scholarly journals Plasmid mediated colistin resistant mcr-1 and co-existence of OXA-48 among Escherichia coli from clinical and poultry isolates: first report from Nepal

Gut Pathogens ◽  
2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Bijaya Muktan ◽  
Upendra Thapa Shrestha ◽  
Binod Dhungel ◽  
Bagish Chandra Mishra ◽  
Nabaraj Shrestha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Colistin Resistance ◽  
Clinical Specimens ◽  
E Coli ◽  
Resistant Genes ◽  
Rectal Swabs

Abstract Background Plasmid-mediated resistance to the last-resort drugs: carbapenems and colistin is an emerging public health threat. The studies on the prevalence and co-expression of resistant genes among livestock and human pathogens are rare in Nepal. This is the first study in Nepal exploring the prevalence and co-existence of colistin resistance gene, mcr-1 along with carbapenemase resistance gene, OXA-48 in Escherichia coli isolated from poultry and clinical specimens. Methods A total of 240 rectal swabs from chickens of five different poultry farms of Kathmandu valley and 705 mid-stream urine samples from human subjects attending Kantipur Hospital, Kathmandu were collected between August, 2018 and March, 2019. Rectal swabs and urine specimens were cultured. E. coli isolated from the specimens were screened for antimicrobial susceptibility testing (AST) using disk diffusion method’. Minimum inhibitory concentration (MIC) of colistin was determined by agar dilution method using 0.5 µg/ml to 32 µg/ml. The E. coli isolates were first screened for mcr-1 followed by screening for OXA-48 genes using conventional Polymerase chain reaction (PCR). Results Of the total samples analyzed, E. coli was isolated from 31.7% (76/240) of poultry and 7.9% (56/705) of clinical specimens. In AST, 80% (61/76) of E. coli from poultry and 79% (44/56) from clinical specimens were MDR. The phenotypic prevalence of colistin resistance in poultry specimens were 31.6% (24/76) and clinical specimens were 21.4% (12/56). In PCR assay, 27.6% (21/76) of poultry and 19.6% (11/56) of clinical isolates had colistin resistant mcr-1 gene. MICs value of E. coli isolates ranged from 4 to 32 (µg/ml) in both clinical and poultry isolates. Prevalence of co-existing carbapenem resistance gene, OXA-48, among colistin resistant mcr-1 positive isolates was 38% (8/21) in poultry specimens and 18.2% (2/11) in clinical specimens. Conclusions The high prevalence of colistin and carbapenem resistant genes, and their co-existence in plasmid DNA of E. coli isolates in this study suggests the possible spread to other animal, human and environmental pathogens. Molecular methods in addition to the conventional diagnostics in laboratories can help in early diagnosis, effective management and control of their potential transmission.

scholarly journals Detection of Plasmid-Mediated Colistin Resistant mcr-1 Gene in Escherichia coli Isolated from Infected Chicken Livers in Nepal

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2060
Author(s):  
Sayara Bista ◽  
Upendra Thapa Shrestha ◽  
Binod Dhungel ◽  
Pragya Koirala ◽  
Tulsi Ram Gompo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Conventional Polymerase Chain Reaction ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Human Pathogens ◽  
Colistin Resistance ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Agar Dilution

Background: Plasmid-mediated resistance to the colistin in poultry is considered as an emerging problem worldwide. While poultry constitutes the major industry in Nepal, there is a paucity of evidence on colistin resistance in Escherichia coli isolates causing natural infections in poultry. This study aimed to explore the prevalence of plasmid-mediated colistin resistance gene, mcr-1 in E. coli isolated from liver samples of dead poultry suspected of E. coli infections. Methods: A total of two hundred and seventy liver samples (227 broilers and 43 layers) from dead poultry suspected of colibacillosis were collected from post-mortem in the Central Veterinary Laboratory (CVL), Kathmandu, between 1 February and 31 July 2019. The specimens were processed to isolate and identify E. coli; an antimicrobial susceptibility test (AST) using disk diffusion method was performed with 12 different antibiotics: Amikacin (30 µg), ampicillin (10 µg), ciprofloxacin (5 µg), chloramphenicol (30 µg), cefoxitin (30 µg), ceftazidime (30 µg), ceftriaxone (30 µg), cotrimoxazole (25 µg), gentamicin (10 µg), imipenem (10 µg), levofloxacin (5 µg) and tetracycline (30 µg). Colistin resistance was determined by agar dilution method and colistin-resistant strains were further screened for plasmid-mediated mcr-1 gene, using conventional polymerase chain reaction (PCR). Results: Out of 270 liver samples, 53.3% (144/270) showed growth of E. coli. The highest number (54%; 109/202) of E. coli isolates was obtained in the liver samples from poultry birds (of both types) aged less than forty days. In AST, 95.1% (137/144) and 82.6% (119/144) of E. coli isolates were resistant against tetracycline and ciprofloxacin, respectively, while 13.2% (19/144) and 25.7% (37/144) isolates were resistant to cefoxitin and imipenem, respectively. In the same assay, 76.4% (110/144) E. coli isolates were multi-drug resistant (MDR). The phenotypic prevalence of colistin resistance was 28.5% (41/144). In the PCR assay, 43.9% (18/41) of colistin-resistant isolates were screened positive for plasmid-mediated mcr-1. Conclusion: The high prevalence of mcr-1 in colistin-resistant E. coli isolates in our study is a cause of concern for the probable coming emergence of colistin resistance in human pathogens, due to horizontal transfer of resistant genes from poultry to human isolates.

scholarly journals Susceptibility pattern of Escherichia coli from urinary tract infections to antibiotics and methanol extracts of Olax subscorpioidea and Sida corymbosa

2020 ◽  
Vol 17 (1) ◽  
pp. 75-80
Author(s):  
Philip A. Idowu ◽  
Babatunde M. Okanlawon ◽  
Habeeb O. Salam
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Traditional Use ◽  
E Coli ◽  
Methanol Extracts ◽  
Tract Infections

Antibiotic resistance in urinary tract infections (UTIs), of which Escherichia coli causes about 80% of cases is on increase, causing mortality, morbidity and increased health care costs. Clinical isolates of E. coli (13) from UTIs were tested for susceptibility to standard antibiotics and extracts of Olax subscorpioidea and Sida corymbosa. Methanol extracts of the plants were screened at 20, 40, 80 and 100 mg/ml against the isolates using agar-welldiffusion method while antibiogram was determined by Kirby-Bauer disc diffusion method. The minimum inhibitory concentrations (MICs) of the plants` extracts and two antibiotics were determined by agar dilution method. The isolates were mostly susceptible to ofloxacin and 100% resistance to augmentin. Extracts of the plants showed good and dose-dependent activities, even on the multidrug resistant E. coli isolates. The zones of inhibition of the extracts ranged 9-16 mm while the MICs ranged 0.5-10 mg/ml on the isolates. This study has shown that MDR E. coli in UTIs are still prevalent and that the roots of O. subscorpioidea and stem of S. corymbosa extracts have good antibacterial activities against the isolates. The results justified the traditional use of the plants to treat infections generally and the potential utilization in the treatment of UTIs. Keywords: Urinary tract infection; Antibiotics; Escherichia coli; Olax subscorpioidea; Sida corymbosa

scholarly journals Emergence of Colistin Resistance Gene mcr-1 in Cronobacter sakazakii Producing NDM-9 and in Escherichia coli from the Same Animal

2016 ◽  
Vol 61 (2) ◽  
Author(s):  
Bao-Tao Liu ◽  
Feng-Jing Song ◽  
Ming Zou ◽  
Zhi-Hui Hao ◽  
Hu Shan
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Cronobacter Sakazakii ◽  
Colistin Resistance ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant

ABSTRACT We report the presence of mcr-1 in Escherichia coli and carbapenem-resistant Cronobacter sakazakii from the same diseased chicken. The mcr-1 gene linked with ISApl1 was located on two different IncI2 plasmids, including one multidrug plasmid in E. coli, whereas fosA3-bla NDM-9 was on an IncB/O plasmid in C. sakazakii. The development of the fosA3-bla NDM-9 resistance region was mediated by IS26. The colocation of mcr-1 or bla NDM-9 with other resistance genes will accelerate the dissemination of the two genes.

scholarly journals Complete Genome Sequence of a Colistin Resistance Gene ( mcr-1 )-Bearing Isolate of Escherichia coli from the United States

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Richard J. Meinersmann ◽  
Scott R. Ladely ◽  
James L. Bono ◽  
Jodie R. Plumblee ◽  
M. Carolina Hall ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Resistance Gene ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
The United States ◽  
Colistin Resistance ◽  
E Coli

Transmissible colistin resistance conferred by the mcr-1 gene-bearing IncI2 plasmid has been recently reported in Escherichia coli in the United States. We report here the completed genome sequence of a second E. coli strain isolated from swine in the United States that carried the mcr-1 gene on an IncI2-type plasmid.

scholarly journals Genome Sequence of Avian Escherichia coli Strain IHIT25637, an Extraintestinal Pathogenic E. coli Strain of ST131 Encoding Colistin Resistance Determinant MCR-1

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Christa Ewers ◽  
Stephan Göttig ◽  
Maria Bülte ◽  
Sophie Fiedler ◽  
Manuela Tietgen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Resistance Gene ◽  
Genome Sequence ◽  
Coli Strain ◽  
Sequence Type ◽  
Resistance Determinant ◽  
Colistin Resistance ◽  
E Coli ◽  
Expec Strain

Sequence type 131 (ST131) is one of the predominant Escherichia coli lineages among extraintestinal pathogenic E. coli (ExPEC) that causes a variety of diseases in humans and animals and frequently shows multidrug resistance. Here, we report the first genome sequence of an ST131-ExPEC strain from poultry carrying the plasmid-encoded colistin resistance gene mcr-1 .

scholarly journals Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016

2016 ◽  
Vol 21 (27) ◽  
Author(s):  
Basil Britto Xavier ◽  
Christine Lammens ◽  
Rohit Ruhal ◽  
Samir Kumar-Singh ◽  
Patrick Butaye ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Epidemiological Surveillance ◽  
Nucleotide Identity ◽  
Colistin Resistance ◽  
Gram Negative ◽  
E Coli ◽  
Data Call

We identified a novel plasmid-mediated colistin-resistance gene in porcine and bovine colistin-resistant Escherichia coli that did not contain mcr-1. The gene, termed mcr-2, a 1,617 bp phosphoethanolamine transferase harboured on an IncX4 plasmid, has 76.7% nucleotide identity to mcr-1. Prevalence of mcr-2 in porcine colistin-resistant E. coli (11/53) in Belgium was higher than that of mcr-1 (7/53). These data call for an immediate introduction of mcr-2 screening in ongoing molecular epidemiological surveillance of colistin-resistant Gram-negative pathogens.

scholarly journals Study of antimicrobial effect of some plants of Lamiaceae family on Escherichia coli species isolated from children with urinary tract infection

2018 ◽  
Vol 8 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Hossein Emad Momtaz ◽  
Shirin Moradkhan ◽  
Mohammad Yousef Alikhani ◽  
Farzaneh Esnaashari ◽  
Mojdeh Afkhami
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Side Effects ◽  
Tract Infection ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Dilution Method ◽  
Herbal Extracts ◽  
E Coli

Introduction: Urinary tract infection (UTI) has a high prevalence in children. It is an important cause of renal scar, chronic renal disease, failure to thrive and hypertension. The unnecessary consumption of antibiotics results in the bacterial resistance to most common drugs. In addition, possible drug allergic reaction and side effects should be considered. Therefore, finding new antimicrobial compounds is necessary. Objectives: In this study, the antimicrobial effect of some plants of Lamiaceae family on Escherichia coli in comparison with common antibiotics used in UTI was investigated. Materials and Methods: In this experimental study, the hydroalcoholic extract of 13 plant species from Lamiaceae family were prepared and their antimicrobial effects were assessed by the agar well diffusion method. In addition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were investigated using the serial dilution method. The zone of inhibition (ZOI) of the growth of E. coli was compared between the studied extracts and five most common antibiotics in the treatment of UTI. Results: The mean diameter of ZOI was significantly higher for amikacin than the studied extracts and other antibiotics. Among the herbal extracts, the highest ZOI was observed for Nepeta crispa. In addition, the MIC and MBC of N. crispa were significantly lower than other extracts. Conclusion: According to the side effects and relatively high cost of chemical and synthetic drugs, use of herbal extracts, such as N. crispa may be recommended as an alternative treatment of UTI with E. coli. The results of present study are promising to introduce new candidates in the treatment of UTI.

scholarly journals An Epidemiological Study of Drug Resistance and Resistance Genes in Bovine Escherichia coli Isolates in Heilongjiang Province of China

2018 ◽  
Vol 46 (1) ◽  
pp. 9
Author(s):  
Jia-San Zheng ◽  
Ting-Ting Zhu ◽  
Yun Liu ◽  
Ting Liu ◽  
Yan-Qing Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Heilongjiang Province ◽  
Antibacterial Drugs ◽  
Resistance Phenotype ◽  
E Coli

Background: To explore the epidemiology of bovine multidrug-resistant Escherichia coli isolates and resistance genes in Heilongjiang province of China. This study examined the prevalence of genes in bovine E. coli isolates, which confer resistance to antibiotics that are commonly used in the clinic, in regions of Baiquan, Shangzhi, and Songbei of Harbin. The purpose of the study was to investigate the epidemiology of the main resistance genes of bovine E. coli isolates in clinical veterinary medicine, and to provide a theoretical basis for preventing the spread of drug-resistant bacteria, as well as for rational drug use.Materials, Methods & Results: The sensitivity of 105 isolates to 22 antibiotics was determined using the KirbyBauer disk diffusion method, and the distribution of 19 kinds of common drug resistance genes was investigated using Polymerase Chain Reaction. The results showed that the resistance rate to nine antibiotics was over 50%, including rifampin (84.76%), ampicillin (73.58%), tetracycline (69.52%), and sulfisoxazole (59.05%). In total, 105 strains of bovine E. coli presented 21 spectra of drug resistance, including eight strains (7.62%, 8/105) that were resistant to one antibiotic and four strains (3.81%, 4/105) that were resistant to 21 antibiotics. The resistance gene detection results showed that the streptomycin-resistance gene strA was found in 73 isolates, accounting for 69.52% of the isolates, followed by the sulfanilamide-resistance genes sul3/sul2 and the aminoglycoside-resistance gene aphA, which accounted for 57.14%, 51.43%, and 50.48%, respectively, of the isolates.Discussion: This study revealed serious drug resistance of bovine E. coli isolates in some areas of Heilongjiang province. Of 105 E. coli isolates, more than 50% were resistant to the following antibacterial drugs: rifampicin, ampicillin, tetracycline, sulfisoxazole, and cephalothin. The isolates were the most sensitive to amikacin, with a sensitivity of 84.76%, followed by sensitivity to ofloxacin, ciprofloxacin, norfloxacin, cefoxitin, and tobramycin. Drug sensitivity tests showed that the drug resistance spectra of the bovine E. coli isolates was different in different regions, indicating that there were multidrug-resistant bovine E. coli isolates in different regions of Heilongjiang province, and that drug resistance differed among different regions. This may be due to prolonged use or overuse of antibiotics in a particular locality. Additionally, because of different management modes of livestock farms, the application of antimicrobial drugs in some farms may have imposed selective pressure on the intestinal flora including E. coli, resulting in the horizontal transmission of drug resistance among the bacteria. The study found that some strains had a resistance phenotype, but no resistance gene, while some had a resistance gene without expressing a resistance phenotype, which is consistent with relevant reports in the literature. This may be related to the same genotype corresponding to different resistance phenotypes, or different levels of gene expression, or different drug metabolic rates. In our study, some strains with certain drug resistance genes were sensitive to the corresponding drug, which may be due to mutations of drug-resistance genes, the loss of a strains resistance phenotype, or the loss of gene function. These issues require further study. This study revealed serious drug resistance of bovine E. coli isolates in some areas of Heilongjiang province. Of 105 E. coli isolates, more than 50% were resistant to the following antibacterial drugs: rifampicin, ampicillin, tetracycline, sulfisoxazole, and cephalothin. The isolates were the most sensitive to amikacin, with a sensitivity of 84.76%, followed by sensitivity to ofloxacin, ciprofloxacin, norfloxacin, cefoxitin, and tobramycin.

scholarly journals Novel plasmid-mediated colistin resistance mcr-4 gene in Salmonella and Escherichia coli, Italy 2013, Spain and Belgium, 2015 to 2016

2017 ◽  
Vol 22 (31) ◽  
Author(s):  
Alessandra Carattoli ◽  
Laura Villa ◽  
Claudia Feudi ◽  
Ludovica Curcio ◽  
Serenella Orsini ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Gene Variant ◽  
Colistin Resistance ◽  
E Coli ◽  
Novel Gene ◽  
Serovar Typhimurium

A novel mcr colistin resistance gene was identified in a strain of Salmonella enterica, monophasic variant of serovar Typhimurium (4,5,12:i:- ), isolated from a pig at slaughter in Italy in 2013, and in Escherichia coli strains collected during routine diagnostic of post-weaning diarrhoea in pigs from Spain and Belgium in 2015 and 2016. Immediate implementation of mcr-screening including this novel gene variant is required for Salmonella and E. coli from humans and food-producing animals in Europe.

scholarly journals ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACT FROM STEM BARK OF Cinnamomum sintoc

2017 ◽  
Vol 17 (2) ◽  
pp. 77
Author(s):  
Nurdin Saidi ◽  
Hira Helwati ◽  
Lailatul Qhadariah Lubis ◽  
Muhammad Bahi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Stem Bark ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
E Coli ◽  
Paper Disk ◽  
Agar Dilution

Antimicrobial activity of methanol extract from stem bark of Cinnamomum sintoc has been evaluated against Candida albicans, Staphylococcus aureus and Escherichia coli. The extraction of compound was carried out by maceration, then isolation by column chromatograph, which yielded five (5) subfractions (A-E). Activity against fungus C. albicans, S. aureus bacteria dan E. coli using agar dilution method in paper disk. Methanol extract was not potent against antifungal activity but shows antibacterial activity with medium category. Subfraction C showed that antibacterial activity against S. aureus and E. coli with weak category, but subfractions D and E did not show any activity.

Sign in / Sign up

Export Citation Format

Share Document