White-Rot Fungi pretreatment of lignocellulosic biomass for anaerobic digestion: Impact of glucose supplementation

Anaerobic digestion of olive-mill wastewater (OMW) was carried out in a continuously fed mode bioreactor. The bioreactor was operated at different hydraulic retention times (HRTs), using OMW, either raw or pre-treated with white-rot fungi. Two different kinds of feed were tested in this process, one which was thermally treated and subjected to sedimentation, in order to remove the solids contained and the other without any physicochemical treatment (raw OMW). Thermally pretreated OMW did not allow a stable operation even at an HRT of 30d. Further pretreatment of the OMW with a white-rot fungus for removal of the contained phenolics, allowed a stable operation at an HRT of 30 d. On the other hand, simple dilution of the raw wastewater, without any solids removal, lead to a stable operation at an HRT of 30d and was accompanied by higher production of biogas. The presence of the solids in the OMW proved to be a determining factor for the stability of the process and could be attributed to a possible adsorption on the solids of hydrophobic compounds, such as long-chain fatty acids that are toxic to methanogens.

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PENGARUH PERLAKUAN PENDAHULUAN DENGAN KULTUR CAMPURAN JAMUR PELAPUK PUTIH Phanerochaete crysosporium, Pleurotus ostreatus DAN Trametes versicolor TERHADAP KADAR LIGNIN DAN SELULOSA BAGAS

JURNAL SELULOSA ◽

10.25269/jsel.v1i02.23 ◽

2016 ◽

Vol 1 (02) ◽

Author(s):

Sita Heris Anita ◽

Euis Hermiati ◽

Raden Permana Budi Laksana

Keyword(s):

Lignocellulosic Biomass ◽

Pleurotus Ostreatus ◽

Trametes Versicolor ◽

Lignin Degradation ◽

White Rot Fungi ◽

White Rot ◽

Cellulose Content ◽

Biological Pretreatment ◽

Physico Chemical ◽

Before And After

Conversion of lignocellulosic biomass requires pretreatment in order to separate lignin from cellulose. Several methods have been introduced for pretreatment process of lignocellulosic biomass. These are physical, physico-chemical, chemical, as well as biological pretreatment. The following investigation aimed at a biological pretreatment of bagasse using mix-culture of white-rot fungi with the objective to investigate pretreatment effect on the lignin degradation of bagasse with minimum loss of cellulose content. The white-rot fungi involved Phanerochaete crysosporium (Pc), Pleurotus ostreatus (Po), and Trametes versicolor (Tv) on degrading lignin of bagasse with minimal losses of cellulose content 5% (w/v). The culture variation are as follows Po dan Tv (1:1), Po dan Pc (1:1), Pc dan Tv (1:1) and Po, Pc dan Tv (1:1:1) they were inoculated into steamed bagasse and incubated for 1, 2, 3, and 4 weeks respectively. Chemical analysis was done to determine the weight loss, lignin, and alphacellulose content before and after pretreatment. Optimum lignin degradation was achieved after two weeks incubation for each mix-culture. The most effective lignin degradation of mix-culture Po, Pc and Tv (25,26±1,57%) with minimum cellulose loss (17,27±3,76%).Key words : pretreatment, white-rot fungi, mix-culture, baggaseINTISARIKonversi biomassa lignoselulosa membutuhkan proses perlakuan pendahuluan yang bertujuan untuk memisahkan lignin dari selulosa. Beberapa metode telah banyak diketahui untuk proses perlakuan pendahuluan biomassa lignoselulosa, diantaranya perlakuan pendahuluan secara fisika, fisika-kimia, kimia dan biologi. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan kultur campur jamur pelapuk putih Phanerochaete crysosporium (Pc), Pleurotus ostreatus (Po), dan Trametes versicolor (Tv) terhadap penurunan kadar lignin bagas dengan kehilangan minimal α-selulosa. Inokulum yang digunakan sebesar 5% (w/v) dengan variasi kultur Po dan Tv (1:1), Po dan Pc (1:1), Pc dan Tv (1:1) dan Po, Pc dan Tv (1:1:1). Kultur campur jamur diinokulasikan ke dalam bagas dan diinkubasi masing-masing selama 1, 2, 3, dan 4 minggu. Analisa kimia dilakukan untuk mengetahui kehilangan berat, kadar lignin serta selulosa bagas sebelum dan setelah perlakuan pendahuluan. Hasil penelitian menunjukkan bahwa penurunan optimal kadar lignin bagas dengan kehilangan minimal α-selulosa terjadi pada waktu inkubasi dua minggu untuk setiap variasi kultur campur. Dari empat variasi kultur campur pada waktu inkubasi dua minggu menunjukkan bahwa kehilangan rata-rata lignin bagas tertinggi (325,26±1,57%) dengan kehilangan minimal α-selulosa (17,27±3,76%) terjadi pada kultur campur Pc dan Tv.Kata kunci : perlakuan pendahuluan, jamur pelapuk putih, kultur campur, bagas

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Novel sterilization method combining food preservative use and low temperature steaming for treatment of lignocellulosic biomass with white rot fungi

Industrial Crops and Products ◽

10.1016/j.indcrop.2020.112765 ◽

2020 ◽

Vol 155 ◽

pp. 112765

Author(s):

Chizuru Sasaki ◽

Sayuri Yamanaka

Keyword(s):

Low Temperature ◽

Lignocellulosic Biomass ◽

White Rot Fungi ◽

White Rot ◽

Food Preservative ◽

Sterilization Method

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Coupling structural characterization with secretomic analysis reveals mechanism of the disruption of cross-linked structure in bamboo culms by Echinodontium taxodii

10.21203/rs.2.18571/v1 ◽

2019 ◽

Author(s):

Wen Kong ◽

Jialong Zhang ◽

Qiuyun Xiao ◽

Jiashu Liu ◽

Zhixiang Cao ◽

...

Keyword(s):

Lignocellulosic Biomass ◽

Extracellular Enzymes ◽

Early Stage ◽

White Rot Fungi ◽

White Rot ◽

Biomass Recalcitrance ◽

Lignocellulose Conversion ◽

Fungal Pretreatment ◽

Insight Into

Abstract Background: Overcoming the biomass recalcitrance is essential for efficient utilization of lignocellulosic biomass in industrial bio-refining. White-rot fungi can overcome the biomass recalcitrance and accelerate the conversion of lignocellulose to biofuels via a large number of special extracellular lignocellulolytic enzymes. Previous studies try to dissect the function of extracellular enzymes on biomass resistant cross-linked structures by secretome analysis, but the bio-alteration of cross-linked structures is ignored usually. A deeper and detailed understanding of relationship between secretome and bio-alteration of cross-linked structure in lignocellulosic biomass is still lack. Results: As an efficient wood-decaying fungus, Echinodontium taxodii could improve the conversion efficiency of lignocellulose to biofuels. This study coupled comparative analysis of fungal secretomes and 2D HSQC NMR analysis of lignocellulose fractions, aiming to elucidate the role of extracellular enzymes from Echinodontium taxodii 2538 in the disruption of resistant cross-linked structure of bamboo culms. Carboxylesterases, alcohol oxidases and Class-II peroxidases showed importance in the cleavage of cross-linked structures, including ester and ether linkages of lignin-carbohydrate complexes (LCCs) and inter-unit linkages of lignin, which contributed to biomass resistance removal and cellulose exposure during the early stage of fungal decay. Moreover, the rapid oxidation of Cα-OH was found to contribute to the lignin bio-depolymerization. Conclusions: These findings revealed the detailed mechanisms of biomass recalcitrance reduction by fungal pretreatment, and provide insight into efficient strategy of lignocellulose conversion. It will advance the development in design of enzyme cocktail for efficient lignocellulose bio-refinery.

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Screening of ligninolytic fungi for biological pretreatment of lignocellulosic biomass

Canadian Journal of Microbiology ◽

10.1139/cjm-2015-0156 ◽

2015 ◽

Vol 61 (10) ◽

pp. 745-752 ◽

Cited By ~ 5

Author(s):

Chunyan Xu ◽

Deepak Singh ◽

Kathleen M. Dorgan ◽

Xiaoyu Zhang ◽

Shulin Chen

Keyword(s):

Lignocellulosic Biomass ◽

Peroxidase Activity ◽

Low Cost ◽

White Rot Fungi ◽

High Potential ◽

White Rot ◽

Barley Straw ◽

Laccase Production ◽

Biological Pretreatment ◽

Ligninolytic Fungi

To identify white rot fungi with high potential in biological pretreatment of lignocellulosic biomass, preliminary screening was carried out on plates by testing different strains for their ability to oxidize guaiacol and decolorize the dyes azure B and Poly R-478. Of the 86 strains screened, 16 were selected for secondary screening for their ligninolytic ability; however, low manganese peroxidase activity and no lignin peroxidase activity were detected. Strain BBEL0970 proved to be the most efficient in laccase production and was subsequently identified as Trametes versicolor by analysis of the ribosomal DNA internal transcribed spacer gene sequence. In combining laccase production with biological pretreatment, the replacement of glucose with barley straw significantly improved the laccase activity by up to 10.3 U/mL, which provided evidence toward potential utilization of barley straw in laccase production by BBEL0970. Simultaneously, comparison by thermogravimetric analysis of the untreated and pretreated barley straw in liquid fermentation of laccase also demonstrated the high potential of BBEL0970 in biological pretreatment of lignocellulosic biomass. This work sheds light on further exploration on the integrated process of low-cost laccase production and efficient biological pretreatment of barley straw by T. versicolor BBEL0970.

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Improvement of anaerobic degradation by white-rot fungi pretreatment of lignocellulosic biomass: A review

Renewable and Sustainable Energy Reviews ◽

10.1016/j.rser.2015.12.317 ◽

2016 ◽

Vol 59 ◽

pp. 179-198 ◽

Cited By ~ 122

Author(s):

E. Rouches ◽

I. Herpoël-Gimbert ◽

J.P. Steyer ◽

H. Carrere

Keyword(s):

Lignocellulosic Biomass ◽

Anaerobic Degradation ◽

White Rot Fungi ◽

White Rot

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Evaluation of the capacity of laccase secretion of four novel isolated white-rot fungal strains in submerged fermentation with lignocellulosic biomass

BioResources ◽

10.15376/biores.16.4.6706-6722 ◽

2021 ◽

Vol 16 (4) ◽

pp. 6706-6722

Author(s):

Qi An ◽

Wen-Yi Shi ◽

Yi-Xuan He ◽

Wen-Yao Hao ◽

Kai-Yue Ma ◽

...

Keyword(s):

Lignocellulosic Biomass ◽

Industrial Application ◽

Submerged Fermentation ◽

Enzyme Production ◽

Laccase Activity ◽

White Rot Fungi ◽

White Rot ◽

Pleurotus Pulmonarius ◽

Toona Sinensis ◽

Sorghum Straw

The capacity of novel isolated white-rot fungi secreting laccase was evaluated for various kinds of lignocellulosic biomass in submerged fermentation. The laccase secreted by Neofomitella fumosipora Han 386 and Pleurotus pulmonarius Han 527 was significantly faster than that by Coriolopsis trogii Han 751 and Coriolopsis sanguinaria An 282. Maximum laccase from N. fumosipora Han 386 on the four kinds of lignocellulosic biomass tested appeared on the first day. This phenomenon indicated that N. fumosipora Han 386 secreted laccase rapidly compared with other tested strains in this study and showed the superiority in the rate of secreting laccase. Based on the maximum laccase activity, the ability of secreting laccase of C. sanguinaria An 282 was superior to other tested novel isolated strains. On the whole, N. fumosipora Han 386 and P. pulmonarius Han 527 preferred Toona sinensis to produce laccase, C. trogii Han 751 preferred to produce laccase on Populus beijingensis, and C. sanguinaria An 282 grown on Sorghum straw was more suitable for secreting laccase. The results will be helpful for developing bioprocesses using various kinds of lignocellulosic biomass for lignocellulolytic enzyme production and enlarging the number of laccase producing strains for industrial application.

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