Ultrasound-mediated structural changes in cells revealed by FTIR spectroscopy: A contribution to the optimization of gene and drug delivery

2011 ◽  
Vol 84 (1) ◽  
pp. 74-85 ◽  
Author(s):  
Paola Grimaldi ◽  
Lucia Di Giambattista ◽  
Serena Giordani ◽  
Ion Udroiu ◽  
Deleana Pozzi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Ftir Spectroscopy ◽  
Structural Changes ◽  
In Cells

Structural Changes in the Schiff Base Region of Squid Rhodopsin upon Photoisomerization Studied by Low-Temperature FTIR Spectroscopy†

Biochemistry ◽  
2006 ◽  
Vol 45 (9) ◽  
pp. 2845-2851 ◽  
Author(s):  
Toru Ota ◽  
Yuji Furutani ◽  
Akihisa Terakita ◽  
Yoshinori Shichida ◽  
Hideki Kandori
Keyword(s):  
Schiff Base ◽  
Low Temperature ◽  
Ftir Spectroscopy ◽  
Structural Changes ◽  
Base Region

scholarly journals Carbon Nanomaterials Modified Biomimetic Dental Implants for Diabetic Patients

Nanomaterials ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2977
Author(s):  
Renjini Vijay ◽  
Jayanti Mendhi ◽  
Karthika Prasad ◽  
Yin Xiao ◽  
Jennifer MacLeod ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Dental Implants ◽  
Structural Changes ◽  
Carbon Nanomaterials ◽  
Antibacterial Properties ◽  
Diabetic Patients ◽  
Crucial Issue ◽  
Biomimetic Materials ◽  
Dental Clinics ◽  
Healing Phase

Dental implants are used broadly in dental clinics as the most natural-looking restoration option for replacing missing or highly diseased teeth. However, dental implant failure is a crucial issue for diabetic patients in need of dentition restoration, particularly when a lack of osseointegration and immunoregulatory incompetency occur during the healing phase, resulting in infection and fibrous encapsulation. Bio-inspired or biomimetic materials, which can mimic the characteristics of natural elements, are being investigated for use in the implant industry. This review discusses different biomimetic dental implants in terms of structural changes that enable antibacterial properties, drug delivery, immunomodulation, and osseointegration. We subsequently summarize the modification of dental implants for diabetes patients utilizing carbon nanomaterials, which have been recently found to improve the characteristics of biomimetic dental implants, including through antibacterial and anti-inflammatory capabilities, and by offering drug delivery properties that are essential for the success of dental implants.

scholarly journals Structural Changes Due to the Deprotonation of the Proton Release Group in the M-Photointermediate of Bacteriorhodopsin as Revealed by Time-Resolved FTIR Spectroscopy†

Biochemistry ◽  
2008 ◽  
Vol 47 (44) ◽  
pp. 11598-11605 ◽  
Author(s):  
Joel E. Morgan ◽  
Ahmet S. Vakkasoglu ◽  
Johan Lugtenburg ◽  
Robert B. Gennis ◽  
Akio Maeda
Keyword(s):  
Ftir Spectroscopy ◽  
Structural Changes ◽  
Proton Release ◽  
Time Resolved ◽  
Release Group

scholarly journals Molecular Structural Changes in Alfalfa Detected by ATR-FTIR Spectroscopy in Response to Silencing of TT8 and HB12 Genes

2018 ◽  
Vol 19 (4) ◽  
pp. 1046 ◽  
Author(s):  
Yaogeng Lei ◽  
Abdelali Hannoufa ◽  
David Christensen ◽  
Haitao Shi ◽  
Luciana Prates ◽  
...  
Keyword(s):  
Ftir Spectroscopy ◽  
Structural Changes

Ultrastructural Changes in Euglena After Ultraviolet Irradiation

1973 ◽  
Vol 13 (3) ◽  
pp. 799-809
Author(s):  
A. MICHAELS ◽  
A. GIBOR
Keyword(s):  
Ultraviolet Light ◽  
Ultraviolet Irradiation ◽  
Euglena Gracilis ◽  
Structural Changes ◽  
Ultrastructural Changes ◽  
Electron Microscopic ◽  
Bleaching Process ◽  
In Cells

The structural changes associated with the ultraviolet-induced bleaching of light-grown cells of Euglena gracilis were investigated. Our light- and electron-microscopic observations of the bleaching process indicate that there is a continuity of plastid structure in cells 5 generations after receiving a bleaching dose of ultraviolet light. There seems to be a continuous dilution of the plastid thylakoids and a decrease in plastid size in the bleaching cells. There also seems to be a change in the position of the plastids in relation to the mitochondria in the bleaching cells. The plastids and possibly the mitochondria are the only organelles which are affected by the ultraviolet irradiation. The continuity of plastids in bleaching cells of Euglena is discussed in relation to the proposed effect of the ultraviolet light.

scholarly journals Spray-Formed Layered Polymer Microneedles for Controlled Biphasic Drug Delivery

Polymers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 369 ◽  
Author(s):  
Seok Park ◽  
Min Kim ◽  
Seung-Ki Baek ◽  
Jung-Hwan Park ◽  
Seong-O Choi
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Diffusion Barrier ◽  
Structural Changes ◽  
Tertiary Structure ◽  
Ex Vivo ◽  
Release Kinetics ◽  
In Vitro Release ◽  
Transdermal Drug Delivery System ◽  
Initial Burst

In this study we present polymeric microneedles composed of multiple layers to control drug release kinetics. Layered microneedles were fabricated by spraying poly(lactic-co-glycolic acid) (PLGA) and polyvinylpyrrolidone (PVP) in sequence, and were characterized by mechanical testing and ex vivo skin insertion tests. The compression test demonstrated that no noticeable layer separation occurred, indicating good adhesion between PLGA and PVP layers. Histological examination confirmed that the microneedles were successfully inserted into the skin and indicated biphasic release of dyes incorporated within microneedle matrices. Structural changes of a model protein drug, bovine serum albumin (BSA), in PLGA and PVP matrices were examined by circular dichroism (CD) and fluorescence spectroscopy. The results showed that the tertiary structure of BSA was well maintained in both PLGA and PVP layers while the secondary structures were slightly changed during microneedle fabrication. In vitro release studies showed that over 60% of BSA in the PLGA layer was released within 1 h, followed by continuous slow release over the course of the experiments (7 days), while BSA in the PVP layer was completely released within 0.5 h. The initial burst of BSA from PLGA was further controlled by depositing a blank PLGA layer prior to forming the PLGA layer containing BSA. The blank PLGA layer acted as a diffusion barrier, resulting in a reduced initial burst. The formation of the PLGA diffusion barrier was visualized using confocal microscopy. Our results suggest that the spray-formed multilayer microneedles could be an attractive transdermal drug delivery system that is capable of modulating a drug release profile.

scholarly journals Magnetically responsive layer-by-layer microcapsules can be retained in cells and under flow conditions to promote local drug release without triggering ROS production

Nanoscale ◽  
2020 ◽  
Vol 12 (14) ◽  
pp. 7735-7748 ◽  
Author(s):  
Jordan E. Read ◽  
Dong Luo ◽  
Tina T. Chowdhury ◽  
Rod J. Flower ◽  
Robin N. Poston ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Migration ◽  
Drug Release ◽  
Layer By Layer ◽  
Ros Production ◽  
Flow Conditions ◽  
Drug Delivery Vehicles ◽  
Delivery Vehicles ◽  
Migration Assays ◽  
In Cells

Magnetically responsive LbL microcapsules are biologically inert, magnetically retained in flow and cell migration assays so are retainable drug delivery vehicles.

Structural changes in cells of Clostridium perfringens infected with a short-tailed bacteriophage

1971 ◽  
Vol 17 (3) ◽  
pp. 397-402 ◽  
Author(s):  
David E. Bradley ◽  
Judith F. M. Hoeniger
Keyword(s):  
Electron Microscope ◽  
Clostridium Perfringens ◽  
Structural Changes ◽  
Cell Envelope ◽  
Cell Lysis ◽  
Cell Periphery ◽  
Normal Cells ◽  
Phage Growth ◽  
Cooked Meat ◽  
In Cells

A culture of Clostridium perfringens strain 80 in cooked meat broth contained three types of cell: normal, long, and small round cells. The process of phage maturation and cell lysis was studied in all three forms using the electron microscope. In normal cells, the nucleoplasm first enlarged, then small clear areas, slightly larger than intracellular phages, appeared mainly around the cell periphery. The nucleoplasm dispersed and mature phage particles were formed. Subsequent lysis was caused by the breaking off of portions of the cell envelope leaving holes for the release of the contents. Long cells were also able to support phage growth.

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