Martha L. Dorris ◽  
Alvin Taurog
Keyword(s):  
Catalytic Activity ◽  
Molecular Mass ◽  
Antigenic Determinants ◽  
Enzyme Linked Immunosorbent Assay ◽  
Minor Role ◽  
Relative Molecular Mass ◽  
Thyroid Peroxidase ◽  
Autoimmune Thyroid ◽  
Tryptic Fragment ◽  
A Minor

Abstract Thyroid peroxidase is a heme-containing, membrane-bound, glycoprotein enzyme that catalyzes iodination and coupling in the thyroid gland. It is also the antigen for microsomal autoantibodies that are commonly found in the serum of patients with autoimmune thyroid disease. We examined the effect of deglycosylation on the catalytic functions and the immunoreactivity of this enzyme. A highly purified, solubilized, large tryptic fragment of porcine thyroid peroxidase, retaining all of the N-linked glycosylation sites of the native enzyme and displaying full catalytic activity was used. It was deglycosylated by treatment with N-glycanase under nondenaturing conditions. The loss in relative molecular mass after treatment, determined by gel electrophoresis, was about 75% of the estimated molecular weight of the glycan portion of porcine thyroid peroxidase. Lectin blots performed with horseradish peroxidase-conjugated concanavalin A showed a similar loss in relative molecular mass but some residual carbohydrate. The intensity of the carbohydrate stain was consistent with the loss of about 75% of the glycans. Despite this loss, three different assays for catalytic activity of porcine thyroid peroxidase were not significantly decreased. Immunoreactivity measured by immunoblotting and by enzyme-linked immunosorbent assay was also unimpaired. These findings suggest that N-glycanase-sensitive glycans in porcine thyroid peroxidase do not act as antigenic determinants and play a minor role, if any, in catalytic activity and, presumably therefore, in the maintenance of protein conformation.

Expression of Recombinant Human Thyroid Peroxidase by the Baculovirus System and Its Use in Elisa Screening for Diagnosis of Autoimmune Thyroid Disease

Autoimmunity ◽  
1993 ◽  
Vol 15 (4) ◽  
pp. 275-284 ◽  
Author(s):  
Heinz Haubruck ◽  
Ludwig Mauch ◽  
Neil J. Cook ◽  
Ute Steffens ◽  
Nicholas Hunt ◽  
...  
Keyword(s):  
Thyroid Disease ◽  
Autoimmune Thyroid Disease ◽  
Human Thyroid ◽  
Thyroid Peroxidase ◽  
Autoimmune Thyroid ◽  
Human Thyroid Peroxidase ◽  
Baculovirus System

Autoimmune thyroid disease in diabetic children and adolescents: a single center study from south Punjab

2021 ◽  
Vol 71 (7) ◽  
pp. 1804-1807
Author(s):  
Waqas Imran Khan ◽  
Erum Afzal ◽  
Sajjad Hussain
Keyword(s):  
Children And Adolescents ◽  
Thyroid Disease ◽  
Autoimmune Thyroid Disease ◽  
Glycosylated Hemoglobin ◽  
Overt Hypothyroidism ◽  
Thyroid Peroxidase ◽  
Pediatric Endocrinology ◽  
Autoimmune Thyroid ◽  
Thyroid Peroxidase Antibody ◽  
Diabetic Children

Objective: To evaluate the prevalence of autoimmune thyroid disease (AITD) in diabetic children in south Punjab. Methods: This was an observational cross sectional study from Jan 2019 to Dec 2019 in the outpatient diabetic clinic of the department of pediatric endocrinology at Children Hospital and The Institute of Child Health Multan. A total of 161 consecutive patients of both genders with TIDM were enrolled in this study after taking informed consent. Blood samples for Thyroid functions testes including thyroid stimulating hormone (TSH), free thyroxin (fT4), Thyroid peroxidase antibody (TPO-Ab), thyroglobulin antibody (TG-Ab) and glycosylated hemoglobin (HbA1C) level were sent. Results: Among diabetic children males were 83 (51.6%). Age range was 2-15 years. Mean age and standard deviation was 9.7± 4.3. TPO-Ab was positive in 34 patients (21.1%) and TG-Ab in 27 patients (16.7%), whereas both antibodies were positive in 17 patients (10.5%). Six patients (3.7%) had evidence of subclinical hypothyroidism, 8 patients (4.9%) had overt hypothyroidism and 1 patient (0.62%) had hyperthyroidism Conclusion: The prevalence of AITD among children and adolescents with type 1 diabetes mellitus was 21.1% in our study. Hypothyroidism was more prevalent in these children compared to hyperthyroidism. All diabetic children should be screened for AITD. Thyroid functions should be checked where TPO antibody is positive. Keywords: Autoimmune thyroid disease, anti thyroid peroxidase antibody, anti thyroglobulin Continuous...

scholarly journals Thyroid Peroxidase Revisited – Whatʼs New?

2019 ◽  
Vol 51 (12) ◽  
pp. 765-769 ◽  
Author(s):  
Marlena Godlewska ◽  
Damian Gawel ◽  
Ashley M. Buckle ◽  
J. Paul Banga
Keyword(s):  
Thyroid Hormone ◽  
Environmental Factors ◽  
Recent Progress ◽  
Thyroid Peroxidase ◽  
Autoimmune Thyroid Diseases ◽  
Autoimmune Thyroid ◽  
Thyroid Hormone Biosynthesis ◽  
Hormone Biosynthesis ◽  
Genetic And Environmental Factors ◽  
Thyroid Dysfunctions

AbstractThyroid peroxidase (TPO) is an enzyme that participates in thyroid hormone biosynthesis. TPO is also a major autoantigen in autoimmune thyroid diseases (AITD). In this review, we summarize the latest developments in the field of TPO research. We present the current understanding of immunodominant serologic determinants, frequency of TPO-specific autoantibodies in the population, as well as genetic and environmental factors contributing to their development. Moreover, we report recent progress in the clinical utilities of TPO autoantibody testing, including thyroid dysfunctions and extra-thyroidal disorders.

The human anti-thyroid peroxidase autoantibody repertoire in Graves' and Hashimoto's autoimmune thyroid diseases

2002 ◽  
Vol 54 (3) ◽  
pp. 141-157 ◽  
Author(s):  
Thierry Chardès ◽  
Nicolas Chapal ◽  
Damien Bresson ◽  
Cédric Bès ◽  
Véronique Giudicelli ◽  
...  
Keyword(s):  
Thyroid Diseases ◽  
Thyroid Peroxidase ◽  
Autoimmune Thyroid Diseases ◽  
Autoimmune Thyroid

THYROID ANTIBODIES LEVELS IN EUTHYROID MEN AND WOMEN - RESIDENTS OF THE ARCTIC ZONE OF THE RUSSIAN FEDERATION

2019 ◽  
Vol 64 (9) ◽  
pp. 541-545
Author(s):  
I. N. Gorenko
Keyword(s):  
Russian Federation ◽  
Clinical Importance ◽  
Thyroid Function Test ◽  
The Arctic ◽  
Thyroid Peroxidase ◽  
Thyroid Antibodies ◽  
Arctic Zone ◽  
Men And Women ◽  
Autoimmune Thyroid ◽  
The Russian Federation

The aim of the study was to determine the levels of thyroid antibodies and their relationship with thyroid hormones and thyroglobulin in euthyroid men and women, residents of the Arctic zone of the Russian Federation. A total of 208 apparently healthy people were enrolled in this study and classified into two groups depending on gender and level of autoantibodies in the blood. Serum hormones of the pituitary-thyroid system, thyroglobulin and antibodies concentration was measured by enzyme immunoassay. The prevalence of positive antibodies among various examined groups was determined and the characteristics of euthyroid subjects with abnormal thyroid antibodies levels were studied. Circulating positive thyroid antibodies were found in 20% of the surveyed residents of the Arctic zone of the Russian Federation. The median serum antibodies against thyroid peroxidase (AntiTPO) or thyroglobulin (AntiTG) levels and the percentage of people in general population with positive antibodies (i.e. AntiTPO ≥ 50 IU / ml and / or AntiTG ≥ 100 IU / ml) were statistically significant higher in women than in men, p < 0.01. Such thyroid antibodies levels were associated with a higher thyroid gland functional activity in women, which was demonstrated by significantly higher thyroxin level and lower thyroglobulin value in the peripheral blood. Part of women positive for AntiTPO increased with age from 18 to 33% (in groups aged 18-44 and 45-59 years, p = 0.04). The findings of the study reveal correlation between thyroid function test and thyroid antibodies levels, elaborating the clinical importance of thyroid antibodies in clinical examination and follow-up of patients with autoimmune thyroid disorders.

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