Transcriptome sequencing and comparative analysis between male and female floral buds of the persimmon (Diospyros kaki Thunb.)

2019 ◽  
Vol 246 ◽  
pp. 987-997 ◽  
Author(s):  
Shuzhan Li ◽  
Peng Sun ◽  
Gaigai Du ◽  
Liyuan Wang ◽  
Huawei Li ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Transcriptome Sequencing ◽  
Diospyros Kaki ◽  
Male And Female ◽  
Floral Buds

scholarly journals Programmed Cell Death Facilitates the Formation of Unisexual Male and Female Flowers in Persimmon (Diospyros kaki Thunb.)

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 234
Author(s):  
Wang ◽  
Li ◽  
Suo ◽  
Han ◽  
Diao ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Quantitative Polymerase Chain Reaction ◽  
Normal Growth ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Diospyros Kaki ◽  
Male And Female ◽  
Floral Buds ◽  
Unisexual Flowers ◽  
Normal Flower

Most varieties of persimmon (Diospyros kaki Thunb.) are gynoecious, while just a few are either monoecious, androgynomonoecious, or androecious. Persimmon flowers initially contain the original androecium and gynoecium followed by arrest of either pistil or stamen primordia before maturity. Abortion of inappropriate primordium in persimmon may be related to programmed cell death (PCD). To test this hypothesis, hematoxylin and eosin (H&E) staining, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay, cyt-c immunohistochemistry (IHC) assay, transmission electron microscopy (TEM) observation, and real-time quantitative polymerase chain reaction (RT-qPCR) were used to clarify the occurrence and molecular regulatory mechanism of PCD in male and female floral buds during the 14 days prior to the second crucial morphological stage when inappropriate sexual primordia were arrested to form unisexual flowers. Accordingly, dead cells in inappropriate sex organs were largely accumulated during the microsporocyte and macrosporocyte period of male and female floral buds, respectively. This may explain the abortion of inappropriate sex organs, leading to unisexual flowers. PCD is necessary for normal growth and development in persimmons, as dead cells could also be observed in the normal flower organs. High levels of a gene homologous to AMC9 may have accelerated the arrest of the pistil primordium during differentiation, leading to male unisexual flowers, and high levels of genes homologous to MeGI, BAG5, AifA, and HSP70 in female floral buds were positively correlated with the arrest of stamen primordium. Future studies may try to transform unisexual flowers into hermaphroditic flowers by the regulation of PCD artificially, which will be helpful to the controlled pollination experiments.

Endogenous phytohormone profiles in male and female floral buds of the persimmons (Diospyros kaki Thunb.) during development

2017 ◽  
Vol 218 ◽  
pp. 213-221 ◽  
Author(s):  
Peng Sun ◽  
Jiaru Li ◽  
Gaigai Du ◽  
Weijuan Han ◽  
Jianmin Fu ◽  
...  
Keyword(s):  
Diospyros Kaki ◽  
Male And Female ◽  
Floral Buds ◽  
Endogenous Phytohormone

Transcriptome sequencing and comparative analysis of Schizochytrium mangrovei PQ6 at different cultivation times

2016 ◽  
Vol 38 (10) ◽  
pp. 1781-1789 ◽  
Author(s):  
Minh Hien Hoang ◽  
Cuong Nguyen ◽  
Huy Quang Pham ◽  
Lam Van Nguyen ◽  
Le Hoang Duc ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Transcriptome Sequencing

scholarly journals Comparative analysis of the performance of highly qualified male and female judokas following the results of the XXXII Summer Olympic Games – 2020

2021 ◽  
pp. 57-66
Author(s):  
Serhii Ilchenko ◽  
Oksana Solodka ◽  
Andrii Fedorishko ◽  
Shandor Kovach ◽  
Olena Kravchenko ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Olympic Games ◽  
Theoretical Research ◽  
Highly Qualified ◽  
Male And Female ◽  
Analytical Work ◽  
Tactical Training ◽  
Pedagogical Models ◽  
Competitive Activities

According to the results of the analysis of scientific-methodical, special and reference literature (monitoring of Internet sources) it is established, that the issue of analytical and statistical review (comparative analysis) of technical and tactical training of judokas and highly qualified judokas is devoted to the insufficient number of scientific and methodological works, which requires further research and emphasizes the relevance and practical component of the chosen area of scientific research. The main purpose of the study is to conduct a comparative analysis of the technical and tactical arsenal of highly qualified male and female judokas (champions and winners of the XXXII Summer Olympic Games 2020, Tokyo, Japan). In the process of research and analytical work, the following methods of theoretical research were used: axiomatic, idealization, historical and logical, convergence from the specific, formalization (mathematical and statistical), and so on. As a result of the study, members of the research group conducted a comparative analysis of the technical and tactical arsenal of highly qualified male and female judokas (champions and winners of the XXXII Summer Olympic Games 2020, Tokyo, Japan). In order to increase the effectiveness of competitive activities of Ukrainian athletes (men and women) who specialize in judo at different stages of their long-term training, it is advisable to focus the attention of leading coaches on the development of appropriate training programs (pedagogical models) that provide: perfect mastering by Judoists of theoretical aspects of current rules of judo; increasing the number and variety of attacking and defensive techniques; increasing the coefficient of reliability of technical and tactical actions (combinations); development of tactile sensitivity and coordination; formation of high psychophysical readiness for long-term competitive activity (high training loads), etc.

scholarly journals KEPEDULIAN LINGKUNGAN SISWA DITINJAU DARI ASPEK PENGETAHUAN TENTANG KONSEP DASAR EKOLOGI DAN GENDER

2017 ◽  
Vol 18 (02) ◽  
pp. 64-77
Author(s):  
Suhardin Suhardin
Keyword(s):  
Data Analysis ◽  
Comparative Analysis ◽  
Basic Concept ◽  
Environmental Concern ◽  
Female Students ◽  
Male Students ◽  
Environmental Concerns ◽  
Male And Female ◽  
Significant Difference ◽  
Basic Concepts

  The objective of this research is to know the diffrences of environmental concern as well as to find out the relionship between basic concept of ecology knowledge with environmental concern among  male and female students. The Comparative Analysis was conducted in SMA Depok involving 96 students chosen by using purposive random sampling.The data analysis and interpretation indicated that: 1. There is significant correlation in students basic concepts of ecology knowledge between with the environmental concerns, 2. There is verry significant difference students among male and female environmental concerns  3. There is significant correlation in male students basic concepts of ecology knowledge between with the environmental concerns, 4. There is significant correlation in female students  basic concepts of ecology knowledge between with the environmental concerns.

“I Say What I Mean,” Said Alice: An Analysis of Gendered Discourse in Physical Education

1991 ◽  
Vol 10 (2) ◽  
pp. 210-225 ◽  
Author(s):  
Jan Wright ◽  
R.C. King
Keyword(s):  
Comparative Analysis ◽  
Physical Education ◽  
Social Order ◽  
Gender Relations ◽  
Functional Model ◽  
Grammatical Structure ◽  
Teacher Language ◽  
Male And Female ◽  
Distinctive Features ◽  
Female Teachers

An analysis of the teacher language used in physical education lessons reveals the influence of many discourses that are current in our culture, including those related to gender. The subtle meanings carried in the linguistic choices made by teachers provide one framework through which girls and boys come to form particular relationships with their bodies. These relationships are culturally constructed and influence the desire to be active and the choice of activities. The process of gender production can be made visible by a comparative analysis of the lexico-grammatical structure of texts from two gymnastic lessons using the systemic functional model of linguistics developed by Michael Halliday (1978, 1985). The most distinctive features that have emerged from the analysis have been the different linguistic choices made by male and female teachers in the grammatical realization of interpersonal meanings. These differences contribute to the construction of a social order for the participants in physical education lessons that mirrors the gender relations in the culture of the larger society. Revealing the way the language works provides for the possibility of different linguistic choices—choices that may constitute a different social reality.

scholarly journals Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon

DNA Research ◽  
2020 ◽  
Vol 27 (3) ◽  
Author(s):  
Kanae Masuda ◽  
Eiji Yamamoto ◽  
Kenta Shirasawa ◽  
Noriyuki Onoue ◽  
Atsushi Kono ◽  
...  
Keyword(s):  
Genetic Factors ◽  
Diospyros Kaki ◽  
Dependent Manner ◽  
Male And Female ◽  
Association Analyses ◽  
Genome Wide ◽  
Allele Dosage ◽  
Partial Inactivation ◽  
In Cis ◽  
Segregating Population

Abstract Sexuality is one of the fundamental mechanisms that work towards maintaining genetic diversity within a species. In diploid persimmons (Diospyros spp.), separated sexuality, the presence of separate male and female individuals (dioecy), is controlled by the Y chromosome-encoded small-RNA gene, OGI. On the other hand, sexuality in hexaploid Oriental persimmon (Diospyros kaki) is more plastic, with OGI-bearing genetically male individuals, able to produce both male and female flowers (monoecy). This is thought to be linked to the partial inactivation of OGI by a retrotransposon insertion, resulting in DNA methylation of the OGI promoter region. To identify the genetic factors regulating branch sexual conversion, genome-wide correlation/association analyses were conducted using ddRAD-Seq data from an F1 segregating population, and using both quantitative and diploidized genotypes, respectively. We found that allelic ratio at the Y-chromosomal region, including OGI, was correlated with male conversion based on quantitative genotypes, suggesting that OGI can be activated in cis in a dosage-dependent manner. Genome-wide association analysis based on diploidized genotypes, normalized for the effect of OGI allele dosage, detected three fundamental loci associated with male conversion. These loci underlie candidate genes, which could potentially act epigenetically for the activation of OGI expression.

scholarly journals Database Creation and Dialect-Wise Comparative Analysis of Prosodic Features for Punjabi Language

2019 ◽  
Vol 29 (1) ◽  
pp. 1275-1282
Author(s):  
Shipra J. Arora ◽  
Rishipal Singh
Keyword(s):  
Comparative Analysis ◽  
Speech Recognition ◽  
Significant Variation ◽  
Recognition System ◽  
Speech Recognition System ◽  
Prosodic Features ◽  
Speech Corpus ◽  
Male And Female ◽  
Distinctive Features ◽  
Language Analysis

Abstract The paper represents a Punjabi corpus in the agriculture domain. There are various dialects in the Punjabi language and the main concentration is on major dialects, i.e. Majhi, Malwai and Doabi for the present study. A speech corpus of 125 isolated words is taken into consideration. These words are uttered by 100 speakers, i.e. 60 Malwi dialect speakers (30 male and 30 female), 20 Majhi dialect speakers (10 male and 10 female) and 20 Doabi dialect speakers (10 male and 10 female). Tonemes, adhak (geminated) and nasal words are selected from the corpus. Recordings have been processed through two mediums. The paper also elaborates some distinctive features of the corpus. This corpus is of quite significance for the speech recognition system. Prosodic characteristics such as intonation, rhythm and stress create a crucial impact on the speech recognition system. These characteristics vary from language to language as well as various dialects of a language. This paper portrays a comparative analysis of isolated words prosodic features of Malwi, Majhi and Doabi dialects of Punjabi language. Analysis is done using the PRAAT tool. Pitch, intensity, formant I and formant II values are extracted for toneme, adhak, nasal (bindi) and nasal (tippi) words. For all kinds of words, there is a significant variation in pitch (fundamental frequency), intensity, formant I and formant II values of male and female speakers of Malwi, Majhi and Doabi dialects. A detailed analysis has been discussed throughout this paper.

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