Phenylethanol as a quorum sensing molecule to promote biofilm formation of the antagonistic yeast Debaryomyces nepalensis for the control of black spot rot on jujube

2022 ◽  
Vol 185 ◽  
pp. 111788
Author(s):  
Xingmeng Lei ◽  
Bing Deng ◽  
Changqing Ruan ◽  
Lili Deng ◽  
Kaifang Zeng
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Black Spot ◽  
Antagonistic Yeast ◽  
Quorum Sensing Molecule

scholarly journals Inhibition of Candida albicans Biofilm Formation by Farnesol, a Quorum-Sensing Molecule

2002 ◽  
Vol 68 (11) ◽  
pp. 5459-5463 ◽  
Author(s):  
Gordon Ramage ◽  
Stephen P. Saville ◽  
Brian L. Wickes ◽  
José L. López-Ribot
Keyword(s):  
Candida Albicans ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Northern Blot Analysis ◽  
Adherent Cell ◽  
Naturally Occurring ◽  
Biofilm Development ◽  
Further Development ◽  
Quorum Sensing Molecule

ABSTRACT Farnesol is a quorum-sensing molecule that inhibits filamentation in Candida albicans. Both filamentation and quorum sensing are deemed to be important factors in C. albicans biofilm development. Here we examined the effect of farnesol on C. albicans biofilm formation. C. albicans adherent cell populations (after 0, 1, 2, and 4 h of adherence) and preformed biofilms (24 h) were treated with various concentrations of farnesol (0, 3, 30, and 300 μM) and incubated at 37°C for 24 h. The extent and characteristics of biofilm formation were then assessed microscopically and with a semiquantitative colorimetric technique based on the use of 2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide. The results indicated that the effect of farnesol was dependent on the concentration of this compound and the initial adherence time, and preincubation with 300 μM farnesol completely inhibited biofilm formation. Supernatant media recovered from mature biofilms inhibited the ability of planktonic C. albicans to form filaments, indicating that a morphogenetic autoregulatory compound is produced in situ in biofilms. Northern blot analysis of RNA extracted from cells in biofilms indicated that the levels of expression of HWP1, encoding a hypha-specific wall protein, were decreased in farnesol-treated biofilms compared to the levels in controls. Our results indicate that farnesol acts as a naturally occurring quorum-sensing molecule which inhibits biofilm formation, and we discuss its potential for further development and use as a novel therapeutic agent.

scholarly journals Enterococcus faecalisV583 LuxS/AI-2 system is devoid of role in intra-species quorum-sensing but contributes to virulence in aDrosophilahost model

2018 ◽  
Author(s):  
Frederic Gaspar ◽  
Neuza Teixeira ◽  
Natalia Montero ◽  
Tamara Aleksandrzak-Piekarczyk ◽  
Renata Matos ◽  
...  
Keyword(s):  
Gene Expression ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Pathogenic Potential ◽  
Functional Roles ◽  
Injury Model ◽  
Differential Gene ◽  
Vancomycin Resistant ◽  
Quorum Sensing Molecule

AbstractThe AI-2 i nterspecies quorum-sensing molecule is produced by the LuxS enzyme and has been ascribed a role in virulence in several bacteria. The nosocomial pathogenEnterococcus faecalisinhabits several different environments where multispecies communities are established. However, despite the presence of aluxSgene in this pathogen, its role inE. faecalispathogenesis has never been assessed. In the present work, we deleted theluxSgene from the vancomycin-resistant clinical isolateE. faecalisV583 and demonstrated the lack of AI-2 production by the mutant strain. Using microarrays and externally added (S)-4,5-dihydroxy-2,3-pentanedione we showed that AI-2 is not sensed byE. faecalisas a canonical quorum-sensing molecule and that theluxSmutation caused pleiotropic effects in gene expression, which could not be complemented by extracellularly added AI-2. These global differences in gene expression affected several gene functional roles, mainly those enrolled in metabolism and transport. Metabolic phenotypi ng of theluxSmutant, using Biolog plates, showed differences in utilization of galactose. AI-2 production by LuxS was shown to be irrelevant for some phenotypes related to the pathogenic potential ofE. faecalisnamely biofilm formation, adhesion to Caco-2 cells, resistance to oxidative stress and survival inside J-774 macrophages. However, theluxSmutant was attenuated when tested in theDrosophilaseptic injury model, as its deletion led to delayed fly death. Overall our findings show that differential gene expression related to theluxSmutation cannot be ascribed to quorum-sensing. Moreover, the role of LuxS appears to be limited to metabolism.

Effect of Farnesol (A Quorum Sensing Molecule) on Biofilm Formation by Candida Species = تأثير الفارنيسول (جزئ استشعار النصاب) على تكوين الفيلم الحيوي بواسطة أنواع المبيضات المختلفة

2015 ◽  
Vol 66 (4-6) ◽  
pp. 413-418
Author(s):  
أميرة إسماعيل عبد الحميد ◽  
سناء محمد إبراهيم زكي ◽  
علا إبراهيم أحمد ◽  
مروي سعد محمد فتحي ◽  
نانسي محمد أبو شادي
Keyword(s):  
Quorum Sensing ◽  
Candida Species ◽  
Biofilm Formation ◽  
Quorum Sensing Molecule

Determination of the efficacy of azithromycin on biofilm-forming uropathogenic Escherichia coli isolated from urinary tract infection samples

2021 ◽  
Vol 30 (1) ◽  
pp. 1-11
Author(s):  
Sunjukta Ahsan ◽  
Nur E Alam ◽  
Ahmed Salman Sirajee
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Quorum Sensing ◽  
Tract Infection ◽  
Biofilm Formation ◽  
Cell Number ◽  
Planktonic Cells ◽  
Form Complex ◽  
E Coli ◽  
Quorum Sensing Molecule

The ability of E. coli to form complex surface-associated communities, called biofilms, contribute to its resistance and persistence in Urinary Tract Infection (UTI). In the present study, the ability of uropathogenic E. coli (n=30) isolated from UTI patients to form biofilm and the concentration of Azithromycin to inhibit or kill planktonic and biofilm phase bacteria were investigated. Effects of sub-Minimum Inhibitory Concentration (MIC) of Azithromycin on biofilm formation was investigated. An attempt was also taken to identify the quorum sensing molecule released by E. coli in biofilm. Among the 30 isolates, 6 (20%) were very strong, 8 (26.67%) were strong, 13 (43.33%) were moderate and 3 (10%) were weak biofilm producers. MIC and MBC (Minimum bactericidal concentration) of planktonic phase bacteria were determined and compared with MRC (Minimum regrowth concentration) and MBEC (Minimum biofilm eradication concentration) of biofilm population. MIC values ranged between 0.5 μg/ml to greater than 512 μg/ml. MBC values were measured for ten isolates and the range found was between 8-64 μg/ml. It was found that MRC values were 2-256 times greater than MIC values and MBEC values were 16-256 times greater than corresponding MBC values. After subjecting sub MIC Azithromycin levels to 10 selected isolates, 6 lost the ability to form curli fimbriae and 2 lost their capability of motility in planktonic stage, but none of this ability was lost in biofilm stage. Viable cell count of planktonic cells incubated in sub MIC Azithromycin concentration showed significant decrease of cell number whereas the number of planktonic cells without antibiotic increased significantly. In contrast, for biofilm forming cells, no change in cell number was seen with progressing time for either the presence or absence of antibiotic indicating that biofilm formation could not be inhibited by sub MIC concentrations of Azithromycin. It can be implied that Azithromycin is not the proper drug of choice for eradication of biofilm formed by E. coli. The present study failed to identify the quorum sensing molecule released by the test isolates by the method employed. Dhaka Univ. J. Biol. Sci. 30(1): 1-11, 2021 (January)

Quorum Sensing – A Stratagem for Conquering Multi-Drug Resistant Pathogens

2020 ◽  
Vol 26 ◽  
Author(s):  
Madison Tonkin ◽  
Shama Khan ◽  
Mohmmad Younus Wani ◽  
Aijaz Ahmad
Keyword(s):  
Drug Resistance ◽  
Quorum Sensing ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Cell Communication ◽  
Natural Compounds ◽  
Quorum Sensing Inhibitors ◽  
Communication Technique ◽  
Multicellular Organisms ◽  
Chemical Strategies

: Quorum sensing is defined as cell to cell communication between microorganisms, which enables microorganisms to behave as multicellular organisms. Quorum sensing enables many collaborative benefits such as synchronisation of virulence factors and biofilm formation. Both quorum sensing as well as biofilm formation encourage the development of drug resistance in microorganisms. Biofilm formation and quorum sensing are causally linked to each other and play role in the pathogenesis of microorganisms. With the increasing drug resistance against the available antibiotics and antifungal medications, scientists are combining different options to develop new strategies. Such strategies rely on the inhibition of the communication and virulence factors rather than on killing or inhibiting the growth of the microorganisms. This review encompasses the communication technique used by microorganisms, how microorganism resistance is linked to quorum sensing and various chemical strategies to combat quorum sensing and thereby drug resistance. Several compounds have been identified as quorum sensing inhibitors and are known to be effective in reducing resistance as they do not kill the pathogens but rather disrupt their communication. Natural compounds have been identified as anti-quorum sensing agents. However, natural compounds present several related disadvantages. Therefore, the need for the development of synthetic or semi-synthetic compounds has arisen. This review argues that anti-quorum sensing compounds are effective in disrupting quorum sensing and could therefore be effective in reducing microorganism drug resistance.

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