Comparison of measurements of canine plasma glucose, creatinine, urea, total proteins, alanine aminotransferase, and alkaline phosphatase obtained with the APOLOWAKO and Vitros 250 analyzers

The aim research was to established specific clinical symptoms and changes of hematological and biochemical indices in the experimental bovine sarcocistoza. The results of the tests show that the experimental infestation of calves with Sarcocystis bovicanis sporocysts in dose of 200-250 thousands, disease manifests clinically but more pronounced is on the 25-30 days after infestation, which coincides with the development of schizogonic stages when appearing massively schizonts. On the 64th day (observation period), the condition of the animals improves, which corresponds to the step of introducing the sporochysts into the muscles. Have been established three periods of body temperature increase at the calves: on 5-7, 15-17 and 25-30th days after experimental infestation. At the infected calves have been identified changes in hematological and biochemical indices in blood. From the first days after infestation, a decrease in serum total proteins has been established, hypoalbuminemia - albumin content on the 30th day after infection is reduced with 27.7% compared to baseline (until infestation). The activity of alkaline phosphatase and aldolase increases about 2-times, and aspartataminotransferaza and alanine aminotransferase by- 2-3 times.

Download Full-text

Biochemical responses in mice experimentally infected with Paracoccidioides brasiliensis and treated with Canova

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132006000700006 ◽

2006 ◽

Vol 49 (6) ◽

pp. 897-903 ◽

Cited By ~ 4

Author(s):

Gisele Takahachi ◽

Márcia Luzia Ferrarezi Maluf ◽

Terezinha Inez Estivalet Svidzinski ◽

Luciene Setsuko Akimoto-Günther ◽

Márcia Rosangela Neves de Oliveira Hübler ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Biochemical Parameters ◽

Paracoccidioides Brasiliensis ◽

Total Proteins ◽

Internal Organs ◽

Biochemical Responses

The objective of this work was to evaluate biochemical parameters in Paracoccidioides brasiliensis infected mice and the effect of Canova medicine on these parameters. Mice infected with the isolate Paracoccidioides brasiliensis Pb18 and treated with Canova for 17 weeks were used. The biochemical parameters analysed were the levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and amylase, and the concentration of total proteins, albumin and globulins. The results suggested that the animals that were treated with Canova had less functional alterations in their internal organs.

Download Full-text

Hepatotoxic effects of chloroform extract of Artemisia macivera Linn in rats following intraperitoneal administration of different subchronic doses

Human & Experimental Toxicology ◽

10.1177/0960327110368693 ◽

2010 ◽

Vol 30 (1) ◽

pp. 25-33 ◽

Cited By ~ 1

Author(s):

SE Atawodi ◽

AC Ene ◽

DA Ameh

Keyword(s):

Alkaline Phosphatase ◽

Total Protein ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Intraperitoneal Administration ◽

Chloroform Extract ◽

Hepatic Tissue ◽

Albino Rats ◽

High Dose ◽

Hepatocellular Injury

The possible hepatotoxic effects of chloroform extract of Artemisia maciverae was evaluated biochemically and histologically using male Swiss albino rats, randomly assigned into four groups of 24 animals each. The groups (control, 50, 100 and 200 mg/kg body weight) were treated for 60 days and then monitored for another 30 days before sacrifice. Alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin (total and direct), total protein and albumin were assessed colorimetrically, while tissue specimens were subjected to histological examination following standard hematoxyline-eosin staining techniques. After 1 week of treatment, the extract caused statistically significant elevation in levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and bilirubin (total and direct), while there was significant (p < 0.05) decrease in the levels of serum total protein and albumin at the onset of treatment when compared with the control. These abnormalities in the levels of serum biochemical parameters were spontaneously corrected within 2 weeks of treatment. Similarly, histological assessment showed severe hepatic tissue injuries after 1 week, but these organs recovered spontaneously by the second week of treatment. The results indicate that long-term exposure to therapeutic doses of chloroform extract of A maciverae is relatively safe, but high dose exposure may result in hepatocellular injury.

Download Full-text

Haematological and serum chemistry profiles of ferrets (Mustela putorius furo)

Laboratory Animals ◽

10.1258/002367782781110241 ◽

1982 ◽

Vol 16 (2) ◽

pp. 133-137 ◽

Cited By ~ 32

Author(s):

Elsbeth J. Lee ◽

W. E. Moore ◽

H. C. Fryer ◽

H. C. Minocha

Keyword(s):

Alkaline Phosphatase ◽

Weight Gain ◽

Total Protein ◽

Alanine Aminotransferase ◽

Inorganic Phosphate ◽

Sorbitol Dehydrogenase ◽

Serum Chemistry ◽

Mustela Putorius ◽

Mustela Putorius Furo

Female, male and castrated male ferrets were studied. Weight gain plateaued at 28-weeks of age with males about 500 g heavier than females. No statistically significant differences in haematology were observed with age, but alkaline phosphatase and alanine aminotransferase levels fell while glucose increased. Haemolysis led to various changes including marked increases in total protein, albumin, inorganic phosphate and sorbitol dehydrogenase.

Download Full-text

Assessment of liver enzymes in saliva and serum of Iraqi patients with chronic periodontitis disease

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2020.14.1.584 ◽

2020 ◽

Vol 14 (1) ◽

pp. 20-24

Author(s):

Hussein SH. Ridha ◽

Zahraa H.M. Kadri

Keyword(s):

Alkaline Phosphatase ◽

Chronic Periodontitis ◽

Alanine Aminotransferase ◽

Liver Enzymes ◽

Serum Levels ◽

Aspartate Transaminase ◽

Gamma Glutamyl Transpeptidase ◽

Serum Samples ◽

Gamma Glutamyl ◽

Glutamyl Transpeptidase

Objective: The present study aimed to assess of four liver enzymes, Alanine Aminotransferase (ALT), Aspartate Transaminase (AST), Alkaline Phosphatase (ALP) and Gamma-Glutamyl Transpeptidase (GGT). Material and Methods: Based on periodontal clinical parameters, sixty four patients with chronic periodontitis (CP) and twenty four controls were enrolled in the study. Saliva and serum samples were collected and Automated Chemistry Analyzer AU 480 was employed to assess levels of enzymes. Results: Compared to healthy controls, the levels of the four enzymes were significant increased in serum of patients, especially in the severe group while in the saliva a significant increase observed only in the level of AST. Moreover, Alanine Aminotransferase (ALT), Aspartate Transaminase (AST), Alkaline Phosphatase (ALP) and Gamma-Glutamyl Transpeptidase (GGT) the levels of these enzymes in serum were significantly higher than those in saliva. Conclusion: ALT, AST, ALP and GGT serum levels are suggested to be important indicators for disease progression as well as predict the liver health.

Download Full-text

Blood Biomarkers of Recovery Efficiency in Soccer Players

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16183279 ◽

2019 ◽

Vol 16 (18) ◽

pp. 3279 ◽

Cited By ~ 5

Author(s):

Anna Nowakowska ◽

Dorota Kostrzewa-Nowak ◽

Rafał Buryta ◽

Robert Nowak

Keyword(s):

Alkaline Phosphatase ◽

Creatine Kinase ◽

Lactate Dehydrogenase ◽

Alanine Aminotransferase ◽

Soccer Players ◽

Control Group ◽

Iron Level ◽

Recovery Efficiency ◽

Lactate Dehydrogenase Activity ◽

Over Time

Physical exercise strongly affects human metabolism and causes biochemical changes. This study aimed to investigate the relationship between routine plasma biomarker levels and recovery efficiency in soccer players during an entire competitive match season. The players participating in the study were divided into a midfielder/defender group (seven midfielders and seven defenders) and a goalie/substitute group (six persons—goalkeepers and players with a short cumulative match-time). The fasting capillary blood samples were taken 17–24 h after each competitive match. The blood plasma was used to determine the creatinine, urea, alkaline phosphatase, creatine kinase, lactate dehydrogenase, aspartate and alanine aminotransferase, iron and magnesium levels of the athletes. The levels of (AST) (aspartate aminotransferase), (ALT) (alanine aminotransferase) and (Cr) creatinine were higher in the midfielder/defender group than in the control group, but only AST and Cr significantly varied over time (AST decreased, and Cr increased with time). The (LDH) (lactate dehydrogenase) activity and urea level were significantly lower in the midfielder/defender group than in the goalie/substitute group, and it significantly varied over time (LDH decreased, and urea increased with time). No differences in the (CK) creatine kinase and (ALP) alkaline phosphatase activities between the groups was found, although CK increased significantly with time in the midfielder/defender group (particularly midfielders in the spring round). In midfielders, the AST activity and the iron level were significantly lower in the spring than in the autumn round. On the contrary, ALT, CK, urea and magnesium levels were significantly higher in the spring than in autumn round. A long-term measurement of biochemical parameters in elite soccer players indicated that AST, CK, LDH and creatinine levels, when analyzed together, could constitute a useful set of markers for monitoring recovery periods.

Download Full-text

Comparison of a full-spectrum multi-analyte clinical analyser with six reference instruments using canine and feline blood samples

Veterinární Medicína ◽

10.17221/109/2016-vetmed ◽

2017 ◽

Vol 62 (No. 6) ◽

pp. 342-350

Author(s):

CS Lin ◽

GH Chiang ◽

CH Liu ◽

HC Tsai ◽

CC Yang ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Bile Acid ◽

Blood Urea Nitrogen ◽

Total Protein ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Total Bilirubin ◽

Total Bile Acid ◽

Analytical Performance ◽

Blood Samples

In this study, we report the characterisation of a novel centrifugation and spectrum-integrated veterinary clinical analyser, the AmiShieldTM, which has been developed for the multiplex measurement of biochemical, electrolyte and immunoassay parameters in a point-of-care testing environment. The aims of this study were to evaluate the analytical performance of the AmiShieldTM and to compare it with six reference instruments using clinical blood samples. Two hundred and four canine and 120 feline blood samples collected from veterinary teaching hospitals were analysed in parallel using the AmiShield and appropriate reference instruments. All results were evaluated separately for canine and feline specimens. The instrument’s analytical performance was evaluated initially for short- and long-term precision, bias, and observed total error using quality control material. This was followed by comparison of clinical specimens on the AmiShield analyser in parallel with the Vitros and Hitachi for biochemical parameters, VetScan and SNAPshot for total bile acids, and VetLyte and Biolyte for electrolytes. Overall, the AmiShield analyser’s performance met the standards of the American Society for Veterinary Clinical Pathology for total allowable error for most analytes, and can be considered suitable for use in veterinary clinical practices. Using canine samples, excellent correlation coefficients (r ≧ 0.92) were identified for 14 analytes of various categories including glucose, total protein, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, phosphorus, Na+, K+, Cl– and total bile acid, while good correlations (0.91 ≧ r ≧ 0.80) were recorded for albumin (r = 0.91). Bland-Altman difference plots also showed agreement (greater than 95% within Limits of Agreement) for glucose, total protein, albumin, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, Na+, K+, Cl– and total bile acid between AmiShield and the reference instruments. However, aspartate aminotransferase and phosphorus exhibited higher outliers, implying potential problems associated with matrix interferences such as lipemic samples, which warrant further study. This study demonstrates that the AmiShield compares favourably with standard reference instruments, and the new device generated data of high quality for most analytes in clinical canine and feline samples. The capability of reliably measuring multi-category analytes in one device using minute amounts (170 μl) of whole blood and short turn-around times (< 15 min) underlines the high potential of the device as a good alternative in-house diagnostic application.

Download Full-text

Serum biochemistry of 4-day-old ostriches (Struthio camelus)

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2008000900005 ◽

2008 ◽

Vol 28 (9) ◽

pp. 423-426 ◽

Cited By ~ 3

Author(s):

Renata L. Miranda ◽

Antonio V. Mundim ◽

Álisson S. Costa ◽

Ruyther V. Guimarães ◽

Frederico O.C. Silva

Keyword(s):

Alkaline Phosphatase ◽

Uric Acid ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Serum Biochemistry ◽

Lipoprotein Cholesterol ◽

Low Density ◽

Low Density Lipoprotein Cholesterol ◽

Total Proteins ◽

Males And Females

An analysis was made of 30 four-day-old ostriches to evaluate their protein, metabolite, mineral, and serum enzyme profiles, to correlate them with the birds' sex. The values obtained were: Total proteins 3.59±0.72g/dL, albumin 1.04±0.14g/dL, globulins 2.51±0.56g/dL, A:G ratio 0.43± 0.07, total cholesterol 615.10±101.15mg/dL, high density lipoprotein cholesterol (HDL-C) 132.72±20.33mg/dL, low density lipoprotein cholesterol (LDL-C) 454.93±90.81mg/dL, very low density lipoprotein cholesterol (VLDL-C) 27.45±9.96mg/dL, triglycerides 137.23±49.78mg/dL, uric acid 6.24±2.15mg/dL, urea 18.27±12.33mg/dL, creatinine 0.30±0.04mg/dL, total calcium 9.38± 0.76mg/dL, ionized calcium 7.17±0.64mg/dL, phosphorus 6.96±0.91mg/dL, Ca:P ratio 1.37±0.21, iron 24.74±13.02µg/dL, sodium 142.03±6.17mEq/L, chlorides 109.59± 4.99mEq/L, aspartate aminotransferase (AST) 200.67±31.42 U/L, alanine aminotransferase (ALT) 3.90±1.92 U/L, γ-glutamyltransferase (GGT) 1.18±0.73 U/L, alkaline phosphatase (ALP) 597.30± 231.36 U/L, and creatine kinase (CK) 2348.30±755.60 U/L. Males and females showed significant differences in total proteins, globulins, alkaline phosphatase, A:G ratio, and uric acid.

Download Full-text