Modulation of the atrial specific Kv1.5 channel by the n-3 polyunsaturated fatty acid, α-linolenic acid

2008 ◽  
Vol 44 (2) ◽  
pp. 323-335 ◽  
Author(s):  
Miriam Guizy ◽  
Miren David ◽  
Cristina Arias ◽  
Lian Zhang ◽  
Montserrat Cofán ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linolenic Acid ◽  
Polyunsaturated Fatty Acid

scholarly journals The impact of plasma membrane lipid composition on flagella-mediated adhesion of enterohemorrhagic Escherichia coli

2020 ◽  
Author(s):  
Hélène Cazzola ◽  
Laurine Lemaire ◽  
Sébastien Acket ◽  
Elise Prost ◽  
Luminita Duma ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Lipid Rafts ◽  
Cell Lines ◽  
Polyunsaturated Fatty Acid ◽  
Bacterial Adhesion ◽  
Human Pathogen ◽  
The Impact

AbstractEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is a major cause of foodborne gastrointestinal illness. The adhesion of EHEC on host tissues is the first step enabling bacterial colonization. Adhesins like fimbriae and flagella mediate this mechanism. Here, we studied the interaction of the bacterial flagellum with the host cell’s plasma membrane using Giant Unilamellar Vesicles (GUVs) as a biologically relevant model. Cultured cell lines contain many different molecular components including proteins and glycoproteins. In contrast, with GUVs we can characterize the bacterial mode of interaction solely with a defined lipid part of the cell membrane. Bacterial adhesion on GUVs was dependent on the presence of the flagellar filament and its motility. By testing different phospholipid head groups, the nature of the fatty acid chains or the liposome curvature, we found that lipid packing is a key parameter to enable bacterial adhesion. Using HT-29 cells grown in the presence of polyunsaturated fatty acid (α-linolenic acid) or saturated fatty acid (palmitic acid), we found that α-linolenic acid reduced adhesion of wild type EHEC but not of a non-flagellated mutant. Finally, our results reveal that the presence of flagella is advantageous for the bacteria to bind to lipid rafts. We speculate that polyunsaturated fatty acids prevent flagellar adhesion on membrane bilayers and play a clear role for optimal host colonization. Flagella-mediated adhesion to plasma membranes has broad implications to host-pathogen interactions.ImportanceBacterial adhesion is a crucial step to allow bacteria to colonize their hosts, invade tissues and form biofilm. Enterohemorrhagic E. coli O157:H7 is a human pathogen and the causative agent of diarrhea and hemorrhagic colitis. Here, we use biomimetic membrane models and cell lines to decipher the impact of lipid content of the plasma membrane on enterohemorrhagic E. coli flagella-mediated adhesion. Our findings provide evidence that polyunsaturated fatty acid (α-linolenic acid) inhibits E. coli flagella adhesion to the plasma membrane in a mechanism separate from its antimicrobial and anti-inflammatory functions. In addition, we confirm that cholesterol-enriched lipid microdomains, often called lipid rafts are important in bacterial adhesion. These findings significantly strengthen plasma membrane adhesion via bacterial flagella in an important human pathogen. This mechanism represents a promising target for the development of novel anti-adhesion therapies.

scholarly journals Substitution of linoleic acid with α-linolenic acid or long chain n-3 polyunsaturated fatty acid prevents Western diet induced nonalcoholic steatohepatitis

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Sugeedha Jeyapal ◽  
Suryam Reddy Kona ◽  
Surekha Venkata Mullapudi ◽  
Uday Kumar Putcha ◽  
Puvaneswari Gurumurthy ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Polyunsaturated Fatty Acid ◽  
Nonalcoholic Steatohepatitis ◽  
Western Diet ◽  
Long Chain

scholarly journals Inhibitory Effect of Dietary Perilla Oil Rich in the n-3 Polyunsaturated Fatty Acid α-Linolenic Acid on Colon Carcinogenesis in Rats

1991 ◽  
Vol 82 (10) ◽  
pp. 1089-1096 ◽  
Author(s):  
Tomio Narisawa ◽  
Masahiro Takahashi ◽  
Hitoshi Kotanagi ◽  
Hisashi Kusaka ◽  
Yoshihiko Yamazaki ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linolenic Acid ◽  
Polyunsaturated Fatty Acid ◽  
Colon Carcinogenesis ◽  
Inhibitory Effect ◽  
Perilla Oil

scholarly journals Serine/threonine Kinases Play Important Roles in Regulating Polyunsaturated Fatty Acid Biosynthesis in Synechocystis sp. PCC6803

2021 ◽  
Vol 9 ◽  
Author(s):  
Gao Chen ◽  
Yuelei Cao ◽  
Huairong Zhong ◽  
Xiaodong Wang ◽  
Yanle Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linolenic Acid ◽  
Polyunsaturated Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Gene Knockout ◽  
Acid Biosynthesis ◽  
Fatty Acid Desaturases ◽  
Unicellular Cyanobacterium ◽  
Knockout Mutants ◽  
Threonine Kinases

Serine/threonine kinases (STKs) play important roles in prokaryotic cellular functions such as growth, differentiation, and secondary metabolism. When the external environment changes, prokaryotes rely on signal transduction systems, including STKs that quickly sense these changes and alter gene expression to induce the appropriate metabolic changes. In this study, we examined the roles of the STK genes spkD and spkG in fatty acid biosynthesis in the unicellular cyanobacterium Synechocystis sp. PCC6803, using targeted gene knockout. The linoleic acid (C18: 2), γ-linolenic acid (C18: 3n6), α-linolenic acid (C18: 3n3), and stearidonic acid (C18: 4) levels were significantly lower in spkD and spkG gene knockout mutants than in the wild type at a culture temperature of 30°C and a light intensity of 40 μmol⋅m–2⋅s–1. The expression levels of fatty acid desaturases and STK genes differed between the spkD and spkG gene knockout mutants. These observations suggest that spkD and spkG may directly or indirectly affect the fatty acid composition in Synechocystis sp. PCC6803 by regulating the expression of fatty acid desaturases genes. Therefore, the STK genes spkD and spkG play important roles in polyunsaturated fatty acid biosynthesis in Synechocystis sp. PCC6803. These findings could facilitate the development of cyanobacteria germplasm resources that yield high levels of fatty acids. In addition, they provide a theoretical basis for the genetic engineering of cyanobacteria with improved yields of secondary metabolites and increased economic benefits.

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