scholarly journals Bolting reduces ferulic acid and flavonoid biosynthesis and induces root lignification in Angelica sinensis

2022 ◽  
Vol 170 ◽  
pp. 171-179
Author(s):  
Meiling Li ◽  
Xiuwen Cui ◽  
Ling Jin ◽  
Mengfei Li ◽  
Jianhe Wei
Keyword(s):  
Ferulic Acid ◽  
Flavonoid Biosynthesis ◽  
Angelica Sinensis

scholarly journals Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Mechanism of Flavonoid Biosynthesis in Two Cultivars of Angelica sinensis

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 306
Author(s):  
Tiantian Zhu ◽  
Minghui Zhang ◽  
Hongyan Su ◽  
Meiling Li ◽  
Yuanyuan Wang ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Candidate Genes ◽  
Flavonoid Biosynthesis ◽  
Transcriptomic Analysis ◽  
Expression Level ◽  
Bioactive Metabolites ◽  
Angelica Sinensis ◽  
Yield And Quality ◽  
Flavonoid Biosynthetic Pathway ◽  
Differential Mechanism

Angelica sinensis is a traditional Chinese medicinal plant that has been primarily used as a blood tonic. It largely relies on its bioactive metabolites, which include ferulic acid, volatile oils, polysaccharides and flavonoids. In order to improve the yield and quality of A. sinensis, the two cultivars Mingui 1 (M1), with a purple stem, and Mingui 2 (M2), with a green stem, have been selected in the field. Although a higher root yield and ferulic acid content in M1 than M2 has been observed, the differences of flavonoid biosynthesis and stem-color formation are still limited. In this study, the contents of flavonoids and anthocyanins were determined by spectrophotometer, the differences of flavonoids and transcripts in M1 and M2 were conducted by metabolomic and transcriptomic analysis, and the expression level of candidate genes was validated by qRT-PCR. The results showed that the contents of flavonoids and anthocyanins were 1.5- and 2.6-fold greater in M1 than M2, respectively. A total of 26 differentially accumulated flavonoids (DAFs) with 19 up-regulated (UR) and seven down-regulated (DR) were obtained from the 131 identified flavonoids (e.g., flavonols, flavonoid, isoflavones, and anthocyanins) in M1 vs. M2. A total 2210 differentially expressed genes (DEGs) were obtained from the 34,528 full-length isoforms in M1 vs. M2, and 29 DEGs with 24 UR and 5 DR were identified to be involved in flavonoid biosynthesis, with 25 genes (e.g., CHS1, CHI3, F3H, DFR, ANS, CYPs and UGTs) mapped on the flavonoid biosynthetic pathway and four genes (e.g., RL1, RL6, MYB90 and MYB114) belonging to transcription factors. The differential accumulation level of flavonoids is coherent with the expression level of candidate genes. Finally, the network of DAFs regulated by DEGs was proposed. These findings will provide references for flavonoid production and cultivars selection of A. sinensis.

Comprehensive Analysis of Transcriptomics and Metabolomics between the Heads and Tails of Angelica Sinensis: Genes Related to Phenylpropanoid Biosynthesis Pathway

2020 ◽  
Vol 23 ◽  
Author(s):  
Jie Yang ◽  
Chi Zhang ◽  
Wei-Hong Li ◽  
Tian-Er Zhang ◽  
Guang-Zhong Fan ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Metabolic Regulation ◽  
Comprehensive Analysis ◽  
Angelica Sinensis ◽  
Rna Seq ◽  
Targeted Metabolomics ◽  
Kegg Pathways ◽  
Qrt Pcr ◽  
Phenylpropanoid Biosynthesis ◽  
Combined Strategy

Background:: In Traditional Chinese Medicine (TCM), the heads and tails of Angelica sinensis (Oliv.) Diels (AS) is used in treating different diseases due to their different pharmaceutical efficacies. The underline mechanisms, however, have not been fully explored. Objective:: Novel mechanisms responsible for the discrepant activities between AS heads and tails were explored by a combined strategy of transcriptomes and metabolomics. Method:: Six pairs of the heads and tails of AS roots were collected in Min County, China. Total RNA and metabolites, which were used for RNA-seq and untargeted metabolomics analysis, were respectively isolated from each AS sample (0.1 g) by Trizol and methanol reagent. Subsequently, differentially expressed genes (DEGs) and discrepant pharmaceutical metabolites were identified for comparing AS heads and tails. Key DEGs and metabolites were quantified by qRT-PCR and targeted metabolomics experiment. Results:: Comprehensive analysis of transcriptomes and metabolomics results suggested that five KEGG pathways with significant differences included 57 DEGs. Especially, fourteen DEGs and six key metabolites were relation to the metabolic regulation of Phenylpropanoid biosynthesis (PB) pathway. Results of qRT-PCR and targeted metabolomics indicated that higher levels of expression of crucial genes in PB pathway, such as PAL, CAD, COMT and peroxidase in the tail of AS were positively correlated with levels of ferulic acid-related metabolites. The average content of ferulic acid in tails (569.58162.39 nmol/g) was higher than those in the heads (168.73  67.30 nmol/g) (P˂0.01); Caffeic acid in tails (3.82  0.88 nmol/g) vs heads (1.37  0.41 nmol/g) (P˂0.01), and Cinnamic acid in tails (0.24  0.09 nmol/g) vs heads (0.14  0.02 nmol/g) (P˂0.05). Conclusion:: Our work demonstrated that overexpressed genes and accumulated metabolites derived from PB pathway might be responsible for the discrepant pharmaceutical efficacies between AS heads and tails.

scholarly journals Angelica sinensis (Oliv.) Diels: Influence of Value Chain on Quality Criteria and Marker Compounds Ferulic Acid and Z-Ligustilide

Medicines ◽  
2017 ◽  
Vol 4 (1) ◽  
pp. 14 ◽  
Author(s):  
Nino Giacomelli ◽  
Yang Yongping ◽  
Franz Huber ◽  
Anita Ankli ◽  
Caroline Weckerle
Keyword(s):  
Ferulic Acid ◽  
Value Chain ◽  
Quality Criteria ◽  
Angelica Sinensis ◽  
Marker Compounds

scholarly journals Ultrasensitive Detection of Ferulic Acid Using Poly(diallyldimethylammonium chloride) Functionalized Graphene-Based Electrochemical Sensor

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Lin-jie Liu ◽  
Xia Gao ◽  
Pei Zhang ◽  
Shi-lan Feng ◽  
Fang-di Hu ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Supporting Electrolyte ◽  
Differential Pulse ◽  
Quantitative Detection ◽  
Angelica Sinensis ◽  
Functionalized Graphene ◽  
Long Term Stability ◽  
Diallyldimethylammonium Chloride ◽  
Potential Alternative ◽  
Electrochemical Redox

The electrochemical redox of ferulic acid (FA) was investigated systematically by cyclic voltammetry (CV) with a poly(diallyldimethylammonium chloride) functionalized graphene-modified glassy carbon electrode (PDDA-G/GCE) as a working electrode. A simple and sensitive differential pulse voltammetry (DPV) technique was proposed for the direct quantitative determination of FA inAngelica sinensisand spiked human urine samples for the first time. The dependence of the intensities of currents and potentials on nature of the supporting electrolyte, pH, scan rate, and concentration was investigated. Under optimal conditions, the proposed sensor exhibited excellent electrochemical sensitivity to FA, and the oxidation peak current was proportional to FA concentration in the range of8.95×10-8 M~5.29×10-5 M, with a relatively low detection limit of4.42×10-8 M. This fabricated sensor also displayed acceptable reproducibility, long-term stability, and high selectivity with negligible interferences from common interfering species. Besides, it was applied to detect FA inAngelica sinensisand biological samples with satisfactory results, making it a potential alternative tool for the quantitative detection of FA in pharmaceutical analysis.

scholarly journals Assay of free ferulic acid and total ferulic acid for quality assessment of Angelica sinensis

2005 ◽  
Vol 1068 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Guang-Hua Lu ◽  
Kelvin Chan ◽  
Kelvin Leung ◽  
Chi-Leung Chan ◽  
Zhong-Zhen Zhao ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Quality Assessment ◽  
Angelica Sinensis

Extraction of ferulic acid from Angelica sinensis with supercritical CO2

2006 ◽  
Vol 20 (9) ◽  
pp. 835-841 ◽  
Author(s):  
Yongyue Sun ◽  
Shufen Li ◽  
Huiting Song ◽  
Songjiang Tian
Keyword(s):  
Ferulic Acid ◽  
Supercritical Co2 ◽  
Angelica Sinensis

scholarly journals Ferulic Acid, anAngelica sinensis-Derived Polyphenol, Slows the Progression of Membranous Nephropathy in a Mouse Model

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Chao-Wen Cheng ◽  
Wen-Liang Chang ◽  
Li-Cheng Chang ◽  
Chia-Chao Wu ◽  
Yuh-Feng Lin ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Mouse Model ◽  
Ferulic Acid ◽  
Membranous Nephropathy ◽  
Angelica Sinensis ◽  
Therapeutic Effects ◽  
Th2 Response ◽  
Adequate Treatment ◽  
Oxidative Stresses ◽  
Antiangiogenic Factors

Membranous nephropathy (MN) is a leading cause of adult nephrotic syndrome but lacks adequate treatment. Different extracts ofAngelica sinensis(AS) and one of its active compounds, ferulic acid (FA), were used to evaluate the therapeutic effects in a MN mouse model. The MN model was grouped into three subgroups: no treatment (N-T), treatment at induction of MN (Pre-T), and treatment after full-blown MN (Post-T). The results showed that the methanol (ME) layer of AS extract exhibited a therapeutic effect on MN-induced proteinuria. The ME layer-enriched compound, FA, improved the hypoalbuminemia, hyperlipidemia, and proteinuria in both Pre-T and Post-T groups. Ferulic acid also reduced the formation of oxidative protein products and increased the synthesis of antioxidant enzymes in groups Pre-T and Post-T. Regarding angiogenesis factors, the antiangiogenic factors in renal glomeruli were increased in group N-T, but, after FA treatment, only one of the antiangiogenic factors, thrombospondin-1, showed a significant decrease. Furthermore, the expression of Th2 predominant showed significant decrease in both Pre-T and Post-T groups when compared to that of N-T group. In summary, FA retarded the progression of MN, and the mechanisms involved the regulation of oxidative stresses, angiogenic and antiangiogenic factors, and attenuation of Th2 response.

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