Evaluation of the effect of gamma and microwave irradiation and high temperature on the antioxidant properties of the Avicennia marina leaf extract

Author(s):  
Elahe Arbidar ◽  
Mohammad Ali Sahari ◽  
Habibollah Mirzaei ◽  
Mahdi Kashaninejad ◽  
Mohammad Molaee
2017 ◽  
Vol 13 (2) ◽  
pp. 131-136
Author(s):  
Mohammad Molaee ◽  
Mohammad Ali Sahari ◽  
Reza Esmaeilzadeh Kenari ◽  
Shiva Amirkaveei ◽  
Elahe Arbidar

2020 ◽  
Vol 16 (8) ◽  
pp. 1245-1251
Author(s):  
Mohammad Molaee ◽  
Mohammad A. Sahari ◽  
Reza E. Kenari ◽  
Shiva Amirkaveei ◽  
Elahe Arbidar

Background: Despite the widespread use of antioxidants during the storage of oils, oxidation of fats and fatty foods is still reported to be the main reason for their quality drop. Synthetic antioxidants such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) completely escape and easily decompose at high temperatures. There are serious problems in relation to the toxicity of BHA, BHT and tertiary butylhydroquinone (TBHQ), possibly due to their metabolism and accumulation in organs and tissues. So finding a useful natural antioxidant is of great importance, and due to the considerable presence of mangrove plant on the Southern Coasts of Iran, this study aims to investigate the antioxidant properties of Avicennia marina leaves. Objective: Evaluation of the antioxidant activity of the Avicennia marina leaf extract and its comparison with synthetic antioxidant TBHQ. Methods: By adding the ethanolic extracts of Avicennia marina leaves at different concentrations (2, 4 and 6 mg/mL) into soybean oil system at room temperature during 20 days and at different intervals, their peroxide (PV), thiobarbituric acid (TBA), conjugated dienes (CD) and conjugated trienes (CT), as well as fatty acid profile were measured. Antioxidant activity of the samples was compared with that of TBHQ sample. Data analysis was performed using SAS, and a comparison of average data was carried out using least significant difference (LSD) at a level of 0.01. Results: The antioxidant activity of 2 and 4 mg/mL concentrations of Avicennia marina leaf extract in soybean oil was comparable with that of 0.2 mg/mL concentration of synthetic TBHQ. As a result, this natural antioxidant can be used for the preservation of soybean oil. Conclusion: Due to the presence of phenols in the leaves, Avicennia marina leaf extract can be used as a rich source of natural antioxidants.


2009 ◽  
Vol 6 (2) ◽  
pp. 227-231 ◽  
Author(s):  
S. A. Adesegun ◽  
A. Fajana ◽  
C. I. Orabueze ◽  
H. A. B. Coker

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.


2016 ◽  
Vol 4 (1) ◽  
pp. 74
Author(s):  
Usunomena Usunobun ◽  
Gabriel Anyanwu

Background: Liver disease and toxicity is common, especially with many drug treatments. The effect of Vernonia amygdalina ethanolic leaf extract post-treatment on rats pre-treated with dimethylnitrosamine (DMN) was evaluated.Methods: Male wistar rats were orally administered DMN (single dose of 25 mg/kg) on first day and thereafter post-treated with 100 and 200mg/kg ethanolic leaf extract of Vernonia amygdalina for seven days. Analysis of serum concentrations of albumin, total protein, total bilirubin and glucose were carried out.Results: Administration of DMN alone to rats significantly increased glucose and total bilirubin concentration (P < 0.05) in the serum while it significantly reduced (P<0.05) serum total protein and albumin concentrations when compared with controls. However, post-treatment of DMN administered rats with 100 and 200 mg/kg ethanolic extract leaf of Vernonia amygdalina significantly (P<0.05) reversed these changes in a concentration dependent manner.Conclusion: In conclusion ethanolic leaf extract of Vernonia amygdalina have a potent hypoglycaemic and hepatoprotective action against dimethylnitrosamine-induced liver damage in rats may be due to its antioxidant properties.


2020 ◽  
Vol 8 (2) ◽  
pp. 74-82
Author(s):  
Forough Kajbaf ◽  
Shahrbanoo Oryan ◽  
Ramesh Ahmadi ◽  
Akram Eidi

Background: Growing evidence has shown that the apoptosis of cells plays an important role in the advancement of the Diabetic nephropathy (DN). Objectives: This study attempted to discover the therapeutic potential of Peganum harmala leaf extract in the apoptosis of diabetic kidney disease. Methods: In the present experimental research, 32 male Wistar rats were studied, and diabetes was induced by streptozotocin (STZ) (65 mg/kg). The animals were randomly divided into four groups (n=8, in each group) as follows: control, diabetic, control+leaf extract, diabetic+leaf extract. For our purposes, the methanolic extract of P. harmala leaves (150 mg/kg) was given by gavage for 28 days. Flow cytometry and real-time polymerase chain reaction (PCR) analyses were utilized to determine the percentages of apoptotic cells. Also, histological alterations and blood biochemical parameters were evaluated. Results: The P. harmala leaf extract has a high amount of flavonoids (25.84%), a lower percentage of alkaloids (0.14%), and some antioxidant properties. Serum urea (P<0.001) and apoptosis (P<0.05) significantly elevated in diabetic rats relative to the control ones. The mean of fasting blood creatinine, urea, and albumin level was not significantly changed in diabetic+leaf extract rats as compared to the diabetic ones. Histopathological results also displayed that diabetic complications in the kidney could not be improved following treatment by the leaf extract of P. harmala. In addition, the leaf extract could not significantly reduce the apoptosis and caspase-3 expression compared to diabetics in renal cells. Conclusion: Based on our findings, the leaf extract of P. harmala is unable to inhibit apoptosis in the diabetic kidney model.


2016 ◽  
Vol 8 (3) ◽  
pp. 444 ◽  
Author(s):  
Chekuri Sudhakar ◽  
Narendar Vankudothu ◽  
Shivaprasad Panjala ◽  
Nirmala Babu Rao ◽  
Roja Rani Anupalli

The herb <em>Acalypha indica </em>which belongs to Euphorbiaceae family has multiple medicinal properties which include anti-oxidant, anti-bacterial, anti-fungal, anti-inflammatory, antiulcer, anti-helmintic, anti-cancerous, anti-venom, and neuro-protective activity. The present study was designed to evaluate the phytochemical, antimicrobial and anti-oxidant activity of <em>Acalypha indica </em>leaves extracts in different solvent extractions like methanol, hexane, ethyl acetate and petroleum ether. Fresh leaves of the plant were collected and shade dried. Dried leaves were milled to obtain powder. Powder was subjected to soxhlet extraction using solvents and extracts were successively obtained. Phytochemical analysis was conducted following standard methods.  Phytochemical analysis showed the presence of Alkaloids, Phenols, Saponins, Flavanoids and Amino acids. Leaf extract of methonal have shown the highest anti-oxidation capacity than hexane, ethyl acetate and petroleum ether. Anti-microbial activity has been performed on microbes like <em>Bacillus sps</em>, <em>E.coli,</em> <em>Psuedomonas sps</em> and <em>Streptococcus sps.</em> A highest value of zone of inhibition was found in methanol extract against <em>E.coli.</em> These results provide evidence that <em>Acalypha indica </em>leaf extract possesses vital phytochemicals, antimicrobial and antioxidant properties. Hence this plant can be studied further for drug analysis for finding potent medicines for diseases.


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