Integrated transcriptomics and metabolomics provide novel insight into changes in specialized metabolites in an albino tea cultivar (Camellia sinensis (L.) O. Kuntz)

2021 ◽  
Author(s):  
Yucheng Zheng ◽  
Pengjie Wang ◽  
Xuejin Chen ◽  
Chuan Yue ◽  
Yongchun Guo ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Specialized Metabolites ◽  
Tea Cultivar ◽  
Insight Into

Insight into Catechins Metabolic Pathways of Camellia sinensis Based on Genome and Transcriptome Analysis

2018 ◽  
Vol 66 (16) ◽  
pp. 4281-4293 ◽  
Author(s):  
Wenzhao Wang ◽  
Yihui Zhou ◽  
Yingling Wu ◽  
Xinlong Dai ◽  
Yajun Liu ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Transcriptome Analysis ◽  
Metabolic Pathways ◽  
Insight Into

scholarly journals Metabolism of Gallic Acid and Its Distributions in Tea (Camellia sinensis) Plants at the Tissue and Subcellular Levels

2020 ◽  
Vol 21 (16) ◽  
pp. 5684 ◽  
Author(s):  
Xiaochen Zhou ◽  
Lanting Zeng ◽  
Yingjuan Chen ◽  
Xuewen Wang ◽  
Yinyin Liao ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Camellia Sinensis ◽  
Tea Plant ◽  
Plant Diseases ◽  
Tea Leaves ◽  
Specialized Metabolites ◽  
Tea Plants ◽  
Mg Content ◽  
Ga Content

In tea (Camellia sinensis) plants, polyphenols are the representative metabolites and play important roles during their growth. Among tea polyphenols, catechins are extensively studied, while very little attention has been paid to other polyphenols such as gallic acid (GA) that occur in tea leaves with relatively high content. In this study, GA was able to be transformed into methyl gallate (MG), suggesting that GA is not only a precursor of catechins, but also can be transformed into other metabolites in tea plants. GA content in tea leaves was higher than MG content—regardless of the cultivar, plucking month or leaf position. These two metabolites occurred with higher amounts in tender leaves. Using nonaqueous fractionation techniques, it was found that GA and MG were abundantly accumulated in peroxisome. In addition, GA and MG were found to have strong antifungal activity against two main tea plant diseases, Colletotrichum camelliae and Pseudopestalotiopsis camelliae-sinensis. The information will advance our understanding on formation and biologic functions of polyphenols in tea plants and also provide a good reference for studying in vivo occurrence of specialized metabolites in economic plants.

Effect of Major Tea Insect Attack on Formation of Quality-Related Nonvolatile Specialized Metabolites in Tea (Camellia sinensis) Leaves

2019 ◽  
Vol 67 (24) ◽  
pp. 6716-6724 ◽  
Author(s):  
Yinyin Liao ◽  
Zhenming Yu ◽  
Xiaoyu Liu ◽  
Lanting Zeng ◽  
Sihua Cheng ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Specialized Metabolites ◽  
Insect Attack

scholarly journals Trichlorination of a Teicoplanin-Type Glycopeptide Antibiotic by the Halogenase StaI Evades Resistance

2018 ◽  
Vol 62 (12) ◽  
Author(s):  
Grace Yim ◽  
Wenliang Wang ◽  
Andrew C. Pawlowski ◽  
Gerard D. Wright
Keyword(s):  
Biological Function ◽  
Antibiotic Activity ◽  
Important Class ◽  
Glycopeptide Antibiotics ◽  
Glycopeptide Antibiotic ◽  
Content Type ◽  
Specialized Metabolites ◽  
Chemically Modified ◽  
Induction Of Resistance ◽  
Insight Into

ABSTRACT Glycopeptide antibiotics (GPAs) include clinically important drugs used for the treatment of infections caused by Gram-positive pathogens. These antibiotics are specialized metabolites produced by several genera of actinomycete bacteria. While many GPAs are highly chemically modified, A47934 is a relatively unadorned GPA lacking sugar or acyl modifications, common to other members of the class, but which is chlorinated at three distinct sites. The biosynthesis of A47934 is encoded by a 68-kb gene cluster in Streptomyces toyocaensis NRRL 15009. The cluster includes all necessary genes for the synthesis of A47934, including two predicted halogenase genes, staI and staK. In this study, we report that only one of the halogenase genes, staI, is necessary and essential for A47934 biosynthesis. Chlorination of the A47934 scaffold is important for antibiotic activity, as assessed by binding affinity for the target N-acyl-d-Ala-d-Ala. Surprisingly, chlorination is also vital to avoid activation of enterococcal and Streptomyces VanB-type GPA resistance through induction of resistance genes. Phenotypic assays showed stronger induction of GPA resistance by the dechlorinated compared to the chlorinated GPA. Correspondingly, the relative expression of the enterococcal vanA resistance gene was shown to be increased by the dechlorinated compared to the chlorinated compound. These results provide insight into the biosynthesis of GPAs and the biological function of GPA chlorination for this medically important class of antibiotic.

scholarly journals Novel insight into theacrine metabolism revealed by transcriptome analysis in bitter tea (Kucha, Camellia sinensis)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Songlin Wang ◽  
Jiedan Chen ◽  
Jianqiang Ma ◽  
Jiqiang Jin ◽  
Liang Chen ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Transcriptome Analysis ◽  
Insight Into

scholarly journals Metabolite signatures of diverse Camellia sinensis tea populations

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaomin Yu ◽  
Jiajing Xiao ◽  
Si Chen ◽  
Yuan Yu ◽  
Jianqiang Ma ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Tea Plant ◽  
Nucleotide Polymorphisms ◽  
Phylogenetic Groups ◽  
High Accumulation ◽  
Single Nucleotide ◽  
Specialized Metabolites ◽  
Metabolomic Data ◽  
Negative Ionization ◽  
The Relationship

Abstract The tea plant (Camellia sinensis) presents an excellent system to study evolution and diversification of the numerous classes, types and variable contents of specialized metabolites. Here, we investigate the relationship among C. sinensis phylogenetic groups and specialized metabolites using transcriptomic and metabolomic data on the fresh leaves collected from 136 representative tea accessions in China. We obtain 925,854 high-quality single-nucleotide polymorphisms (SNPs) enabling the refined grouping of the sampled tea accessions into five major clades. Untargeted metabolomic analyses detect 129 and 199 annotated metabolites that are differentially accumulated in different tea groups in positive and negative ionization modes, respectively. Each phylogenetic group contains signature metabolites. In particular, CSA tea accessions are featured with high accumulation of diverse classes of flavonoid compounds, such as flavanols, flavonol mono-/di-glycosides, proanthocyanidin dimers, and phenolic acids. Our results provide insights into the genetic and metabolite diversity and are useful for accelerated tea plant breeding.

scholarly journals Haplotype-resolved genome assembly provides insights into evolutionary history of the tea plant Camellia sinensis

2021 ◽  
Author(s):  
Xingtan Zhang ◽  
Shuai Chen ◽  
Longqing Shi ◽  
Daping Gong ◽  
Shengcheng Zhang ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Evolutionary History ◽  
Green Revolution ◽  
Genomic Analysis ◽  
Tea Plant ◽  
Specific Expression ◽  
History Of ◽  
Allele Specific ◽  
Tea Cultivar

AbstractTea is an important global beverage crop and is largely clonally propagated. Despite previous studies on the species, its genetic and evolutionary history deserves further research. Here, we present a haplotype-resolved assembly of an Oolong tea cultivar, Tieguanyin. Analysis of allele-specific expression suggests a potential mechanism in response to mutation load during long-term clonal propagation. Population genomic analysis using 190 Camellia accessions uncovered independent evolutionary histories and parallel domestication in two widely cultivated varieties, var. sinensis and var. assamica. It also revealed extensive intra- and interspecific introgressions contributing to genetic diversity in modern cultivars. Strong signatures of selection were associated with biosynthetic and metabolic pathways that contribute to flavor characteristics as well as genes likely involved in the Green Revolution in the tea industry. Our results offer genetic and molecular insights into the evolutionary history of Camellia sinensis and provide genomic resources to further facilitate gene editing to enhance desirable traits in tea crops.

scholarly journals Insights into Tissue-specific Specialized Metabolism in Tieguanyin Tea Cultivar by Untargeted Metabolomics

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1817 ◽  
Author(s):  
Si Chen ◽  
Jun Lin ◽  
Huihui Liu ◽  
Zhihong Gong ◽  
Xiaxia Wang ◽  
...  
Keyword(s):  
Multivariate Analyses ◽  
Hydroxycinnamic Acid ◽  
Plant Tissues ◽  
Tissue Specific ◽  
Specialized Metabolism ◽  
Specialized Metabolites ◽  
Hydroxycinnamic Acid Amides ◽  
Tea Plants ◽  
Tea Cultivar ◽  
Different Tissues

Tea plants produce extremely diverse and abundant specialized metabolites, the types and levels of which are developmentally and environmentally regulated. However, little is known about how developmental cues affect the synthesis of many of these molecules. In this study, we conducted a comparative profiling of specialized metabolites from six different tissues in a premium oolong tea cultivar, Tieguanyin, which is gaining worldwide popularity due to its uniquely rich flavors and health benefits. UPLC-QTOF MS combined with multivariate analyses tentatively identified 68 metabolites belonging to 11 metabolite classes, which exhibited sharp variations among tissues. Several metabolite classes, such as flavonoids, alkaloids, and hydroxycinnamic acid amides were detected predominantly in certain plant tissues. In particular, tricoumaroyl spermidine and dicoumaroyl putrescine were discovered as unique tea flower metabolites. This study offers novel insights into tissue-specific specialized metabolism in Tieguanyin, which provides a good reference point to explore gene-metabolite relationships in this cultivar.

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