scholarly journals Nematode-Larvae Infestation in Goats Fed "Malojillo" (Pará Grass) and Merker Grasses in Puerto Rico

1969 ◽  
Vol 39 (1) ◽  
pp. 22-31
Author(s):  
H. Ciordia ◽  
J. D. Rivera-Anaya
Keyword(s):  
Puerto Rico ◽  
Fecal Sample ◽  
Haemonchus Contortus ◽  
The Other ◽  
Control Group ◽  
Blood Samples ◽  
Fecal Samples ◽  
Nematode Parasites ◽  
Nematode Larvae ◽  
Oesophagostomum Columbianum

1. The degree of nematode larvae infestation in goats fed malojillo (Pará grass) and Merker grass in Puerto Rico was investigated in an attempt to determine whether goats would become more significantly parasitized when eating either one of the two grasses. 2. The technique of raising and-maintaining parasite-free animals is described. 3. Blood samples for hemoglobin and packed-cell determinations and fecal samples for nematode ova counts were obtained every 15 days from the 10 experimental goats. 4. It was found that goats eating malojillo harbored a larger number of nematode parasites than those eating Merker grass. Ova of Strongyloides papillosus appeared in the feces of the goats even before the beginning of this work, but that infestation disappeared about a month before the end of the experiment. Haemonchus contortus ova were seen next, being followed chronologically by Trichostrongylus spp. and Oesophagostomum columbianum. The latter species was harbored only by the goats receiving malojillo. Goats eating Merker grass exhibited 8.9 ova per gram of fecal sample throughout the experiment, those receiving malojillo showed 48.2 eggs per sample, while those in the control group had only 0.57 eggs per sample. 5. All animals were sacrificed at the conclusion of this work and the digestive tracts thoroughly searched for helminths. More nematodes were secured from the goats eating malojillo than from the other two groups. A total of 1,028 nematodes was recovered from animals in the malojillo group, 191 from the Merker group, and only 3 from the control group receiving imported hay.

scholarly journals Fasciola Hepatica and other Helminths in Goats in Puerto Rico

1969 ◽  
Vol 67 (4) ◽  
pp. 501-506
Author(s):  
P. Bendezú ◽  
A. D. Frame ◽  
R. Muñoz ◽  
J. Cabezas ◽  
J. Hurd ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Stool Sample ◽  
Haemonchus Contortus ◽  
Parasite Species ◽  
Fasciola Hepatica ◽  
The Other ◽  
Fecal Examination ◽  
Helminth Eggs ◽  
Oesophagostomum Columbianum ◽  
Concurrent Infections

Fecal examination of 769 goats taken in 1981 from 48 farms in 30 municipalities located throughout Puerto Rico, showed 10 helminths to be common in these goats. The study showed 1.43 percent of these to be positive with Fasciola hepatica eggs. The other helminth eggs found and percent rates of infection were as follows: Oesophagostomum columbianum 65.67, Haemonchus contortus 56.31, Bunostomum trigonocephalum 46.94, Chabertia ovina 24.57, Trichostrongylus capricola 15.60, Ostertagia circumcincta 14.43, Trichuris ovis 10.14, Moniezia expansa 6.39, and Cooperia curticei with 2.21 percent. The intensity ranged from 5 eggs per gram of stool sample as in the case of F. hepatica to 1,500 eggs per gram in T. capricola samples. Moreover, 68.7% of the goats sampled showed concurrent infections of two to eight different parasite species per goat.

scholarly journals Serum Protein Profile Of Iraqi Hydatidosis Patients with Different Sites of Infection

2007 ◽  
Vol 4 (4) ◽  
pp. 612-616
Author(s):  
Baghdad Science Journal
Keyword(s):  
Serum Protein ◽  
Protein Profile ◽  
The Body ◽  
The Other ◽  
Control Group ◽  
Healthy Individuals ◽  
Banding Patterns ◽  
Blood Samples ◽  
Molecular Weights ◽  
Serum Protein Profile

Blood samples of One hundred and twenty patients from different hospitals in Baghdad infected with hydatidosis in different sites of the body (Liver, Lung, multiorgans and kidney) were collected for this study. On the other hand, 30 healthy individuals were included as a control group. This study was conducted to evaluate the effect of this disease on the serum protein profile of the patients using electrophoresis. The results revealed four different protein banding patterns with difference in number of bands and their molecular weights in comparison to the control group, and these differences depended on the site of infection. However the data showed a presence of the same band in all patients with different site of infection.

scholarly journals 380 Effects of estradiol benzoate on reproductive characteristics in beef cows

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 145-145
Author(s):  
Lacey M Dysart ◽  
Caleb O Lemley ◽  
Riley Messman ◽  
Jamie E Larson
Keyword(s):  
Doppler Ultrasonography ◽  
Blood Perfusion ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
The Other ◽  
Control Group ◽  
Blood Samples ◽  
Doppler Probe ◽  
Imagej Software

Abstract Ovulation prompted by exogenous GnRH may cause the release of sub-mature oocytes and lead to decreased pregnancy rates. Inclusion of estradiol benzoate (EB) has improved pregnancy rates. The objective was to determine if EB affects blood perfusion of follicles, CL, or uterus, concentrations of estradiol, or incidence of standing estrus. Forty-seven suckled beef cows were synchronized (GnRH+CIDR on d -9, PGF2a+CIDR removal on d -2, expected estrus=d 0). On d -1, 24 of 47 cows received an injection of EB (1mg/2mL) while the other 23 remained as a control group. Ovaries were evaluated via Doppler ultrasonography on d -1, 0, 6, 14, and 21. A laser Doppler probe was used to measure blood perfusion of the uterus on d -1, 0, and 6. Only cows that exhibited estrus (n = 29) were evaluated on d 14 and 21. Images were analyzed via ImageJ software to determine mean area of perfusion. Blood samples were collected on d -1 and 0 and analyzed for concentrations of estradiol using RIA. Using SAS, MIXED and FREQ were used to determine the influence of treatment on blood perfusion and estradiol, and incidence of standing estrus, respectively. Treatment with EB increased (P < 0.001) the number of cows exhibiting standing estrus compared to control (21/24 vs 8/23, respectively). Treatment of EB did not (P > 0.10) alter blood perfusion of the follicles or uterus on any day or perfusion of the CL on d 6. However, EB increased (P < 0.036) the size of the CL on d 14 (25.06±1.7 vs 6.06±3.7) and tended (P < 0.097) to increase the size of the CL on d 21. Treatment of EB increased (P < 0.003) concentrations of estradiol on d 0 compared to control (21.35±4.49 vs 9.44±4.61 pg/mL). In conclusion, treatment with EB may play a role in changing the CL after ovulation.

The effect of deworming naturally infected heifers on liveweight gain, reproductive performance and fecal egg counts

1993 ◽  
Vol 73 (2) ◽  
pp. 287-293
Author(s):  
S. L. Boyles ◽  
L. J. Johnson ◽  
W. D. Slanger ◽  
B. J. Kreft ◽  
J. D. Kirsch
Keyword(s):  
Key Words ◽  
Reproductive Performance ◽  
Fecal Sample ◽  
Average Daily Gain ◽  
Treated Group ◽  
The Other ◽  
Control Group ◽  
Age At Puberty ◽  
Daily Gain

Twenty-six of 52 heifers with an average liveweight of 318 kg were dewormed by intraruminal injection of oxfendazole and placed in drylot on 31 January. The other 26 animals served as controls. All heifers were pastured starting on 14 June and the treatment heifers were dewormed in the same manner as at the start of the experiment (31 January) on 1 July and 31 July. The groups started out with similar total worm eggs per gram of fecal sample, but the dewormed heifers had fewer counts each month thereafter. The drylot average daily gain of the treated heifers was higher (P = 0.01) and less variable (P = 0.12) and by 29 May these animals were heavier by 11.7 kg (P = 0.10). The treated animals did not gain as fast on pasture (P = 0.20), but the gain continued to be less variable (P = 0.09). Age at puberty was not different. Number of open heifers in the treated group was 4 vs. 15% for the control group (P = 0.18). Deworming by intraruminal injection resulted in faster feedlot gains, more uniform gains during both drylot and pasture, reduced parasites and no abscess at injection sites. Key words: Anthelmintics, oxfendazole, cattle, heifers

Newborn Screening for Hermansky-Pudlak Syndrome Type 3 in Puerto Rico.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3290-3290
Author(s):  
Maribel Torres-Serrant ◽  
Sonia I. Ramirez ◽  
Carmen L. Cadilla ◽  
Maria E. Echevarria ◽  
Pedro J. Santiago
Keyword(s):  
Puerto Rico ◽  
Newborn Screening ◽  
Puerto Rican ◽  
Skin Pigmentation ◽  
The Other ◽  
Diagnosis And Treatment ◽  
Dna Pooling ◽  
Blood Samples ◽  
High Prevalence ◽  
Hermansky Pudlak Syndrome

Abstract Background: Hermansky-Pudlak Syndrome (HPS) is an autosomal recessive disorder characterized by albinism, mucocutaneous bleeding, and storage of ceroid material in macrophages (Hermansky and Pudlak, 1959). Many of these patients develop pulmonary fibrosis and colitis from which about 68% eventually die (Witkop et al, 1990). Patients that are not easily identified by physical characteristics (mostly HPS-3 patients) may have serious hemorrhagic complications when suffer severe injuries or surgical interventions. HPS is a rare disease worldwide, but it is the most common single-gene disorder among persons of Puerto Rican descent (Witkop et al, 1990). Two founder mutations (HPS-1 and HPS-3) account for most HPS cases in Puerto Rico (PR). The first one is widely prevalent in the Northwestern region (Oh et al, 1996) and the other (HPS-3) appeared in a small mid-central region of the island (Anikster et al,2000). HPS-3 patients usually present minimal skin pigmentation deficiency and thus diagnosis of albinism often is missed. Visual acuity problems often are detected late in infancy and childhood. This usually results in poor school progress, late diagnosis and treatment of patients, and delayed counseling of parents. Objective: To determine the prevalence of HPS-3 in Puerto Rican (PR) newborns using DNA pooling technique. Design/Methods: An aleatory sample of 4,690 PR infants born in 2005 (representing approximately 10% of annual PR births) was tested for the HPS-3 mutation, using DNA extracted from dried blood samples (Drocopoli et al, 1996). PCR analysis was carried out as described (Oh et al, 1996; Anikster et al,2000). Samples were tested in DNA pools of 5 newborns each. The validation of the PCR pooling technique for HPS-3 had been carried out in earlier studies in our laboratory by testing 1,500 newborn dried blood samples individually and in 300 total 5-sample pools. All positive samples detected individually were also unequivocally identified as positive when tested in pools. Results: Among the 4,690 newborns tested, 56 presented the HPS-3 mutation and they were confirmed in repeated testing. Two newborns were found to be HPS-3 homozygous. This finding was confirmed several times. The HPS-3 carrier frequency in the island-wide newborn population was 1:84 (1.19%). Both homozygous infants were born close to but outside of the high prevalence region previously reported in PR (Anikster et al,2001). Forty five percent of infants heterozygous for the HPS-3 mutation and one homozygous were found in the high prevalence area and the surrounding 10 miles radius; the other 56% of cases were distributed throughout the rest of the island. Conclusions: Our study has shown that the high prevalence area previously described by Anikster et al, where the founder mutation was identified has been spreading out throughout the rest of the island. Apparently, this is the result of rapid mobility of the Puerto Rican population during the last decades. Our data also demonstrate that the relatively high prevalence of the HPS-3 mutation (1.19%) justifies universal newborn screening. The use of DNA pooling reduces time and labor in newborn screening thus facilitating early diagnosis and treatment of children with HPS-3 and the provision of genetic counseling to patient’s parents and relatives.

scholarly journals The Uptake of Radio-Active Phosphate by Nematode Parasites and by Tissues of the Sheep

1951 ◽  
Vol 4 (4) ◽  
pp. 575
Author(s):  
Helene B Esserman ◽  
Pauline M Sambell
Keyword(s):  
Haemonchus Contortus ◽  
Nematode Parasites ◽  
Oesophagostomum Columbianum ◽  
Relative Uptake

The relative uptake of radio-active phosphate by the tissues of the sheep and by the parasites Trichostrongylus spp., Haemonchus contortus, and Oesophagostomum columbianum has been investigated.

PRELIMINARY NOTE ON THE ADMINISTRATION OF NON-CONDITIONED PHENOTHIAZINE, IN SMALL DAILY DOSES, FOR THE CONTROL OF GASTROINTESTINAL PARASITES OF CATTLE IN PUERTO RICO

1969 ◽  
Vol 27 (3) ◽  
pp. 125-130
Author(s):  
John S. Andrews ◽  
Carlos M. Muñiz ◽  
Felix Orihuela
Keyword(s):  
Puerto Rico ◽  
Dose Rate ◽  
Haemonchus Contortus ◽  
Live Weight ◽  
Gastrointestinal Parasites ◽  
Preliminary Note ◽  
Nematode Larvae ◽  
Small Intestinal

The results of the foregoing experiments show that daily doses of 1/2 gram of nonconditioned phenothiazine per 100 pounds of live weight administered to cattle infected with gastrointestinal parasites reduced the number of worm eggs passed unto the pasture by the infected animals. The eggs of the stomach worms, Haemonchus contortus and H. similis, the small intestinal hair-worms, Trichostrongylus spp., the hookworm, Bunostomum phlebotomum, and the nodular worm, Oesophagostomum radiatum disappear relatively quickly from the feces of the treated animals. The eggs of Cooperia spp., Strongyloides papillosus, and the broad tapeworms, Moniezia spp., were not markedly reduced in number by this treatment. These results confirm those of Porter, Simms, and Cauthen (1941) showing that phenothiazine has little effect on the last three parasites mentioned. The dose rate used in these experiments compares favorably with that suggested by Shorb and Habermann (loc. cit.) for the prevention of the development of nematode larvae in the feces of sheep.

scholarly journals Effect of different doses of uranyl acetate on some blood parameters and hepatic enzymesin females rats.

2014 ◽  
Vol 8 (3) ◽  
pp. 37-43
Author(s):  
Sarah Ghazi Alzorii
Keyword(s):  
Oral Administration ◽  
Liver Enzyme ◽  
Blood Parameters ◽  
Uranyl Acetate ◽  
The Other ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Blood Samples ◽  
Different Doses

This study was carried out to investigate effects of oral administration of uranyl acetate on haematological parameters and liver enzyme. For this purpose, twenty females mature Albino rats were divided into four equal groups, control group G1 administrated distilled water, the other three groups administrated orally 50,75,100 mg/kg/b.w. /day of uranyl acetate. The route of administration was oral intubations for 10 weeks, for 14 days before mating with untreated males, as well as during pregnancy 3 weeks and lactation 5 weeks. At the end of the treatment blood samples were collected from mothers of rats. The results were obtained a significant P< 0.05 increase in the total WBC counts in groups G2,G3,G4 compared with control .A significant P<0.05 decrease in the lymphocytes and monocytes percentage in groups (G3,G4), while no significant decrease P>0.05 in G2 in the lymphocytes and monocytes percentage compared with control. And the results show significant P< 0.05 decrease in HB value in groups G3,G4, while no significant decrease P>0.05 in G2 in HB value compared with control. A significant P< 0.05 decrease was observed in total RBCs counts in groups G2,G3,G4 compared with control. No significant increase P>0.05 in GOT and GPT in groups G2 and G3 and significant P<0.05 increase in GOT and GPT activity in the serum in group G4 compared with control. No significant increase P>0.05 in CPK activity in groups G2,G3,G4 compared with control.

Effect of Fibrinolytic Microbes and Enzymes on Biochemical Blood Parameters in Crossbred Calves

2019 ◽  
Vol 15 (02) ◽  
pp. 18-21
Author(s):  
R I Kapadiya ◽  
S V Shah ◽  
Y G Patel ◽  
P R Pandya
Keyword(s):  
Blood Parameters ◽  
The Other ◽  
Control Group ◽  
Glutamic Pyruvic Transaminase ◽  
Normal Range ◽  
Blood Samples ◽  
Overall Performance ◽  
Similar Body ◽  
Control Plasma ◽  
Plasma Phosphorus

Twenty crossbred (HF x Kankrej) calves were allotted randomly to five groups (each had one male and three female calves) almost with similar body weight (85.70±6.37 kg) and age (167.55±21.70 days). Experimental calves of the control group were offered hybrid napier untreated, and the other four groups were offered hybrid napier treated with (i) fungus- Aspergillus spp. (1×107 per g feed), (ii) fibrolytic bacteria- Escherichia spp. (106 CFU per g feed), (iii) xylanase (50 mL/kg having xylanase 1.2 IU/mL), and (iv) consortium of 1/3rd dose of fungus + bacteria + enzyme as treatments for 140 days. The blood samples were collected from each calf at a biweekly intervals in the EDTA vacuttee. Nutrients offered to crossbred calves were sufficient to satisfy the nutrients requirements. Bacteria fed calves had significantly higher (plessthan0.05) mean hemoglobin levels than control. Blood plasma glucose of calves in fibrolytic microbes and enzyme groups was statistically similar to control. Feeding of fungus, bacteria, enzyme, and consortium was without a significant effect on plasma total proteins.In comparison to control (7.53 mg/dL), the plasma phosphorus was significantly (plessthan0 0.05) higher in calves fed enzyme (7.87 ± 0.20 mg/dl) and lower in group fed fungus (7.13 mg/dL). In contrast, in bacteria (7.57 mg/dL) and consortium group (7.60 mg/dL) it was statistically similar to control. Plasma glutamic pyruvic transaminase concentration of crossbred calves was lower ( pless than 0.05) in enzyme and higher (p lessthan 0.05) in fungus, bacteria, and consortium groups than in control, while plasma glutamic oxaloacetate transaminase concentration in all groups was statistically similar. In conclusion, all blood parameters of different groups were within normal range and without adverse effect on the overall performance of calves.

scholarly journals Comparison of four methods of inducing pseudopregnancy in rabbits

2022 ◽  
Vol 78 (01) ◽  
pp. 6608-2022
Author(s):  
SYAFRUDDIN SYAFRUDDIN ◽  
SRI WAHYUNI ◽  
GHOLIB GHOLIB ◽  
TONGKU NIZWAN SIREGAR
Keyword(s):  
Positive Control ◽  
The Other ◽  
Control Group ◽  
Corpora Lutea ◽  
Histological Observation ◽  
Blood Samples ◽  
Treatment Groups ◽  
Examination Time ◽  
Significant Difference ◽  
The Difference

This study was conducted to compare several methods for inducing pseudopregnancy in local rabbits. The study used 25 clinically healthy rabbits of local breeds, aged 1-1.5 years, with a bodyweight of 1.8-2.2 kg. The rabbits were divided into five treatment groups (n = 5): R1, R2, R3, R4, and R5. Each group was given a different treatment. Group R1 was injected intramuscularly with 100 IU PMSG and mated with males three days later, which was followed by an intravenous injection of 75 IU hCG. Pseudopregnancy in group R2 was induced through artificial copulation by inserting a 1 cm long cotton bud into vaginal lumen at 5 a.m. Pseudopregnancy in group R3 was induced using 50 IU hCG intramuscularly. Group R4 was injected intravenously with 5 g GnRH and then intramuscularly with 100 IU PMSG and three days later with 75 IU hCG. Blood samples were collected on days 2, 4, 6, and 8 for estrogen and progesterone measurement, whereas an ovarian histological observation of all groups was performed on day 8. The results show that the highest concentration of estrogen was obtained in group R2, which was significantly different from the other groups (P <0.05), but the difference in examination time was not statistically significant (P > 0.05). Furthermore, the concentration of progesterone in all groups fluctuated at various times of the day. Progesterone concentrations were higher in group R5, in which pseudopregnancy was induced with PMSG and hCG. Group R5 showed a significant difference (P <0.05) with R2, R3, and R4, but the difference with the positive control group (R1) was not significant (P > 0.05). The numbers of corpora lutea in groups R1, R2, R3, R4, and R5 were, respectively, 6.87 ± 1.58, 6.22 ± 2.71, 6.74 ± 1.94, 5.98 ± 2.04, and 8.8 ± 1.90, and the largest diameter was obtained in R5 (1.65 ± 0.37 mm). In conclusion, the best method of inducing pseudopregnancy in local rabbits is the administration of PMSG and hCG.

Sign in / Sign up

Export Citation Format

Share Document