Inhibition of the catalase activity from Phaseolus vulgaris and Medicago sativa by sodium chloride

2007 ◽  
Vol 45 (8) ◽  
pp. 535-541 ◽  
Author(s):  
Noel A. Tejera García ◽  
Carmen Iribarne ◽  
Francisco Palma ◽  
Carmen Lluch
2015 ◽  
Vol 1130 ◽  
pp. 598-601
Author(s):  
Sara Liz Pacheco ◽  
Patricia Sheen ◽  
Jasminc Hurtado

Three isolates ofStreptomyces variabilis(AB5, F and K1A) and one isolate ofStreptomyces sp.(C2) were isolated of acidic cultures from mineral ores and concentrates from mines of the Peruvian highlands. The isolates were identified molecular and phenotypically and characterized by growth at different conditions as pH tolerance, temperature, and sodium chloride and heavy metals resistance.Streptomyces sp. (C2) was able to grow until 200 ppm of mercury andS. variabilis(AB5) grows in the presence of 100 ppm of mercury. The isolates AB5 and K1A ofS. variabiliswere able to grow only until 50 ppm of mercury.All strains were tested by their ability to induce germination ofLactuca sativa,Medicago sativaandPhaseolus vulgarisseeds exposed to different mercury concentrations. It was shown a significative improvement inL.sativaandP. vulgaris. InL. sativaexposed to 50 ppm of mercury, all strains were able to improve germination, root length and number of leaves. Without Streptomyces addition, they only growth until 10 ppm of mercury.Streptomyces sp.isolatehad better results in germination, root length and number of leaves ofL. sativa.The results found in this work indicate the potential of these isolates for restoration of soils polluted with mercury.


2016 ◽  
Vol 4 (6) ◽  
pp. 222-228
Author(s):  
Roba Tufa ◽  
Jima Nego

This study was conducted at Jimma University College of Agriculture and Veterinary Medicine (JUCAVM) under controlled environmental conditions of greenhouse to determine the effects of different concentration of sodium chloride (NaCl) on germination and seedling growth of Phaseolus vulgaris L. Five concentrations of sodium chloride (o mol(distilled water), 0.1M, 0.2M,0.3M and 0.4M) with one control treatment of un-primed seeds were laid out  in completely randomized design (CRD) with three replication. Data were collected on standard germination, Rate of germination, Seedling height, Shoot length, Root length and Vigor index. The result revealed that different NaCl concentrations statistically (p≤0.05) affected standard germination, Rate of germination, Seedling height, Shoot length and Vigor index I of common bean crop. The highest value of all these parameters were obtained from seed primed with 0.1M NaCl as well as when the seed primed with distilled water. However, the lowest values of these parameters were recorded on the seed primed with the highest concentration (0.4M) of NaCl and un-primed seed. More of the examined parameters were decreased with increasing of NaCl concentration. In conclusion, priming seed with 0.1M of NaCl concentration enhanced germination and seedling performance of common bean.


HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 427F-428 ◽  
Author(s):  
Kwang-Hyun Baek ◽  
C.B. Rajashekar

Effects of hypoxia on germinating bean seeds (Phaseolus vulgaris cv. Tendergreen) were examined by imbibing them in water for various lengths of time. Hypocotyl elongation under hypoxic conditions and recovery from hypoxia in bean seeds were determined. Oxygen concentration in the water began to decrease sharply after 12 h of seed imbibition and had declined by more than 63% after 3 days of seed imbibition. When seeds were germinated on 0.8% agar after 24 h of imbibition, the hypocotyl elongation was reduced by about 70% compared to the seeds with no hypoxia, and longer imbibition resulted in poor or no germination. Exogenous hydrogen peroxide (20 mm) in water increased the oxygen concentration from 250 to 350 mm in the presence of seeds and was considerably higher after 3 days of seed imbibition than that in the control. Hypocotyl elongation occurred in seeds submerged in water containing hydrogen peroxide up to 72 h while none was observed in water. This was comparable to hypocotyl elongation under non-hypoxic conditions. Hypoxia in imbibing seeds was overcome by the high oxygen levels in the medium resulting from reaction of hydrogen peroxide with seed catalase and catalytic metal ions. Considerable catalase activity was detected in germinating seeds and the use of a catalase inhibitor, aminotriazole, suggests that the enzyme plays an important role in the release of oxygen into the medium. Of the catalytic metals, the seed content of iron was dominant and was about 6 folds higher than that of either copper or manganese.


Plant Science ◽  
1993 ◽  
Vol 89 (1) ◽  
pp. 81-84 ◽  
Author(s):  
S.H. Shah ◽  
S.J. Wainwright ◽  
M.J. Merrett

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Mamane Sani ◽  
Hichem Sebai ◽  
Roberto Refinetti ◽  
Mohan Mondal ◽  
Néziha Ghanem-Boughanmi ◽  
...  

To investigate the time dependence of sodium nitroprusside- (NPS-) induced oxidative effects, the authors study the variation of the antioxidant enzyme CAT activity in various tissues after the administration of a single 2.5 mg/kg dose of SNP or sodium chloride (NaCl 0.9%). For each of the two dosing times (1 and 13 hours after light onset, HALO, which correspond to the beginning of diurnal rest span and of nocturnal activity span of mice, resp.), brain, kidney, and liver tissues were excised from animals at 0, 1, 3, 6, 9, 12, 24, and 36 h following the drug administration and CAT activity was assayed. The results suggest that SNP-induced stimulation of CAT activity is greater in all three tissues when the drug is administered at 1 HALO than at 13 HALO. Two-way ANOVA revealed that CAT activity significantly (P<0.004) varied as a function of the sampling time but not of the treatment in all three tissues. Moreover, a statistically significant (P<0.004) interaction between the organ sampling-time and the SNP treatment was revealed in kidney regardless of the dosing time, whereas a highly significant (P<0.0002) interaction was validated in liver only in animals injected at 13 HALO.


Weed Science ◽  
1974 ◽  
Vol 22 (3) ◽  
pp. 197-200
Author(s):  
E. Basler ◽  
F. W. Slife

The effects of chloride salts of potassium, sodium, ammonium, and lithium and abscisic acid (ABA) on the translocation of 2,4,5-T [(2,4,5-trichlorophenoxy)acetic acid] in bean (Phaseolus vulgarisL. Var. Stringless Green-pod) seedlings were determined.14C-labeled 2,4,5-T was injected in the stem tissue at the cotyledonary node in a 1-μl volume along with ABA. The salts were added to the nutrient solution at 20 mM concentrations at the time of 2,4,5-T treatment. Sodium chloride inhibited 2,4,5-T accumulation in the upper portion of the plant; and except for LiCl, the salts tended to increase accumulation in the nutrient solution. LiCl increased 2,4,5-T accumulation in the young shoots and primary leaves and decreased accumulation in the roots and nutrient solution. The effects of ABA on 2,4,5-T translocation were similar to the effects of NaCl but were more pronounced. ABA caused large decreases in 2,4,5-T translocation to the young shoots and primary leaves and increased accumulation in the nutrient solution.


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