Microcystin-leucine arginine (MC-LR) induces mouse ovarian inflammation by promoting granulosa cells to produce inflammatory cytokine via activation of cGAS-STING signaling

2022 ◽  
Author(s):  
Kunyang Liu ◽  
Xiaonan Zhao ◽  
Meihong Guo ◽  
Jinling Zhu ◽  
Dongmei Li ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Inflammatory Cytokine

Inhibition of Pro-Inflammatory Cytokine Secretion by Select Antioxidants in Human Coronary Artery Endothelial Cells

2020 ◽  
Vol 90 (1-2) ◽  
pp. 103-112 ◽  
Author(s):  
Michael J. Haas ◽  
Marilu Jurado-Flores ◽  
Ramadan Hammoud ◽  
Victoria Feng ◽  
Krista Gonzales ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ascorbic Acid ◽  
Coronary Artery ◽  
Inflammatory Cytokines ◽  
Inflammatory Cytokine ◽  
Culture Media ◽  
Cytokine Secretion ◽  
Human Coronary Artery ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

Abstract. Inflammatory and oxidative stress in endothelial cells are implicated in the pathogenesis of premature atherosclerosis in diabetes. To determine whether high-dextrose concentrations induce the expression of pro-inflammatory cytokines, human coronary artery endothelial cells (HCAEC) were exposed to either 5.5 or 27.5 mM dextrose for 24-hours and interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor α (TNF α) levels were measured by enzyme immunoassays. To determine the effect of antioxidants on inflammatory cytokine secretion, cells were also treated with α-tocopherol, ascorbic acid, and the glutathione peroxidase mimetic ebselen. Only the concentration of IL-1β in culture media from cells exposed to 27.5 mM dextrose increased relative to cells maintained in 5.5 mM dextrose. Treatment with α-tocopherol (10, 100, and 1,000 μM) and ascorbic acid (15, 150, and 1,500 μM) at the same time that the dextrose was added reduced IL-1β, IL-6, and IL-8 levels in culture media from cells maintained at 5.5 mM dextrose but had no effect on IL-1β, IL-6, and IL-8 levels in cells exposed to 27.5 mM dextrose. However, ebselen treatment reduced IL-1β, IL-6, and IL-8 levels in cells maintained in either 5.5 or 27.5 mM dextrose. IL-2 and TNF α concentrations in culture media were below the limit of detection under all experimental conditions studied suggesting that these cells may not synthesize detectable quantities of these cytokines. These results suggest that dextrose at certain concentrations may increase IL-1β levels and that antioxidants have differential effects on suppressing the secretion of pro-inflammatory cytokines in HCAEC.

Anti-inflammatory cytokine profile and mortality in febrile patients

The Lancet ◽  
1998 ◽  
Vol 351 (9107) ◽  
pp. 950-953 ◽  
Author(s):  
J VANDISSEL ◽  
P VANLANGEVELDE ◽  
R WESTENDORP ◽  
K KWAPPENBERG ◽  
M FROLICH
Keyword(s):  
Inflammatory Cytokine ◽  
Cytokine Profile ◽  
Anti Inflammatory ◽  
Anti Inflammatory Cytokine

Importance of the Renin-Angiotensin-System in human granulosa lutein cells – RAS in granulosa cells

2008 ◽  
Vol 68 (S 01) ◽  
Author(s):  
D Herr ◽  
WC Duncan ◽  
G Hack ◽  
R Konrad ◽  
R Kreienberg ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Renin Angiotensin System ◽  
Angiotensin System ◽  
Renin Angiotensin

Butyryl- and acetylcholine-esterases in human IVF-derived follicular fluid and luteinizing granulosa cells

2013 ◽  
Vol 121 (03) ◽  
Author(s):  
J Blohberger ◽  
D Einwang ◽  
D Berg ◽  
U Berg ◽  
S Hecht ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Follicular Fluid ◽  
Human Ivf

Corticotrophin-Releasing-Hormone (CRH) modulates ovarian steroid biosynthesis in human primary granulosa cells

2016 ◽  
Vol 76 (10) ◽  
Author(s):  
S Heublein ◽  
S Hecht ◽  
V Nick ◽  
S Mahner ◽  
C Thaler ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Steroid Biosynthesis ◽  
Ovarian Steroid ◽  
Releasing Hormone
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