scholarly journals Application of a novel prioritisation strategy using non-target screening for evaluation of temporal trends (1969–2017) of contaminants of emerging concern (CECs) in archived lynx muscle tissue samples

2022 ◽  
pp. 153035
Author(s):  
Wiebke Dürig ◽  
Nikiforos A. Alygizakis ◽  
Karin Wiberg ◽  
Lutz Ahrens
Keyword(s):  
Muscle Tissue ◽  
Temporal Trends ◽  
Contaminants Of Emerging Concern ◽  
Tissue Samples ◽  
Target Screening

scholarly journals Novel prioritisation strategies for evaluation of temporal trends in archived white-tailed sea eagle muscle tissue in non-target screening

2021 ◽  
pp. 127331
Author(s):  
Wiebke Dürig ◽  
Nikiforos A. Alygizakis ◽  
Frank Menger ◽  
Oksana Golovko ◽  
Karin Wiberg ◽  
...  
Keyword(s):  
Muscle Tissue ◽  
Temporal Trends ◽  
Target Screening

scholarly journals Development of a Method to Determine Albendazole and Its Metabolites in the Muscle Tissue of Yellow Perch Using High-Performance Liquid Chromatography with Fluorescence Detection

2011 ◽  
Vol 94 (2) ◽  
pp. 446-452 ◽  
Author(s):  
Donglei Yu ◽  
Nathan Rummel ◽  
Badar Shaikh
Keyword(s):  
High Performance Liquid Chromatography ◽  
Muscle Tissue ◽  
Mobile Phase ◽  
Yellow Perch ◽  
High Performance ◽  
Ammonium Acetate ◽  
Hplc Method ◽  
Tissue Samples ◽  
Phase Combination

Abstract An HPLC method was developed for the determination of albendazole (ABZ) and its metabolites, a sulfoxide (ABZSO), a sulfone (ABZSO2), and albendazole-2-aminosulfone (ABZ-2-NH2SO2), from yellow perch muscle tissue with adhering skin. The muscle tissue samples were made alkaline with potassium carbonate and extracted with ethyl acetate, followed by a series of liquidliquid extraction steps. After solvent evaporation, the residue was reconstituted in the initial mobile phase combination of the gradient. The mobile phase consisted of a buffer, 50 mM ammonium acetate (pH 4.0) in 10 methanolwater, and 100 acetonitrile. The gradient was from 20 acetonitrile to 85 acetonitrile. The analytes were chromatographed on an RP Luna C18(2) column and detected by fluorescence with excitation and emission wavelengths of 290 and 330 nm, respectively. The average recoveries from fortified muscle tissue for ABZ (20100 ppb), ABZ-SO (20200 ppb), ABZSO2 (8100 ppb), and ABZ-2-NH2SO2 (20100 ppb) were 85, 95, 101, and 86, respectively, with corresponding CV values of 9, 3, 6, and 4, respectively. Their LOQ values were 10, 10, 1, and 10 ppb, respectively. The procedure was applied to determine ABZ and its major metabolites in the incurred muscle tissue of yellow perch obtained after orally dosing the fish with ABZ.

Indexes of Lipid Metabolism in Fish from the Zaporizke Reservoir

2017 ◽  
Vol 64 ◽  
pp. 10-16 ◽  
Author(s):  
Tamila Ananieva
Keyword(s):  
Muscle Tissue ◽  
Total Lipid ◽  
Iodine Value ◽  
Liver Tissue ◽  
Predatory Fish ◽  
Contamination Level ◽  
European Perch ◽  
Tissue Samples ◽  
Lipid Contents ◽  
Pike Perch

The total lipid contents and iodine value of fats had been determined in fish from two sections of the Zaporizke Reservoir (Ukraine) with different contamination levels. Research was conducted using the muscle and liver tissue samples from pike-perch (Sander lucioperca), european perch (Perca fluviatis), prussian carp (Carassius gibelio), roach (Rutilus rutilus) and rudd (Scardinius erythrophthalmus). Obtained data showed that at the contaminated zone, the total lipid contents were significantly (p ≤ 0.05) reduced in muscle tissue of pike-perch and european perch in comparison with the samples from “conventionally clean” lower section of the reservoir. Increased iodine value of fats in muscle tissue and liver tissue were detected in the predatory fish and both fish groups respectively. The research results could be used for estimation of the adaptation processes in freshwater fish as well as for indication of environmental contamination level in the natural and artificial reservoirs.

scholarly journals Safety Assessment of the Auto Manipulation Device for Acupuncture in Sprague-Dawley Rats: Preclinical Evaluation of the Prototype

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Geng-Hao Liu ◽  
Meng-Yen Tsai ◽  
Gwo-Jyh Chang ◽  
Chao-Min Wu ◽  
Sheng-Kai Lin ◽  
...  
Keyword(s):  
Real Time ◽  
Muscle Tissue ◽  
Safety Issue ◽  
Laboratory Data ◽  
Needle Insertion ◽  
Control Group ◽  
Sprague Dawley ◽  
Tissue Samples ◽  
Physiological Functions ◽  
Whole Process

Background. The Auto Manipulation Device for Acupuncture (AMDA) is designed for providing stable, quantified effects and higher frequency when doing lifting and thrusting manipulation. The purpose of this study is to investigate the safety of manipulation by AMDA in different frequency and duration in healthy rats. Methods. The study was divided into two parts: single intervention and once a day for a week. 12 rats and 15 rats were randomly allocated to different groups: Control (needle insertion only), AMDA (2Hz/10Mins), AMDA (2Hz/20Mins), AMDA (20Hz/10Mins), and AMDA (20Hz/20Mins) for single and repeated interventions. Real-time physiological functions, laboratory data, and the bilateral muscle tissue of acupoint (ST 36) were obtained after the intervention. Results. We found neither real-time physiological functions nor laboratory data differences between control group and AMDA groups in both parts. In the muscle tissue samples, the slight damage had been observed in the AMDA group with a frequency of 2 Hz for 20 minutes after once intervention, and the repeated session groups noted more obvious tissue damage with fibrotic change. Although the period was shorter, higher frequency manipulation caused more damage that fibroblast nuclei became more slender and obvious. However, no significant adverse effect was noted such as crippled and molting in the whole process. Conclusion. Our study suggested that the safety issue of AMDA operation in rats is feasible because there was no difference between control group and AMDA groups among real-time physiological functions and laboratory data. However, manipulation with higher frequency should be more preserved.

scholarly journals TRICHINELLOSIS OF THE STONE MARTEN (MARTES FOINA) IN CENTRAL RUSSIA

2021 ◽  
pp. 56-60
Author(s):  
Andreyanov
Keyword(s):  
Muscle Tissue ◽  
Diaphragm Muscle ◽  
Stone Marten ◽  
Martes Foina ◽  
Tissue Samples ◽  
Central Russia ◽  
Animal Muscle ◽  
Artificial Gastric Juice ◽  
Males And Females ◽  
Fur Animals

Over the past 5 years, research has been carried out in natural biocenosis on the stone marten (Martes foina) infected with the pathogen of trichinellosis in Central Russia. Biological material for research was collected in hunting farms, reserves and "green zones" of the Central Region of Russia (the Vladimir, Moscow, Nizhny Novgorod, Tula, Ryazan, Oryol and Bryansk regions) during the opening of amateur sports hunting for fur animals from 2017 to 2021. Diagnostics and isolation of helminth larvae from animal muscle tissue samples were performed by the compressor trichinelloscopy and digestion in artificial gastric juice. The degree of host invasion with helminth and invasion intensity were determined. A total of 35 animals were studied. As a result of studies, the infection rate of helminthozoonosis in animals was from 8.3 to 40.0%. The intensity of invasion was recorded between 2 and 39 larvae in 1 g of diaphragm muscle tissue. Trichinella invasion among martens was observed equally in both males and females. The largest percentage of the invasive form of the helminthiasis pathogen occurred in animals older than 2 years. The adverse situation of helminthozoonosis among fur animals was noted in the Ryazan region.

Matrix Solid Phase Dispersion Isolation and Liquid Chromatographic Determination of Sulfadimethoxine in Catfish (Ictalurus punctatus) Muscle Tissue

1990 ◽  
Vol 73 (6) ◽  
pp. 868-871 ◽  
Author(s):  
Austin R Long ◽  
Lily C Hsieh ◽  
Marsha S Malbrou ◽  
Charles R Short ◽  
Steven A Barker
Keyword(s):  
Muscle Tissue ◽  
Ictalurus Punctatus ◽  
Solid Phase ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Fish Muscle ◽  
Tissue Samples ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method for the isolation and liquid chromatographic determination of sulfadimethoxine In catfish (Ictalurus punctatus) muscle tissue Is presented. Blank control and sulf adlmethox- Ine-fortlfled fish muscle tissue samples (0.5 g) were blended with octadecylsllyl (C18,40 /μm, 18% load, endcapped) derivatlzed silica packing material. A column made from the C18/ fish tissue blend was first washed with hexane (8 mL), following which the sulfadimethoxine was eluted with dlchloromethane (8 mL). The eluant contained sulfadimethoxine analyte that was free from Interfering compounds when analyzed by liquid chromatography with UV detection (photodlode array, 270 nm). Standard curves for sulfadimethoxine Isolated from fortified samples were linear (0.999 ± 0.001) with an average relative percentage recovery of 101.1 ± 4.2% for the concentration range (50, 100, 200,400, 800, and 1600 ng/g) examined using sulfamethoxazole as the Internal standard. The interassay variability was 10.7 ± 8.2% with an Intra-assay variability of 2.2%.

Matrix Solid Phase Dispersion Isolation and Liquid Chromatographic Determination of Oxytetracycline in Catfish (Ictalurus punctatus) Muscle Tissue

1990 ◽  
Vol 73 (6) ◽  
pp. 864-867 ◽  
Author(s):  
Austin R Long ◽  
Lily C Hsiesh ◽  
Marsha S Malbrough ◽  
Charles R Short ◽  
Steven A Barker
Keyword(s):  
Muscle Tissue ◽  
Ictalurus Punctatus ◽  
Solid Phase ◽  
Chromatographic Determination ◽  
Fish Muscle ◽  
Butylated Hydroxytoluene ◽  
Tissue Samples ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method for Isolation and liquid chromatographic determination of oxytetracycllne In catfish (Ictalurus punctatus) muscle tissue Is presented. Blank control and oxytetracycllne- fortlfied fish muscle tissue samples (0.5 g) were blended with octadecylsilyl (C18, 40 μm, 18% load, endcapped) derlvatized silica packing material (2 g) containing 0.05 g each of oxalic acid and disodlum ethylenedlamlnetetraacetate. A column made from the C18/fish tissue matrix was first washed with hexane (8 mL), following which the oxytetracycllne was eluted with acetonltrile-methanol (1 + 1, v/v) containing 0.06% w/v each of butylated hydroxyanlsole and butylated hydroxytoluene. The eluate contained oxytetracycllne analyte that was free from Interfering compounds when analyzed by liquid chromatography with UV detection (photodlode array set at 365 nm). Standard curves for oxytetracycllne Isolated from fortified samples were linear (0.998 ± 0.002) with an average absolute percentage recovery of 80.9 ± 6.6% for the concentration range (50,100,200, 400, 800, 1600, and 3200 ng/g) examined. The Interassay variability was 11.3 ± 5.2% with an Intra-assay variability of 1.1%.

Attachment and Proliferation of Bacteria on Meat

1989 ◽  
Vol 52 (3) ◽  
pp. 173-177 ◽  
Author(s):  
KING-THOM CHUNG ◽  
JAMES S. DICKSON ◽  
JOHN D. GROUSE
Keyword(s):  
Staphylococcus Aureus ◽  
Muscle Tissue ◽  
Room Temperature ◽  
Fat Tissue ◽  
Tissue Samples ◽  
Initial Cell ◽  
Muscle Tissues ◽  
Significant Difference ◽  
Cell Concentrations ◽  
Number Of Cells

The attachment of bacteria (Serratia marcescens, Staphylococcus aureus, Streptococcus faecalis, Salmonella arizonae, Pseudomonas aeruginosa, and Listeria monocytogenes), to lean muscle tissue and fat tissue was investigated. The number of cells attached to the meat was directly proportional to the initial cell concentrations present. There was no significant difference in the number of cells attached between the lean muscle tissue and fat tissues among the organisms tested. All bacteria tested except P. aeruginosa proliferated better on the lean muscle tissues than on the fat tissue at ambient temperature for 72 h. No significant attachment competition to tissue samples was seen between L. monocytogenes and P. aeruginosa, however, the numbers of P. aeruginosa were greater than L. monocytogenes (after 24 h). Similarly, no competitive attachments between S. aureus and S. marcescens, S. faecalis and S. arizonae were observed; but the numbers of S. marcescens were greater than S. aureus, and S. arizonae were greater than S. faecalis, when the inoculated meat was incubated at room temperature for 24 h.

Genetic variation in carotenoid pigment deposition in the red-fleshed and white-fleshed chinook salmon (Oncorhynchus tshawytscha) of Quesnel River, British Columbia

1986 ◽  
Vol 28 (4) ◽  
pp. 587-594 ◽  
Author(s):  
R. E. Withler
Keyword(s):  
British Columbia ◽  
Muscle Tissue ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Genetic Model ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Flesh Colour ◽  
Tissue Samples ◽  
Threshold Trait

Inheritance of the ability to deposit coloured dietary carotenoid pigments in muscle tissue was examined in 16 seapen-reared families of chinook salmon (Oncorhynchus tshawytscha) from the Quesnel River, British Columbia. The progeny red:white ratio varied significantly among families in two sample periods but not between sample periods for individual families. There was no difference between the sexes in proportions of red and white individuals. Total carotenoid extraction of muscle tissue samples from 152 progeny revealed that white individuals contained less carotenoid per gram of tissue (0.24 ± 0.04 μg) than did red ones (3.37 ± 0.14 μg). Estimates of the heritability of flesh colour, when treated as a threshold trait, were 0.93 (sire component) and 0.71 (dam component). A genetic model that invokes two genetic loci, each with two alleles, was proposed to explain the inheritance of flesh colour in Quesnel River chinook salmon. At each locus, one copy of a "red-determining" allele is required for coloured carotenoid pigments to be deposited in muscle tissue. The anomalous red:white ratios among the progeny of one male parent could not be accounted for by tetrasomy or pseudolinkage in conjunction with the proposed model.Key words: Oncorhynchus, salmon, carotenoids, heritability.

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