Ectopic expression of dehydration responsive element binding proteins (StDREB2) confers higher tolerance to salt stress in potato

Background/Aims: The conformation, folding and lipid binding properties of the intestinal fatty acid binding proteins (IFABP) have been extensively investigated. In contrast, the functional aspects of these proteins are not understood and matter of debates. In this study, we aim to address the deleterious effects of FA overload on cellular components, particularly mitochondria; and how IFABP helps in combating this stress by restoring the mitochondrial dynamics. Methods: In the present study the functional aspect of IFABP under conditions of lipid stress was studied by a string of extensive in-cell studies; flow cytometry by fluorescence-activated cell sorting (FACS), confocal imaging, western blotting and quantitative real time PCR. We deployed ectopic expression of IFABP in rescuing cells under the condition of lipid stress. Again in order to unveil the mechanistic insights of functional traits, we arrayed extensive computational approaches by means of studying centrality calculations along with protein-protein association and ligand induced cluster dissociation. While addressing its functional importance, we used FCS and in-silico computational analyses, to show the structural distribution and the underlying mechanism of IFABP’s action. Results: Ectopic expression of IFABP in HeLa cells has been found to rescue mitochondrial morphological dynamics and restore membrane potential, partially preventing apoptotic damage induced by the increased FAs. These findings have been further validated in the functionally relevant intestinal Caco-2 cells, where the native expression of IFABP protects mitochondrial morphology from abrogation induced by FA overload. However, this native level expression is insufficient to protect against apoptotic cell death, which is rescued, at least partially in cells overexpressing IFABP. In addition, shRNA mediated IFABP knockdown in Caco-2 cells compromises mitochondrial dynamics and switches on intrinsic apoptotic pathways under FA-induced metabolic stress. Conclusion: To summarize, the present study implicates functional significance of IFABP in controlling ligand-induced damage in mitochondrial dynamics and apoptosis.

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High accumulation of anthocyanins via the ectopic expression of AtDFR confers significant salt stress tolerance in Brassica napus L.

Plant Cell Reports ◽

10.1007/s00299-017-2147-7 ◽

2017 ◽

Vol 36 (8) ◽

pp. 1215-1224 ◽

Cited By ~ 16

Author(s):

Jihye Kim ◽

Won Je Lee ◽

Tien Thanh Vu ◽

Chan Young Jeong ◽

Suk-Whan Hong ◽

...

Keyword(s):

Salt Stress ◽

Brassica Napus ◽

Stress Tolerance ◽

Ectopic Expression ◽

Salt Stress Tolerance ◽

Brassica Napus L ◽

High Accumulation

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Isolation and characterization of two DREB1 genes encoding dehydration-responsive element binding proteins in chicory (Cichorium intybus)

Plant Growth Regulation ◽

10.1007/s10725-013-9866-8 ◽

2013 ◽

Vol 73 (1) ◽

pp. 45-55 ◽

Cited By ~ 9

Author(s):

Mingxiang Liang ◽

Dandan Chen ◽

Manman Lin ◽

Qingsong Zheng ◽

Zengrong Huang ◽

...

Keyword(s):

Binding Proteins ◽

Cichorium Intybus ◽

Isolation And Characterization ◽

Genes Encoding ◽

Responsive Element

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Ectopic expression of SbNHX1 gene in transgenic castor (Ricinus communis L.) enhances salt stress by modulating physiological process

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-015-0785-4 ◽

2015 ◽

Vol 122 (2) ◽

pp. 477-490 ◽

Cited By ~ 41

Author(s):

Manish Kumar Patel ◽

Mukul Joshi ◽

Avinash Mishra ◽

Bhavanath Jha

Keyword(s):

Salt Stress ◽

Ricinus Communis ◽

Ectopic Expression ◽

Physiological Process ◽

Ricinus Communis L ◽

Transgenic Castor

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Expression of cAMP-responsive element binding proteins (CREBs) in fast- and slow-twitch muscles: A signaling pathway to account for the synaptic expression of collagen-tailed subunit (ColQ) of acetylcholinesterase at the rat neuromuscular junction

Chemico-Biological Interactions ◽

10.1016/j.cbi.2012.10.025 ◽

2013 ◽

Vol 203 (1) ◽

pp. 282-286 ◽

Cited By ~ 4

Author(s):

Roy C.Y. Choi ◽

Vicky P. Chen ◽

Wilson K.W. Luk ◽

Amanda W.Y. Yung ◽

Alice H.M. Ng ◽

...

Keyword(s):

Neuromuscular Junction ◽

Signaling Pathway ◽

Binding Proteins ◽

Slow Twitch ◽

Responsive Element ◽

Camp Responsive Element Binding

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delilah, prospero and D-Pax2 constitute a gene regulatory network essential for the development of functional proprioceptors

10.1101/2021.04.07.438778 ◽

2021 ◽

Author(s):

Adel Avetisyan ◽

Yael Glatt ◽

Maya Cohen ◽

Yael Timerman ◽

Nitay Aspis ◽

...

Keyword(s):

Gene Regulatory Network ◽

Regulatory Network ◽

Regulatory Element ◽

Ectopic Expression ◽

Chordotonal Organ ◽

Specific Expression ◽

Regulatory Module ◽

Gene Regulatory ◽

Responsive Element ◽

Do So

In this work we describe a gene regulatory network consisting of three transcription factors- Prospero (Pros), D-Pax2/Shaven (Sv) and Delilah/Taxi (Dei/Tx)- that dictates two alternative differentiation programs within the proprioceptive lineage in Drosophila. D-Pax2 and Pros control the differentiation of cap versus scolopale cells in the chordotonal organ lineage by, respectively, activating and repressing the transcription of dei. Normally, D-Pax2 activates the expression of dei in the cap cell but is unable to do so in the scolopale cell where Pros is co-expressed. If D-Pax2 activity is lost, the cap cell fails to express dei as well as additional proteins, such as αTub85E, that characterize a fully differentiated cap cell. In contrast, if Pros activity is lost, dei is ectopically expressed in the scolopale cell that, as a consequence, adopts some cap cell features, including the expression of αTub85E. We further show that D-Pax2 and Pros exert their effects on dei transcription via a 262 bp chordotonal-specific regulatory module (deiChO-262) in which two D-Pax2- and three Pros-binding sites were identified experimentally. When this regulatory element was removed from the fly genome, the cap- and ligament-specific expression of dei was lost. Ectopic expression of D-Pax2, or the elimination of pros activity, did not lead to upregulation of dei in the scolopale cell in the absence of the D-Pax2-responsive deiChO-262 enhancer. Finally, we show that the regulation of dei expression via the D-Pax2/Pros-responsive element is critical for chordotonal organ functionality and coordinated larval locomotion.

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Activation of Sterol-Responsive Element Binding Proteins in a Sucrose- Induced Model of Lysosomal Storage

The Open Pathology Journal ◽

10.2174/1874375720130905003 ◽

2013 ◽

Vol 7 (1) ◽

pp. 16-27

Author(s):

Guillaume Van Beersel

Keyword(s):

Binding Proteins ◽

Lysosomal Storage ◽

Responsive Element

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RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops

Agronomy ◽

10.3390/agronomy10020250 ◽

2020 ◽

Vol 10 (2) ◽

pp. 250 ◽

Cited By ~ 2

Author(s):

Sara Rosa Téllez ◽

Rodoldphe Kanhonou ◽

Carlos Castellote Bellés ◽

Ramón Serrano ◽

Paula Alepuz ◽

...

Keyword(s):

Salt Stress ◽

Salt Tolerance ◽

Sugar Beet ◽

Rna Splicing ◽

Yeast Strain ◽

Binding Proteins ◽

Rna Binding ◽

Rna Binding Proteins ◽

Crop Improvement ◽

Salt Toxicity

Salt stress drastically reduce crop productivity. In order to identify genes that could improve crop salt tolerance, we randomly expressed a cDNA library of the halotolerant sugar beet in a sodium-sensitive yeast strain. We identified six sugar beet genes coding for RNA binding proteins (RBP) able to increase the yeast Na+-tolerance. Two of these genes, named Beta vulgaris Salt Tolerant 3 (BvSATO3) and BvU2AF35b, participate in RNA splicing. The other four BvSATO genes (BvSATO1, BvSATO2, BvSATO4 and BvSATO6) are putatively involved in other processes of RNA metabolism. BvU2AF35b improved the growth of a wild type yeast strain under salt stress, and also in mutant backgrounds with impaired splicing, thus confirming that splicing is a target of salt toxicity. To validate the yeast approach, we characterized BvSATO1 in sugar beet and Arabidopsis. BvSATO1 expression was repressed by salt treatment in sugar beet, suggesting that this gene could be a target of salt toxicity. Expression of BvSATO1 in Arabidopsis increased the plant salt tolerance. Our results suggest that not only RNA splicing, but RNA metabolic processes such as such as RNA stability or nonsense-mediated mRNA decay may also be affected by salt stress and could be biotechnological targets for crop improvement.

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