In vitro immune responses of bovine mammary epithelial cells induced by Escherichia coli, with multidrug resistant extended-spectrum β-lactamase, isolated from mastitic milk

2020 ◽  
Vol 149 ◽  
pp. 104494
Author(s):  
Cuicui Zhuang ◽  
Wenlin Huo ◽  
Gang Liu ◽  
Muhammad Shahid ◽  
Jian Gao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Immune Responses ◽  
Mammary Epithelial Cells ◽  
Multidrug Resistant ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Extended Spectrum ◽  
Mastitic Milk

Exogenous phospholipase A2 affects inflammatory gene expression in primary bovine mammary epithelial cells

2019 ◽  
Vol 86 (2) ◽  
pp. 177-180
Author(s):  
Jacqueline P. Kurz ◽  
Mark P. Richards ◽  
Matthew Garcia ◽  
Zhongde Wang
Keyword(s):  
Epithelial Cells ◽  
Phospholipase A2 ◽  
Inflammatory Responses ◽  
Mammary Epithelial Cells ◽  
Constitutive Expression ◽  
Mammary Epithelial ◽  
Inflammatory Factors ◽  
Bovine Mammary Epithelial Cells ◽  
Lps Challenge

AbstractThis Research Communication addresses the hypothesis that exogenously administered phospholipase A2 (PLA2) affects the inflammatory responses of bovine mammary epithelial cells (bMEC) in vitro with the aim of providing preliminary justification of investigation into the uses of exogenously administered PLA2 to manage or treat bovine mastitis. Primary bMEC lines from 11 lactating Holstein dairy cows were established and the expression of 14 pro-inflammatory genes compared under unchallenged and lipopolysaccharide (LPS)-challenged conditions, with and without concurrent treatment with bovine pancreatic PLA2G1B, a secreted form of PLA2. No differences in the expression of these genes were noted between PLA2-treated and untreated bMEC under unchallenged conditions. Following LPS challenge, untreated bMEC exhibited significant downregulation of CXCL8, IL1B, CCL20, and CXCL1. In contrast, PLA2-treated bMEC exhibited significant downregulation of IL1B and CCL20 only. These findings indicate that exogenous PLA2 affects the expression of some pro-inflammatory factors in immune-stimulated bMEC, but does not influence the constitutive expression of these factors. Further investigation of the influence of exogenous PLA2 in the bovine mammary gland is justified.

scholarly journals MicroRNA expression profiling of porcine mammary epithelial cells after challenge with Escherichia coli in vitro

BMC Genomics ◽  
2017 ◽  
Vol 18 (1) ◽  
Author(s):  
A. Jaeger ◽  
F. Hadlich ◽  
N. Kemper ◽  
A. Lübke-Becker ◽  
E. Muráni ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Expression Profiling ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Microrna Expression
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