Lycopene β-cyclase plays a critical role in carotenoid biosynthesis during persimmon fruit development and postharvest ripening

2021 ◽  
Vol 287 ◽  
pp. 110265
Author(s):  
Yingwei Qi ◽  
Hanxiao Wu ◽  
Jia Liu ◽  
Lijuan Chen ◽  
Zitao Jiang ◽  
...  
Keyword(s):  
Fruit Development ◽  
Critical Role ◽  
Carotenoid Biosynthesis ◽  
Persimmon Fruit ◽  
Postharvest Ripening

scholarly journals Physiological and transcriptome analysis of indica rice 'MH86' overexpressing OsSPL14

2018 ◽  
Author(s):  
Ling Lian ◽  
Wei He ◽  
Qiu hua Cai ◽  
Hui Zhang ◽  
Cheng rong Ren ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Transgenic Plant ◽  
Transcriptome Analysis ◽  
Indica Rice ◽  
Critical Role ◽  
Lignin Biosynthesis ◽  
Growth Period ◽  
Carotenoid Biosynthesis ◽  
Maturity Stage ◽  
Wild Type Plant

OsSPL14, identified as IDEAL PLANT ARCHITECTURE1 (IPA1) or WEALTHY FARMER'S PANICLE (WFP) gene, plays a critical role in regulating rice plant architecture. Here, the study showed that OsSPL14-overexpression transgenic rice plants had shorter growth period, short-narrow flag leaves, and thick-green leaves. Compared with wild type plant 'MH86', transgenic plants had higher chlorophyll a (Ca), chlorophyll b (Cb) and carotenoid (Cx) content at both seedling and maturity stage. Meanwhile, transcriptome analysis identified 473 up-regulated and 103 down-regulated genes in transgenic plant. The expression of differentially expressed genes (DEGs) involved in carotenoid biosynthesis, abscisic acid (ABA) metabolism and lignin biosynthesis increased significantly. Most of DEGs participated in 'plant hormone signal transduction' and 'starch and sucrose metabolism' are also up-regulated in transgenic plant. In addition, there were higher levels of ABA and gibberellin acid (GA3) in OsSPL14-overexpression transgenic plants. Moreover, the content of culm lignin, cellulose, silicon and potassium all increased dramatically. Thus, these results demonstrate that overexpression of OsSPL14 has influence on leaf development, hormone level and culm composition in rice, which provide more insight into understanding the function of OsSPL14.

scholarly journals Transcriptome analysis of the oriental melon (Cucumis meloL. var.makuwa) during fruit development

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e2834 ◽  
Author(s):  
Ah-Young Shin ◽  
Yong-Min Kim ◽  
Namjin Koo ◽  
Su Min Lee ◽  
Seokhyeon Nahm ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Fruit Development ◽  
De Novo ◽  
Expression Patterns ◽  
Soluble Sugars ◽  
Carotenoid Biosynthesis ◽  
Northern China ◽  
Differentially Expressed ◽  
Oriental Melon ◽  
Expression Of Genes

BackgroundThe oriental melon (Cucumis meloL. var.makuwa) is one of the most important cultivated cucurbits grown widely in Korea, Japan, and northern China. It is cultivated because its fruit has a sweet aromatic flavor and is rich in soluble sugars, organic acids, minerals, and vitamins. In order to elucidate the genetic and molecular basis of the developmental changes that determine size, color, and sugar contents of the fruit, we performedde novotranscriptome sequencing to analyze the genes expressed during fruit development.ResultsWe identified a total of 47,666 of representative loci from 100,875 transcripts and functionally annotated 33,963 of the loci based on orthologs inArabidopsis thaliana. Among those loci, we identified 5,173 differentially expressed genes, which were classified into 14 clusters base on the modulation of their expression patterns. The expression patterns suggested that the differentially expressed genes were related to fruit development and maturation through diverse metabolic pathways. Analyses based on gene set enrichment and the pathways described in the Kyoto Encyclopedia of Genes and Genomes suggested that the expression of genes involved in starch and sucrose metabolism and carotenoid biosynthesis were regulated dynamically during fruit development and subsequent maturation.ConclusionOur results provide the gene expression patterns related to different stages of fruit development and maturation in the oriental melon. The expression patterns give clues about important regulatory mechanisms, especially those involving starch, sugar, and carotenoid biosynthesis, in the development of the oriental melon fruit.

scholarly journals Downstream of GA4, PbCYP78A6 Regulates Parthenogenesis by Mediating Cell Cycle-Related Genes in Pear (Pyrus Bretshneider Rehd.)

2021 ◽  
Author(s):  
Haiqi Zhang ◽  
Wei Han ◽  
Huibin Wang ◽  
Liu Cong ◽  
Rui Zhai ◽  
...  
Keyword(s):  
Fruit Development ◽  
Molecular Mechanisms ◽  
Fruit Set ◽  
Critical Role ◽  
Fruit Production ◽  
Parthenocarpic Fruit ◽  
Self Incompatibility ◽  
Horticultural Crops ◽  
Upstream Regulator ◽  
Ectopic Overexpression

Abstract Background: Parthenocarpy results in traits attractive to both consumers and breeders, and it overcomes the obstacle of self-incompatibility in the fruit set of horticultural crops, including pear (Pyrus bretshneider). However, there is limited knowledge regarding the genetic and molecular mechanisms that regulate parthenogenesis. Results: Here, in a transcriptional comparison between pollination-dependent and GA4-induced parthenocarpy, PbCYP78A6 was identified and proposed as a candidate gene involved in parthenocarpy. PbCYP78A6 is similar to Arabidopsis thaliana CYP78A6 and is highly expressed in pear hypanthia. The increased PbCYP78A6 expression, as assessed by RT-qPCR, was induced by pollination and GA4 exposure. The ectopic overexpression of PbCYP78A6 contributed to parthenocarpic fruit production in tomato. The PbCYP78A6 expression coincided with fertilized and parthenocarpic fruitlet development and the expression of fruit development-related genes as assessed by cytological observations and RT-qPCR, respectively. PbCYP78A6 RNA interference and overexpression revealed that the gene is an upstream regulator of fruit development-related genes in pear. Conclusions: Our findings indicate that PbCYP78A6 plays a critical role in cell proliferation and provide insights into controlling parthenocarpy.

scholarly journals Downstream of GA4, PbCYP78A6 participates in regulating cell cycle-related genes and parthenogenesis in pear (Pyrus bretshneideri Rehd.)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Haiqi Zhang ◽  
Wei Han ◽  
Huibin Wang ◽  
Liu Cong ◽  
Rui Zhai ◽  
...  
Keyword(s):  
Fruit Development ◽  
Molecular Mechanisms ◽  
Fruit Set ◽  
Critical Role ◽  
Fruit Production ◽  
Parthenocarpic Fruit ◽  
Self Incompatibility ◽  
Horticultural Crops ◽  
Upstream Regulator ◽  
Fruit Formation

Abstract Background Parthenocarpy results in traits attractive to both consumers and breeders, and it overcomes the obstacle of self-incompatibility in the fruit set of horticultural crops, including pear (Pyrus bretshneider). However, there is limited knowledge regarding the genetic and molecular mechanisms that regulate parthenogenesis. Results Here, in a transcriptional comparison between pollination-dependent fruit and GA4-induced parthenocarpy, PbCYP78A6 was identified and proposed as a candidate gene involved in parthenocarpy. PbCYP78A6 is similar to Arabidopsis thaliana CYP78A6 and highly expressed in pear hypanthia. The increased PbCYP78A6 expression, as assessed by RT-qPCR, was induced by pollination and GA4 exposure. The ectopic overexpression of PbCYP78A6 contributed to parthenocarpic fruit production in tomato. The PbCYP78A6 expression coincided with fertilized and parthenocarpic fruitlets development and the expression of fruit development-related genes as assessed by cytological observations and RT-qPCR, respectively. PbCYP78A6 RNA interference and overexpression in pear calli revealed that the gene is an upstream regulator of specific fruit development-related genes in pear. Conclusions Our findings indicate that PbCYP78A6 plays a critical role in fruit formation and provide insights into controlling parthenocarpy.

scholarly journals Transcriptome regulation of carotenoids in five flesh-colored watermelon (Citrullus lanatus)

2021 ◽  
Author(s):  
Pingli Yuan ◽  
Muhammad Jawad Umer ◽  
Nan He ◽  
Shengjie Zhao ◽  
Xuqiang Lu ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Candidate Genes ◽  
Differentially Expressed Genes ◽  
Fruit Development ◽  
Citrullus Lanatus ◽  
Carotenoid Biosynthesis ◽  
Differentially Expressed ◽  
Flesh Color ◽  
Hub Genes ◽  
Data Resource

Abstract Background: Fruit flesh color in watermelon (Citrullus lanatus) is a great index for evaluation of the appearance quality and a key contributor influencing consumers' preferences, but the molecular mechanism of this intricate trait remain largely unknown. Here, the carotenoids and transcriptome dynamics during the fruit development of cultivated watermelon with five different flesh colors were analyzed.Results: A total of 13 carotenoids and 16781 differentially expressed genes (DEGs) including 1295 transcription factors (TFs) were detected in five watermelon genotypes during the fruit development. The comprehensive accumulation patterns of carotenoids were closely related to flesh color. A number of potential structural genes and transcription factors were found to be associated with the carotenoid biosynthesis pathway using comparative transcriptome analysis. The differentially expressed genes were divided into six subclusters and distributed in different GO terms and metabolic pathways. Furthermore, we performed weighted gene co-expression network analysis and predicted hub genes in six main modules determining carotenoid contents. Cla018406 (a chaperone protein dnaJ-like protein) may be a candidate gene for β-carotene accumulation and highly expressed in orange flesh-colored fruit. Cla007686 (a zinc finger CCCH domain-containing protein) was highly expressed in the red flesh-colored watermelon, maybe a key regulator of lycopene accumulation. Cla003760 (membrane protein) and Cla021635 (photosystem I reaction center subunit II) were predicted to be hub genes and may play an essential role in yellow flesh formation.Conclusions: The composition and contents of carotenoid in five watermelon genotypes vary greatly. A series of candidate genes were revealed through combined analysis of metabolites and transcriptome. These results provide an important data resource for dissecting the candidate genes and molecular basis governing flesh color formation in watermelon fruit.

scholarly journals Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase

2000 ◽  
Vol 22 (6) ◽  
pp. 503-513 ◽  
Author(s):  
Luisa Maria Lois ◽  
Manuel Rodriguez-Concepcion ◽  
Francesca Gallego ◽  
Narciso Campos ◽  
Albert Boronat
Keyword(s):  
Fruit Development ◽  
Tomato Fruit ◽  
Carotenoid Biosynthesis ◽  
Regulatory Role ◽  
Phosphate Synthase

scholarly journals Transcriptome regulation of carotenoids in five flesh-colored watermelons (Citrullus lanatus)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Pingli Yuan ◽  
Muhammad Jawad Umer ◽  
Nan He ◽  
Shengjie Zhao ◽  
Xuqiang Lu ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Candidate Genes ◽  
Differentially Expressed Genes ◽  
Fruit Development ◽  
Citrullus Lanatus ◽  
Carotenoid Biosynthesis ◽  
Differentially Expressed ◽  
Flesh Color ◽  
Hub Genes ◽  
Data Resource

Abstract Background Fruit flesh color in watermelon (Citrullus lanatus) is a great index for evaluating the appearance quality and a key contributor influencing consumers’ preferences. But the molecular mechanism of this intricate trait remains largely unknown. Here, the carotenoids and transcriptome dynamics during the fruit development of cultivated watermelon with five different flesh colors were analyzed. Results A total of 13 carotenoids and 16,781 differentially expressed genes (DEGs), including 1295 transcription factors (TFs), were detected in five watermelon genotypes during the fruit development. The comprehensive accumulation patterns of carotenoids were closely related to flesh color. A number of potential structural genes and transcription factors were found to be associated with the carotenoid biosynthesis pathway using comparative transcriptome analysis. The differentially expressed genes were divided into six subclusters and distributed in different GO terms and metabolic pathways. Furthermore, we performed weighted gene co-expression network analysis and predicted the hub genes in six main modules determining carotenoid contents. Cla018406 (a chaperone protein dnaJ-like protein) may be a candidate gene for β-carotene accumulation and highly expressed in orange flesh-colored fruit. Cla007686 (a zinc finger CCCH domain-containing protein) was highly expressed in the red flesh-colored watermelon, maybe a key regulator of lycopene accumulation. Cla003760 (membrane protein) and Cla021635 (photosystem I reaction center subunit II) were predicted to be the hub genes and may play an essential role in yellow flesh formation. Conclusions The composition and contents of carotenoids in five watermelon genotypes vary greatly. A series of candidate genes were revealed through combined analysis of metabolites and transcriptome. These results provide an important data resource for dissecting candidate genes and molecular basis governing flesh color formation in watermelon fruit.

scholarly journals Overexpression of OsSPL14 results in transcriptome and physiology changes in indica rice ‘MH86’

2020 ◽  
Vol 90 (2) ◽  
pp. 265-278 ◽  
Author(s):  
Ling Lian ◽  
Huibin Xu ◽  
Hui Zhang ◽  
Wei He ◽  
Qiuhua Cai ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Indica Rice ◽  
Critical Role ◽  
Lignin Biosynthesis ◽  
Growth Period ◽  
Carotenoid Biosynthesis ◽  
Transcriptional Level ◽  
Chl A ◽  
Rice Plants ◽  
Transgenic Lines

AbstractOryza sativa SPL14 (OsSPL14), identified as the IDEAL PLANT ARCHITECTURE1 or WEALTHY FARMER’S PANICLE gene, plays a critical role in regulating rice plant architecture. Here, OsSPL14-overexpression transgenic rice plants had shorter growth periods, short narrow flag leaves, and thick green leaves compared with wild type ‘MH86’ plants (WT). Additionally, transgenic lines had higher chlorophyll a (Chl a), chlorophyll b (Chl b), and carotenoid (Car x) contents at both seedling and mature stages. Expression of OsSPL14 increased at transcriptional level, and OsSPL14 protein level was substantially increased in transgenic lines relative to WT. A transcriptome analysis identified 473 up-regulated and 103 down-regulated genes in the transgenic plants. The expression of differentially expressed genes (DEGs) involved in carotenoid biosynthesis, abscisic acid (ABA) metabolism, and lignin biosynthesis increased significantly. Most of DEGs participated in “plant hormone signal transduction” and “starch and sucrose metabolism” were also up-regulated in the transgenic plants. In addition, there were higher ABA and gibberellin acid 3 (GA3) levels in OsSPL14-overexpression rice plants at seedling and tillering stages compared with WT. In contrast with that of WT, lignin and cellulose contents of culm increased distinctly. Also, silicon and potassium contents increased dramatically in transgenic lines. Meanwhile, the chalkiness ratios and chalkiness degrees decreased, and the gel consistency levels improved in transgenic lines. Thus, overexpression of OsSPL14 influenced growth period, leaf development, hormonal levels, culm composition, and grain quality characters of rice, which provides more insight into the function of OsSPL14.

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