Assessment of H2O2/albumin and glucose on the biomedical iron alloys corrosion in simulated body fluid: experimental, surface, and computational investigations

A modified one-step process was used to prepare tetracalcium phosphate/monetite/calcium sulfate hemihydrate powder cement mixtures (CAS). The procedure allowed the formation of monetite and calcium sulfate hemihydrate (CSH) in the form of nanoparticles. It was hypothesized that the presence of nanoCSH in small amounts enhances the in vitro bioactivity of CAS cement in relation to osteogenic gene markers in mesenchymal stem cells (MSCs). The CAS powder mixtures with 15 and 5 wt.% CSH were prepared by milling powder tetracalcium phosphate in an ethanolic solution of both orthophosphoric and sulfuric acids. The CAS cements had short setting times (around 5 min). The fast setting of the cement samples after the addition of the liquid component (water solution of NaH2PO4) was due to the partial formation of calcium sulfate dihydrate and hydroxyapatite before soaking in SBF with a small change in the original phase composition in cement powder samples after milling. Nanocrystalline hydroxyapatite biocement was produced by soaking of cement samples after setting in simulated body fluid (SBF). The fast release of calcium ions from CAS5 cement, as well as a small rise in the pH of SBF during soaking, were demonstrated. After soaking in SBF for 7 days, the final product of the cement transformation was nanocrystalline hydroxyapatite. The compressive strength of the cement samples (up to 30 MPa) after soaking in simulated body fluid (SBF) was comparable to that of bone. Real time polymerase chain reaction (RT-PCR) analysis revealed statistically significant higher gene expressions of alkaline phosphatase (ALP), osteonectin (ON) and osteopontin (OP) in cells cultured for 14 days in CAS5 extract compared to CSH-free cement. The addition of a small amount of nanoCSH (5 wt.%) to the tetracalcium phosphate (TTCP)/monetite cement mixture significantly promoted the over expression of osteogenic markers in MSCs. The prepared CAS powder mixture with its enhanced bioactivity can be used for bone defect treatment and has good potential for bone healing.

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Surface-Directed Mineralization of Fibrous Collagen Scaffolds in Simulated Body Fluid for Tissue Engineering Applications

ACS Applied Bio Materials ◽

10.1021/acsabm.0c01507 ◽

2021 ◽

Vol 4 (3) ◽

pp. 2514-2522

Author(s):

Odair Bim-Júnior ◽

Fabiana Curylofo-Zotti ◽

Mariana Reis ◽

Yvette Alania ◽

Paulo N. Lisboa-Filho ◽

...

Keyword(s):

Tissue Engineering ◽

Simulated Body Fluid ◽

Body Fluid ◽

Collagen Scaffolds ◽

Engineering Applications

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Preparation and Degradation Characteristics of MAO/APS Composite Bio-Coating in Simulated Body Fluid

Coatings ◽

10.3390/coatings11060667 ◽

2021 ◽

Vol 11 (6) ◽

pp. 667

Author(s):

Zexin Wang ◽

Fei Ye ◽

Liangyu Chen ◽

Weigang Lv ◽

Zhengyi Zhang ◽

...

Keyword(s):

Simulated Body Fluid ◽

Composite Coating ◽

Body Fluid ◽

Composite Coatings ◽

Degradation Process ◽

Ceramic Coatings ◽

Immersion Test ◽

Substrate Material ◽

Atmospheric Plasma

In this work, ZK60 magnesium alloy was employed as a substrate material to produce ceramic coatings, containing Ca and P, by micro-arc oxidation (MAO). Atmospheric plasma spraying (APS) was used to prepare the hydroxyapatite layer (HA) on the MAO coating to obtain a composite coating for better biological activity. The coatings were examined by various means including an X-ray diffractometer, a scanning electron microscope and an energy spectrometer. Meanwhile, an electrochemical examination, immersion test and tensile test were used to evaluate the in vitro performance of the composite coatings. The results showed that the composite coating has a better corrosion resistance. In addition, this work proposed a degradation model of the composite coating in the simulated body fluid immersion test. This model explains the degradation process of the MAO/APS coating in SBF.

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Analysis of corrosion rate at bone implant replacement materials with immersion time variations in simulated body fluid

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/1034/1/012153 ◽

2021 ◽

Vol 1034 (1) ◽

pp. 012153

Author(s):

Atria Pradityana ◽

Nur Husodo ◽

Rizaldy Hakim Ash-Shiddieqy ◽

Muhammad Saiful Rizal

Keyword(s):

Simulated Body Fluid ◽

Corrosion Rate ◽

Body Fluid ◽

Immersion Time ◽

Bone Implant ◽

Time Variations

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Development of Adhesive, Bioactive and Antibacterial Titania Sol-Gel Coating on Titanium Substrate by Dip-Coating Technique

Coatings ◽

10.3390/coatings11020243 ◽

2021 ◽

Vol 11 (2) ◽

pp. 243

Author(s):

Diana Horkavcová ◽

Quentin Doubet ◽

Gisèle Laure Lecomte-Nana ◽

Eva Jablonská ◽

Aleš Helebrant

Keyword(s):

Simulated Body Fluid ◽

Body Fluid ◽

Sol Gel ◽

Titanium Substrate ◽

Dip Coating ◽

Coating Technique ◽

Metal Implants ◽

Excellent Adhesion ◽

Titania Sol ◽

Dip Coating Technique

The sol-gel method provides a wide variety of applications in the medical field. One of these applications is the formation of coatings on the metal implants. The coatings containing specific additive can enhance or improve the existing surface properties of the substrate. In this work, titania sol-gel coatings were doped with two forms of silver (AgNO3, Ag3PO4) and synthetic hydroxyapatite and applied on the titanium samples by dip-coating technique. After drying and slow firing, all coatings were characterized with scanning electron microscopy. Thin coatings were successfully prepared with excellent adhesion to the substrate (measured by ASTM D 3359-2), despite cracks. Coatings containing silver and hydroxyapatite demonstrated a 100% antibacterial effect against Escherichia coli after 24 h. The bioactivity of the coatings containing hydroxyapatite tested in modified simulated body fluid under static-dynamic conditions was confirmed by bone-like hydroxyapatite precipitation. To better understand the interaction of the coatings with simulated body fluid (SBF), changes of Ca2+ and (PO4)3− ions concentrations and pH values were studied.

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