Effect of sevoflurane and halothane anesthesia on cognitive function and immune function in young rats

Objective: This study aims to show that impairment of cognitive function occurred during aging is related to increased oxidative stress. Methods: A total of 36 Sprague Dawley rats were divided into four groups: Young (3 months), middle (14 months), and old age groups (18 and 23 months). Rats were killed and blood was collected for the measurement of oxidative stress which includes deoxyribonucleic acid (DNA) damage and lipid peroxidation (malondialdehyde [MDA] levels). Cognitive function of rats was measured through open-field experiments, Morris water maze (MWM), and object identification. Results: Increased DNA damage and MDA levels were found in middle age and old rats compared to young rats (3 months old, p<0.05). There was an increase in anxiety with age as indicated by the increased production of fecal boli and decreased activity of grooming and rearing. For the navigation test, older rats took a long time to search for the hidden platform compared to young rats. In the probe test (spatial memory test 24 h after the last training), the middle- and old-age groups spent less time at the quadrant compared to the young age group. Conclusion: There is a decline in cognitive function with increased oxidative stress in aging rats.

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Impact of Visceral Obesity on Chronic Obstipation, Inflammation, Immune Function and Cognitive Function Among Patients with Inflammatory Bowel Disease

10.21203/rs.3.rs-37390/v1 ◽

2020 ◽

Author(s):

Yemin Wan ◽

Dan Zhang ◽

Yunzhi Qian ◽

Shuchen Chang ◽

Haihua Qian

Keyword(s):

Inflammatory Bowel Disease ◽

Adipose Tissue ◽

Cognitive Function ◽

Immune Function ◽

Bowel Disease ◽

Visceral Obesity ◽

Cross Sectional Study ◽

Cross Sectional ◽

Inflammatory Bowel ◽

The Impact

Abstract Background: Obesity has gained attention among patients with inflammatory bowel disease (IBD). The impact of visceral obesity on chronic obstipation, inflammation, immune function and cognition after diagnosis of IBD is still unknown.Methods: This is a cross-sectional study of 140 IBD patients. Patients’ visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were measured by abdominal computerized tomography (CT) scans and were grouped according to visceral obesity. Baseline variables, chronic obstipation status, inflammation status and immune function were compared. The implications of visceral obesity on cognitive function were evaluated using Mini-Mental State Examination (MMSE).Results: The prevalence of visceral obesity was 51% (37 out of 72) for CD patients and 26% for UC patients (18 out of 68 patients). CD patients with visceral obesity has higher incidence of chronic obstipation (81% vs. 57%, P = 0.028), higher IL-6 levels (9.3 vs. 6.0 pg/ml, P = 0.045) and lower CD4+ T cells (32.7% vs. 44.0%, P = 0.034). For UC patients, patients with visceral obesity have the tendency of higher IL-6 levels (7.2 vs. 6.0 pg/ml, P = 0.053).Conclusion: IBD patients had high risks of visceral obesity. Patients with visceral obesity had higher prevalence of chronic obstipation, higher inflammation levels, decreased immune function.

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Visceral obesity on chronic constipation, inflammation, immune function and cognitive function in patients with inflammatory bowel disease

10.21203/rs.3.rs-52908/v1 ◽

2020 ◽

Author(s):

Yemin Wan ◽

Dan Zhang ◽

Yunzhi Qian ◽

Shuchen Chang ◽

Haihua Qian

Keyword(s):

Inflammatory Bowel Disease ◽

Adipose Tissue ◽

Cognitive Function ◽

Immune Function ◽

Bowel Disease ◽

Chronic Constipation ◽

Visceral Obesity ◽

Cross Sectional ◽

Inflammatory Bowel ◽

The Impact

Abstract Objective: Obesity has gained attention among patients with inflammatory bowel disease (IBD). The impact of visceral obesity on chronic constipation, inflammation, immune function and cognition after diagnosis of IBD is still unknown.Methods: This is a cross-sectional study of 140 IBD patients. Patients’ visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were measured by abdominal computerized tomography (CT) scans and were grouped according to visceral obesity. Baseline variables, chronic constipation status, inflammation status and immune function were compared. The implications of visceral obesity on cognitive function were evaluated using Mini-Mental State Examination (MMSE).Results: The prevalence of visceral obesity was 51% (37 out of 72) for CD patients and 26% for UC patients (18 out of 68 patients). CD patients with visceral obesity has higher incidence of chronic constipation (81% vs. 57%, P = 0.028), higher IL-6 levels (15.28 pg/ml vs. 9.429 pg/ml, P = 0.0073) and lower CD4+ T cells (32.7% vs. 44.0%, P < 0.001). For UC patients, patients with visceral obesity have the tendency of higher IL-6 levels (7.2 vs. 6.0 pg/ml, P = 0.053). VAT/SAT ratio is associated with BMI (r = 0.652, P < 0.001).Conclusions: IBD patients had high risks of visceral obesity. CD Patients with visceral obesity had higher prevalence of chronic constipation, higher inflammation levels, decreased immune function.

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EFFECTS OF CELL PHONE-EMITTED ELECTROMAGNETIC FIELD ON THE COGNITIVE FUNCTION IN YOUNG RATS: POSSIBLE ROLE OF ALPHA LIPOIC ACID SUPPLEMENTATION

Al-Azhar Medical Journal ◽

10.21608/amj.2019.50752 ◽

2019 ◽

Vol 48 (2) ◽

pp. 145-164

Author(s):

Samar M. M. Abd El Rahman ◽

Bataa M.A. El- Kafoury ◽

Enas A. Abdel-Hady ◽

Noha N. Lasheen ◽

Wesam El Bakly ◽

...

Keyword(s):

Electromagnetic Field ◽

Cognitive Function ◽

Lipoic Acid ◽

Cell Phone ◽

Alpha Lipoic Acid ◽

Acid Supplementation ◽

Young Rats

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The Protective Effects of Apigenin on Cognitive Function and The Brain Transcriptome in Old Mice

Innovation in Aging ◽

10.1093/geroni/igab046.2562 ◽

2021 ◽

Vol 5 (Supplement_1) ◽

pp. 687-687

Author(s):

Alyssa Cavalier ◽

Zachary Clayton ◽

David Hutton ◽

Cali McEntee ◽

Douglas Seals ◽

...

Keyword(s):

Cognitive Function ◽

Immune Function ◽

Test Performance ◽

Brain Aging ◽

Protective Effects ◽

Protein Signaling ◽

Brain Transcriptome ◽

Age Related ◽

Pathway Analyses ◽

The Brain

Abstract Age-related declines in cognitive function increase the risk of developing mild cognitive impairment and dementia, but select nutraceuticals (bioactive plant compounds) may hold promise for protecting the brain and improving cognitive function with age. Apigenin is a flavonoid nutraceutical found in chamomile and reported to inhibit multiple hallmarks of aging; however, it has not been studied in the context of brain aging specifically. We treated young (6 mo) and old (27 mo) C57BL/6N mice with apigenin (0.5 mg/mL in 0.2% carboxymethylcellulose) or control (0.2% carboxymethylcellulose) drinking water for 6 weeks. Then, we assessed cognitive function and performed RNA-seq to characterize global transcriptomic changes and potential mechanisms of action in the brain. We observed impaired novel object recognition (NOR) test performance (an index of learning/memory) in old vs. young control mice (P<0.0001), but old apigenin mice had ~3-fold higher NOR performance relative to old control mice (P=0.02). Transcriptomic analyses also showed age-associated gene expression changes related to immune function and inflammation, consistent with the established role of inflammation in brain aging. However, some of these key changes were reversed by apigenin. In fact, >300 genes were differentially expressed in old apigenin-treated mice vs. old controls, and the biological processes linked with these differences were related to innate and adaptive immune function, and cytokine and chemokine regulation. We are performing protein/signaling pathway analyses to elucidate downstream cellular changes associated with apigenin treatment, but our current results suggest apigenin may be a promising nutraceutical candidate for preventing brain aging.

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