Identification of the wheat C3H gene family and expression analysis of candidates associated with seed dormancy and germination

2020 ◽  
Vol 156 ◽  
pp. 524-537
Author(s):  
Xinran Cheng ◽  
Jiajia Cao ◽  
Chang Gao ◽  
Wei Gao ◽  
Shengnan Yan ◽  
...  
Keyword(s):  
Gene Family ◽  
Seed Dormancy ◽  
Expression Analysis ◽  
Seed Dormancy And Germination

scholarly journals Identification of the Wheat VQ Protein Family and Expression Analysis of Candidate Genes Associated with Seed Dormancy and Germination

2020 ◽  
Author(s):  
Xinran Cheng ◽  
Chang Gao ◽  
Xue Liu ◽  
Dongmei Xu ◽  
Xu Pan ◽  
...  
Keyword(s):  
Seed Germination ◽  
Candidate Genes ◽  
Common Wheat ◽  
Seed Dormancy ◽  
Expression Analysis ◽  
Evolutionary Divergence ◽  
Wheat Varieties ◽  
Grain Yield And Quality ◽  
Sprouting Resistance ◽  
Seed Dormancy And Germination

Abstract Background: Seed dormancy and germination determine wheat pre-harvest sprouting resistance and thereby affect grain yield and quality. Arabidopsis VQ genes have been shown to influence seed germination; however, the functions of wheat VQ genes have not been characterized. Results: In this study, we identified 65 TaVQ genes in common wheat and named them TaVQ1–65. We identified 48 paralogous pairs, 37 of which had Ka/Ks values lager than 1, suggesting that most TaVQ genes have suffer positive selection. Chromosome location, gene structure, promoter element and gene ontology annotation showed that the structure of the genes determined their function and that structural change reflected functional diversity. The transcriptome expression analysis of 62 TaVQ genes and microarray analysis of 11 TaVQ genes indicated that they played important roles in diverse biological processes. We compared TaVQ gene expression and corresponding seed germination index values among wheat varieties with contrasting seed dormancy and germination phenotypes and found that 21 TaVQ genes may be related to seed dormancy and germination. Conclusions: Sixty-five TaVQ proteins were identified for the first time in common wheat, and bioinformatics analysis was performed to investigate their phylogenetic relationships and evolutionary divergence. The qRT-PCR data showed that 21 TaVQ candidate genes were potentially involved in seed dormancy and germination. These findings provide effective information for further cloning and functional analysis of TaVQ genes, as well as useful candidate genes for improvement of PHS resistance in wheat.

Expression Analysis of Genes Involved in Peanut Seed Dormancy Release (Arachis hypogaeaL.)

2015 ◽  
Vol 41 (6) ◽  
pp. 845 ◽  
Author(s):  
Jing CHEN ◽  
Ling JIANG ◽  
Chun-Ming WANG ◽  
Xiao-Hui HU ◽  
Hu-Qu ZHAI ◽  
...  
Keyword(s):  
Seed Dormancy ◽  
Expression Analysis ◽  
Dormancy Release ◽  
Peanut Seed

scholarly journals Genome-wide identification of cyclin-dependent kinase (CDK) genes affecting adipocyte differentiation in cattle

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Cuili Pan ◽  
Zhaoxiong Lei ◽  
Shuzhe Wang ◽  
Xingping Wang ◽  
Dawei Wei ◽  
...  
Keyword(s):  
Gene Family ◽  
Expression Analysis ◽  
Adipocyte Differentiation ◽  
Bos Taurus ◽  
Adipogenic Differentiation ◽  
Critical Role ◽  
Bos Indicus ◽  
Specific Expression ◽  
Genome Wide ◽  
A Genome

Abstract Background Cyclin-dependent kinases (CDKs) are protein kinases regulating important cellular processes such as cell cycle and transcription. Many CDK genes also play a critical role during adipogenic differentiation, but the role of CDK gene family in regulating bovine adipocyte differentiation has not been studied. Therefore, the present study aims to characterize the CDK gene family in bovine and study their expression pattern during adipocyte differentiation. Results We performed a genome-wide analysis and identified a number of CDK genes in several bovine species. The CDK genes were classified into 8 subfamilies through phylogenetic analysis. We found that 25 bovine CDK genes were distributed in 16 different chromosomes. Collinearity analysis revealed that the CDK gene family in Bos taurus is homologous with Bos indicus, Hybrid-Bos taurus, Hybrid Bos indicus, Bos grunniens and Bubalus bubalis. Several CDK genes had higher expression levels in preadipocytes than in differentiated adipocytes, as shown by RNA-seq analysis and qPCR, suggesting a role in the growth of emerging lipid droplets. Conclusion In this research, 185 CDK genes were identified and grouped into eight distinct clades in Bovidae, showing extensively homology. Global expression analysis of different bovine tissues and specific expression analysis during adipocytes differentiation revealed CDK4, CDK7, CDK8, CDK9 and CDK14 may be involved in bovine adipocyte differentiation. The results provide a basis for further study to determine the roles of CDK gene family in regulating adipocyte differentiation, which is beneficial for beef quality improvement.

scholarly journals Regulation of Seed Dormancy and Germination Mechanisms in a Changing Environment

2021 ◽  
Vol 22 (3) ◽  
pp. 1357
Author(s):  
Ewelina A. Klupczyńska ◽  
Tomasz A. Pawłowski
Keyword(s):  
Climate Change ◽  
Seed Germination ◽  
Seed Dormancy ◽  
Environmental Conditions ◽  
Global Climate ◽  
Plant Evolution ◽  
Suitable Habitat ◽  
Climate Change Scenarios ◽  
Adaptation Mechanism ◽  
Seed Dormancy And Germination

Environmental conditions are the basis of plant reproduction and are the critical factors controlling seed dormancy and germination. Global climate change is currently affecting environmental conditions and changing the reproduction of plants from seeds. Disturbances in germination will cause disturbances in the diversity of plant communities. Models developed for climate change scenarios show that some species will face a significant decrease in suitable habitat area. Dormancy is an adaptive mechanism that affects the probability of survival of a species. The ability of seeds of many plant species to survive until dormancy recedes and meet the requirements for germination is an adaptive strategy that can act as a buffer against the negative effects of environmental heterogeneity. The influence of temperature and humidity on seed dormancy status underlines the need to understand how changing environmental conditions will affect seed germination patterns. Knowledge of these processes is important for understanding plant evolution and adaptation to changes in the habitat. The network of genes controlling seed dormancy under the influence of environmental conditions is not fully characterized. Integrating research techniques from different disciplines of biology could aid understanding of the mechanisms of the processes controlling seed germination. Transcriptomics, proteomics, epigenetics, and other fields provide researchers with new opportunities to understand the many processes of plant life. This paper focuses on presenting the adaptation mechanism of seed dormancy and germination to the various environments, with emphasis on their prospective roles in adaptation to the changing climate.

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