A portable medical diagnostic device utilizing free-standing responsive polymer film-based biosensors and low-cost transducer for point-of-care applications

2020 ◽  
Vol 304 ◽  
pp. 127356
Author(s):  
Eleni I. Tzianni ◽  
Jan Hrbac ◽  
Dimitrios K. Christodoulou ◽  
Mamas I. Prodromidis
Keyword(s):  
Polymer Film ◽  
Point Of Care ◽  
Low Cost ◽  
Diagnostic Device ◽  
Free Standing ◽  
Responsive Polymer ◽  
Medical Diagnostic

scholarly journals Numerical flow simulation methods and additive manufacturing methods for the development of a flow optimised design of a novel point-of-care diagnostic device

2017 ◽  
Vol 3 (2) ◽  
pp. 619-622
Author(s):  
Manuel Dethloff ◽  
Hermann Seitz ◽  
Marc Dangers
Keyword(s):  
Additive Manufacturing ◽  
Point Of Care ◽  
Low Cost ◽  
Flow Simulation ◽  
Experimental Tests ◽  
Test System ◽  
Test Device ◽  
Diagnostic Device ◽  
Manufacturing Technologies ◽  
User Friendly

AbstractFor the development of a novel, user-friendly and low cost point-of-care diagnostic device for the detection of disease specific biomarker a flow optimised design of the test system has to be investigated. The resulting test system is characterised by a reduced execution period, a reduction of execution steps and an integrated waste management. Based on previous results, the current study focused on the design implementation of the fluidic requirements, e. g. tightness, inside the test device. With the help of fluid flow simulations (CFD – computational fluid dynamics) the flow behaviour inside the test device was analysed for different designs and arrangements. Prototypes generated from additive manufacturing technologies (PolyJet modeling) are used for validating the simulation results and further experimental tests.

scholarly journals Flow optimised design of a novel point-of-care diagnostic device for the detection of disease specific biomarkers

2016 ◽  
Vol 2 (1) ◽  
pp. 685-688
Author(s):  
Manuel Dethloff ◽  
Marc Dangers ◽  
Boris Wilmes ◽  
Hermann Seitz
Keyword(s):  
Fluid Dynamics ◽  
Computational Fluid Dynamics ◽  
Point Of Care ◽  
Low Cost ◽  
Test System ◽  
Experimental Investigations ◽  
Functional Design ◽  
Diagnostic Device ◽  
User Friendly ◽  
Disease Specific

AbstractFor the development of a novel, user-friendly and low cost point-of-care diagnostic device for the detection of disease specific biomarkers a flow optimised design of the test system is investigated. For this, computational fluid dynamics and rapid prototyping methods are used. The result is a functional design, which can be used for further experimental investigations.

scholarly journals P07.06 A low cost, hand-held point of care molecular diagnostic device for sexually transmitted infections

2015 ◽  
Vol 91 (Suppl 2) ◽  
pp. A122.2-A122
Author(s):  
RE Mackay ◽  
M Branavan ◽  
P Craw ◽  
A Naveenathayalan ◽  
ST Sadiq ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Point Of Care ◽  
Low Cost ◽  
Molecular Diagnostic ◽  
Diagnostic Device ◽  
Sexually Transmitted

scholarly journals A Low-Cost Biological Agglutination Assay for Medical Diagnostic Applications

2018 ◽  
Author(s):  
Nicolas Kylilis ◽  
Pinpunya Riangrungroj ◽  
Hung-En Lai ◽  
Valencio Salema ◽  
Luis Ángel Fernández ◽  
...  
Keyword(s):  
Diagnostic Tests ◽  
Point Of Care ◽  
Low Cost ◽  
Field Testing ◽  
Human Fibrinogen ◽  
Detection Range ◽  
Agglutination Assay ◽  
E Coli ◽  
Medical Diagnostic ◽  
Diagnostic Applications

ABSTRACTAffordable, easy-to-use diagnostic tests that can be readily deployed for point-of-care (POC) testing are key in addressing challenges in the diagnosis of medical conditions and for improving global health in general. Ideally, POC diagnostic tests should be highly selective for the biomarker, user-friendly, have a flexible design architecture and a low cost of production. Here we developed a novel agglutination assay based on wholeE. colicells surface-displaying nanobodies which bind selectively to a target protein analyte. As a proof-of-concept, we show the feasibility of this design as a new diagnostic platform by the detection of a model analyte at nanomolar concentrations. Moreover, we show that the design architecture is flexible by building assays optimized to detect a range of model analyte concentrations supported using straight-forward design rules and a mathematical model. Finally, we re-engineerE. colicells for the detection of a medically relevant biomarker by the display of two different antibodies against the human fibrinogen and demonstrate a detection limit as low as 10 pM in diluted human plasma. Overall, we demonstrate that our agglutination technology fulfills the requirement of POC testing by combining low-cost nanobody production, customizable detection range and low detection limits. This technology has the potential to produce affordable diagnostics for both field-testing in the developing world, emergency or disaster relief sites as well as routine medical testing and personalized medicine.

scholarly journals Colorimetric Diagnostic Capillary Enabled by Size Sieving in a Porous Hydrogel

Biosensors ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 130
Author(s):  
John Mello Camille C. Guzman ◽  
Sheng-Min Hsu ◽  
Han-Sheng Chuang
Keyword(s):  
Limit Of Detection ◽  
Capillary Tube ◽  
Point Of Care ◽  
Low Cost ◽  
Glass Capillary ◽  
Diagnostic Device ◽  
Early Screening ◽  
Resource Limited ◽  
Detection Of Biomarkers ◽  
Porous Hydrogel

Handy and disposable point-of-care diagnostics facilitate the early screening of severe diseases in resource-limited areas. To address urgent needs in inconvenient sites, a simple colorimetric diagnostic device equipped with a capillary tube with porous hydrogel and immunocomplex particles was developed for the rapid detection of biomarkers (16 min). In this device, probe particles attach to capture particles (dp = 40 µm) and form sandwiched immunocomplexes in the presence of target biomarkers, and a red color progressively emerges when the sandwiched immunocomplex particles are blocked by the porous hydrogel embedded inside the glass capillary. Colorimetric aggregation was recorded using a smartphone and analyzed with imaging software. The limit of detection reached 1 ng/mL and showed a maximum of 79% accuracy compared with that obtained through a conventional spectrophotometric technique. The level of a diabetic retinopathy (DR) biomarker, lipocalin-1 (LCN-1), was measured in 1 µL of a human tear sample and used in testing the practicability of the proposed device. All healthy subjects showed lower intensity levels than the other diabetic counterparts (proliferative DR or nonproliferative DR patients), implying the potential of this device in clinical applications. Overall, the diagnostic device facilitates point-of-care-testing and provides a low-cost (~1 USD), compact, and reliable tool for early diagnosis in resource-limited areas.

scholarly journals Validation of a point-of-care rapid diagnostic test for hepatitis C for use in resource-limited settings

2019 ◽  
Vol 11 (4) ◽  
pp. 314-315
Author(s):  
James S Leathers ◽  
Maria Belen Pisano ◽  
Viviana Re ◽  
Gertine van Oord ◽  
Amir Sultan ◽  
...  
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Rapid Diagnosis ◽  
Direct Acting Antivirals ◽  
Resource Limited Settings ◽  
Rapid Diagnosis Test ◽  
Resource Limited ◽  
Specificity And Sensitivity ◽  
Hcv Screening ◽  
Direct Acting

Abstract Background Treatment of HCV with direct-acting antivirals has enabled the discussion of HCV eradication worldwide. Envisioning this aim requires implementation of mass screening in resource-limited areas, usually constrained by testing costs. Methods We validated a low-cost, rapid diagnosis test (RDT) for HCV in three different continents in 141 individuals. Results The HCV RDT showed 100% specificity and sensitivity across different samples regardless of genotype or viral load (in samples with such information, 90%). Conclusions The HCV test validated in this study can allow for HCV screening in areas of need when properly used.

scholarly journals Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 3985
Author(s):  
Nan Wan ◽  
Yu Jiang ◽  
Jiamei Huang ◽  
Rania Oueslati ◽  
Shigetoshi Eda ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Clinical Diagnostics ◽  
Detection Methods ◽  
Capacitive Sensing ◽  
Application Potential ◽  
Mirna Detection ◽  
Mirna 25 ◽  
Low Sensitivity

A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.g., low sensitivity, long assay time, expensive equipment, trained personnel, or unsuitability for point-of-care. New methodologies are needed to overcome these limitations to allow rapid, sensitive, low-cost, easy-to-use, and portable methods for miRNA detection at the point of care. In this work, to overcome these shortcomings, we integrated capacitive sensing and alternating current electrokinetic effects to detect specific miRNA-16b molecules, as a model, with the limit of detection reaching 1.0 femto molar (fM) levels. The specificity of the sensor was verified by testing miRNA-25, which has the same length as miRNA-16b. The sensor we developed demonstrated significant improvements in sensitivity, response time and cost over other miRNA detection methods, and has application potential at point-of-care.

scholarly journals A glucose meter interface for point-of-care gene circuit-based diagnostics

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Evan Amalfitano ◽  
Margot Karlikow ◽  
Masoud Norouzi ◽  
Katariina Jaenes ◽  
Seray Cicek ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Synthetic Biology ◽  
Molecular Diagnostics ◽  
Point Of Care ◽  
Low Cost ◽  
Gene Circuit ◽  
Reporter Systems ◽  
Glucose Meter ◽  
Pandemic Response ◽  
Glucose Output

AbstractRecent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential to provide decentralized and low-cost molecular diagnostics. However, it remains a challenge to deliver this sensing capacity into the hands of users in a practical manner. Here, we leverage the glucose meter, one of the most widely available point-of-care sensing devices, to serve as a universal reader for these decentralized diagnostics. We describe a molecular translator that can convert the activation of conventional gene circuit-based sensors into a glucose output that can be read by off-the-shelf glucose meters. We show the development of new glucogenic reporter systems, multiplexed reporter outputs and detection of nucleic acid targets down to the low attomolar range. Using this glucose-meter interface, we demonstrate the detection of a small-molecule analyte; sample-to-result diagnostics for typhoid, paratyphoid A/B; and show the potential for pandemic response with nucleic acid sensors for SARS-CoV-2.

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