57: Metabonic Approach for the Diagnosis of Urinary Tract Infection Caused by Common Uropathogens (E. coli, Pseudomonas Aeruginosa, Klebsiella Pneumoniae): 1H NMR Spectroscopic Study

Abstract Objectives To assess the impact of ESBL production on mortality and length of hospital stay (LOS) of community-onset infections due to Escherichia coli or Klebsiella pneumoniae. Methods A population-based cohort study including all adult patients hospitalized with a first-time community-onset E. coli or K. pneumoniae bacteraemia or urinary tract infection in the North Denmark Region between 2007 and 2017. For each bacterial agent, we computed 1 year Kaplan–Meier survival curves and cumulative incidence functions of LOS, and by use of Cox proportional hazard regression we computed HRs as estimates of 30 day and 1 year mortality rate ratios (MRRs) and LOS among patients with and without ESBL-producing infections. Results We included 24 518 cases (among 22350 unique patients), of whom 1018 (4.2%) were infected by an ESBL-producing bacterium. The 30 day cumulative mortality and adjusted MRR (aMRR) in patients with and without ESBL-producing isolates was as follows: E. coli bacteraemia (n = 3831), 15.8% versus 14.0%, aMRR = 1.01 (95% CI = 0.70–1.45); E. coli urinary tract infection (n = 17151), 9.5% versus 8.7%, aMRR = 0.97 (95% CI = 0.75–1.26); K. pneumoniae bacteraemia (n = 734), 0% versus 17.2%, aMRR = not applicable; and K. pneumoniae urinary tract infection (n = 2802), 13.8% versus 10.7%, aMRR = 1.13 (95% CI = 0.73–1.75). The 1 year aMRR remained roughly unchanged. ESBL-producing E. coli bacteraemia was associated with an increased LOS compared with non-ESBL production. Conclusions ESBL production was not associated with an increased short- or long-term mortality in community-onset infections due to E. coli or K. pneumoniae, yet ESBL-producing E. coli bacteraemia was associated with an increased LOS.

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1H NMR spectroscopy-based metabolomics analysis for the diagnosis of symptomatic E. coli-associated urinary tract infection (UTI)

BMC Microbiology ◽

10.1186/s12866-017-1108-1 ◽

2017 ◽

Vol 17 (1) ◽

Cited By ~ 5

Author(s):

Milena Lussu ◽

Tania Camboni ◽

Cristina Piras ◽

Corrado Serra ◽

Francesco Del Carratore ◽

...

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Nmr Spectroscopy ◽

Tract Infection ◽

1H Nmr ◽

1H Nmr Spectroscopy ◽

E Coli ◽

Metabolomics Analysis

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Multiple-Antibiotic Resistance Mediated by Plasmids and Integrons in Uropathogenic Escherichia coli and Klebsiella pneumoniae

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v24i1.1231 ◽

1970 ◽

Vol 24 (1) ◽

pp. 19-23 ◽

Cited By ~ 7

Author(s):

Taslima Taher Lina ◽

Sabita Rezwana Rahman ◽

Donald James Gomes

Keyword(s):

Escherichia Coli ◽

Drug Resistance ◽

Urinary Tract Infection ◽

Antibiotic Resistance ◽

Urinary Tract ◽

Tract Infection ◽

Klebsiella Pneumoniae ◽

High Rate ◽

E Coli ◽

Class 1

Antibiotic resistance in urinary tract infection (UTI) is a growing public health problem in the world. In this study, a total of 182 uropathogens were isolated from patients with symptoms of urinary tract infection (UTI). Escherichia coli (88%) was the most prevalent isolate, while Klebsiella pneumoniae was recovered from 12% cases. The male/female ratio was 1:3. About 56% female and 51% male patients belonged to the age group >40 years. The antibiotic resistance rates of the isolates to fifteen different drugs were investigated. E. coli and K. pneumoniae showed variable pattern of susceptibility. The percentage of resistance to different drugs was higher in E. coli isolates compared to that of K. pneumoniae. Among the total number of isolates about 87% were resistant to at least three commonly used antibiotics. All the isolates were sensitive to imipenem. Analysis of the plasmid DNA had shown that the plasmid pattern was very diverse in both E. coli and K. pneumoniae. All the isolates contained multiple numbers of plasmid ranging from 1.0 to >140 MDa. Middleranged plasmids (30 to 80 MDa), the transferable resistance plasmids, were found to be present in 86% E. coli and 85% K. pneumoniae isolates. The strong association observed between plasmid profiles and drug resistance patterns suggest that plasmids other than the common plasmids may have epidemiological significance. The presence of class 1 and class 2 integrons were also investigated. A relatively high occurrence of class 1 integrons, that are associated with lateral transfer of antibacterial resistance genes, was observed in K. pneumoniae (88%) than in E. coli isolates (54%). Class 2 integrons were not found in any of the E. coli and K. pneumoniae isolates. These results show the high rate of drug resistance and the presence of high rate of transferable elements in these MDR isolates. Keywords: Uropathogens, Escherichia coli, Klebsiella pneumoniae, Multidrug-resistant (MDR) bacteria, Plasmid profiles, IntegronsDOI: http://dx.doi.org/10.3329/bjm.v24i1.1231 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 19-23

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Prevalence of Urinary Tract Infection among Adult Females in Omu-Aran South-West Nigeria

South Asian Journal of Research in Microbiology ◽

10.9734/sajrm/2020/v6i130140 ◽

2020 ◽

pp. 16-24

Author(s):

Ndako James A. ◽

Akinyomade Owolabi ◽

Oludolapo Olatinsu ◽

Adedapo O. Omolade ◽

Ilochi Ifeanyi

Keyword(s):

Escherichia Coli ◽

Pseudomonas Aeruginosa ◽

Urinary Tract Infection ◽

Candida Albicans ◽

Urinary Tract ◽

Tract Infection ◽

Klebsiella Pneumoniae ◽

Pregnant Women ◽

Bacterial Growth ◽

Proteus Species

Background: Urinary tract infection is caused by the presence and replication of microorganisms in the urinary tract. Urinary tract infection is a major health problem among females especially among pregnant women and this is caused by the physiological makeup of the female urinary tract. Objective: This study was designed to assess the epidemiology of UTIs present amidst pregnant women and females residing in the Omu-Aran community. Materials and Methods: A total of 100 individuals were involved of which 50 were pregnant women while the remaining 50 were non-pregnant females. Semi-structured questionnaires were distributed to all participants to obtain their data. Samples of urine (early morning midstream) were collected in universal bottles from participants. The urine specimens were cultured on four different agar plates which were; cystein lactose electrolyte deficient agar, blood agar, MacConkey agar and nutrient agar for significant bacteria growth. Microscopic and macroscopic examination was also carried out on the samples for possible detection of infections. Results: The result of the culture showed a significant bacterial growth of 77% and 23% of the samples collected showed no significant bacterial growth. Bacteria such as Escherichia coli, Klebsiella pneumoniae, Staphylococcus spp., Proteus species, Pseudomonas aeruginosa and Candida albicans were isolated. With Staphylococcus aureus predominantly present and observed as the causative factor for (45.4%) occurrence of UTIs, closely followed by Staphylococcus saprophyticus (13.0%), Escherichia coli (13.0%), Klebsiella pneumoniae (9.1%) Proteus species (9.1%), Staphylococcus epidermidis (7.8%) and Pseudomonas aeruginosa (1.3%). However the only fungi isolated was Candida albicans showing (1.3%) prevalence, Candida is the most common cause of fungal infections in humans. Conclusion: Results obtained in this work showed a high prevalence of Urinary tract infection at our study location. A prompt enlightenment campaign and need for periodic check among females is highly advocated to reduce or out rightly eliminate the spread of common uropathogens in circulation.

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Occurrence of Extended Spectrum b-lactamases among E. coli and Klebsiella pneumoniae Isolated from Urinary Tract Infection from Tertiary Health Care Center in Amravati District

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/949 ◽

2011 ◽

Vol 8 (2) ◽

pp. 849-852

Author(s):

Niraj Ghanwate ◽

P.V. Thakare ◽

P.R. Bhise ◽

A. Wandhre

Keyword(s):

Health Care ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Klebsiella Pneumoniae ◽

Care Center ◽

Health Care Center ◽

E Coli ◽

Extended Spectrum

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Escherichia coli and Klebsiella pneumoniae Sensitivity/Resistance Pattern Towards Antimicrobial Agents in Primary and Simple Urinary Tract Infection Patients Visiting University Hospital of Jamia Hamdard New Delhi

Drug Research ◽

10.1055/a-0576-0079 ◽

2018 ◽

Vol 68 (07) ◽

pp. 415-420 ◽

Cited By ~ 3

Author(s):

Mohd Rizwan ◽

Mohd Akhtar ◽

Abul Najmi ◽

Kuldeep Singh

Keyword(s):

Escherichia Coli ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Klebsiella Pneumoniae ◽

High Resistance ◽

Resistance Pattern ◽

Age Group ◽

E Coli ◽

Number Of Patients

Abstract Aim This study was carried out to record the sensitivity/resistance pattern of Escherichia coli and Klebsiella pneumoniae towards antimicrobial drugs prescribed to urinary tract infection (UTI) patients. Materials and Methods A total of 68 bacteriologically proven simple UTI patients were included in the study, based on inclusion and exclusion criteria. Subjects demographics, antibiotics prescribed, sensitivity pattern of antibiotics towards the urinary pathogens were recorded in drug utilization and documentation form. Results The mean age of the patients was 29.32 years. The maximum number of patients fell under the age group 21–30 years. The maximum no of male and female patients belonged to the age group 21–30 years, 82.35% were females and 17.64% were males. E. coli found to be the most common uropathogens followed by Klebsiella pneumoniae. Nitrofurantoin was the most commonly prescribed drug followed by ciprofloxacin, levofloxacin and amoxicillin + clavulanic acid and amikacin. E. coli isolates showed high resistance for ampicillin followed by cotrimoxazole, norfloxacin, ciprofloxacin, gentamicin, tetracycline and ceftazidime and least resistance to amikacin, nitrofurantoin. Klebsiella pneumoniae isolates were highly resistant against ceftazidime, ampicilline, cotrimoxazole, nitrofurantoin, gentamicin, amoxicillin + sulbactam. Overall, the antibiotic against with high resistance found was ampicillin followed by cotrimoxazole, gentamicin, ceftazidime, norfloxacin, ciprofloxacin, tetracycline, levofloxacin, cefepime and least resistance to amikacin, netilmicin and nitrofurantoin. Conclusion Majority of UTIs in this study were caused by E. coli followed by Klebsiella pneumoniae. The high resistance was found with ampicillin (75%). Nitrofurantoin was the most commonly prescribed drug followed by other flouroquinolones.

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Investigation on the Effect of Antibiotic and Multivitamin in the Formation of Biofilm in Urinary Tract Infection (UTI) Causing Pathogens

International Journal of Engineering & Technology ◽

10.14419/ijet.v7i4.14.27478 ◽

2019 ◽

Vol 7 (4.14) ◽

pp. 91

Author(s):

K P Sajna ◽

P Paulraj ◽

V Balasupramaniam ◽

P Sajeesh ◽

M Chandramohan ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Klebsiella Pneumoniae ◽

The Body ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Biofilm Growth ◽

Significant Difference

Urinary catheters make humans vulnerable to Urinary Tract Infection (UTI) by damaging the natural barrier of the body. Bacteria which are commonly related to this infection are Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. In this study Klebsiella pneumoniae and Pseudomonas aeruginosa were obtained from the hospital. Levofloxacin is a newly developed fluoroquinolone antibiotic, which is commonly used in clinical practice. The antibacterial effect of levofloxacin was studied using disk diffusion method. In this method the diameter of zone of inhibition in the presence of multivitamin was smaller than without multivitamin. The statistical analysis showed a significant difference in antibiotic sensitivity with and without multivitamin (p<0.05). In the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), It was observed that in the absence of multivitamin Klebsiella pneumoniae growth stopped at the concentration of 200 µg/ml and in the presence of multivitamin the growth stopped at the concentration of 400 µg/ml. The growth of Pseudomonas aeruginosa stopped at the concentration of 400 µg/ml in the absence of multivitamin and in the presence the growth was stopped at the concentration of 800 µg/ml. Similar effect was studied in biofilm form as well by measuring the absorbance at 600nm. Using the absorbance values the biofilm growth curve was carried out and in the presence of multivitamin, both bacteria in single and consortia form stayed in stationary phase longer than without multivitamin. These findings demonstrate that a higher concentration of antibiotic is required to inhibit the growth of bacteria when supplemented with multivitamins and in turn increases the development of antibiotic resistance under biofilm condition.

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EXTENDED-SPECTRUM BETA-LACTAMASE-PRODUCING ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE ISOLATES FROM THE URINE OF DOGS AND CATS SUSPECTED OF URINARY TRACT INFECTION IN A VETERINARY TEACHING HOSPITAL

Taiwan Veterinary Journal ◽

10.1142/s1682648515500274 ◽

2016 ◽

Vol 42 (03) ◽

pp. 143-148 ◽

Cited By ~ 6

Author(s):

Nan-Ling Kuan ◽

Chia-Wei Chang ◽

Chien-An Lee ◽

Kuang-Sheng Yeh

Keyword(s):

Escherichia Coli ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Klebsiella Pneumoniae ◽

Teaching Hospital ◽

Companion Animals ◽

Mlst Analysis ◽

E Coli ◽

Esbl Producers

Extended-spectrum [Formula: see text]-lactamase (ESBL)-producing microorganisms often cause severe infections; they have only limited therapeutic alternatives and have emerged in both human and veterinary medicine. Companion animals have prolonged contact with humans and could serve as a reservoir for ESBL strains. Information regarding ESBL producers in companion animals is rather limited compared to that regarding humans. Therefore, the objective of this study was to screen for ESBL producers in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in urine samples collected from dogs and cats in which urinary tract infection was suspected in a veterinary teaching hospital in Taipei, Taiwan. In addition, bla genes, multilocus sequence typing (MLST) analysis, and antibiotic susceptibility testing were performed on these ESBL producers. From December 2011 to March 2013, disc tests of cefotaxime and ceftazidime, with and without clavulanic acid, were performed on 60 E. coli, 22 [Formula: see text]. pneumoniae, and 18 P. mirabilis samples isolated from urine; 2 E. coli and 5 K. pneumoniae samples with the ESBL phenotype were identified. CTX-M-15 was the most frequently found bla gene. CTX-M-14, SHV-11, SHV-12, and SHV-28 were also identified. MLST analysis did not detect a predominant ST type in either E. coli or [Formula: see text]. pneumoniae; nonetheless, K. pneumoniae ST15 and ST11, which are zoonotic agents of public health concern, were detected in our study. All ESBL-producing bacteria exhibited a multidrug-resistant phenotype. The occurrence of ESBL-producing E. coli and K. pneumoniae in companion animals underscores the importance of ESBL screening and epidemiological study in veterinary hospitals.

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