Sulfated polysaccharide isolated from the sea cucumber Stichopus japonicas promotes the SDF-1α/CXCR4 axis-induced NSC migration via the PI3K/Akt/FOXO3a, ERK/MAPK, and NF-κB signaling pathways

Objective: This study aimed to appraise the activity of Pterocladia capillacea and Corallina officinalis polysaccharides against breast cancer stem cells (BCSCs). P. capillacea and C. officinalis polysaccharides were characterized to be sulfated polysaccharide-protein complexes. Methods: Cytotoxicity of the polysaccharides against MDA-MB-231 and MCF-7 cell lines along with their impact on CD44+/CD24− and aldehyde dehydrogenase 1(ALDH1) positive BCSC population were determined. Their effect on gene expression of CSC markers, Wnt/β-catenin and Notch signaling pathways was evaluated. Results: P. capillacea and C. officinalis polysaccharides inhibited the growth of breast cancer cells and reduced BCSC subpopulation. P. capillacea polysaccharides significantly down-regulated OCT4, SOX2, ALDH1A3 and vimentin in MDA-MB-231 as well as in MCF-7 cells except for vimentin that was up-regulated in MCF-7 cells. C. officinalis polysaccharides exhibited similar effects except for OCT4 that was up-regulated in MDA-MB-231 cells. Significant suppression of Cyclin D1 gene expression was noted in MDA-MB-231 and MCF-7 cells treated with P. capillacea or C. officinalis polysaccharides. β-catenin and c-Myc genes were significantly down-regulated in MDA-MB-231 cells treated with C. officinalis and P. capillacea polysaccharides, respectively, while being up-regulated in MCF-7 cells treated with either of them. Additionally, P. capillacea and C. officinalis polysaccharides significantly down-regulated Hes1 gene in MCF-7 cells despite increasing Notch1 gene expression level. However, significant down-regulation of Notch1 gene was observed in MDA-MB-231 cells treated with P. capillacea polysaccharides. Conclusion: Collectively, this study provides evidence for the effectiveness of P. capillacea and C. officinalis polysaccharides in targeting BCSCs through interfering with substantial signaling pathways contributing to their functionality.

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Sulfated polysaccharide of Sepiella Maindroni ink inhibits the migration, invasion and matrix metalloproteinase-2 expression through suppressing EGFR-mediated p38/MAPK and PI3K/Akt/mTOR signaling pathways in SKOV-3 cells

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2017.08.178 ◽

2018 ◽

Vol 107 ◽

pp. 349-362 ◽

Cited By ~ 12

Author(s):

Wenjie Jiang ◽

Yanna Cheng ◽

Na Zhao ◽

Lian Li ◽

Yikang Shi ◽

...

Keyword(s):

Matrix Metalloproteinase ◽

Signaling Pathways ◽

P38 Mapk ◽

Sulfated Polysaccharide ◽

Mtor Signaling ◽

Matrix Metalloproteinase 2 ◽

Sepiella Maindroni

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Oxidative degradation of the sulfated polysaccharide isolated from sea cucumber Holothuria nobilis

Process Biochemistry ◽

10.1016/j.procbio.2014.12.016 ◽

2015 ◽

Vol 50 (2) ◽

pp. 294-301 ◽

Cited By ~ 7

Author(s):

Xiaodi Dong ◽

Rujia Pan ◽

Shanmei Zou ◽

Meilin He ◽

Changhai Wang

Keyword(s):

Sea Cucumber ◽

Oxidative Degradation ◽

Sulfated Polysaccharide

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Inhibition effects of patchouli alcohol against influenza a virus through targeting cellular PI3K/Akt and ERK/MAPK signaling pathways

Virology Journal ◽

10.1186/s12985-019-1266-x ◽

2019 ◽

Vol 16 (1) ◽

Cited By ~ 9

Author(s):

Yunjia Yu ◽

Yang Zhang ◽

Shuyao Wang ◽

Wei Liu ◽

Cui Hao ◽

...

Keyword(s):

Signaling Pathways ◽

Influenza A ◽

Plaque Assay ◽

Mapk Signaling ◽

Western Blot Assay ◽

Patchouli Alcohol ◽

Erk Mapk ◽

Mapk Signaling Pathways

Abstract Background Patchouli alcohol (PA) is a tricyclic sesquiterpene extracted from Pogostemonis Herba, which is a traditional Chinese medicine used for therapy of inflammatory diseases. Recent studies have shown that PA has various pharmacological activities, including anti-bacterial and anti-viral effects. Methods In this study, the anti-influenza virus (IAV) activities and mechanisms were investigated both in vitro and in vivo. The inhibitory effects of PA against IAV in vitro were evaluated by plaque assay and immunofluorescence assay. The neuraminidase inhibition assay, hemagglutination inhibition (HI) assay, and western blot assay were used to explore the anti-viral mechanisms. The anti-IAV activities in vivo were determined by mice pneumonia model and HE staining. Results The results showed that PA significantly inhibited different IAV strains multiplication in vitro, and may block IAV infection through inactivating virus particles directly and interfering with some early stages after virus adsorption. Cellular PI3K/Akt and ERK/MAPK signaling pathways may be involved in the anti-IAV actions of PA. Intranasal administration of PA markedly improved mice survival and attenuated pneumonia symptoms in IAV infected mice, comparable to the effects of Oseltamivir. Conclusions Therefore, Patchouli alcohol has the potential to be developed into a novel anti-IAV agent in the future.

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