Su1801 – A Novel Salt of Resolvin E1 Controls Disease Activity in the Dextran Sulfate Sodium Colitis Mouse Model and Reduces Inflammation in Colon Tissue Explants from Ulcerative Colitis Patients

Plant-sourced formulations such as Iberogast and the traditional Chinese medicine formulation, Cmed, purportedly possess anti-inflammatory and radical scavenging properties. We investigated Iberogast and Cmed, independently, for their potential to decrease the severity of the large bowel inflammatory disorder, ulcerative colitis. Sprague Dawley rats (n = 8/group) received daily 1 mL gavages (days 0-13) of water, Iberogast (100 μL/200 μL), or Cmed (10 mg/20 mg). Rats ingested 2% dextran sulfate sodium or water ad libitum for 7 days commencing on day 5. Dextran sulfate sodium administration increased disease activity index scores from days 6 to 12, compared with water controls ( P < .05). On day 10, 200 μL Iberogast decreased disease activity index scores in colitic rats compared with colitic controls ( P < .05). Neither Iberogast nor Cmed achieved statistical significance for daily metabolic parameters or colonic crypt depth. The therapeutic effects of Iberogast and Cmed were minimal in the colitis setting. Further studies of plant extracts are required investigating greater concentrations and alternative delivery systems.

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Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium

BMC Complementary and Alternative Medicine ◽

10.1186/s12906-019-2750-y ◽

2019 ◽

Vol 19 (1) ◽

Author(s):

Ling-yan Pei ◽

Yu-shi Ke ◽

Huan-hu Zhao ◽

Wei-zhi Liu ◽

Lin Wang ◽

...

Keyword(s):

Ulcerative Colitis ◽

Mouse Model ◽

Gut Microbiota ◽

Therapeutic Effect ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Control Group ◽

Group Model ◽

Regulatory Effect ◽

Model Group

Abstract Background Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern. Studies have shown that UC is closely associated with the gut microbiota. Garidisan is composed of wild poppy and Artemisia frigida Willd and is commonly used for the treatment of UC in Inner Mongolia, China. In clinical settings, Garidisan has been found to treat UC effectively, with low recurrence. Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear. In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC. Methods The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions. High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed. The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis. Results The UC mouse model was successfully established and the sequencing results were of adequate quality. There were significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group, and the differences between groups were greater than those within any group. The therapeutic effect of Garidisan on UC is attributed to the direct effect on the Lachnospiraceae family of bacteria. Conclusion Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.

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Lactobacillus fermentum ZS40 Ameliorates Inflammation in Mice With Ulcerative Colitis Induced by Dextran Sulfate Sodium

Frontiers in Pharmacology ◽

10.3389/fphar.2021.700217 ◽

2021 ◽

Vol 12 ◽

Author(s):

Zixia Chen ◽

Long Yi ◽

Yanni Pan ◽

Xingyao Long ◽

Jianfei Mu ◽

...

Keyword(s):

Ulcerative Colitis ◽

Inflammatory Cytokines ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Intestinal Flora ◽

Lactobacillus Fermentum ◽

Mouse Serum ◽

Colon Tissue ◽

Relative Expression ◽

Anti Inflammatory

Ulcerative colitis is an inflammatory disease of the intestine caused by many reasons, and it may even develop into colon cancer. Probiotics are normal bacteria that exist in the human body and have been proven to regulate the balance of intestinal flora and alleviate inflammation. The current study aimed to study the effect of Lactobacillus fermentum ZS40 (ZS40) on dextran sulfate sodium (DSS)-induced ulcerative colitis mice. The length and weight of the colon were measured, and the histopathological morphological changes of colon tissue were observed to evaluate the effects of ZS40 on colitis. Biochemical kits, ELISA kits, real-time quantitative PCR (RT-qPCR), and western blot were also used to detect the effects of ZS40 on serum and colon tissue related oxidative indicators and pro-inflammatory and anti-inflammatory cytokines. We found that ZS40 could reduce colonic inflammatory cell infiltration and goblet cell necrosis, increase total superoxide dismutase and catalase in mouse serum, and reduce myeloperoxidase and malondialdehyde levels. ZS40 could down-regulate the level of proinflammatory cytokines and up-regulate the level of anti-inflammatory cytokines. More importantly, ZS40 down-regulated the relative expression of nuclear factor-κB p65 (NF-κBp65), IL-6, and TNF-α mRNA and protein, up-regulated the relative expression of inhibitor kapa B alpha (IκB-α). By regulating the NF-κB and MAPK pathways to down-regulated the relative expression of p38 and JNK1/2 mRNA and p38, p-p38, JNK1/2, and p-JNK1/2 proteins. Our study suggested that ZS40 may serve as a potential therapeutical strategy for ulcerative colitis.

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Characteristics of Colon Crypt Stem Cells in Preserved Epithelial Cell Clumps in Dextran Sulfate Sodium-induced Mouse Model of Ulcerative Colitis

10.21203/rs.3.rs-144494/v1 ◽

2021 ◽

Author(s):

Mio Kobayashi ◽

Risako Yamashita ◽

Ryo Ichikawa ◽

Makoto Shibutani ◽

Toshinori Yoshida

Keyword(s):

Ulcerative Colitis ◽

Stem Cells ◽

Stem Cell ◽

Mouse Model ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Stem Cell Marker ◽

Stem Cell Markers ◽

Cell Marker ◽

Cell Markers

Abstract Crypt stem cells rescue the colorectum from refractory ulcers in ulcerative colitis (UC). We previously reported that clumps of a few epithelial cells were scattered in ulcer regions in a dextran sulfate sodium (DSS)-induced mouse model of UC; however, the origin of the clumps is unknown. We determined the immunohistochemical expression of stem-cell markers in the epithelial clumps in female Balb/c mice administered DSS in drinking water for 6 days, followed by withdrawal of DSS for 6 days. Similar to the characteristics of UC, the ulcers were more severe in the distal region close to the anus than in the proximal region of the colorectum. Quantitative analyses revealed that the epithelial clumps appeared in relation to the severity of ulcer, and they expressed the cell adhesion molecules E-cadherin and β-catenin. Among stem cell markers, the epithelial clumps primarily expressed + 5 cell marker Dll1, followed by + 4 cell marker Bmi1 and crypt stem cell marker Lgr5 in that order. Nuclear expression of Sox9, but not Ki-67 and β-catenin was identified in the clumps. The present results suggest that the epithelial clumps comprised crypt-derived stem cells with the potential to regenerate crypts, which could serve as a potential treatment strategy for UC.

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Ultraperformance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolomics and Lipidomics Identify Biomarkers for Efficacy Evaluation of Mesalazine in a Dextran Sulfate Sodium-Induced Ulcerative Colitis Mouse Model

Journal of Proteome Research ◽

10.1021/acs.jproteome.0c00757 ◽

2020 ◽

Author(s):

Kun Liu ◽

Bingjie Jia ◽

Liping Zhou ◽

Lei Xing ◽

Lvying Wu ◽

...

Keyword(s):

Mass Spectrometry ◽

Ulcerative Colitis ◽

Liquid Chromatography ◽

Mouse Model ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Time Of Flight ◽

Efficacy Evaluation ◽

Flight Mass Spectrometry

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Involvement of kallikrein-kinin system in development of experimental ulcerative colitis induced by dextran sulfate sodium in rats

Gastroenterology ◽

10.1016/s0016-5085(01)83453-3 ◽

2001 ◽

Vol 120 (5) ◽

pp. A694-A694

Author(s):

K KAMATA ◽

I HAYASHI ◽

K BOKU ◽

T SAEKI ◽

T OHNO ◽

...

Keyword(s):

Ulcerative Colitis ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Kinin System ◽

Kallikrein Kinin System ◽

Experimental Ulcerative Colitis

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Shikonin inhibits colitis-associated colorectal dysplasias in a mouse model of azoxymethane/dextran sulfate sodium colitis

Planta Medica ◽

10.1055/s-0032-1320447 ◽

2012 ◽

Vol 78 (11) ◽

Author(s):

JL Ríos ◽

A Martí ◽

I Andújar ◽

RM Giner ◽

MC Recio

Keyword(s):

Mouse Model ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium

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Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model

Microorganisms ◽

10.3390/microorganisms9020370 ◽

2021 ◽

Vol 9 (2) ◽

pp. 370

Author(s):

Hyunjoon Park ◽

Soyoung Yeo ◽

Seokwon Kang ◽

Chul Sung Huh

Keyword(s):

Mouse Model ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Intestinal Bleeding ◽

Cross Sectional ◽

Microbiome Analysis ◽

Inflammatory Bowel ◽

Factor Design ◽

The Individual

The role of the gut microbiota in the pathogenesis of inflammatory bowel disease (IBD) has been in focus for decades. Although metagenomic observations in patients/animal colitis models have been attempted, the microbiome results were still indefinite and broad taxonomic presumptions were made due to the cross-sectional studies. Herein, we conducted a longitudinal microbiome analysis in a dextran sulfate sodium (DSS)-induced colitis mouse model with a two-factor design based on serial DSS dose (0, 1, 2, and 3%) and duration for 12 days, and four mice from each group were sacrificed at two-day intervals. During the colitis development, a transition of the cecal microbial diversity from the normal state to dysbiosis and dynamic changes of the populations were observed. We identified genera that significantly induced or depleted depending on DSS exposure, and confirmed the correlations of the individual taxa to the colitis severity indicated by inflammatory biomarkers (intestinal bleeding and neutrophil-derived indicators). Of note, each taxonomic population showed its own susceptibility to the changing colitis status. Our findings suggest that an understanding of the individual susceptibility to colitis conditions may contribute to identifying the role of the gut microbes in the pathogenesis of IBD.

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Oat β-glucan ameliorates dextran sulfate sodium (DSS)-induced ulcerative colitis in mice

Food & Function ◽

10.1039/c5fo00563a ◽

2015 ◽

Vol 6 (11) ◽

pp. 3454-3463 ◽

Cited By ~ 42

Author(s):

Bo Liu ◽

Qinlu Lin ◽

Tao Yang ◽

Linna Zeng ◽

Limin Shi ◽

...

Keyword(s):

Ulcerative Colitis ◽

Oral Administration ◽

Inflammatory Cytokines ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Tnf Α

Oral administration of oat β-glucan ameliorates DSS induced colitis in mice by decreasing the expression of inflammatory cytokines TNF-α, IL-1β, IL-6 and iNOS.

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