First report on the detection of the plasmid-borne colistin resistance gene mcr-1 in multi-drug resistant E. coli isolated from domestic and sewer waters in Syrian refugee camps in Lebanon

2019 ◽  
Vol 30 ◽  
pp. 117-120 ◽  
Author(s):  
Abd Allah Alhaj Sulaiman ◽  
Issmat I. Kassem
Keyword(s):  
Resistance Gene ◽  
Refugee Camps ◽  
Drug Resistant ◽  
Colistin Resistance ◽  
First Report ◽  
E Coli ◽  
Syrian Refugee

scholarly journals ISKpn40-Mediated Mobilization of the Colistin Resistance Gene mcr-3.11 in Escherichia coli

2020 ◽  
Vol 64 (10) ◽  
Author(s):  
Yu-Zhang He ◽  
Teng-Fei Long ◽  
Cai-Ping Chen ◽  
Bing He ◽  
Xing-Ping Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Wild Type ◽  
Colistin Resistance ◽  
Content Type ◽  
First Report ◽  
E Coli ◽  
Target Sites

ABSTRACT The mobile colistin resistance gene mcr-3 has globally disseminated since it was first reported in 2017 in Escherichia coli. In vitro mobilization assays in this study demonstrate the functionality of the composite transposon structure ISKpn40-mcr-3.11-dgkA-ISKpn40 in wild-type and recA− E. coli strains. These transpositions generated 4-bp duplications at the target sites. This is the first report demonstrating the mobility of the mcr-3.11 gene by transposition.

First report of Escherichia coli carrying the mobile colistin resistance gene mcr-1 in Turkey

2018 ◽  
Vol 15 ◽  
pp. 169-170 ◽  
Author(s):  
Cemil Kurekci ◽  
Muhsin Aydin ◽  
Ozkan Ufuk Nalbantoglu ◽  
Aycan Gundogdu
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Colistin Resistance ◽  
First Report

scholarly journals Plasmid mediated colistin resistant mcr-1 and co-existence of OXA-48 among Escherichia coli from clinical and poultry isolates: first report from Nepal

Gut Pathogens ◽  
2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Bijaya Muktan ◽  
Upendra Thapa Shrestha ◽  
Binod Dhungel ◽  
Bagish Chandra Mishra ◽  
Nabaraj Shrestha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Colistin Resistance ◽  
Clinical Specimens ◽  
E Coli ◽  
Resistant Genes ◽  
Rectal Swabs

Abstract Background Plasmid-mediated resistance to the last-resort drugs: carbapenems and colistin is an emerging public health threat. The studies on the prevalence and co-expression of resistant genes among livestock and human pathogens are rare in Nepal. This is the first study in Nepal exploring the prevalence and co-existence of colistin resistance gene, mcr-1 along with carbapenemase resistance gene, OXA-48 in Escherichia coli isolated from poultry and clinical specimens. Methods A total of 240 rectal swabs from chickens of five different poultry farms of Kathmandu valley and 705 mid-stream urine samples from human subjects attending Kantipur Hospital, Kathmandu were collected between August, 2018 and March, 2019. Rectal swabs and urine specimens were cultured. E. coli isolated from the specimens were screened for antimicrobial susceptibility testing (AST) using disk diffusion method’. Minimum inhibitory concentration (MIC) of colistin was determined by agar dilution method using 0.5 µg/ml to 32 µg/ml. The E. coli isolates were first screened for mcr-1 followed by screening for OXA-48 genes using conventional Polymerase chain reaction (PCR). Results Of the total samples analyzed, E. coli was isolated from 31.7% (76/240) of poultry and 7.9% (56/705) of clinical specimens. In AST, 80% (61/76) of E. coli from poultry and 79% (44/56) from clinical specimens were MDR. The phenotypic prevalence of colistin resistance in poultry specimens were 31.6% (24/76) and clinical specimens were 21.4% (12/56). In PCR assay, 27.6% (21/76) of poultry and 19.6% (11/56) of clinical isolates had colistin resistant mcr-1 gene. MICs value of E. coli isolates ranged from 4 to 32 (µg/ml) in both clinical and poultry isolates. Prevalence of co-existing carbapenem resistance gene, OXA-48, among colistin resistant mcr-1 positive isolates was 38% (8/21) in poultry specimens and 18.2% (2/11) in clinical specimens. Conclusions The high prevalence of colistin and carbapenem resistant genes, and their co-existence in plasmid DNA of E. coli isolates in this study suggests the possible spread to other animal, human and environmental pathogens. Molecular methods in addition to the conventional diagnostics in laboratories can help in early diagnosis, effective management and control of their potential transmission.

First report of the plasmid-mediated colistin resistance gene mcr-1 in a clinical Escherichia coli isolate in Algeria

2016 ◽  
Vol 48 (6) ◽  
pp. 760-761 ◽  
Author(s):  
Betitera Yanat ◽  
Jesús Machuca ◽  
Radia Dali Yahia ◽  
Abdelaziz Touati ◽  
Álvaro Pascual ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Colistin Resistance ◽  
Coli Isolate ◽  
First Report

scholarly journals Emergence of Colistin Resistance Gene mcr-1 in Cronobacter sakazakii Producing NDM-9 and in Escherichia coli from the Same Animal

2016 ◽  
Vol 61 (2) ◽  
Author(s):  
Bao-Tao Liu ◽  
Feng-Jing Song ◽  
Ming Zou ◽  
Zhi-Hui Hao ◽  
Hu Shan
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Cronobacter Sakazakii ◽  
Colistin Resistance ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant

ABSTRACT We report the presence of mcr-1 in Escherichia coli and carbapenem-resistant Cronobacter sakazakii from the same diseased chicken. The mcr-1 gene linked with ISApl1 was located on two different IncI2 plasmids, including one multidrug plasmid in E. coli, whereas fosA3-bla NDM-9 was on an IncB/O plasmid in C. sakazakii. The development of the fosA3-bla NDM-9 resistance region was mediated by IS26. The colocation of mcr-1 or bla NDM-9 with other resistance genes will accelerate the dissemination of the two genes.

scholarly journals Mechanistic Insights into Crosstalk of Tet(X) and MCR-1, Two Resistance Enzymes Co-produced by A Single Plasmid

2020 ◽  
Author(s):  
Yongchang Xu ◽  
Lizhang Liu ◽  
Huimin Zhang ◽  
Youjun Feng
Keyword(s):  
Resistance Gene ◽  
Infection Model ◽  
Global Public Health ◽  
Genetic Determinants ◽  
Colistin Resistance ◽  
E Coli ◽  
New Variant ◽  
Single Plasmid

AbstractTigecycline and colistin are few of last-resort defenses used in anti-infection therapies against carbapenem-resistant bacterial pathogens. The successive emergence of plasmid-borne tet(X) tigecycline resistance mechanism and mobile colistin resistance (mcr) determinant, renders them clinically ineffective, posing a risky challenge to global public health. Here, we report that co-carriage of tet(X6) and mcr-1 gives co-resistance to both classes of antibiotics by a single plasmid in E. coli. Genomic analysis suggested that transposal transfer of mcr-1 proceeds into the plasmid pMS8345A, in which a new variant tet(X6) is neighbored with Class I integron. The structure-guided mutagenesis finely revealed the genetic determinants of Tet(X6) in the context of phenotypic tigecycline resistance. The combined evidence in vitro and in vivo demonstrated its enzymatic action of Tet(X6) in the destruction of tigecycline. The presence of Tet(X6) (and/or MCR-1) robustly prevents the accumulation of reactive oxygen species (ROS) induced by tigecycline (and/or colistin). Unlike that mcr-1 exerts fitness cost in E. coli, tet(X6) does not. In the tet(X6)-positive strain that co-harbors mcr-1, tigecycline resistance is independently of colistin resistance caused by MCR-1-mediated lipid A remodeling, and vice versa. Co-production of Tet(X6) and MCR-1 gives no synergistic delayed growth of the recipient E. coli. Similar to that MCR-1 behaves in the infection model of G. mellonella, Tet(X6) renders the treatment of tigecycline ineffective. Therefore, co-transfer of such two AMR genes is of great concern in the context of “one health” comprising environmental/animal/human sectors, and heightened efforts are required to monitor its dissemination.Author summaryWe report that tet(X6), a new tigecycline resistance gene, is co-carried with the other resistance gene mcr-1 by a single plasmid. Not only have we finely mapped genetic determinants of tet(X6), but also revealed its biochemical action of tigecycline destruction. Crosstalk of Tet(X6) with MCR-1 is addressed. Tet(X6) tigecycline resistance is independently of MCR-1 colistin resistance, and vice versa. Similar to MCR-1 that renders colistin clinically ineffective, Tet(X6) leads to the failure of tigecycline treatment in the infection model of G. mellonella. This study extends mechanistic understanding mechanism and interplay of Tet(X6) and MCR-1, coproduced by a single plasmid. It also heightens the need to prevent rapid and large-scaled spread of AMR.

scholarly journals First report of the mcr-1 colistin resistance gene identified in two Escherichia coli isolates from clinical samples, Philippines, 2018

2020 ◽  
Vol 21 ◽  
pp. 291-293
Author(s):  
John Mark S. Velasco ◽  
Ma. Theresa G. Valderama ◽  
Katie R. Margulieux ◽  
Paula Corazon S. Diones ◽  
Angeli Mae B. Reyes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Clinical Samples ◽  
Colistin Resistance ◽  
First Report

scholarly journals First Report of an Escherichia coli Strain Carrying the Colistin Resistance Determinant mcr-1 from a Dog in South Korea

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 768
Author(s):  
Dong Chan Moon ◽  
Abraham Fikru Mechesso ◽  
Hee Young Kang ◽  
Su-Jeong Kim ◽  
Ji-Hyun Choi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South Korea ◽  
Companion Animals ◽  
Coli Strain ◽  
Colistin Resistance ◽  
First Report ◽  
E Coli ◽  
Risk Gene ◽  
Additional Resistance ◽  
Third Generation Cephalosporins

We studied the presence of the mobile colistin resistance gene mcr-1 in Escherichia coli isolates recovered from fecal and urine samples of companion animals, that were collected from South Korea in 2018 and 2019. The mcr-1 gene was detected in one colistin-resistant E. coli isolated from a diarrheic dog. The isolate exhibited additional resistance to multiple antimicrobials, including fluoroquinolones and third-generation cephalosporins. The mcr-1 carrying isolate belonged to ST160. The pulsed-field gel electrophoresis pattern of our strain differed from those ST160 E. coli strains previously identified from chickens in Korea. The mcr-1 gene was identified in the IncI2 plasmid. It was also transferred to E. coli J53 recipient strain, with a conjugation efficiency of 2.8 × 10−4. Average nucleotide identity analysis demonstrated that the mcr-1-carrying plasmid in this study was closely related to those from patients in Korea. To the best of our knowledge, this is the first report of mcr-1 carrying E. coli from a companion animal in South Korea. Our findings support One Health approach is necessary to prevent the dissemination of this high-risk gene.

scholarly journals Complete Genome Sequence of a Colistin Resistance Gene ( mcr-1 )-Bearing Isolate of Escherichia coli from the United States

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Richard J. Meinersmann ◽  
Scott R. Ladely ◽  
James L. Bono ◽  
Jodie R. Plumblee ◽  
M. Carolina Hall ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Resistance Gene ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
The United States ◽  
Colistin Resistance ◽  
E Coli

Transmissible colistin resistance conferred by the mcr-1 gene-bearing IncI2 plasmid has been recently reported in Escherichia coli in the United States. We report here the completed genome sequence of a second E. coli strain isolated from swine in the United States that carried the mcr-1 gene on an IncI2-type plasmid.

Sign in / Sign up

Export Citation Format

Share Document