‘Helcococcus massiliensis’ sp. nov., a new bacterial species isolated from the vaginal sample of a woman with bacterial vaginosis living in Dielmo, Senegal

A strictly anaerobic, Gram-stain-positive, non motile and non-spore-forming rod-shaped bacterium, strain Marseille-P2666T, was isolated using the culturomics approach from a vaginal sample of a French patient suffering from bacterial vaginosis. Cells were saccharolytic and were negative for catalase, oxidase, urease, nitrate reduction, indole production, hydrolysis of aesculin and gelatin. Strain Marseille-P2666T exhibited 97.04 % 16S rRNA sequence similarity to Collinsella tanakaei type strain YIT 12063T, the phylogenetically closest species with standing in nomenclature. The major fatty acids were C18:1ω9 (38 %), C16 : 0 (24 %) and C18 : 0 (19 %). The G+C content of the genome sequence of strain Marseille-P2666T is 64.6 mol%. On the basis of its phenotypic, phylogenetic and genomic features, strain Marseille-P2666T (=CSUR 2666T=DSM103342T) was classified as type strain of a novel species within the genus Collinsella for which the name Collinsella vaginalis sp. nov. is proposed.

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VAGINAL LACTOBACILLI INHIBITING GROWTH OF GARDNERELLA VAGINALIS, MOBILUNCUS AND OTHER BACTERIAL SPECIES CULTURED FROM VAGINAL CONTENT OF WOMEN WITH BACTERIAL VAGINOSIS

Acta Pathologica Microbiologica Scandinavica Series B Microbiology ◽

10.1111/j.1699-0463.1986.tb03074.x ◽

2009 ◽

Vol 94B (1-6) ◽

pp. 399-403 ◽

Cited By ~ 5

Author(s):

ANGELIKA Skarin ◽

JEANNE Sylwan

Keyword(s):

Bacterial Vaginosis ◽

Bacterial Species ◽

Gardnerella Vaginalis ◽

Vaginal Lactobacilli

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Quantitative PCR Assessments of Bacterial Species in Women with and without Bacterial Vaginosis

Journal of Clinical Microbiology ◽

10.1128/jcm.00851-09 ◽

2010 ◽

Vol 48 (5) ◽

pp. 1812-1819 ◽

Cited By ~ 136

Author(s):

M. Zozaya-Hinchliffe ◽

R. Lillis ◽

D. H. Martin ◽

M. J. Ferris

Keyword(s):

Bacterial Vaginosis ◽

Quantitative Pcr ◽

Bacterial Species

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Analysis of adherence, biofilm formation and cytotoxicity suggests a greater virulence potential of Gardnerella vaginalis relative to other bacterial-vaginosis-associated anaerobes

Microbiology ◽

10.1099/mic.0.034280-0 ◽

2010 ◽

Vol 156 (2) ◽

pp. 392-399 ◽

Cited By ~ 112

Author(s):

Jennifer L. Patterson ◽

Annica Stull-Lane ◽

Philippe H. Girerd ◽

Kimberly K. Jefferson

Keyword(s):

Bacterial Vaginosis ◽

Cytotoxic Activity ◽

Bacterial Species ◽

Opportunistic Pathogen ◽

Collective Effects ◽

Gardnerella Vaginalis ◽

Childbearing Age ◽

Virulence Potential ◽

Virulence Properties

Worldwide, bacterial vaginosis (BV) is the most common vaginal disorder in women of childbearing age. BV is characterized by a dramatic shift in the vaginal microflora, involving a relative decrease in lactobacilli, and a proliferation of anaerobes. In most cases of BV, the predominant bacterial species found is Gardnerella vaginalis. However, pure cultures of G. vaginalis do not always result in BV, and asymptomatic women are sometimes colonized with low numbers of G. vaginalis. Thus, there is controversy about whether G. vaginalis is an opportunistic pathogen and the causative agent of many cases of BV, or whether BV is a polymicrobial condition caused by the collective effects of an altered microbial flora. Recent studies of the biofilm-forming potential and cytotoxic activity of G. vaginalis have renewed interest in the virulence potential of this organism. In an effort to tease apart the aetiology of this disorder, we utilized in vitro assays to compare three virulence properties of G. vaginalis relative to other BV-associated anaerobes. We designed a viable assay to analyse bacterial adherence to vaginal epithelial cells, we compared biofilm-producing capacities, and we assessed cytotoxic activity. Of the BV-associated anaerobes tested, only G. vaginalis demonstrated all three virulence properties combined. This study suggests that G. vaginalis is more virulent than other BV-associated anaerobes, and that many of the bacterial species frequently isolated from BV may be relatively avirulent opportunists that colonize the vagina after G. vaginalis has initiated an infection.

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Prevalence of Bacterial Vaginosis among Married Women in Kalar District, Iraqi Kurdistan Region

passer ◽

10.24271/psr.32 ◽

2019 ◽

Vol 3 (2) ◽

pp. 194-199

Author(s):

Saman Mohammed Mohammed- Amin

Keyword(s):

Bacterial Vaginosis ◽

Pathogenic Bacteria ◽

Clinical Symptoms ◽

Micrococcus Luteus ◽

Bacterial Species ◽

Married Women ◽

P Value ◽

Klebsiella Pneumonia ◽

Microbiological Analysis ◽

Isolation And Identification

Abstract Bacterial vaginosis (BV) is an inflammatory disease, caused by polymicrobial infection, including pathogenic bacteria which replace the vaginal normal flora and finally this replacement causes manifestations of several physiological and clinical symptoms among women within different ages. BV has become one of the main problems that make woman patients visit gynecological and obstetric consultant hospitals in most country. The present study is designed to determine the causative pathogen and the prevalence of bacterial vaginosis among married women patients in Kalar district. This cross-sectional study was performed from the beginning of March to the mid April-2021 among women who attended Obstetrics and Gynecological governmental hospital and out-patient clinics in Kalar City. Intra vaginal swabs have been collected in sterile Amies transport medium sticks and processed for isolation and identification of bacterial species depending on colony morphology, Gram’s stain and microbiological analysis protocols. Then socio-demographic and gynaecologic data were collected by questionnaire. Out of the 108 participant women who suffered from Gynecological diseases, 67(62.03%) of them exhibited bacterial vaginosis. From the 73 different isolated colonies, 18 bacterial species were identified; coagulase-negative staphylococci (CoNS) were the predominant cause of BV (32.84%), followed by E. coli (14.93%), Staphylococcus aureus (13.43%), Klebsiella pneumonia (8.96) and Micrococcus luteus (7.46%), while Proteus spp. and some uncommon bacteria display (1.49%) for each of them. The socio-demographic analysis between positive and negative woman patients revealed that the association between all studied risk factors and BV were statistically significant (P value < 0.05) except the age factor which was statistically non-significant meaning that the age was not associated with BV. In addition, the clinical symptom analysis showed that abnormal vaginal discharge, lower back pain, dysmenorrhea and strawberry were significantly associated with BV (P value < 0.05), while the rest of other factor did not exhibit statistically significant association.

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Genital microbiota of sexual partners of female patients with bacterial vaginosis is responsible for its recurrences

Fundamental and Clinical Medicine ◽

10.23946/2500-0764-2020-5-3-66-76 ◽

2020 ◽

Vol 5 (3) ◽

pp. 66-76

Author(s):

S. V. Barinov ◽

V. A. Okhlopkov ◽

Yu. I. Tirskaya ◽

T. S.-K. Babaeva ◽

L. В. Sinel'nikova ◽

...

Keyword(s):

Treatment Group ◽

Bacterial Vaginosis ◽

Comparison Group ◽

Bacterial Species ◽

Local Delivery ◽

Sexual Partners ◽

Female Patients ◽

Female Partners ◽

Stage 1 ◽

Staphylococcus Spp

Aim. To evaluate the genital microbiota of sexual partners of women with bacterial vaginosis and its role in the development of its recurrences. Materials and Methods. We examined 59 consecutive patients with bacterial vaginosis. In the treatment group, sexual partners of the female patients were treated by local delivery of clindamycin; in the comparison group, sexual partners did not receive the drug. All women included in the study received the same therapy: metronidazole per os and local delivery of clindamycin at the stage 1 and local delivery of lactic acid at the stage 2. All patients were examined by microscopy, PCR test, and inoculation of the genital discharge. Monitoring of the female patients with bacterial vaginosis was continued for 6 months. Results. In male patients, genital microbiota was similar to their female partners. Gardnerella vaginalis was detected in 96% of females and 62% of males. Prevotella was identified in 93% of females and 41% of males while Atopobium vaginae was found in 79% of women and 48% of their sexual partners. The amount of bacterial species was similar in males and females of the treatment group. The most frequent microorganisms were Escherichia coli, Staphylococcus spp. and Enterococcus faecalis. Female patients of the treatment group had two-fold lower risk of recurrent bacterial vaginosis than those of the comparison group. Conclusion. Genital microbiota of sexual partners may be responsible for the recurrent bacterial vaginosis in females, and parallel treatment of sexual partners is required to minimize its risk.

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Draft Genome Sequence ofCriibacterium bergeroniigen. nov., sp. nov., Strain CCRI-22567T, Isolated from a Vaginal Sample from a Woman with Bacterial Vaginosis

Genome Announcements ◽

10.1128/genomea.00959-16 ◽

2016 ◽

Vol 4 (5) ◽

Author(s):

Andrée F. Maheux ◽

Ève Bérubé ◽

Dominique K. Boudreau ◽

Frédéric Raymond ◽

Jacques Corbeil ◽

...

Keyword(s):

Bacterial Vaginosis ◽

Genome Sequence ◽

Genome Assembly ◽

Type Strain ◽

New Genus ◽

Draft Genome ◽

Draft Genome Sequence ◽

Vaginal Sample

Criibacterium bergeroniigen. nov., sp. nov., CCRI-22567 is the type strain of the new genusCriibacterium. The strain was isolated from a woman with bacterial vaginosis. The genome assembly comprised 2,384,460 bp, with 34.4% G+C content. This is the first genome announcement of a strain belonging to the genusCriibacterium.

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