Development of a donor-specific, automated, and cost-effective cytotoxicity assay for human serum on primary porcine cells

2000 ◽  
Vol 32 (5) ◽  
pp. 867-868 ◽  
Author(s):  
G Laaf ◽  
S Schuster ◽  
U Martin ◽  
G Steinhoff ◽  
A Haverich ◽  
...  
Keyword(s):  
Human Serum ◽  
Cost Effective ◽  
Cytotoxicity Assay

scholarly journals An Exonuclease I-Aided Turn-Off Fluorescent Strategy for Alkaline Phosphatase Assay Based on Terminal Protection and Copper Nanoparticles

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 139
Author(s):  
Yan Wang ◽  
Ying Yan ◽  
Xinfa Liu ◽  
Changbei Ma
Keyword(s):  
Alkaline Phosphatase ◽  
Human Serum ◽  
Clinical Diagnosis ◽  
Copper Nanoparticles ◽  
Cost Effective ◽  
Fast Method ◽  
Exonuclease I ◽  
Alkaline Phosphatase Assay ◽  
Phosphatase Assay ◽  
Phosphatase Alkaline

As an important DNA 3′-phosphatase, alkaline phosphatase can repair damaged DNA caused by replication and recombination. It is essential to measure the level of alkaline phosphatase to indicate some potential diseases, such as cancer, related to alkaline phosphatase. Here, we designed a simple and fast method to detect alkaline phosphatase quantitively. When alkaline phosphatase is present, the resulting poly T-DNA with a 3′-hydroxyl end was cleaved by exonuclease I, prohibiting the formation of fluorescent copper nanoparticles. However, the fluorescent copper nanoparticles can be monitored with the absence of alkaline phosphatase. Hence, we can detect alkaline phosphatase with this turn-off strategy. The proposed method is able to quantify the concentration of alkaline phosphatase with the LOD of 0.0098 U/L. Furthermore, we utilized this method to measure the effects of inhibitor Na3VO4 on alkaline phosphatase. In addition, it was successfully applied to quantify the level of alkaline phosphatase in human serum. The proposed strategy is sensitive, selective, cost effective, and timesaving, having a great potential to detect alkaline phosphatase quantitatively in clinical diagnosis.

scholarly journals A DNA-modified hydrogel for simultaneous purification, concentration and detection of targeted cfDNA in human serum

RSC Advances ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 3407-3415 ◽  
Author(s):  
Xinglu Jiang ◽  
Chenggui Zhao ◽  
Xiaobo Fan ◽  
Wei Xu ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Human Serum ◽  
High Specificity ◽  
Cost Effective ◽  
Polyacrylamide Hydrogel ◽  
Low Concentrations ◽  
Modified Polyacrylamide

A cost-effective device based on DNA-modified polyacrylamide hydrogel was designed to simultaneously catch, purify, concentrate, and detect targeted cfDNA by electrophoresis at low concentrations with high specificity and selectivity.

scholarly journals Development of a Dot-Immunoenzyme Filtration Assay for the Detection of Enterovirus 71 Antigen in Human Serum via Immunoglobulin M

2012 ◽  
Vol 40 (3) ◽  
pp. 1122-1129
Author(s):  
A Zhang ◽  
M Zhang ◽  
H Zhang ◽  
H Li ◽  
Q Liu
Keyword(s):  
Human Serum ◽  
Healthy Adult ◽  
Enterovirus 71 ◽  
Foot And Mouth Disease ◽  
Cost Effective ◽  
Immunoglobulin M ◽  
Healthy Controls ◽  
Structural Protein ◽  
Serum Samples ◽  
Effective Screening

OBJECTIVES: This study evaluated the sensitivity and specificity of a rapid, sensitive dot-immunoenzyme filtration assay to detect enterovirus 71 (EV71) antigen in serum samples from paediatric patients with hand, foot and mouth disease (HFMD), through detection of anti-EV71 immunoglobulin (Ig)M. METHODS: Serum samples from HFMD patients and healthy adult controls were evaluated for the presence of anti-EV71 IgM using a dot-immunoenzyme filtration assay (DIEFA). The results were compared with those obtained using a dot-immunogold filtration assay (DIGFA). The EV71 structural protein VP1 was used as the antigen for both assays. RESULTS: Serum samples from 72 HFMD patients and 54 healthy controls were evaluated. The DIGFA procedure showed a sensitivity of 98.5% and a specificity of 100%, whereas the DIEFA procedure showed a sensitivity of 98.6% and a specificity of 98.0%. There were no significant differences between the assays in either specificity or sensitivity. CONCLUSION: The DIEFA procedure developed in this study has potential as a rapid, simple, sensitive and cost-effective screening technique for detecting EV71 antigen in serum samples from patients with HFMD.

Direct electrochemical analysis in complex samples using ITO electrodes modified with permselective membranes consisting of vertically ordered silica mesochannels and micelles

2015 ◽  
Vol 51 (100) ◽  
pp. 17736-17739 ◽  
Author(s):  
Fei Yan ◽  
Wenjing Zheng ◽  
Lina Yao ◽  
Bin Su
Keyword(s):  
Human Serum ◽  
Electrochemical Detection ◽  
Cost Effective ◽  
Electrochemical Analysis ◽  
Complex Media ◽  
Complex Samples ◽  
Redox Active ◽  
Cost Effective Method ◽  
Pre Treatment ◽  
Organic Analytes

Herein we report a simple and cost-effective method for direct electrochemical detection of redox-active small organic analytes in complex media, such as soil dispersions, human serum and milk, without sample pre-treatment.

scholarly journals In Vitro Quantified Determination of β-Amyloid 42 Peptides, a Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using a Simple, Cost-effective Thin Gold Film Biosensor

2017 ◽  
Author(s):  
Yifan Dai ◽  
Alireza Molazemhosseini ◽  
Chung Chiun Liu
Keyword(s):  
Human Serum ◽  
Tau Protein ◽  
Gold Film ◽  
Cost Effective ◽  
Beta Amyloid ◽  
Sensor Technology ◽  
Counter Electrodes ◽  
Thin Gold Film ◽  
Current Output

A simple in vitro biosensor for the detection of β-amyloid 42 in phosphate-buffer saline (PBS) and undiluted human serum was fabricated and tested based on our platform sensor technology. The bio-recognition mechanism of this biosensor was based on the effect of the interaction between antibody and antigen of β-amyloid 42 to the redox couple probe of K4Fe (CN) 6 and K3Fe (CN) 6. Differential pulse voltammetry (DPV) served as the transduction mechanism measuring the current output derived from the redox coupling reaction. The biosensor was a three-electrode electrochemical system, and the working and counter electrodes were 50 nm thin gold film deposited by sputtering technique. The reference electrode was a thick-film printed Ag/AgCl electrode.   Laser ablation technique was used to define the size and structure of the biosensor. Cost-effective roll-to-roll manufacturing process was employed in the fabrication of the biosensor making it simple and relatively inexpensive. Self-assembled monolayers (SAM) of 3-Mercaptopropionic acid (MPA) was employed to covalently immobilize the thiol group on the gold working electrode. A carbodiimide conjugation approach using N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) and N–hydroxysuccinimide (NHS) was undertaken for cross-linking antibody of β-amyloid 42 to the carboxylic groups on one end of the MPA.  The antibody concentration of β-amyloid 42 used was 18.75µg/mL. The concentration range of β-amyloid 42 in this study was from 0.0675µg/mL to 0.5µg/mL for both PBS and undiluted human serum.    DPV measurements showed excellent response in this antigen concentration range. Interference study of this biosensor was carried out in the presence of Tau protein antigen. Excellent specificity of this β-amyloid 42 biosensor was demonstrated without interference by other species such as T-tau protein. 

scholarly journals Novel Carbon/PEDOT/PSS-Based Screen-Printed Biosensors for Acetylcholine Neurotransmitter and Acetylcholinesterase Detection in Human Serum

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1539 ◽  
Author(s):  
Ashmawy ◽  
Almehizia ◽  
Youssef ◽  
El-Galil E. Amr ◽  
Al-Omar ◽  
...  
Keyword(s):  
Human Serum ◽  
High Selectivity ◽  
Colorimetric Method ◽  
Cost Effective ◽  
Solid Contact ◽  
Sensor Membrane ◽  
Linear Plot ◽  
Materials Used ◽  
The Stability ◽  
Screen Printed

New reliable and robust potentiometric ion-selective electrodes were fabricated using poly(3,4-ethylenedioxythiophene)/poly(styrenesulfonate) (PEDOT/PSS) as the solid contact between the sensing membrane and electrical substrate for an acetylcholine (ACh) bioassay. A film of PEDOT/PSS was deposited on a solid carbon screen-printed platform made from ceramic substrate. The selective materials used in the ion-selective electrode (ISE) sensor membrane were acetylcholinium tetraphenylborate (ACh/TPB/PEDOT/PSS-ISE) (sensor I) and triacetyl-β-cyclodextrin (β-CD/PEDOT/PSS-ISE) (sensor II). The sensors revealed clear enhanced Nernstian response with a cationic slope 56.4 ± 0.6 and 55.3 ± 1.1 mV/decade toward (ACh+) ions over the dynamic linear range 1.0 × 10−6–1 × 10−3 and 2.0 × 10−6–1.0 × 10−3 M at pH 5 with limits of detection 2.0 × 10−7 and 3.2 × 10−7 M for sensors I and II, respectively. The selectivity behavior of both sensors was also tested and the sensors showed a significant high selectivity toward ACh+ over different common organic and inorganic cations. The stability of the potential response for the solid-contact (SC)/ISEs was evaluated using a chronopotentiometric method and compared with that of electrodes prepared without adding the solid-contact material (PEDOT/PSS). Enhanced accuracy, excellent repeatability, good reproducibility, potential stability, and high selectivity and sensitivity were introduced by these cost-effective sensors. The sensors were also used to measure the activity of acetylcholinesterase (AChE). A linear plot between the initial rate of the hydrolysis of ACh+ substrate and enzyme activity held 5.0 × 10−3–5.2 IU∙L−1 of AChE enzyme. Application to acetylcholine determination in human serum was done and the results were compared with the standard colorimetric method.

scholarly journals Facile Preparation of Fe3O4/C Nanocomposite and Its Application for Cost-Effective and Sensitive Detection of Tryptophan

Biomolecules ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 245 ◽  
Author(s):  
Jun Liu ◽  
Shuai Dong ◽  
Quanguo He ◽  
Suchun Yang ◽  
Mei Xie ◽  
...  
Keyword(s):  
Human Serum ◽  
Cost Effective ◽  
X Ray Diffraction ◽  
Modified Glassy Carbon Electrode ◽  
Serum Samples ◽  
One Pot ◽  
Human Serum Samples ◽  
Sensitive Determination ◽  
The Cost

In this study, we reported facile synthesis of Fe3O4/C composite and its application for the cost-effective and sensitive determination of tryptophan (Trp) in human serum samples. Fe3O4/C composites were prepared by a simple one-pot hydrothermal method followed by a mild calcination procedure, using FeCl3∙6H2O as Fe3O4 precursor, and glucose as reducing agent and carbon source simultaneously. The Fe3O4/C composite modified glassy carbon electrode (Fe3O4/C/GCE) was prepared by drop-casting method. The microstructure and morphology of Fe3O4/C composite was characterized by powder X-ray diffraction (XRD) and scanning electron microscopy (SEM), respectively. Due to large specific surface area and synergistic effect from Fe3O4 nanoparticles and carbon coating, Fe3O4/C composite showed excellent electrocatalytic activity toward the oxidation of Trp. As a result, the proposed Fe3O4/C/GCE displayed superior analytical performances toward Trp determination, with two wide detection ranges (1.0–80 μM and 80–800 μM) and a low detection limit (0.26 μM, S/N = 3). Moreover, successful detection of Trp in human serum samples further validate the practicability of the proposed sensor.

scholarly journals A Fluorescence Inner-Filter Effect Based Sensing Platform for Turn-On Detection of Glutathione in Human Serum

Sensors ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 228 ◽  
Author(s):  
Shurong Tang ◽  
Xiuhua You ◽  
Quanhui Fang ◽  
Xin Li ◽  
Guangwen Li ◽  
...  
Keyword(s):  
Human Serum ◽  
Limit Of Detection ◽  
Cost Effective ◽  
Inner Filter Effect ◽  
Disease Diagnosis ◽  
Fluorescent Properties ◽  
Serum Samples ◽  
Turn On ◽  
Filter Effect ◽  
Sensing Platform

A novel turn-on fluorescence assay was developed for the rapid detection of glutathione (GSH) based on the inner-filter effect (IFE) and redox reaction. Molybdenum disulfide quantum dots (MoS2 QDs), which have stable fluorescent properties, were synthesized with hydrothermal method. Manganese dioxide nanosheets (MnO2 NSs) were prepared by exfoliating the bulk δ-MnO2 material in bovine serum albumin (BSA) aqueous solution. The morphology structures of the prepared nanoparticles were characterized by transmission electron microscope (TEM). Studies have shown that the fluorescence of MoS2 QDs could be quenched in the presence of MnO2 NSs as a result of the IFE, and is recovered after the addition of GSH to dissolve the MnO2 NSs. The fluorescence intensity showed a good linear relationship with the GSH concentration in the range 20–2500 μM, the limit of detection was 1.0 μM. The detection method was applied to the analysis of GSH in human serum samples. This simple, rapid, and cost-effective method has great potential in analyzing GSH and in disease diagnosis.

scholarly journals A Protocol for Measurement of Noncoding RNA in Human Serum

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Caroline J. Taylor ◽  
Sarang N. Satoor ◽  
Amaresh K. Ranjan ◽  
Maria V. Pereira e Cotta ◽  
Mugdha V. Joglekar
Keyword(s):  
Human Serum ◽  
Noncoding Rna ◽  
Noncoding Rnas ◽  
Cost Effective ◽  
Pcr Analysis ◽  
Messenger Rnas ◽  
Initial Report ◽  
Small Noncoding Rnas ◽  
Liquid Samples ◽  
Cost Effective Method

MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Currently, there are many methods of isolating total RNA from liquid samples. Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels.

scholarly journals Brine shrimp lethality assay

2017 ◽  
Vol 12 (2) ◽  
pp. 5 ◽  
Author(s):  
Quazi Sahely Sarah ◽  
Fatema Chowdhury Anny ◽  
Mir Misbahuddin
Keyword(s):  
Plant Extract ◽  
Brine Shrimp ◽  
Video Clip ◽  
Cost Effective ◽  
Cytotoxicity Assay ◽  
Test Material ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay ◽  
Full Screen

<p>Brine shrimp lethality assay is an important tool for the preliminary cytotoxicity assay of plant extract and others based on the ability to kill a laboratory cultured larvae (nauplii). The nauplii were exposed to different concentrations of plant extract for 24 hours. The number of motile nauplii was calculated for the effectiveness of the extract. It is a simple, cost effective and requires small amount of test material.</p><p><strong>Video Clip of Methodology</strong>:</p><p>Brine Shrimp Lethality Assay: 4 min 9 sec   <a href="https://www.youtube.com/v/QJY7SQogD0g">Full Screen</a>   <a href="https://www.youtube.com/watch?v=QJY7SQogD0g">Alternate</a>   </p>

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