Comparing the effect of rooster semen extender supplemented with gamma-oryzanol and its nano form on post-thaw sperm quality and fertility

The cryopreservation process adversely affects sperm function and quality traits, causing some changes at biochemical and structural levels, due to mechanical, thermal, osmotic, and oxidative damage. Supplementation with curcumin nanoparticles could prevent and even revert this effect and could enhance the post/thawed sperm quality in the rabbit. The study amid to explore the effect of curcumin (CU) and curcumin nanoparticles (CUNPs) supplementation in semen extender on post/thawed rabbit sperm quality. Twelve fertile, healthy rabbit bucks were included, and the ejaculates were collected using artificial vaginas. Rabbit pooled semen was cryopreserved in tris-yolk fructose (TYF) extender without any supplement (control group) or extender supplemented with CU at levels of 0.5, 1 or 1.5 µg/mL (CU0.5, CU1.0, and CU1.5, respectively) or CUNPs at levels of 0.5, 1, 1.5 (CUNPs0.5, CUNPs1.0, and CUNPs1.5, respectively) and was packed in straws (0.25 mL) and stored in liquid nitrogen (−196 °C). Results revealed that CUNPs1.5 had a positive influence (p < 0.05) on post-thawing sperm progressive motility, viability, and membrane integrity as compared with the other groups. Percentages of dead sperm, abnormalities, early apoptotic, apoptotic, and necrotic sperm cells reduced (p < 0.05) in CUNPs1.5 as compared to other treatments. Using 1.5 µg/mL of CUNPs significantly improved total antioxidant capacity (TAC), GPx, while MDA and POC reduced (p < 0.05) in CU1.5 in comparison with other groups. SOD values were enhanced (p < 0.05) in CUNPs1.0 and CUNPs1.5 in relation with other treatments. Conclusively, the addition of curcumin and its nanoparticles to the extender can improve the post-thawed quality of rabbit sperm via redox signaling and reduce the apoptosis process.

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Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa

Polish Journal of Veterinary Sciences ◽

10.2478/v10181-010-0019-y ◽

2010 ◽

Vol 13 (4) ◽

pp. 571-579 ◽

Cited By ~ 5

Author(s):

W. Kordan ◽

M. Lecewicz ◽

R. Strzeżek ◽

A. Dziekońska ◽

L. Fraser

Keyword(s):

Plasma Membrane ◽

Sperm Motility ◽

Mitochondrial Function ◽

Sperm Quality ◽

Membrane Integrity ◽

Platelet Activating Factor ◽

Computer Assisted ◽

Atp Content ◽

Plasma Membrane Integrity ◽

Semen Extender

Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 × 10-3M, 1 × 10-4M, 1 × 10-5M and 1 × 10-6M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved semen treated without PAF was used as the control. Sperm quality was evaluated for motility (computer-assisted semen analysis, CASA), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (SYBR-14/PI assay and Hoechst 33258). Also, ATP content of spermatozoa was determined using a bioluminescence assay. Treatment of cryopreserved semen with 1 × 10-3 M PAF at 120 min of incubation resulted in significantly higher total sperm motility compared with the control. It was observed that PAF-improved total sperm motility was concurrent with enhanced sperm motility patterns after treatment of cryopreserved semen. Treatment of cryopreserved semen with PAF did not improve either sperm mitochondrial function or plasma membrane integrity, as monitored by different fluorescent membrane markers. Furthermore, ATP content of cryopreserved spermatozoa was significantly higher when PAF was used at a concentration of 1 × 10-3 M compared with the control and other PAF treatments, regardless of the incubation time. The findings of this study indicated that treatment with 1 × 10-3 M PAF at 120 min of incubation rendered better quality of cryopreserved canine semen, which was associated with improved sperm motility parameters and ATP content. It can be suggested that exogenous PAF addition is beneficial as a supplement for canine semen extender used for.

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Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation

Veterinary Medicine International ◽

10.4061/2010/659862 ◽

2010 ◽

Vol 2010 ◽

pp. 1-7 ◽

Cited By ~ 21

Author(s):

J. M. Morrell ◽

A. Johannisson ◽

L. Juntilla ◽

K. Rytty ◽

L. Bäckgren ◽

...

Keyword(s):

Strong Correlation ◽

Sperm Quality ◽

Sperm Concentration ◽

Single Layer ◽

Sperm Viability ◽

Flow Cytometric ◽

New Instrument ◽

Counting Chamber ◽

Semen Extender

On-stud assessment of stallion sperm quality can be problematic. A new instrument, the Nucleocounter SP-100, was validated for measuring stallion sperm concentration and viability. It was subsequently used to evaluate sperm viability in Kenney's extender and INRA96. There was a strong correlation between sperm concentrations measured by the Nucleocounter SP-100 and by the Bürker counting chamber (; ). Similarly, there was a good correlation between sperm viability results from the Nucleocounter SP-100 and flow cytometric results (; ). Sperm viability at 24 hours was significantly better for samples extended in INRA96 than in Kenney's extender (). Furthermore, sperm kinematics were better for stored samples in INRA96 than in Kenney's extender. Single Layer Centrifugation selected spermatozoa that maintained their viability better during storage for 24 hours than the uncentrifuged samples. In conclusion, the type of semen extender used and Single Layer Centrifugation were found to influence both the kinematics and viability of stallion spermatozoa. The Nucelocounter-SP100 was considered to be a useful instrument for rapidly measuring stallion sperm concentration and viability.

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Impact of Zeolite Addition in Semen Extender on Rabbit Sperm Quality after Cryopreservation

Journal of Animal and Poultry Production ◽

10.21608/jappmu.2019.63457 ◽

2019 ◽

Vol 10 (10) ◽

pp. 317-322

Author(s):

A. Mohammed ◽

W. Khalil ◽

Sh. Gabr ◽

M. Hammad ◽

Hanan Youssef ◽

...

Keyword(s):

Sperm Quality ◽

Semen Extender

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Antimicrobial Resistance in Equine Reproduction

Animals ◽

10.3390/ani11113035 ◽

2021 ◽

Vol 11 (11) ◽

pp. 3035

Author(s):

Pongpreecha Malaluang ◽

Elin Wilén ◽

Johanna Lindahl ◽

Ingrid Hansson ◽

Jane M. Morrell

Keyword(s):

Antimicrobial Resistance ◽

Bacterial Infections ◽

Detrimental Effect ◽

Antimicrobial Agents ◽

Reproductive Tract ◽

Sperm Quality ◽

The United States ◽

Critical Control Points ◽

Background Exposure ◽

Semen Extender

Bacteria develop resistance to antibiotics following low-level “background” exposure to antimicrobial agents as well as from exposure at therapeutic levels during treatment for bacterial infections. In this review, we look specifically at antimicrobial resistance (AMR) in the equine reproductive tract and its possible origin, focusing particularly on antibiotics in semen extenders used in preparing semen doses for artificial insemination. Our review of the literature indicated that AMR in the equine uterus and vagina were reported worldwide in the last 20 years, in locations as diverse as Europe, India, and the United States. Bacteria colonizing the mucosa of the reproductive tract are transferred to semen during collection; further contamination of the semen may occur during processing, despite strict attention to hygiene at critical control points. These bacteria compete with spermatozoa for nutrients in the semen extender, producing metabolic byproducts and toxins that have a detrimental effect on sperm quality. Potential pathogens such as Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa may occasionally cause fertility issues in inseminated mares. Antibiotics are added during semen processing, according to legislation, to impede the growth of these microorganisms but may have a detrimental effect on sperm quality, depending on the antimicrobial agent and concentration used. However, this addition of antibiotics is counter to current recommendations on the prudent use of antibiotics, which recommend that antibiotics should be used only for therapeutic purposes and after establishing bacterial sensitivity. There is some evidence of resistance among bacteria found in semen samples. Potential alternatives to the addition of antibiotics are considered, especially physical removal separation of spermatozoa from bacteria. Suggestions for further research with colloid centrifugation are provided.

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Cysteine addition on short-term cooled boar semen preservation and its relationship with swine field fertility

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2011001300005 ◽

2011 ◽

Vol 31 (suppl 1) ◽

pp. 25-32 ◽

Cited By ~ 4

Author(s):

Carolina K Severo ◽

Gabriel R Pereira ◽

Andressa M Pereira ◽

Gustavo F Ilha ◽

João Francisco C. de Oliveira ◽

...

Keyword(s):

Plasma Membrane ◽

Artificial Insemination ◽

Sperm Quality ◽

Treated Group ◽

Swine Industry ◽

Acrosomal Membrane ◽

Semen Preservation ◽

Boar Semen ◽

Semen Extender ◽

High Concentrations

Artificial insemination is routinely used in the swine industry to reduce the costs of production through to increase the efficiency of the refrigerated boar semen process. The objective of this study was to evaluate the effect of different levels of cysteine (CYS) added to the Beltsville Thawing Solution (BTS) extender semen during cooling for up to 72 hours. Ejaculated from three boars were collected with the gloved-hand technique and semen aliquots were diluted in BTS as follow: BTS only (BTS), BTS + 0.1mM cysteine (CYS0.1), BTS + 0.5mM cysteine (CYS0.5), BTS + 1.0mM cysteine (CYS1.0), BTS + 2.5mM cysteine (CYS2.5), BTS + 5.0mM cysteine (CYS5.0), BTS + 10.0mM cysteine (CYS10.0), and BTS + 20.0mM cysteine (CYS20.0). Evaluation of sperm integrity were analyzed using 0.5mg/ml propidium iodide (plasma membrane), 100µg/ml isothiocynate-conjugated Pisum sativun agglutinin (acrosomal membrane) and 153µM 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl carbocyanine iodide (mitochondria potential) after semen dilution at specific times (0, 24, 48 and 72 hours). Additionally, we also evaluated the effects of 5.0 mM CYS addition in the BTS extender on the maintenance of sperm quality and their influence on fertility in the swine production. After artificial insemination, animals were evaluated based on the estrous return and the number of piglet's born. Cysteine at concentrations of 10.0 and 20.0mM resulted in more pronounced reductions even at the time zero. Semen viability decreased to levels below 10% at these high levels of CYS in the first 24 hour of storage at 17ºC. At the end of the storage time, less than 65% of sperm cells had intact plasma membrane in all groups. The sperm viability decreased significantly when the semen was added at high concentrations of CYS (time "0"; CYS10.0 and CYS20.0; p<0.05), when compared to the other CYS concentrations. The BTS (10.20±0.39) treated group showed a lower rate of estrus return when compared to other (BTSCYS; 86.05±039), and it showed also the highest total number of piglets borne per treatment (12.71±3.38 vs. 9.00±3.38, respectively). In conclusion, the addition of CYS in the BTS semen extender did not maintain spermatic viability of boar cooled spermatozoa and it results in a higher percentage of return to estrus and lower number of piglets borne.

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Metformin Improves Quality of Post-Thaw Canine Semen

Animals ◽

10.3390/ani10020287 ◽

2020 ◽

Vol 10 (2) ◽

pp. 287 ◽

Cited By ~ 2

Author(s):

Jérémy Grandhaye ◽

Agnieszka Partyka ◽

Zuzanna Ligocka ◽

Agata Dudek ◽

Wojciech Niżański ◽

...

Keyword(s):

Oxidative Stress ◽

Sperm Quality ◽

Membrane Integrity ◽

Fertilization Rate ◽

Mitochondrial Activity ◽

Assisted Reproductive Technique ◽

Dna Damages ◽

Semen Extender ◽

Metabolic Activities

Sperm cryopreservation is an assisted reproductive technique routinely used in canine species for genetic conservation. However, during cryopreservation, the DNA damages are still elevated, limiting the fertilization rate. The present study was conducted to evaluate whether supplementation of canine semen extender with a molecule limiting the metabolic activities can improve the quality of frozen-thawed canine spermatozoa. We used metformin, known to limit the mitochondrial respiratory and limit the oxidative stress. Before and during the freezing procedure, metformin (50µM and 500µM) has been added to the extender. After thawing, sperm exposed to metformin conserved the same viability without alteration in the membrane integrity or acrosome reaction. Interestingly, 50µM metformin improved the sperm motility in comparison to the control, subsequently increasing mitochondrial activity and NAD+ content. In addition, the oxidative stress level was reduced in sperm treated with metformin improving the sperm quality as measured by a different molecular marker. In conclusion, we have shown that metformin is able to improve the quality of frozen-thawed dog semen when it is used during the cryopreservative procedure.

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Effects of Eugenol and Vitamin E as a Supplement to Semen Extender on Chilled Canine Sperm Quality

Advances in Animal and Veterinary Sciences ◽

10.17582/journal.aavs/2021/9.7.964.970 ◽

2021 ◽

Vol 9 (7) ◽

Author(s):

Nguyen Van Vui ◽

Nguyen Thi Kim Quyen ◽

Nguyen Thuy Linh ◽

Nguyen Thi Mong Nhi

Keyword(s):

Vitamin E ◽

Sperm Quality ◽

Semen Extender

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Effects of Dietary Supplementation of Conjugated Linoleic Acids and Their Inclusion in Semen Extenders on Bovine Sperm Quality

Animals ◽

10.3390/ani11020483 ◽

2021 ◽

Vol 11 (2) ◽

pp. 483

Author(s):

Mohammed S. Liman ◽

Vittoria Franco ◽

Claudia L. Cardoso ◽

Valentina Longobardi ◽

Bianca Gasparrini ◽

...

Keyword(s):

Seminal Plasma ◽

Semen Quality ◽

Sperm Quality ◽

Sperm Concentration ◽

Conjugated Linoleic Acids ◽

Factor I ◽

Bovine Sperm ◽

Igf I ◽

Semen Extender ◽

Oxidative Species

Isomers of conjugated linoleic acid (CLA) enhances circulating insulin-like growth factor I (IGF-I) levels. Furthermore, fertility rate of breeding bulls is positively correlated to seminal plasma IGF-I concentration. Our objective was to evaluate the effect of dietary CLA supplementation and inclusion to the semen extender on bovine semen quality and freezability. Fourteen bulls, randomly assigned to control (CTL) and CLA (50 g/day) groups, were supplemented for 10 weeks. Samples were collected at Weeks −2 (before supplementation), 0, 4, 6 (during supplementation), 10, and 11 (after supplementation). Blood and seminal plasma were analyzed for IGF-I; the ejaculates were frozen in the following subgroups: CTL (no addition to semen extender), CLA c9, t11 (50 µM), CLA c9, t11 (100 µM), CLA t10, c12 (50 µM), CLA t10, c12 (100 µM), and CLA mix (50 µM each of CLA c9, t11 and CLA t10, c12). Sperm motility, morphology, viability, mitochondrial membrane potential, and reactive oxidative species were assessed. CLA supplementation decreased ejaculates’ total volume, increased sperm concentration, beat cross frequency, and decreased oxidative stress; it also increased plasma and seminal plasma IGF-I levels compared to the CTL. The inclusion of CLA c9, t11 100 µM and CLA mixture in the extender increased live spermatozoa percentage post-thawing compared to other groups. Our results show a beneficial effect of CLA supplementation on semen quality; however, further studies evaluating fertilization rates are necessary to corroborate the results.

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