scholarly journals Full-genome sequences of porcine circovirus 3 (PCV3) and high prevalence in mummified fetuses from commercial farms in Brazil

2020 ◽  
Vol 141 ◽  
pp. 104027 ◽  
Author(s):  
Ana Carolina Dal Santo ◽  
Kamila C. Cezario ◽  
Paulo E. Bennemann ◽  
Sergio A. Machado ◽  
Mathias Martins
Keyword(s):  
Porcine Circovirus ◽  
Full Genome ◽  
Genome Sequences ◽  
High Prevalence ◽  
Commercial Farms ◽  
Porcine Circovirus 3

scholarly journals Full-genome sequencing of porcine circovirus 3 field strains from Denmark, Italy and Spain demonstrates a high within-Europe genetic heterogeneity

2018 ◽  
Vol 65 (3) ◽  
pp. 602-606 ◽  
Author(s):  
G. Franzo ◽  
M. Legnardi ◽  
C. K. Hjulsager ◽  
F. Klaumann ◽  
L. E. Larsen ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Genetic Heterogeneity ◽  
Porcine Circovirus ◽  
Full Genome ◽  
Full Genome Sequencing ◽  
Porcine Circovirus 3

scholarly journals Viral metagenomics analysis demonstrates the diversity of viral flora in piglet diarrhoeic faeces in China

2014 ◽  
Vol 95 (7) ◽  
pp. 1603-1611 ◽  
Author(s):  
Bin Zhang ◽  
Cheng Tang ◽  
Hua Yue ◽  
Yupeng Ren ◽  
Zhigang Song
Keyword(s):  
Prevalence Rate ◽  
Viral Metagenomics ◽  
Porcine Circovirus ◽  
Diarrhea Virus ◽  
Porcine Enterovirus ◽  
Faecal Samples ◽  
High Prevalence ◽  
Commercial Farms ◽  
Viral Communities ◽  
First Time

To investigate the diversity of viral flora, we used metagenomics to study the viral communities in a pooled faecal sample of 27 diarrhoeic piglets from intensive commercial farms in China. The 15 distinct mammalian viruses identified in the pooled diarrhoeic sample were, in order of abundance of nucleic acid sequence, Porcine epidemic diarrhea virus (PEDV), sapovirus, porcine bocavirus-4 (PBoV-4), sapelovirus, torovirus, coronavirus, PBoV-2, stool-associated single-stranded DNA virus (poSCV), astrovirus (AstV), kobuvirus, posavirus-1, porcine enterovirus-9 (PEV-9), porcine circovirus-like (po-circo-like) virus, picobirnavirus (PBV) and Torque teno sus virus 2 (TTSuV-2). The prevalence rate of each virus was verified from diarrhoeic and healthy piglets by PCR assay. A mean of 5.5 different viruses were shed in diarrhoeic piglets, and one piglet was in fact co-infected with 11 different viruses. By contrast, healthy piglets shed a mean of 3.2 different viruses. Compared with samples from healthy piglets, the co-infection of PEDV and PBoV had a high prevalence rate in diarrhoea samples, suggesting a correlation with the appearance of diarrhoea in piglets. Furthermore, we report here for the first time the presence of several recently described viruses in China, and the identification of novel genotypes. Therefore, our investigation results provide an unbiased survey of viral communities and prevalence in faecal samples of piglets.

scholarly journals Full-Length Genome Sequences of Two Chinese Porcine Circovirus Type 3 Strains, NWHEB21 and NWHUN2

2018 ◽  
Vol 6 (7) ◽  
Author(s):  
Can Liu ◽  
Shasha Chen ◽  
Fanwei Meng ◽  
Rui Chen ◽  
Zhigang Zhang ◽  
...  
Keyword(s):  
Complete Genome ◽  
Porcine Circovirus Type ◽  
Full Length ◽  
Porcine Circovirus ◽  
Genome Sequences ◽  
Porcine Circovirus 3 ◽  
Type 3 ◽  
Full Length Genome ◽  
Brain Tissues

ABSTRACT Two porcine circovirus 3 (PCV3) strains, named NWHEB21 and NWHUN2, were identified in heart and brain tissues of aborted piglets. Their complete genome sequences were sequenced and analyzed to further characterize PCV3 in China and worldwide.

Molecular Characterization of Novel Porcine Circovirus 3 (PCV3) in Pig Populations in the North of Vietnam

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Nguyen Van Giap ◽  
Chung Hee Chun ◽  
Huynh Thi My Le ◽  
Cao Thi Bich Phuong ◽  
Vu Thi Ngoc ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Porcine Circovirus ◽  
The North ◽  
Porcine Circovirus 3

scholarly journals Porcine Gammaherpesviruses in Italian Commercial Swine Population: Frequent but Harmless

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 47
Author(s):  
Giovanni Franzo ◽  
Michele Drigo ◽  
Matteo Legnardi ◽  
Laura Grassi ◽  
Maria Luisa Menandro ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Clinical Signs ◽  
Northern Italy ◽  
Porcine Circovirus ◽  
Swine Industry ◽  
Positive Interaction ◽  
High Prevalence ◽  
Swine Population ◽  
Clinical Conditions

Differently from alpha- and betaherpesviruses affecting swine, interest in the recently discovered Suid gammaherpesvirus 3, Suid gammaherpesvirus 4, and Suid gammaherpesvirus 5, also known as porcine lymphotropic herpesviruses (PLHV-1, PLHV-2, and PLHV-3), has largely focused on their role as potential zoonotic agents in cases of xenotransplantation. However, their role as primary pathogens of swine or as co-factors for other lymphotropic infections has essentially been neglected. The present study aims at filling this gap, evaluating the association between PLHVs infection and different clinical conditions and/or porcine circovirus (PCV) co-infection. One hundred seventy-six samples were obtained from different animals located in a high-density pig area of Northern Italy in the period 2017–2020. The presence of PLHVs and PCVs was tested and quantified by specific real-time PCR: PLHVs were widespread among pigs (PLHV-1, PLHV-2, and PLHV-3 prevalence was 28.97%, 10.79%, and 4.54%, respectively) and detected in all considered tissues and clinical conditions. Frequent co-infections were also observed among PLHVs and with PCVs, although a significant association was not detected with the exception of a positive interaction between PLHV-1 and PLHV-3, and a negative one between PLHV-2 and PCV-2. Significantly, no association between PLHVs, alone or in co-infection, emerged with any of the considered clinical signs, their frequency being comparable between healthy and diseased animals. Based on these pieces of evidence and despite their high prevalence, PLHVs’ relevance for the swine industry appears negligible, either as primary pathogens or as predisposing factors for circovirus-induced diseases.

Enzymatic recombinase amplification coupled with CRISPR-Cas12a for ultrasensitive, rapid, and specific Porcine circovirus 3 detection

2021 ◽  
Vol 59 ◽  
pp. 101763
Author(s):  
Wuyin Zhang ◽  
Liang Xu ◽  
Qi Liu ◽  
Yingli Cao ◽  
Kankan Yang ◽  
...  
Keyword(s):  
Porcine Circovirus ◽  
Porcine Circovirus 3

scholarly journals Genetic and Antigenic Characterization of Avian Avulavirus Type 6 (AAvV-6) Circulating in Canadian Wild Birds (2005–2017)

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 543
Author(s):  
Tamiko Hisanaga ◽  
Catherine Soos ◽  
Nicola Lewis ◽  
Oliver Lung ◽  
Matthew Suderman ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Fusion Gene ◽  
Full Genome ◽  
Genome Sequences ◽  
Viral Shedding ◽  
Genetic Groups ◽  
Antigenic Characterization ◽  
Specific Pathogen ◽  
First Time

We describe for the first time the genetic and antigenic characterization of 18 avian avulavirus type-6 viruses (AAvV-6) that were isolated from wild waterfowl in the Americas over the span of 12 years. Only one of the AAvV-6 viruses isolated failed to hemagglutinate chicken red blood cells. We were able to obtain full genome sequences of 16 and 2 fusion gene sequences from the remaining 2 isolates. This is more than double the number of full genome sequences available at the NCBI database. These AAvV-6 viruses phylogenetically grouped into the 2 existing AAvV-6 genotype subgroups indicating the existence of an intercontinental epidemiological link with other AAvV-6 viruses isolated from migratory waterfowl from different Eurasian countries. Antigenic maps made using HI assay data for these isolates showed that the two genetic groups were also antigenically distinct. An isolate representing each genotype was inoculated in specific pathogen free (SPF) chickens, however, no clinical symptoms were observed. A duplex fusion gene based real-time assay for the detection and genotyping of AAvV-6 to genotype 1 and 2 was developed. Using the developed assay, the viral shedding pattern in the infected chickens was examined. The chickens infected with both genotypes were able to shed the virus orally for about a week, however, no significant cloacal shedding was detected in chickens of both groups. Chickens in both groups developed detectable levels of anti-hemagglutinin antibodies 7 days after infection.

scholarly journals Full-Genome Sequences of Influenza A(H1N1)pdm09 Viruses Isolated from Finnish Patients from 2009 to 2013

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
T. Lakspere ◽  
J. Tynell ◽  
M. Kaloinen ◽  
M. Vanlede ◽  
A. Parsons ◽  
...  
Keyword(s):  
Influenza A ◽  
Full Genome ◽  
Genome Sequences ◽  
Influenza A H1n1

Loop-mediated isothermal amplification assay for the rapid and visual detection of novel porcine circovirus 3

2018 ◽  
Vol 253 ◽  
pp. 26-30 ◽  
Author(s):  
Yu-Ri Park ◽  
Hye-Ryung Kim ◽  
Seong-Hee Kim ◽  
Kyoung-Ki Lee ◽  
Young S. Lyoo ◽  
...  
Keyword(s):  
Visual Detection ◽  
Isothermal Amplification ◽  
Porcine Circovirus ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay ◽  
Porcine Circovirus 3

scholarly journals Molecular detection and clinical aspects of porcine circovirus 3 infection in pigs from Brazil

2020 ◽  
Vol 72 (5) ◽  
pp. 1731-1736
Author(s):  
A.C.M. Cruz ◽  
I.L.F. Rodrigues ◽  
A.E. Souza ◽  
F.B. Knackfuss ◽  
R.L. Silveira ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Porcine Circovirus ◽  
Southeastern Brazil ◽  
Clinical Aspects ◽  
Statistical Association ◽  
Serum Samples ◽  
Clinical Presentations ◽  
Porcine Circovirus 3 ◽  
Clinical Conditions ◽  
Swine Serum

ABSTRACT Porcine circovirus 3 (PCV-3) DNA has been detected in serum samples from apparently healthy pigs as well as pigs with different clinical conditions. Molecular detection of PCV-3 was observed in swine serum samples from Southeastern - Brazil using a nested PCR designed specifically for this study. The epidemiology and clinical aspects of PCV-3 infection were evaluated. The samples originated from 154 pigs of both genders from different production phases and with different clinical presentations, sampled from 31 pig farms visited between 2013 and 2018. In this study, PCV-3 was detected in 26.7% of samples from all populations across varying ages. Statistical association (P=0.0285) was observed only between animals with respiratory signs and PCV-3; no PCV-3-positive animal had diarrhea. No statistical association was observed between PCV-3 and age, or gender of the pigs. Because PCV-3 is a newly discovered virus, there is very little information about its epidemiology. We hope that these data can help in future studies investigating PCV-3 epidemiology.

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