Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples

2019 ◽  
Vol 267 ◽  
pp. 69-74 ◽  
Author(s):  
Ibrahim Abbas ◽  
Michael Hildreth
Keyword(s):  
Haemonchus Contortus ◽  
Peanut Agglutinin ◽  
Fecal Samples

scholarly journals Prevalence of Haemonchus contortus in Goats of Western Chitwan of Nepal

2017 ◽  
Vol 5 (3) ◽  
pp. 321-325 ◽  
Author(s):  
Kashyap Adhikari ◽  
Hari Bahadur Rana ◽  
Krishna Kaphle ◽  
Tanka Khanal ◽  
Rabin Raut
Keyword(s):  
Health Status ◽  
Haemonchus Contortus ◽  
Age Groups ◽  
Age Group ◽  
Cross Sectional ◽  
Fecal Samples ◽  
Total Prevalence ◽  
Medium Level ◽  
Cross Sectional Design ◽  
The Relationship

The research was done in cross sectional design to study about the prevalence of Haemonchus contortus in goats of Western Chitwan of Nepal. A total of 252 fecal samples were collected through purposive sampling. Fecal samples were examined through sedimentation and floatation method. Egg per gram (EPG) was calculated through Mac Master Counting Technique of positive samples. Total prevalence was found to be 13.89%. Infection of parasite was more prevalent in males (15.18%) than in female (12.86%) but was statistically non-significant (P>0.05). In non-dewormed goats (40.32%), the prevalence was significantly (P<0.05) higher than in dewormed goats (5.26%). The results regarding the relationship between different age groups of goats and H. contortus showed that highest prevalence (14.54%) of parasite was observed in age group of below 1 year and the lowest (12.96%) in age group of  above 2 year, statistically non-significant (P>0.05). Maximum goats were found with medium level of infestation through EPG. This study shows higher prevalence of Haemonchus in non-dewormed than in dewormed goats which is statistically significant at (P<0.05). Thus, effective deworming programme and management must be maintained in order to upgrade the health status and maximize the benefits from the animal.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 321-325

Modification and further evaluation of a fluorescein-labeled peanut agglutinin test for identification of Haemonchus contortus eggs

2010 ◽  
Vol 169 (1-2) ◽  
pp. 209-213 ◽  
Author(s):  
Megan E. Jurasek ◽  
Janell K. Bishop-Stewart ◽  
Bobby E. Storey ◽  
Ray M. Kaplan ◽  
Michael L. Kent
Keyword(s):  
Haemonchus Contortus ◽  
Peanut Agglutinin

High-throughput identification and quantification of Haemonchus contortus in fecal samples

2019 ◽  
Vol 265 ◽  
pp. 24-28
Author(s):  
Noélie Douanne ◽  
Victoria Wagner ◽  
Denise Bélanger ◽  
Christopher Fernandez-Prada
Keyword(s):  
High Throughput ◽  
Haemonchus Contortus ◽  
Fecal Samples ◽  
Identification And Quantification

scholarly journals The Incidence Rate of Helminthiasis in The Digestive Tract of Dairy Cattle in The Partnership of PT. Greenfields Indonesia

2020 ◽  
Vol 1 (1) ◽  
pp. 11
Author(s):  
Wenny Nur Azizah ◽  
Dony Chrismanto ◽  
Sri Pantja Madyawati ◽  
Hana Eliyani
Keyword(s):  
Dairy Cattle ◽  
Incidence Rate ◽  
Digestive Tract ◽  
Haemonchus Contortus ◽  
Helminth Species ◽  
Gastrointestinal Helminths ◽  
Fecal Samples ◽  
High Rainfall ◽  
Moniezia Expansa

Background: One of the partnerships of PT. Greenfields Indonesia is located on the slopes of Mount Kawi, precisely in Pijiombo, Ngadirenggo Village, Wlingi District, Blitar Regency. The tropical situation in this area is very humid and has high rainfall. It causes dairy cattle to be infected with helminths. Therefore, observations of the incidence rate of gastrointestinal helminthiasis in dairy cattle are needed. Purpose: To determine the prevalence of gastrointestinal helminths in dairy cattle in the partnership of PT. Greenfields Indonesia. Method: Fecal samples from 36 dairy cattle were collected and examined using native, sedimentation, and flotation techniques. Result: Indicated that the prevalence of gastrointestinal helminths in the dairy cattle was about 66,66%. There were 24 dairy cattle positively and 12 cattle negatively. Helminth species found included class Nematoda (Haemonchus contortus, Oesophagostomum radiatum, Bunostomum phlebotomum, Mecistocirrus digitatus, and Gaigeria pachyscelis) and Cestoda (Moniezia expansa). Conclusion: The incidence rate of helminthiasis was quite high dairy cattle in the partnership of PT. Greenfields Indonesia.

scholarly journals Nematode-Larvae Infestation in Goats Fed "Malojillo" (Pará Grass) and Merker Grasses in Puerto Rico

1969 ◽  
Vol 39 (1) ◽  
pp. 22-31
Author(s):  
H. Ciordia ◽  
J. D. Rivera-Anaya
Keyword(s):  
Puerto Rico ◽  
Fecal Sample ◽  
Haemonchus Contortus ◽  
The Other ◽  
Control Group ◽  
Blood Samples ◽  
Fecal Samples ◽  
Nematode Parasites ◽  
Nematode Larvae ◽  
Oesophagostomum Columbianum

1. The degree of nematode larvae infestation in goats fed malojillo (Pará grass) and Merker grass in Puerto Rico was investigated in an attempt to determine whether goats would become more significantly parasitized when eating either one of the two grasses. 2. The technique of raising and-maintaining parasite-free animals is described. 3. Blood samples for hemoglobin and packed-cell determinations and fecal samples for nematode ova counts were obtained every 15 days from the 10 experimental goats. 4. It was found that goats eating malojillo harbored a larger number of nematode parasites than those eating Merker grass. Ova of Strongyloides papillosus appeared in the feces of the goats even before the beginning of this work, but that infestation disappeared about a month before the end of the experiment. Haemonchus contortus ova were seen next, being followed chronologically by Trichostrongylus spp. and Oesophagostomum columbianum. The latter species was harbored only by the goats receiving malojillo. Goats eating Merker grass exhibited 8.9 ova per gram of fecal sample throughout the experiment, those receiving malojillo showed 48.2 eggs per sample, while those in the control group had only 0.57 eggs per sample. 5. All animals were sacrificed at the conclusion of this work and the digestive tracts thoroughly searched for helminths. More nematodes were secured from the goats eating malojillo than from the other two groups. A total of 1,028 nematodes was recovered from animals in the malojillo group, 191 from the Merker group, and only 3 from the control group receiving imported hay.

scholarly journals Prevalence of gastrointestinal worms in Wonosobo and thin-tailed sheep on the slope of Mount Sumbing, Central Java, Indonesia

2019 ◽  
Vol 12 (11) ◽  
pp. 1866-1871 ◽  
Author(s):  
Zein Ahmad Baihaqi ◽  
Irkham Widiyono ◽  
Wisnu Nurcahyo
Keyword(s):  
Sea Level ◽  
Haemonchus Contortus ◽  
Rainy Season ◽  
Dry Season ◽  
Fecal Samples ◽  
Counting Technique ◽  
Central Java ◽  
Helminth Eggs ◽  
Central Java Province ◽  
Dry And Rainy Seasons

Aim: This study was conducted to determine the prevalence of gastrointestinal (GI) worms in Wonosobo and thin-tailed sheep from the slope of Mount Sumbing. Materials and Methods: Fecal samples (n=305) were collected directly from the rectum of Wonosobo and thin-tailed sheep during the dry and rainy seasons in Wonosobo Regency, Central Java Province, Indonesia. The presence of GI helminth eggs in the fecal samples was assessed using the modified McMaster egg counting technique. The identification of the eggs or oocysts was done on the basis of their morphology and size. Results: The highest prevalence of GI worms was observed in male thin-tailed sheep (76.47%) during the rainy season, whereas the lowest prevalence was observed in female Wonosobo sheep (47.36%) during the dry season. The types of GI nematodes observed in these two types of sheep were Haemonchus contortus, Ostertagia spp., Trichostrongylus spp., Bunostomum spp., Trichuris spp., and Moniezia spp. The GI worms with the highest prevalence were of Haemonchus spp. and were observed in male thin-tailed sheep. The prevalences of the two types of sheep assessed at an altitude of 1150 m above sea level were higher than those observed at the altitude of 1586 m. The prevalence of clinical and sub-clinical parasites infestation in Wonosobo and thin-tailed sheep in Kwadungan village was significantly different (p<0.05). Conclusion: This study showed that two types of local sheep on the slope of Mount Sumbing are infected with various GI worms during the dry and rainy seasons. The highest prevalence of GI worms was found in thin-tailed sheep at an altitude of 1150 m above sea level during the rainy season, with H. contortus being the most prevalent GI parasites.

in vivo anthelmintic activity of Syzygium cumini leaves against Haemonchus contortus in sheep

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
LDR Oliveira ◽  
JPHV Miranda ◽  
GS Curado ◽  
JP Costa Neto ◽  
BF Santos ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Syzygium Cumini

Haemonchus contortus: in vivo anthelmintic activity of leaves of anacardium humile

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
LDR Oliveira ◽  
JPHV Miranda ◽  
GS Curado ◽  
JP Costa Neto ◽  
BF Santos ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity
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