Detection of Escherichia coli O157:H7 virulence genes in isolates from beef, pork, water, human and animal species in the northwest province, South Africa: public health implications

Australia exports about 150,000 to 200,000 tons of manufacturing beef to the United States annually. Each lot is tested for Escherichia coli O157 using the N-60 sampling protocol, where 60 small pieces of surface meat from each lot of production are tested. A risk assessment of E. coli O157 illness from the consumption of hamburgers made from Australian manufacturing meat formed the basis to evaluate the effect of sample size and amount on the number of illnesses predicted. The sampling plans evaluated included no sampling (resulting in an estimated 55.2 illnesses per annum), the current N-60 plan (50.2 illnesses), N-90 (49.6 illnesses), N-120 (48.4 illnesses), and a more stringent N-60 sampling plan taking five 25-g samples from each of 12 cartons (47.4 illnesses per annum). While sampling may detect some highly contaminated lots, it does not guarantee that all such lots are removed from commerce. It is concluded that increasing the sample size or sample amount from the current N-60 plan would have a very small public health effect.

Download Full-text

Strong Association Between Shiga Toxin-Producing Escherichia coli O157 and Virulence Genes stx 2 and eae as Possible Explanation for Predominance of Serogroup O157 in Patients with Haemolytic Uraemic Syndrome

European Journal of Clinical Microbiology & Infectious Diseases ◽

10.1007/s10096-003-1025-0 ◽

2003 ◽

Vol 22 (12) ◽

pp. 726-730 ◽

Cited By ~ 60

Author(s):

D. Werber ◽

A. Fruth ◽

U. Buchholz ◽

R. Prager ◽

M. H. Kramer ◽

...

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Haemolytic Uraemic Syndrome ◽

Virulence Genes ◽

Strong Association ◽

Escherichia Coli O157

Download Full-text

Prevalence of shiga toxin-producing Escherichia coli O157, O26 and O111 in milk, meat and faeces of cattle, sheep and pigs slaughtered in Benin

Journal of Applied Biosciences ◽

10.35759/jabs.152.6 ◽

2020 ◽

Vol 152 ◽

pp. 15667-15675

Author(s):

Chakirath Folakè Arikè Salifou ◽

Cyrille Boko ◽

Isidore Houaga ◽

Raoul Agossa ◽

Isabelle Ogbankotan ◽

...

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Virulence Genes ◽

Animal Origin ◽

Escherichia Coli O157 ◽

E Coli ◽

Milk Samples ◽

Food Of Animal Origin ◽

Cattle Faeces ◽

Risk Assessment And Management

Objectives: The study aimed to search for E. coli O157 and non-O157 in milk, meat and faeces of cattle, sheep and pigs slaughtered in Cotonou. Methodology and Results: One hundred and Seventy-Five (175) samples including 25 meat, 25 faeces per species and 25 milk from cattle were analysed for E. coli O157; O26 and O111 and the virulence genes were identified by PCR. The SAS software (1998) and the bilateral Z test were used to calculate and compare the identification frequencies. E. coli O157 was identified in 4% of cattle faeces, 4% of sheep faeces, and 20% of beef and, in 20% of milk samples. E. coli O26 was identified in 12% of cattle faeces and, in 8% of beef samples. E. coli O111 was identified at frequencies of 8%, and 12% in faeces of sheep and pigs, respectively. The eae gene was detected in 4% of beef, ovine meat, milk, pig faeces and in sheep faeces. stx1 was detected in 8% of milk, and in 4% of bovine and sheep faeces. The strains possessing the gene were all of E. coli O157 with the exception of one from pig faeces identified as O111. Conclusions and application of findings: The presence of these serogroups of E. coli with virulence genes poses a real food safety problem in Benin. This study findings must be taken into account for risk assessment and management related to the consumption of food of animal origin. Keywords: Benin, E. coli O157, O26, O111, faeces, meat, milk

Download Full-text

Prevalence of Escherichia coli O157:H7 among Diarrhoeic HIV/AIDS Patients in the Eastern Cape Province-South Africa

Pakistan Journal of Biological Sciences ◽

10.3923/pjbs.2008.1066.1075 ◽

2008 ◽

Vol 11 (8) ◽

pp. 1066-1075 ◽

Cited By ~ 9

Author(s):

B.O. Abong`o ◽

M.N.B. Momba ◽

V.K. Malakate ◽

J.N. Mwambakana

Keyword(s):

Escherichia Coli ◽

South Africa ◽

Escherichia Coli O157 ◽

Eastern Cape Province ◽

Eastern Cape ◽

Aids Patients ◽

Hiv Aids

Download Full-text

Detection of virulence genes and antimicrobial resistance profiles of Escherichia coli isolates from raw milk and artisanal cheese in Southern Brazil

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n1p163 ◽

2019 ◽

Vol 40 (1) ◽

pp. 163 ◽

Cited By ~ 3

Author(s):

Leandro Parussolo ◽

Ricardo Antônio Pilegi Sfaciotte ◽

Karine Andrezza Dalmina ◽

Fernanda Danielle Melo ◽

Ubirajara Maciel Costa ◽

...

Keyword(s):

Public Health ◽

Escherichia Coli ◽

Antimicrobial Susceptibility ◽

Virulence Genes ◽

Antimicrobial Agents ◽

Raw Milk ◽

Diffusion Method ◽

Public Health Issue ◽

Southern Brazil ◽

E Coli

The serrano artisanal cheese is a typical product from South region of Brazil, which is produced by skilled cheesemakers using raw milk. The contamination of this food by Escherichia coli has a great impact on public health, since it could threat the consumers’ health. The study evaluated the presence of virulence genes, antimicrobial susceptibility profiles and bofilm-production ability of Escherichia coli isolates obtained from raw milk and artisanal cheese produced in Southern Brazil. A total of 117 isolates of E. coli were characterized by multiplex PCR to detect the following virulence genes: eae for enteropatogenic E. coli (EPEC), lt and st for enterotoxigenic E. coli (ETEC), stx for shiga toxin-producing E. coli (STEC), stx and eae for enterohemorrhagic E. coli (EHEC), ipaH for enteroinvasive E. coli (EIEC) and aggR for enteroaggregative E. coli (EAEC). In addition, antimicrobial susceptibility profile to 22 antimicrobial agents was also performed by disk diffusion method, and we searched for extended-spectrum beta-lactamases (ESBL) and/or carbapenemase- producing isolates. Isolates that were positive for ESBL and carbapenemase were further investigated for the presence of the genes: blaTEM, blaSHV, blaOXA, blaCTX-M, for ESBL and blaOXA-48 for carbapenemase. Further, isolates had their ability to form biofilms investigated by the red Congo agar method. Virulence genes of E. coli were identified in 21.37% of the tested isolates, which were classified as EPEC (the most prevalent pathotype) and ETEC or EAEC. Ten (8.55%) of the total studied E. coli isolates revealed a multidrug-resistant profile, since they were resistant to three or more antimicrobial classes; whereas four isolates (3.42%) were classified as ESBL-producers and showed the presence of blaTEM gene. None of the isolates exhibited carbapenemase activity nor did they carry carbapenemase genes. From the total of E. coli isolates, 79 (67.52%) were considered potential biofilm producers. These results address a serious public health issue, since artisanal cheeses pose a risk to consumers’ health, since may be sources of dissemination of diarrheogenic E. coli, that can cause from subclinical to severe and fatal infections in children and adults, and also emphasize the need to improve adaptations/adjustments in the manufacturing processes of these products.

Download Full-text