Hypothalamic nesfatin-1 mediates feeding behavior via MC3/4R-ERK signaling pathway after weight loss in obese Sprague-Dawley rats

Dehydroepiandrosterone (DHEA) is an endogenous steroid having a wide variety of biological and biochemical effects. In the present study, we have examined the role of DHEA on various rodent models of experimental hypertension. Sprague-Dawley rats were given subcutaneous injections of 1.5 mg dexamethasone every alternate day, resulting in an increase in systolic blood pressure within 1 wk. Interestingly, administration of a pharmacological dose of 1.5, 3, or 7.5 mg DHEA along with dexamethasone prevented dexamethasone-induced hypertension in a dose-dependent manner. DHEA had no effect on the hypertension induced by deoxycorticosterone acetate (DOCA)-salt administration using uninephrectomized rats or on the genetic model of spontaneously hypertensive rats. Dexamethasone administration resulted in a significant weight loss in rats, which was not prevented by simultaneous administration of DHEA. These results indicate that dexamethasone-mediated weight loss may involve mechanisms separate from its hypertensive action. Dexamethasone treatment resulted in a significant decrease in food consumption that was not reversed by DHEA. It is concluded that DHEA at doses above physiological levels when given subcutaneously has no effect on DOCA-salt or a genetic model of hypertension but has a beneficial effect on dexamethasone-induced hypertension.

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Prevention of stress-induced weight loss by third ventricle CRF receptor antagonist

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1999.276.5.r1461 ◽

1999 ◽

Vol 276 (5) ◽

pp. R1461-R1468 ◽

Cited By ~ 15

Author(s):

Gennady N. Smagin ◽

Leigh Anne Howell ◽

Stephen Redmann ◽

Donna H. Ryan ◽

Ruth B. S. Harris

Keyword(s):

Weight Loss ◽

Body Weight ◽

Receptor Antagonist ◽

Acute Stress ◽

Third Ventricle ◽

Corticotropin Releasing Factor ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Sustained Reduction ◽

Acute Responses

We previously reported that rats exposed to repeated restraint (3 h/day for 3 days) experience temporary hypophagia and a sustained reduction in body weight compared with nonrestrained controls. Studies described here determined the involvement of central corticotropin-releasing factor (CRF) receptors in the initiation of this chronic response to acute stress. In experiment 1, Sprague-Dawley rats were fitted with cannulas in the lateral ventricle and infused with 50 μg of αhCRF-(9—41) or saline immediately before restraint on each of the 3 days of restraint. The receptor antagonist inhibited hypophagia and weight loss on day 1 of restraint but not on days 2 and 3. In experiment 2, 10 μg of αhCRF-(9—41) or saline were infused into the third ventricle immediately before each restraint. The receptor antagonist totally blocked stress-induced hypophagia and weight loss. These results demonstrate that CRF receptors located in or near the hypothalamus mediate the acute responses to stress that lead to a permanent change in the hormonal or metabolic processes that determine body weight and body composition.

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Larrad Biliopancreatic Diversion in Sprague-Dawley Rats. Analysis of Weight Loss Related to Food Intake

Obesity Surgery ◽

10.1007/s11695-008-9710-9 ◽

2008 ◽

Vol 19 (4) ◽

pp. 484-489 ◽

Cited By ~ 6

Author(s):

Hugo Mendieta-Zerón ◽

Álvaro Larrad-Jiménez ◽

Gema Frühbeck ◽

Katia Da Boit ◽

C. Diéguez

Keyword(s):

Weight Loss ◽

Food Intake ◽

Biliopancreatic Diversion ◽

Sprague Dawley ◽

Sprague Dawley Rats

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eNAMPT neutralization reduces preclinical ARDS severity via rectified NFkB and Akt/mTORC2 signaling

Scientific Reports ◽

10.1038/s41598-021-04444-9 ◽

2022 ◽

Vol 12 (1) ◽

Author(s):

Tadeo Bermudez ◽

Saad Sammani ◽

Jin H. Song ◽

Vivian Reyes Hernon ◽

Carrie L. Kempf ◽

...

Keyword(s):

Lung Injury ◽

Signaling Pathway ◽

Unmet Need ◽

Serum Lactate ◽

Sprague Dawley ◽

Nicotinamide Phosphoribosyltransferase ◽

Molecular Pattern ◽

Sprague Dawley Rats ◽

Pathway Activation ◽

Nfkb Pathway

AbstractDespite encouraging preclinical data, therapies to reduce ARDS mortality remains a globally unmet need, including during the COVID-19 pandemic. We previously identified extracellular nicotinamide phosphoribosyltransferase (eNAMPT) as a novel damage-associated molecular pattern protein (DAMP) via TLR4 ligation which regulates inflammatory cascade activation. eNAMPT is tightly linked to human ARDS by biomarker and genotyping studies in ARDS subjects. We now hypothesize that an eNAMPT-neutralizing mAb will significantly reduce the severity of ARDS lung inflammatory lung injury in diverse preclinical rat and porcine models. Sprague Dawley rats received eNAMPT mAb intravenously following exposure to intratracheal lipopolysaccharide (LPS) or to a traumatic blast (125 kPa) but prior to initiation of ventilator-induced lung injury (VILI) (4 h). Yucatan minipigs received intravenous eNAMPT mAb 2 h after initiation of septic shock and VILI (12 h). Each rat/porcine ARDS/VILI model was strongly associated with evidence of severe inflammatory lung injury with NFkB pathway activation and marked dysregulation of the Akt/mTORC2 signaling pathway. eNAMPT neutralization dramatically reduced inflammatory indices and the severity of lung injury in each rat/porcine ARDS/VILI model (~ 50% reduction) including reduction in serum lactate, and plasma levels of eNAMPT, IL-6, TNFα and Ang-2. The eNAMPT mAb further rectified NFkB pathway activation and preserved the Akt/mTORC2 signaling pathway. These results strongly support targeting the eNAMPT/TLR4 inflammatory pathway as a potential ARDS strategy to reduce inflammatory lung injury and ARDS mortality.

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Intraperitoneal injection of PDTC on the NF-kB signaling pathway and osteogenesis indexes of young adult rats with anterior palatal suture expansion model

PLoS ONE ◽

10.1371/journal.pone.0243108 ◽

2021 ◽

Vol 16 (7) ◽

pp. e0243108

Author(s):

Yafang Li ◽

Yiqiang Qiao ◽

Huanhuan Wang ◽

Zao Wang

Keyword(s):

Young Adult ◽

Signaling Pathway ◽

Intraperitoneal Injection ◽

Control Group ◽

Sprague Dawley ◽

Adult Rats ◽

Morphogenetic Protein ◽

Sprague Dawley Rats ◽

The Difference ◽

Expansion Model

In recent years, many studies have found that mechanical tension can activiate NF-kB signal pathway and NF-kB plays an important role in the process of osteogenesis. However, it is still unclear whether this process exists in the anterior palatal suture expansion. In this paper, we mainly studied the effect of intraperitoneal injection of PDTC on the NF-kB signaling pathway and osteogenesis index of the anterior palatal suture expansion model in young adult rats. The expansion model is grouped and established: 45 male 8-week-old Sprague-Dawley rats were randomly divided into three groups, an expansion only (EO) group, an expansion plus PDTC (PE) group, and a control group. The results revealed that PDTC inhibited the activity of NF-kB signaling pathway and promote one morphogenetic protein 2 (BMP-2), steocalatin (OCN) expression. Compared with the control group, the optical density (OD) value of BMP in the EO group and PE group rats increased significantly from the first day to the seventh day, and the difference was statistically significant (P<0.05). After 6.0Gy irradiation, PDTC administration group could slightly increase the total SOD level in the liver and serum of rats, and reduce the MDA level in the liver and serum, especially the effect of 60mg/kg and 90mg/kg was the most obvious.

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Developmental exposure to paraquat and maneb can impair cognition, learning and memory in Sprague-Dawley rats

Molecular BioSystems ◽

10.1039/c6mb00284f ◽

2016 ◽

Vol 12 (10) ◽

pp. 3088-3097 ◽

Cited By ~ 15

Author(s):

Bai Li ◽

Xi He ◽

Yan Sun ◽

Baixiang Li

Keyword(s):

Cognitive Function ◽

Signaling Pathway ◽

Learning And Memory ◽

Sprague Dawley ◽

Developmental Exposure ◽

Rat Offspring ◽

Sprague Dawley Rats

PQ–MB may affect the behavior and cognitive function of rat offspring through the cAMP–PKA–CREB signaling pathway.

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Dietary Exposure to Oxidized Frying Oil from Fetus to Adulthood Suppresses Male Reproductive Development by Altering Testicular Cholesterol and Testosterone Homeostasis in Sprague Dawley Rats

Journal of Nutrition ◽

10.1093/jn/nxaa091 ◽

2020 ◽

Vol 150 (7) ◽

pp. 1713-1721

Author(s):

Hai-Ping Wu ◽

Yu-Shun Lin ◽

Chi-Fen Chang ◽

Shui-Yuan Lu ◽

Pei-Min Chao

Keyword(s):

Weight Loss ◽

Body Weight ◽

Soybean Oil ◽

Body Weight Loss ◽

Reproductive Development ◽

Frying Oil ◽

Female Rats ◽

Male Rats ◽

Sprague Dawley ◽

Sprague Dawley Rats

ABSTRACT Background Dietary frying oil may have endocrine-disrupting effects, as a feminization effect was observed in cohorts of C57BL/6J male mice fetuses from dams consuming oxidized frying oil (OFO) during pregnancy. Objective The aim of present study was to test the hypothesis that OFO is an anti-androgen. Methods In experiment 1, male progeny of Sprague Dawley female rats fed fresh oil or an OFO diet (10 g fat/100 g, from fresh or 24-h–fried soybean oil; [control diet (C) and OFO groups, respectively] from midgestation through lactation were studied. Pups were weaned at 3 wk of age and then consumed their mothers’ diet until 9 wk of age. In addition, a group of dams and pups that consumed a high-fat diet (HF; 10 g fried and 20 g fresh soybean oil/100 g) was included to counteract body-weight loss associated with OFO ingestion. Indices of male reproductive development and testosterone homeostasis were measured. In experiment 2, male rats were allocated to C and OFO groups (treated as above) and indices of male fertility compared at 9–10 wk of age. Results In experiment 1, final body weights of the HF group were lower (17%) than the C group but higher (14%) than the OFO group (P < 0.0001 for each). In addition to abnormalities in seminiferous tubules, HF and OFO groups did not differ from one another, but, compared with the C group, had delayed preputial separation (4.9 d) and reductions in serum testosterone concentrations (17–74%), anogenital distance (8–20%), weights of androgen-dependent tissues (8–30%), testicular testosterone and cholesterol concentrations (30–40%), and mRNA levels of genes involved in steroidogenesis and cholesterol homeostasis (30–70%). In experiment 2, OFO-exposed males had 20% lower sperm motility (P < 0.05); however, when mated to normal females, pregnancy rates and litter sizes did not differ between OFO and C groups. Conclusions The anti-androgenic effect of OFO in Sprague Dawley rats was attributed to decreased testicular concentrations of cholesterol (testosterone precursor) and not body-weight loss.

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Effect of dietary restriction and/or exercise on 23-h metabolic rate and body composition in female rats

Journal of Applied Physiology ◽

10.1152/jappl.1991.71.3.801 ◽

1991 ◽

Vol 71 (3) ◽

pp. 801-806 ◽

Cited By ~ 19

Author(s):

D. L. Ballor

Keyword(s):

Weight Loss ◽

Body Composition ◽

Dietary Intake ◽

Metabolic Rate ◽

Resting Metabolic Rate ◽

Normal Weight ◽

Female Rats ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Heart Mass

This study examined the effects of three levels of dietary intake [ad libitum fed (AL), moderately severe (MSR), and severe restriction (SR)] and two levels of exercise [cage confinement (CC) and exercise training (E)] on 23-h resting metabolic rate (RMR) and body composition in 47 female Sprague-Dawley rats. At the end of the 9-wk study, the MSR and SR groups weighed approximately 81 and 61%, respectively, of the AL-CC group. RMR was depressed for the MSR and SR groups compared with the AL-CC group. This was true whether expressed on an absolute (ml/min) or relative (ml.min-1.kg-0.75) basis. On a relative basis, which accounts for changes caused by weight loss alone, the RMR decreased by approximately 12 and 19%, respectively, for the MSR and SR groups compared with the AL-CC group. Although E resulted in significant differences in fat mass, percent fat, percent water, and heart mass between the AL groups, there were no significant differences between E and CC groups at either the MSR or SR level of dietary intake for any of the variables measured (i.e., body composition, muscle mass, RMR). Thus E does not appear to affect the composition of lost weight or RMR during diet-induced weight loss for female rats of normal weight.

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