Haematological and serum chemistry profiles of ferrets (Mustela putorius furo)

1982 ◽  
Vol 16 (2) ◽  
pp. 133-137 ◽  
Author(s):  
Elsbeth J. Lee ◽  
W. E. Moore ◽  
H. C. Fryer ◽  
H. C. Minocha
Keyword(s):  
Alkaline Phosphatase ◽  
Weight Gain ◽  
Total Protein ◽  
Alanine Aminotransferase ◽  
Inorganic Phosphate ◽  
Sorbitol Dehydrogenase ◽  
Serum Chemistry ◽  
Mustela Putorius ◽  
Mustela Putorius Furo

Female, male and castrated male ferrets were studied. Weight gain plateaued at 28-weeks of age with males about 500 g heavier than females. No statistically significant differences in haematology were observed with age, but alkaline phosphatase and alanine aminotransferase levels fell while glucose increased. Haemolysis led to various changes including marked increases in total protein, albumin, inorganic phosphate and sorbitol dehydrogenase.

Hepatotoxic effects of chloroform extract of Artemisia macivera Linn in rats following intraperitoneal administration of different subchronic doses

2010 ◽  
Vol 30 (1) ◽  
pp. 25-33 ◽  
Author(s):  
SE Atawodi ◽  
AC Ene ◽  
DA Ameh
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Intraperitoneal Administration ◽  
Chloroform Extract ◽  
Hepatic Tissue ◽  
Albino Rats ◽  
High Dose ◽  
Hepatocellular Injury

The possible hepatotoxic effects of chloroform extract of Artemisia maciverae was evaluated biochemically and histologically using male Swiss albino rats, randomly assigned into four groups of 24 animals each. The groups (control, 50, 100 and 200 mg/kg body weight) were treated for 60 days and then monitored for another 30 days before sacrifice. Alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin (total and direct), total protein and albumin were assessed colorimetrically, while tissue specimens were subjected to histological examination following standard hematoxyline-eosin staining techniques. After 1 week of treatment, the extract caused statistically significant elevation in levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and bilirubin (total and direct), while there was significant (p < 0.05) decrease in the levels of serum total protein and albumin at the onset of treatment when compared with the control. These abnormalities in the levels of serum biochemical parameters were spontaneously corrected within 2 weeks of treatment. Similarly, histological assessment showed severe hepatic tissue injuries after 1 week, but these organs recovered spontaneously by the second week of treatment. The results indicate that long-term exposure to therapeutic doses of chloroform extract of A maciverae is relatively safe, but high dose exposure may result in hepatocellular injury.

scholarly journals Comparison of a full-spectrum multi-analyte clinical analyser with six reference instruments using canine and feline blood samples

2017 ◽  
Vol 62 (No. 6) ◽  
pp. 342-350
Author(s):  
CS Lin ◽  
GH Chiang ◽  
CH Liu ◽  
HC Tsai ◽  
CC Yang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bile Acid ◽  
Blood Urea Nitrogen ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Total Bile Acid ◽  
Analytical Performance ◽  
Blood Samples

In this study, we report the characterisation of a novel centrifugation and spectrum-integrated veterinary clinical analyser, the AmiShield<sup>TM</sup>, which has been developed for the multiplex measurement of biochemical, electrolyte and immunoassay parameters in a point-of-care testing environment. The aims of this study were to evaluate the analytical performance of the AmiShield<sup>TM</sup> and to compare it with six reference instruments using clinical blood samples. Two hundred and four canine and 120 feline blood samples collected from veterinary teaching hospitals were analysed in parallel using the AmiShield and appropriate reference instruments. All results were evaluated separately for canine and feline specimens. The instrument’s analytical performance was evaluated initially for short- and long-term precision, bias, and observed total error using quality control material. This was followed by comparison of clinical specimens on the AmiShield analyser in parallel with the Vitros and Hitachi for biochemical parameters, VetScan and SNAPshot for total bile acids, and VetLyte and Biolyte for electrolytes. Overall, the AmiShield analyser’s performance met the standards of the American Society for Veterinary Clinical Pathology for total allowable error for most analytes, and can be considered suitable for use in veterinary clinical practices. Using canine samples, excellent correlation coefficients (r ≧ 0.92) were identified for 14 analytes of various categories including glucose, total protein, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, phosphorus, Na<sup>+</sup>, K<sup>+</sup>, Cl<sup>–</sup> and total bile acid, while good correlations (0.91 ≧ r ≧ 0.80) were recorded for albumin (r = 0.91). Bland-Altman difference plots also showed agreement (greater than 95% within Limits of Agreement) for glucose, total protein, albumin, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, Na<sup>+</sup>, K<sup>+</sup>, Cl<sup>–</sup> and total bile acid between AmiShield and the reference instruments. However, aspartate aminotransferase and phosphorus exhibited higher outliers, implying potential problems associated with matrix interferences such as lipemic samples, which warrant further study. This study demonstrates that the AmiShield compares favourably with standard reference instruments, and the new device generated data of high quality for most analytes in clinical canine and feline samples. The capability of reliably measuring multi-category analytes in one device using minute amounts (170 μl) of whole blood and short turn-around times (&lt; 15 min) underlines the high potential of the device as a good alternative in-house diagnostic application.

Methylmercury in Northern Pike (Esox lucius): Distribution, Elimination, and Some Biochemical Characteristics of Contaminated Fish

1972 ◽  
Vol 29 (11) ◽  
pp. 1519-1523 ◽  
Author(s):  
W. L. Lockhart ◽  
J. F. Uthe ◽  
A. R. Kenney ◽  
P. M. Mehrle
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Total Protein ◽  
Inorganic Phosphate ◽  
Lake Ontario ◽  
Northern Pike ◽  
Biochemical Characteristics ◽  
Body Tissues ◽  
Biochemical Profiles ◽  
One Year

Northern pike heavily contaminated with methylmercury were captured from Clay Lake, Ontario, and released in Heming Lake, Manitoba, an area relatively free of mercury. Mercury levels in muscle biopsy samples at the time of transfer and at subsequent recaptures indicated that only 30% was eliminated in one year. Distribution among various body tissues was essentially unchanged, those organs most heavily contaminated being lens, kidney, and liver in decreasing order. Biochemical profiles of blood serum constituents showed several differences between samples from the two lakes, especially in levels of inorganic phosphate, total protein, alkaline phosphatase, and cortisol. Serum values for transplanted fish tended toward those in the clean lake and we have concluded that biochemical profiles were sensitive to the environmental change.

scholarly journals Antioxidant and Hepatoprotective Properties of Tofu (Curdle Soymilk) against Acetaminophen-Induced Liver Damage in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Ndatsu Yakubu ◽  
Ganiyu Oboh ◽  
Amuzat Aliyu Olalekan
Keyword(s):  
Oxidative Stress ◽  
Alkaline Phosphatase ◽  
Weight Gain ◽  
Liver Damage ◽  
Total Protein ◽  
Antioxidant Properties ◽  
Basal Diet ◽  
Antioxidant Property ◽  
Albino Rats ◽  
Serum Alanine Aminotransferase

The antioxidant and hepatoprotective properties of tofu using acetaminophen to induce liver damage in albino rats were evaluated. Tofus were prepared using calcium chloride, alum, and steep water as coagulants. The polyphenols of tofu were extracted and their antioxidant properties were determined. The weight gain and feed intake of the rats were measured. The analysis of serum alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities and the concentrations of albumin, total protein, cholesterol, and bilirubin were analyzed. The result reveals that the antioxidant property of both soluble and bound polyphenolic extracts was significantly higher in all tofus, but the steep water coagulated tofu was recorded higher. Rats fed with various tofus and acetaminophen had their serum ALP, ALT, AST, and LDH activities; total cholesterol; and bilirubin levels significantly (P<0.05) reduced, and total protein and albumin concentrations increased when compared with basal diet and acetaminophen administered group. Therefore, all tofus curdled with various coagulants could be used to prevent liver damage caused by oxidative stress.

scholarly journals Epizootology and biochemical Pparameters of Dogs with babesiosis in barnaul

2020 ◽  
Vol 14 (2) ◽  
pp. 46-52
Author(s):  
N. M. Ponamarev ◽  
M. Yu. Novikova M. ◽  
N. V. Tikhaya
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Blood Cells ◽  
Alanine Aminotransferase ◽  
White Blood Cells ◽  
German Shepherd ◽  
Age Related ◽  
Calcium And Phosphorus ◽  
Conjugated Bilirubin

The purpose of the research is studying the epizootology of babesiosis and biochemical parameters of dogs with babesiosis in Barnaul. Materials and methods. The studies were conducted at the Department of Microbiology, Epizootology, Parasitology and Veterinary-Sanitary Examination of the Altai State Agrarian University and at the Barnaul Central Veterinary Hospital. Statistical analysis was performed according to the laboratory research journal from 2013 to 2017. Babesiosis was diagnosed based on clinical evidence and microscopic examination of peripheral blood smears. Alanine aminotransferase, aspartate aminotransferase, total and conjugated bilirubin, creatinine, urea, alkaline phosphatase, total protein, calcium and phosphorus contained in the blood serum was determined. In total, 1258 blood samples of dogs of different sex, age and breed were examined. In order to identify age-related features in hematological parameters, we formed 4 groups of 30 dogs in each age group. We studied indicators such as the erythrocyte sedimentation rate, the total number of red blood cells, and white blood cells, hemoglobin concentration and color index, and counted formed elements. The spread of ixodic ticks was studied in Barnaul and a suburban area of Chernitsky Forest. Ixodic ticks were collected on flag. A total of 535 live ticks of the Ixodidae family were collected. The species of ixodic ticks was identified using the Guide to arachnoentomoses. Results and discussion. The rate of babesia infection in dogs was 36.3%. Most often babesiosis occurs in outbred dogs, the terrier, German Shepherd dog, Cocker Spaniel, Central Asian Shepherd dog and dachshund. Males, especially those over the age of 8, are more infected with Babesia. Most cases of dog lesions by babesiosis occur in Zheleznodorozhny, Central and Industrial Districts of Barnaul. The disease is diagnosed from early April to late June, and from early August to late October. Significant indicators for identifying the pathology of individual systems and organs of the animal affected by babesiosis are aspartate aminotransferase, alanine aminotransferase, creatinine, urea, total and conjugated bilirubin, and alkaline phosphatase. The level of total protein under the age of two years is lowered, while calcium and phosphorus are within physiological values. 

Toxicological Interactions of Cassia Senna and Nerium oleander in the Diet of Rats

2002 ◽  
Vol 30 (04) ◽  
pp. 579-587 ◽  
Author(s):  
M. A. Al-Yahya ◽  
A. H. Al-Farham ◽  
S. E. I. Adam
Keyword(s):  
Alkaline Phosphatase ◽  
Body Weight ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Wistar Rats ◽  
Feed Utilization ◽  
Nerium Oleander ◽  
Serum Aspartate Aminotransferase ◽  
Male Wistar Rats

The toxic effects of diet containing 10% of C. senna L. fruits or 10% of N. oleander L. leaves or their 1 : 1 mixture (5%+5%) on male Wistar rats treated for 6 weeks were investigated. Diarrhea was a prominent sign of C. senna L. toxicosis. In both phytotoxicities, there were decreases in body weight gains, inefficiency of feed utilization, dullness and enterohepatonephropathy. These findings accompanied by leukopenia and anemia were correlated with alterations of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities and concentrations of total protein, albumin, urea and other serum constituents. In both phytotoxicities, the ability of the liver to excrete bilirubin remained unchanged. Feeding the mixture of C. senna L. fruits and N. oleander L. leaves caused more serious effects and death of rats. The implications of these findings are discussed.

scholarly journals Effects of feeding graded levels of whole cottonseed on haemato-biochemical parameters of red Sokoto bucks

2020 ◽  
Vol 18 (2) ◽  
pp. 83-90
Author(s):  
J.I. Itodo ◽  
P.I. Rekwot ◽  
T.T. Aluwong ◽  
J.S. Rwuaan ◽  
K.O. Abah ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Alanine Aminotransferase ◽  
Biochemical Parameters ◽  
Control Group ◽  
Transferase Activity ◽  
Albumin Concentration ◽  
Whole Cottonseed ◽  
Group D ◽  
Aspartate Transferase

Whole cottonseed (WCS) is an important source of protein for ruminants, however, it contains a polyphenolic secondary metabolite gossypol which may reduce its palatability and cause pathophysiological effects. The study was aimed at evaluating the effects of feeding graded levels of WCS on the haemato-biochemical parameters of Red Sokoto bucks (RSB). After a 14-day pre-treatment period, 20 bucks (n = 5) were assigned for 90 days to one of four isonitrogeneous treatments: control (A); 0 % (B); 15 % (C); 30 % and (D); 45 %. Blood was collected for Packed Cell Volume (PCV) and serum analyses at days 0, 45 and 90 of the experimental period for concentrations of urea, creatinine, total protein, albumin, globulin and activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP). The PCV was decreased in groups fed above 15 % of WCS on days 45. There were no significant (P > 0.05) differences in globulin concentration, alanine aminotransferase and alkaline phosphatase activities but aspartate transferase activity was higher (p < 0.05) in group D (45% WCS) compared to the various treatment groups. The urea concentration was higher (p < 0.05) in groups B and D at day 45 compared to the control group. The creatinine concentration was higher (p < 0.05) in group D at days 45 and 90 compared to the control group. The serum total protein and albumin concentration were higher (p < 0.05) in groups C and D at day 45 compared to the control group. Our finding revealed that prolonged feeding of bucks above 30 % WCS for more than 60 days exerted considerable deleterious and adverse effects on blood constituents leading to aneamia, hyper-cretenaemia, ureamia, increased levels of total protein, albumin, aspartate transferase activity and death. Keywords: Biochemical parameters, Bucks, Gossypol, Serum, Whole cotton seed

scholarly journals Influence of 8-Hour Road Transportation on Selected Physiological Parameters in Horses

2012 ◽  
Vol 56 (2) ◽  
pp. 193-197 ◽  
Author(s):  
Artur Niedźwiedź ◽  
Marcin Zawadzki ◽  
Henryk Filipowski ◽  
Józef Nicpoń
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Total Bilirubin ◽  
Physiological Responses ◽  
Venous Blood ◽  
Road Transportation ◽  
Physiological Variables

Abstract The objective of this study was to assess the physiological responses in mature healthy horses prior to an 8 h transport, as well as immediately and 24 h after transportation in a horse truck, under autumn conditions typical for central Poland. The study was conducted on a group of 60 horses of different breed. Horses ranged from 4 to 10 years of age. Venous blood was taken 3 times: I - directly before loading the horses to the trailer, II - directly after unloading the horses from the trailer, III - after period of 24 h resting. There was no change in the concentrations of urea, cholesterol, creatinine, and magnesium and in the activity of alkaline phosphatase during the three subsequent samplings. Albumin, total protein, alanine aminotransferase, aspartate aminotransferase, total bilirubin, creatine kinase, and triglycerides were mostly influenced by the transport. The obtained results indicate that the transportation of horses alters concentrations of physiological variables of their metabolism.

scholarly journals Enzyme blood profile in lactating cows of different bulls’ genetic lines

2021 ◽  
Vol 282 ◽  
pp. 03007
Author(s):  
V.I. Eremenko ◽  
Yu.I. Gatilova ◽  
A.V. Blednova ◽  
S.Yu. Steblovskaya ◽  
G.I. Shvets
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Lactic Dehydrogenase ◽  
Blood Profile ◽  
Blood Samples ◽  
Lactating Cows ◽  
Ldh Activity

Scientific and production experiments were carried out on lactating cows of the Simmental breed. 4 groups of cows derived from different lines of bulls Romulus, Redad, Huxle, and Honig were formed for the experiment. Each group contained 10 heads. The level of dairy productivity of experimental cows was the same and amounted to about 11 thousand kg of milk per lactation. The keeping conditions for the animals were the same. Feeding levels were consistent with their dairy productivity. Blood for the study was taken once a month prior to morning feeding. The following were determined in blood samples: total protein, alanine aminotransferase (ALT), aspartate aminotransferase (AAT), alkaline phosphatase (AP) and lactic dehydrogenase (LDH). During lactation, the highest level of total protein, the activity of ALT, AAT, AP, LDH was observed at the peak of lactation, followed by their decrease by the end of lactation regardless of cows’ genetic affiliation. Relatively higher levels of total protein and ALT, AAT and LDH activity have been observed in lactating cows of the bull line Romulus relative to cows of the bull lines Redad, Huxle and Honig. There were no differences within the cows’ genetic lines in the activity of alkaline phosphatase.

Age-related changes in haematology and serum chemistry of Weiser–Maples guineapigs (Cavia porcellus)

2005 ◽  
Vol 39 (3) ◽  
pp. 321-330 ◽  
Author(s):  
M Kitagaki ◽  
M Yamaguchi ◽  
M Nakamura ◽  
K Sakurada ◽  
T Suwa ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Cell ◽  
Total Cholesterol ◽  
Total Protein ◽  
Cavia Porcellus ◽  
Serum Chemistry ◽  
Male And Female ◽  
Age Related ◽  
Haematological Changes ◽  
Age Related Changes

Age-related changes in haematology and serum chemistry values were examined in male and female Weiser–Maples guineapigs ( Cavia porcellus). Haematological changes that significantly ( P < 0.01) correlated with ageing were increased white blood cell and neutrophil counts in both sexes, decreased lymphocyte counts in both sexes, decreased reticulocyte and platelet counts in males, and decreased basophil counts in females. For serum chemistry, increases in total protein, triglycerides, blood urea nitrogen and creatinine were seen in both sexes, along with increases in total cholesterol in males and sodium in females. Decreased alkaline phosphatase in both sexes and decreased chloride in males were significantly ( P < 0.01) associated with age. These age-related changes are compared with the published literature.

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