Free radical scavenging and anti-apoptotic effects of vitamin C towards N-nitrosopiperidine and N-nitrosodibutylamine-induced apoptosis

2008 ◽  
Vol 180 ◽  
pp. S181
Author(s):  
Almudena García ◽  
Paloma Morales ◽  
Nuria Arranz ◽  
Eugenia Delgado ◽  
Ana I. Haza
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Induced Apoptosis ◽  
Apoptotic Effects

scholarly journals Antioxidant and antimicrobial activities against Enterobacter cloacae of extracts of Eclipta alba Hassk.

2016 ◽  
Vol 19 (4) ◽  
pp. 76-83
Author(s):  
Trang Thi Xuan Dai ◽  
Anh Thi Tu Vo
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Enterobacter Cloacae ◽  
Free Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Eclipta Alba

This study was subjected to investigate antimicrobial and antioxidant properties of Eclipta alba extracts (methanol, hexane, chloroform and ethyl acetate extracts). The antioxidant property of the extract was assessed by 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Kirby-Bauer method was used to determine the antibacterial activity against Enterobacter cloacae of the extracts. The extracts of Eclipta alba were tested against E. cloacae. The highest antibacterial potentiality was exhibited by the ethyl acetate extract of E. alba leaves, means of zones of bacterial growth inhibition are 26.3 mm at a concentration of 32 μg/mL. DPPH free radical scavenging effect of the extracts was compared with standard antioxidant vitamin C. The highest antioxidant activity was exhibited by the ethyl acetate extract of E. alba leaves. The result also showed that the DPPH scavenging activity of ethyl acetate extract from leaves of E. alba was high (EC50 = 419.38 μg/mL). However, this result was 18.99 times lower than that of vitamin C (EC50 = 22.08 μg/mL).

scholarly journals Partition Behavior in Aqueous Two-Phase System and Antioxidant Activity of Flavonoids from Ginkgo biloba

2018 ◽  
Vol 8 (11) ◽  
pp. 2058 ◽  
Author(s):  
Can Liu ◽  
Shuochen Liu ◽  
Lingli Zhang ◽  
Xiaoxue Wang ◽  
Lanqing Ma
Keyword(s):  
Antioxidant Activity ◽  
Polyethylene Glycol ◽  
Free Radical ◽  
Vitamin C ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phase System ◽  
Two Phase ◽  
Extraction Parameters ◽  
Partition Behavior

In this work, the aqueous two-phase extraction parameters and in vitro antioxidant activity of total flavonoids from Ginkgo biloba were investigated. The partition behavior of the flavonoids in an aqueous two-phase system (ATPS) was analyzed, the extraction parameters were optimized using response surface methodology, and the antioxidant activity of the flavonoids was determined by free-radical scavenging tests (1,1-diphenyl-2-picrylhydrazyl (DPPH) assay) and the ferric reducing/antioxidant power (FRAP) assay. The results showed that the concentration of ammonium sulfate was negatively correlated with the phase-volume ratio, whereas the concentration of polyethylene glycol was positively correlated. The maximum yield of flavonoids (4.11 mg g−1) was obtained under the following optimal extraction conditions: Concentration of polyethylene glycol (PEG)1500 30% and (NH4)2SO4 22% (mass fraction), and liquid/solid ratio 40:1 (mL g−1). The antioxidant activity tests showed that the flavonoids from the G. biloba leaf exhibited free-radical scavenging activity, with an IC50 of 2.66 mg L−1, which was superior to that of vitamin C. The free-radical scavenging ability of the flavonoids was proportional to the flavonoid concentration. The total reducing power of the Ginkgo flavonoids was slightly lower than that of vitamin C. In this study, the distribution of flavonoids in an ATPS was analyzed and a mathematical model for the ATPS extraction of Ginkgo flavonoids was established, which provides a reference for further development and utilization of G. biloba.

scholarly journals Aktivitas Penangkalan Radikal Bebas dan Kemampuan Reduksi Ekstrak Kulit Kayu Akway (Drimys piperita Hook. f.)

2019 ◽  
Vol 7 (4) ◽  
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Mathelda Kurniaty Roreng ◽  
Elva Intan Permatasari ◽  
Dolly Citra Manalu ◽  
...  
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenol ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Akway (Drimys piperita Hook. f) merupakan tumbuhan yang termasuk dalam kelompok tumbuhan berkayu, berdaun tebal aromatik dan termasuk kerabat winteraceae. Tumbuhan ini dimanfaatkan sebagai obat tradisional untuk mengobati malaria dan untuk meningkatkan vitalitas tubuh. Beberapa penelitian kandungan fitokimia ekstrak akway telah dilakukan untuk mengetahui potensi bioaktivitas akway. Ekstrak kulit kayu akway dilaporkan mengandung kelompok senyawa alkaloid, saponin, tanin, flavonoid, terpenoid dan glikosida. Minyak atsiri kulit kayu akway mengandung linalool, β-pinen, α-pinen, nerolidol dan terpineol. Senyawa-senyawa tersebut dilaporkan memiliki aktivitas antioksidan yang tinggi. Tujuan penelitian ini adalah untuk menentukan kapasitas antioksidan ekstrak kulit kayu akway secara in vitro dan vitamin C sebagai kontrol positif. Pengujian kapasitas antioksidan yang dilakukan meliputi kandungan total fenol dengan metode Folin-Ciocalteu, kandungan flavonoid dengan metode aluminum klorida, kapasitas penangkalan radikal bebas menggunakan metode DPPH (1,1-Diphenyl-2-picrylhydrazyl)-radical scavenging assay dan daya reduksi menggunakan metode reduksi Fe+3 menjadi Fe+2. Hasil penelitian menunjukkan bahwa ekstrak metanol memiliki kandungan total fenol dan flavonoid yang tertinggi dibandingkan dengan ekstrak etanol dan etilasetat. Kandungan total fenol dan flavonoid ekstrak metanol masing-masing sebesar 18,22 dan 14,32%. Ekstrak metanol dan vitamin C memiliki kapasitas menangkal radikal bebas DPPH dan daya reduksi yang paling tinggi kemudian diikuti ekstrak etanol dan etilasetat. Kemampuan menangkal radikal bebas ekstrak metanol dan vitamin C pada konsentrasi 200 µg/ml masing-masing sebesar 90% dan 88,31% sedangkan daya reduksi masing-masing sebesar 0,54 dan 0,62. Kesimpulannya, ekstrak metanol memiliki kapasitas antioksidan yang paling tinggi dibandingkan dengan ekstrak etanol dan etilasetat. Abstract Free Radical Scavenging Activity and Reducing Power of Akway (Drimys piperita Hook. f.) Bark ExtractsAkway (Drimys piperita Hook. f) was a woody and aromatic plant of winteraceae. This plant was used as traditional medical plant to heal malaria and to enhance vitality of body. Some studies were done to know bioactivity potency of akway extracts. D. piperita bark extract contains alkaloid, saponin, tanin, flavonoid, terpenoid and glycoside. The bark essential oil of the plant consists of lynalool, β-pinene, α-pinene, nerolidol and terpineol. Those compounds were exhibited high antioxidant activity. The objectives of this research were to determine total phenol and flavonoid of the extracts and its antioxidant capacity which was compared to antioxidant capacity of vitamin C. The assay of antioxidant capacity comprised of total phenol and flavonoid content, free radical scavenging activity, and reducing power. Total phenol and flavonoid was determined using Folin-Ciocalteu and aluminum chloride method, respectively while determination of free radical scavenging activity and reducing power using DPPH-radical scavenging and Fe+3 to Fe+2 reducing power method, respectively. The results indicated that methanol extract had the highest in total phenol and flavonoid content. Total phenol and flavonoid of methanol extract were 18.22% and 14.32%, respectively. Vitamin C and methanol extract had the highest DPPH (1,1-Diphenyl-2-picrylhydrazyl)-radical scavenging activity and reducing power, respectively. DPPH-radical scavenging activity of methanol extract and vitamin C in concentration of 200 µg/ml were 90% and 88.31%, respectively while its reducing power were 0.54 and 0,62, respectively. As conclusion, methanol extract had the highest antioxidant activity compared with ethanol and ethyl acetate extracts.

scholarly journals EVALUATION OF FREE RADICAL SCREENING AND ANTIOXIDANT POTENTIAL OF MORINGA CONCANENSIS NIMMO-A MEDICINAL PLANT USED IN INDIAN TRADITIONAL MEDICATION SYSTEM

2018 ◽  
Vol 10 (7) ◽  
pp. 91
Author(s):  
Brindha Banu Balakrishnan ◽  
Kalaivani Krishnasamy
Keyword(s):  
Free Radical ◽  
Vitamin E ◽  
Vitamin C ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Antioxidant Potential ◽  
Glutathione S Transferase ◽  
Pre Treatment

Objective: We aimed to investigate the free radical scavenging, antioxidant and hepatoprotective potential of M. concanensis Nimmo leaves.Methods: Free radical scavenging activity was evaluated by employing various accepted in vitro­ systems, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), hydroxyl (OH) and nitric oxide (NO) radical. Antioxidant potential of M. concanensis Nimmo extract was assessed against H2O2 in goat liver by determination of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glucose-6-phosphatase dehydrogenase (G-6-PDH), total reduced glutathione (GSH), vitamin C, vitamin E activity, and lipid peroxidation (LPO).Results: Results showed that the amount of plant extract of M. concanensis Nimmo required to scavenge 50% of the DPPH radicals was 401.80 μg/ml, ABTS radical was 353.14 μg/ml, OH radical was 433.71 μg/ml and NO radical was 371.24 μg/ml. Also, the pre-treatment of ethanolic extract of M. concanensis Nimmo leaves in goat liver showed a significant protection against H2O2 induced oxidative stress by retaining of antioxidants of SOD (4.76±0.25), CAT (26.81±0.25), GPx (6.41±0.34), GST (2.31±0.10), G-6-PDH (0.84±0.02), GSH (37.30±1.12), vitamin C (3.01±0.07), vitamin E (14.43±0.45) within normal range.Conclusion: In conclusion, promising free radical scavenging, the antioxidant activity of M. concanensis Nimmo leaves can be able to treat various diseases caused by free radicals.

scholarly journals ANALISIS FRAKSI AKTIF EKSTRAK DAUN GEDI HIJAU (Abelmoschus manihot L.) DALAM MENANGKAL RADIKAL BEBAS DPPH

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 187
Author(s):  
Maria T Atuani ◽  
Sri Sudewi ◽  
Defny S Wewengkang
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Mobile Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Fraction I

ABSTRACT Gedi (Abelmoschus manihot L.) is a tropical plant of family malvaceae, traditionally known in North Sulawesi as a vegetable plant. Gren gedi leaves from Bitung are known to have activity as free radical scavenging at extract level but have not been tested at the fraction level. This study aims to determine the free radical scavenging activity of the green gedi leaf fractions in counteraction DPPH (1,1-diphenil-2-picrylhydarzyl) free radical. Extraction was done by maceration method using ethanol solvent. The preliminary test of free radical scavenging was carried out by thin layer chromatography (TLC) using the stationary phase of silica gel F254 with the mobile phase of n-hexane : chloroform (1:2) and sprayed with DPPH 0,08 mM. Then it was fractionated using vacuum liquid chromatography (VLC) with the mobile phase based on its polarity, such as combination of n-hexane and ethyl acetate. Each fraction obtained was tested for free radical scavenging activity showed whitish yellow patches on a purple background, which indicates that extracts have free radical scavenging activity. The spectrophotometer measurement results showed that each leaf of green gedi leaves had weak free radical scavenging activity, but still was a free radical scavenger, ie fraction I had an IC50 value of 169,69 μg/mL; fraction II was 826,52 μg/mL; fraction III was 311,13 μg/mL; and fraction IV was 246,80 μg/mL. Fraction V has an IC50 value of 2294,64 μg/mL, this is indicates that the fraction V has no activity as a free radical scavenging compound, because it has very large IC50 value. While vitamin C as a comparison has very strong free radical scavenging activity with IC50 of 3,22 μg/mL. Keywords: Fraction, Extract, Abelmoschus manihot L., DPPH Method. ABSTRAK Gedi (Abelmoschus manihot L.) merupakan tumbuhan tropis family malvaceae, secara tradisional telah lama dikenal di Sulawesi Utara sebagai tanaman sayuran. Daun gedi hijau yang berasal dari Bitung diketahui memiliki aktivitas sebagai penangkap radikal bebas pada tingkat ekstrak namun belum diuji pada tingkat fraksi. Penelitian ini bertujuan untuk mengetahui aktivitas penangkap radikal bebas dari fraksi-fraksi daun gedi hijau dalam menangkal radikal bebas DPPH (1,1-diphenil-2-picrylhydarzyl). Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol. Uji pendahuluan aktivitas penangkap radikal bebas dilakukan secara kromatografi lapis tipis (KLT) menggunakan fase diam silika gel F254 dengan fase gerak n-heksana : kloroform (1:2) dan disemprot DPPH 0,08mM. Kemudian difraksinasi menggunakan kromatografi cair vakum (KCV) dengan fase gerak berdasarkan kepolarannya, yakni kombinasi n-heksana dan etil asetat. Hasil uji pendahuluan aktivitas penangkap radikal bebas secara KLT menunjukkan adanya bercak kuning keputih-putihan dengan latar belakang ungu, yang menandakan ekstrak memiliki aktivitas penangkap radikal bebas. Hasil pengukuran secara spektrofotometer menunjukkan bahwa setiap fraksi daun gedi hijau memiliki aktivitas penangkap radikal bebas yang lemah, namun tetap bersifat sebagai penangkap radikal bebas, yakni fraksi I memiliki nilai IC50 sebesar 169,69 μg/mL; fraksi II 826,52 μg/mL; fraksi III 311,13 μg/mL; dan fraksi IV 246,80 μg/mL. Fraksi V memiliki nilai IC50 sebesar 2294,64 μg/mL, menandakan fraksi V tidak memiliki aktivitas sebagai senyawa penangkap radikal bebas, karena memiliki nilai IC50 yang sangat besar. Sedangkan vitamin C sebagai pembanding memiliki aktivitas penangkap radikal bebas sangat kuat dengan IC50 sebesar 3,22 μg/mL.Kata Kunci : Fraksi, Ekstrak, Abelmoschus manihot L., Metode DPPH.

Comparative Assessment of Free Radical Scavenging Ability of Green and Red Cabbage Based on Their Antioxidant Vitamins and Phytochemical Constituents

2020 ◽  
Vol 16 (8) ◽  
pp. 1231-1241
Author(s):  
Faiza Ashfaq ◽  
Masood S. Butt ◽  
Ahmad Bilal ◽  
Saima Tehseen ◽  
Hafiz A.R. Suleria
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Hplc Analysis ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antioxidant Vitamins ◽  
Total Polyphenols ◽  
Antioxidant Power ◽  
Red Cabbage ◽  
Radical Scavenging Ability

Background: Nowadays, bioactive moieties of plants are gaining attention amongst the masses to mitigate lifestyle related dysfunctions owing to their safe nature and functional properties. Objective: Considering phytochemistry and cost-effectiveness of cabbage, the current project was designed to probe the antioxidant capacity of locally grown green and red cabbage. Methods: The total polyphenols and free radical scavenging ability of red and green cabbage were determined using spectrophotometer while HPLC analysis was carried out to further fractionate phenolic acids and flavonoid constituents. Apart from this, antioxidant vitamins including vitamin C tocopherol and β-carotene were also detected using HPLC system. Results: The red cabbage showed higher amount of total polyphenols and flavonoids (224.37±6.96 & 219.15±10.30 mg/100g F.W.) than green cabbage (58.41±3.01 & 34.04±1.06 mg/100g F.W.) along with the existence of anthocyanins (69.86±4.12 mg/100g F.W.) in red cabbage. Comparative HPLC analysis regarding antioxidant moieties showed significant proportion of kempferol (171.10±5.99 mg/100g F.W.) followed by vitamin C (139.07±2.23 mg/100g F.W.) in red cabbage however, vitamin C (121.46±3.28 mg/100g F.W.) was found as the major antioxidant in green cabbage. The red cabbage depicted higher free radical quenching and reducing ability in contrast to green cabbage using DPPH (1, 1-diphenyl-2-picrylhydrazyl), ABTS [2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)], FRAP (ferric reducing antioxidant power) and PFRAP (potassium ferricyanide reducing antioxidant power) and H2O2 scavenging ability assays. Conclusion: In the nutshell, red cabbage showed better free radical scavenging ability as compared to green cabbage based on variation and quantification of antioxidant indices.

Comparative in vitro Free Radical Scavenging Property of -carotene and Naringenin with Respect to Vitamin C and N-acetyl Cysteine

Pharmacologia ◽  
2012 ◽  
Vol 3 (12) ◽  
pp. 724-728 ◽  
Author(s):  
Pallab Kanti Haldar ◽  
Purbajit Chetia ◽  
Asis Bala ◽  
Bidita Khandelwal
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
C And N ◽  
Acetyl Cysteine

scholarly journals Uji Aktivitas Antioksidan Menggunakan Metode DPPH (2,2-difenil-1-pikrilhidrazil) Terhadap Ekstrak Etanol Kulit Buah Durian (Durio zibethinnus L.) dari Desa Alasmalang Kabupaten Banyumas

2021 ◽  
Vol 8 (1) ◽  
pp. 75
Author(s):  
Eko Prasetyo ◽  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Titi Pudji Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Secondary Metabolite ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Activity Assay ◽  
Dpph Method

Kulit durian merupakan bagian tanaman yang pernah diteliti sebelumnya dan mempunyai aktivitas farmakologi sebagai antioksidan. Kulit durian mengandung metabolit sekunder yaitu flavonoid, tanin dan alkaloid. Kulit durian diekstrak menggunakan pelarut etanol 70% dengan metode sokletasi.  Pengujian aktivitas antioksidan menggunakan metode pengukuran penangkapan radikal bebas oleh 1,1-difenil-2-pikrilhidrazil (DPPH). Vitamin C digunakan sebagai kontrol positif dengan nilai IC50 5,63 ppm dan ekstrak etanol sebesar 204,33 ppm. Penelitian ini menunjukkan bahwa ekstrak etanol kulit durian tidak memiliki aktivitas antioksidan menggunakan metode DPPH. Kata Kunci: Durian, Sokletasi, DPPH (2,2-difenil-1-pikrilhidrazil), antioksidan, IC50  Durian fruit peels (Durion zibethinus Murr.) have  been studied previously and reported to have phramacological activity that has the potential to be antioxidant. The durian fruits peels, contained secondary metabolite, namely flavonoids, tannin and alkaloid. Durian fruit peels (Durion zibethinus Murr.) were extracted with ethanol 70% with soxletation method. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay. Vitamin C was used as standard with IC50 5,63 ppm and the ethanol eztracts showed IC50 204,33 ppm. This study provided that Durian fruit peels (Durion zibethinus Murr.) cannot inhibit free radical usimg the DPPH method. 

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