Electrohydrodynamic jet 3D printing of PCL/PVP composite scaffold for cell culture

Talanta ◽  
2020 ◽  
Vol 211 ◽  
pp. 120750 ◽  
Author(s):  
Kai Li ◽  
Dazhi Wang ◽  
Kuipeng Zhao ◽  
Kedong Song ◽  
Junsheng Liang
Author(s):  
N Pradeep ◽  
C Chandrasekhara Sastry ◽  
Lc Brandão ◽  
B.S. Meennakshi

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1962 ◽  
Author(s):  
Abdullah M. Cakmak ◽  
Semra Unal ◽  
Ali Sahin ◽  
Faik N. Oktar ◽  
Mustafa Sengor ◽  
...  

Three-dimensional (3D) printing application is a promising method for bone tissue engineering. For enhanced bone tissue regeneration, it is essential to have printable composite materials with appealing properties such as construct porous, mechanical strength, thermal properties, controlled degradation rates, and the presence of bioactive materials. In this study, polycaprolactone (PCL), gelatin (GEL), bacterial cellulose (BC), and different hydroxyapatite (HA) concentrations were used to fabricate a novel PCL/GEL/BC/HA composite scaffold using 3D printing method for bone tissue engineering applications. Pore structure, mechanical, thermal, and chemical analyses were evaluated. 3D scaffolds with an ideal pore size (~300 µm) for use in bone tissue engineering were generated. The addition of both bacterial cellulose (BC) and hydroxyapatite (HA) into PCL/GEL scaffold increased cell proliferation and attachment. PCL/GEL/BC/HA composite scaffolds provide a potential for bone tissue engineering applications.


2018 ◽  
Vol 10 (27) ◽  
pp. 3364-3374 ◽  
Author(s):  
Akash S. Munshi ◽  
Chengpeng Chen ◽  
Alexandra D. Townsend ◽  
R. Scott Martin

Here we show that separate modules fabricated using 3D printing technology can be easily assembled to quantitate the amount of nitric oxide released from endothelial cells following ATP stimulation.


2019 ◽  
Vol 6 (1) ◽  
pp. 20 ◽  
Author(s):  
Haobo Yuan

Research related with scaffold engineering tends to be cross-domain and miscellaneous. Several realms may need to be focused simultaneously, including biomedicine for cell culture and 3D scaffold, physics for dynamics, manufacturing for technologies like 3D printing, chemistry for material composition, as well as architecture for scaffold’s geometric control. As a result, researchers with different backgrounds sometimes could have different understanding towards the product described as ‘Scaffold’. After reviewing the literature, numerous studies termed their developed scaffold as ‘novel’, compared with scaffolds previously designed by others using comparing criterion like ‘research time’, ‘manufacturing method’, ‘geometry’, and so on. While it may have been convenient a decade ago to, for example, categorize scaffold with ‘Dualistic Thinking’ logic into ‘simple-complicated’ or ‘traditional-novel’, this method for categorizing ‘novelty’ and distinguishing scaffold is insufficiently persuasive and precise when it comes to modern or future scaffold. From this departure of philosophical language, namely the language of ‘relativity’, it is important to distinguish between different scaffolds. Other than attempting to avoid ambiguity in perceiving scaffold, this language also provides clarity regarding the ‘evolution stage’ where the focused scaffolds currently stand, where they have been developed, and where in future they could possibly evolve.


Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 152
Author(s):  
Cacie Hart ◽  
Charles M. Didier ◽  
Frank Sommerhage ◽  
Swaminathan Rajaraman

The widespread adaptation of 3D printing in the microfluidic, bioelectronic, and Bio-MEMS communities has been stifled by the lack of investigation into the biocompatibility of commercially available printer resins. By introducing an in-depth post-printing treatment of these resins, their biocompatibility can be dramatically improved up to that of a standard cell culture vessel (99.99%). Additionally, encapsulating resins that are less biocompatible with materials that are common constituents in biosensors further enhances the biocompatibility of the material. This investigation provides a clear pathway toward developing fully functional and biocompatible 3D printed biosensor devices, especially for interfacing with electrogenic cells, utilizing benchtop-based microfabrication, and post-processing techniques.


RSC Advances ◽  
2015 ◽  
Vol 5 (37) ◽  
pp. 29335-29345 ◽  
Author(s):  
KyoungHo Lee ◽  
Cho-Rong Seo ◽  
Jin-Mo Ku ◽  
Hyeongjin Lee ◽  
Hyeon Yoon ◽  
...  

A new composite scaffold consisting of poly(ε-caprolactone), alginate, and phenamil was manufactured by a combined process, 3D-printing and coating process, for hard tissue regeneration.


2018 ◽  
Vol 5 (4) ◽  
pp. 041109 ◽  
Author(s):  
Max J. Lerman ◽  
Josephine Lembong ◽  
Greg Gillen ◽  
John P. Fisher

2021 ◽  
Author(s):  
Qiyuan Dai ◽  
Qingtao Li ◽  
Huichang Gao ◽  
Longtao Yao ◽  
Zefeng Lin ◽  
...  

The increasing insight of molecular and cellular processes within angiogenic cascade enhances the survival and integration of engineered bone constructs. Cu-doped bioactive glass (Cu-BG) now is a potential structural component...


Author(s):  
Koby Kubrin ◽  
Adeel Ahmed ◽  
Shkenca Demiri ◽  
Maria Majid ◽  
Ian M. Dickerson ◽  
...  

Template based chemical vapor deposition (CVD) is a process of effectively fabricating nanostructures such as Carbon nanotube arrays (CNT). During this process, a carbon-carrying precursor gas is used to deposit a layer of solid carbon on the surface of a template within a furnace. Template-based CVD using porous anodized aluminum oxide (AAO) membranes as the template has been applied to efficiently mass-produce CNT arrays which have shown promise for use in gene transfection applications. These AAO membranes are incredibly fragile, making them prone to cracks during handling which can compromise their performance. In order to ease handling of the CNT devices, three-dimensional (3D) printing has been applied to create a support structure for the fragile membranes. The work presented here focuses on the use of 3D printing as a means of integrating CNT arrays into nanofluidic devices, both increasing their useful application and preventing damage to the fragile arrays during handling. 3D printing allows the CNT arrays to be completely encapsulated within the fluidic device by printing a base of material before inserting the arrays. Additionally, 3D printing has been shown to create an adequate seal between the CNT arrays and the printed device without the need for additional adhesives or sealing processes. For this work, a commercially available, fused deposition modeling (FDM) 3D printer was used to print the devices out of polylactic acid (PLA) plastic. This approach has been shown to be effective and repeatable for nanofluidic device construction, while also being cost effective and less time consuming than other methods such as photolithography. Cell culture and has been demonstrated using HEK293 cells on the devices and was found to be comparable to tissue culture polystyrene.


Sign in / Sign up

Export Citation Format

Share Document