Effects of flaxseed dietary supplementation on sperm quality and on lipid composition of sperm subfractions and prostatic granules in rabbit

2010 ◽  
Vol 73 (5) ◽  
pp. 629-637 ◽  
Author(s):  
E. Mourvaki ◽  
R. Cardinali ◽  
A. Dal Bosco ◽  
L. Corazzi ◽  
C. Castellini
Keyword(s):  
Lipid Composition ◽  
Sperm Quality ◽  
Dietary Supplementation

scholarly journals Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

2017 ◽  
Vol 38 (5) ◽  
pp. 3069 ◽  
Author(s):  
Ana Carolina Rodrigues ◽  
Camila Montanari Ruiz ◽  
Carla Daniela Dan De Nardo ◽  
Gabriele Barros Mothé ◽  
Fabiano Martinez Rossi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Dietary Supplementation ◽  
Reproductive Efficiency ◽  
Omega 3 ◽  
Oral Supplementation ◽  
Animal Reproduction ◽  
Fresh Semen

For years, fatty acids have been recommended as a dietary supplement to improve canine hair. For animal reproduction, supplementation with omegas has been used to increase the reproductive efficiency and conception rate, but few studies have been conducted in dogs. The aim of this study was to evaluate the effects of daily dietary supplementation with omega-3 and -6 on the quality of fresh and frozen/thawed semen in canines. Semen was collected from seven dogs and evaluated for sperm motility, vigor, concentration, and morphology. The 17-week study included 119 ejaculates and was divided according to oral supplementation with omega-3 and -6: M1 (1st-5th week) or pre-supplementation; M2 (6th-9th week) and M3 (10th-13th week) or during supplementation; and M4 (14th-17th week) or post-supplementation. After analysis, the semen was frozen and then revaluated both immediately and 30 minutes (at 37° C) after thawing. Supplementation with omegas increased sperm motility, vigor, and concentration; however, supplementation had no influence on semen freezability. In addition, there was no improvement in sperm motility after supplementation when the thawed cells were maintained at 37° C for 30 minutes. We concluded that dietary supplementation with omega-3 and -6 for 4 to 8 weeks can improve the quality of fresh semen, although it has no effect on the freezability of canine semen.

scholarly journals Impact of cryopreservation on lipid composition of reproductive cells of male sterlets (Acipenser Ruthenus L.)

2018 ◽  
Vol 5 (1) ◽  
pp. 73-83
Author(s):  
L. Drahan ◽  
S. Veselsky ◽  
Yu. Rud ◽  
L. Buchatsky
Keyword(s):  
Lipid Composition ◽  
Low Temperatures ◽  
Neutral Lipids ◽  
Sperm Quality ◽  
Phospholipid Bilayer ◽  
Standard Equipment ◽  
Slowing Down ◽  
Acipenser Ruthenus ◽  
Adaptive Property ◽  
The Impact

Aim. To estimate the impact of low temperatures on the lipid composition of reproductive cells of male sterlets (Acipenser ruthenus L) in cryopreservation environment. Methods. The determination of sperm quality (color, consistence, concentration and motility of spermatozoa) was estimated by common biochemical methods using standard equipment. Thin-layer chromatography was used to reveal fi ve fractions of neutral lipids in the sperm of investigated fi sh, which were distributed on the plates as follows: the starting line demonstrated phospholipids, cholesterol, free fatty acids, triacylglycerol and ethers of cholesterol. The adaptive property of sperm was estimated by the level of spermatozoa survival prior to and after cryopreservation. Results. The obtained results of sterlet sperm activity prior to and after cryopreservation demonstrate high quality of native sperm and its considerable deterioration after freezing/thawing. Conclusions. The impact of low temperatures on the lipid composition of reproductive cells of male sterlets (Acipenser ruthenus L) in cryopreservation environment leads to impairments of the phospholipid bilayer of their membranes. It was established that during the thawing of frozen sperm cells which preserved their viability after cryopreservation their motility and capability of fertilizing an ovum were somewhat restored with slowing down of sperm cell motility.

scholarly journals FTIR Spectroscopy to Reveal Lipid and Protein Changes Induced on Sperm by Capacitation: Bases for an Improvement of Sample Selection in ART

2020 ◽  
Vol 21 (22) ◽  
pp. 8659
Author(s):  
Maria Pachetti ◽  
Luisa Zupin ◽  
Irene Venturin ◽  
Elisa Mitri ◽  
Rita Boscolo ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sample Selection ◽  
Poor Quality ◽  
Interesting Possibility ◽  
The Many ◽  
Α Helix ◽  
Analytical Approaches

Although being a crucial step for Assisted Reproduction Technologies (ART) success, to date sperm selection is based only on morphology, motility and concentration characteristics. Considering the many possible alterations, there is a great need for analytical approaches allowing more effective sperm selections. The use of Fourier Transform Infrared (FTIR) may represent an interesting possibility, being able to reveal many macromolecular changes in a single measurement in a nondestructive way. As a proof of concept, in this observational study, we used a FTIR approach to reveal features related to sperm quality and chemical changes promoted by in vitro capacitation. We found indication that α-helix content is increased in capacitated sperm, while high percentages of the β-structures seem to correlate to poor-quality spermatozoa. The most interesting observation was related to the lipid composition, when measured as CH2/CH3 vibrations (ratio 2853/2870), which resulted in being strongly influenced by capacitation and well correlated with sperm motility. Interestingly, this ratio is higher than 1 in infertile samples, suggesting that motility is related to sperm membranes stiffness and lipid composition. Although further analyses are requested, our results support the concept that FTIR can be proposed as a new smart diagnostic tool for semen quality assessment in ART.

Effect of dietary supplementation with amino acids on boar sperm quality and fertility

2016 ◽  
Vol 172 ◽  
pp. 182-189 ◽  
Author(s):  
Hong-Jun Dong ◽  
De Wu ◽  
Sheng-Yu Xu ◽  
Qiang Li ◽  
Zheng-Feng Fang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Sperm Quality ◽  
Dietary Supplementation ◽  
Boar Sperm

scholarly journals Season-induced variation in lipid composition is associated with semen quality in Holstein bulls

Reproduction ◽  
2013 ◽  
Vol 145 (5) ◽  
pp. 479-489 ◽  
Author(s):  
N Argov-Argaman ◽  
K Mahgrefthe ◽  
Y Zeron ◽  
Z Roth
Keyword(s):  
Fatty Acid ◽  
Lipid Composition ◽  
Semen Quality ◽  
Sperm Quality ◽  
Structural Parameters ◽  
Lipid Extraction ◽  
Sperm Head ◽  
High Saturated Fatty Acid ◽  
Seminal Fluids ◽  
Induced Variation

Season-induced variation in fatty acid and cholesterol composition in bovine semen has been associated with semen quality. Given the specific roles of the various semen compartments (seminal fluids, sperm head, and sperm tail) in fertilization, we hypothesized that environmental-stress-induced alterations in the lipid composition of a specific compartment might impair semen quality and sperm function. Semen samples were collected from five mature Holstein–Friesian bulls during the summer (August to September) and winter (December to January). Semen was evaluated by computerized sperm-quality analyzer, calibrated for bulls' semen, and centrifuged to separate the spermatozoa from the seminal fluids. The spermatozoal fraction was sonicated to separate the sperm head and tail compartments. Cold lipid extraction was performed with chloroform:methanol (2:1, vol/vol). Lipids were identified and quantified by gas chromatography. Seasonal variation was found in both physiological and structural parameters. The proportion of spermatozoa defined as morphologically normal was higher in the winter, with higher motility, progressive motility, and velocity relative to summer samples. Lipid composition within fractions varied between seasons with prominent impairment in the tail compartment, characterized by high saturated fatty acid, low polyunsaturated fatty acid, and low cholesterol concentrations during the summer. Given the association between alterations in lipid composition and reduced sperm motility and velocity during the summer, it is suggested that lipid composition might serve to predict sperm quality.

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