Characterization of coxsackie B virus RNA in myocardium from patients with dilated cardiomyopathy by nucleotide sequencing of reverse transcription-nested polymerase chain reaction products

1998 ◽  
Vol 29 (6) ◽  
pp. 578-584 ◽  
Author(s):  
Leonard C. Archard ◽  
Mahboob A. Khan ◽  
Bambos A. Soteriou ◽  
Hongyi Zhang ◽  
Howard J.F. Why ◽  
...  
Keyword(s):  
Nucleotide Sequencing ◽  
Reaction Products ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Virus Rna ◽  
B Virus ◽  
Coxsackie B Virus ◽  
Polymerase Chain ◽  
Coxsackie B

Detection of Coxsackie B Virus RNA sequences in whole blood samples from adult patients at the onset of type I diabetes mellitus

1997 ◽  
Vol 52 (2) ◽  
pp. 121-127 ◽  
Author(s):  
Laurent Andréoletti ◽  
Didier Hober ◽  
Christine Hober-Vandenberghe ◽  
Sandrine Belaich ◽  
Marie-Christine Vantyghem ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type I Diabetes ◽  
Type I ◽  
Rna Sequences ◽  
Blood Samples ◽  
Virus Rna ◽  
B Virus ◽  
Coxsackie B Virus ◽  
Whole Blood Samples ◽  
Coxsackie B

scholarly journals Characterization of a Phytoplasma Associated with Frogskin Disease in Cassava

Plant Disease ◽  
2009 ◽  
Vol 93 (11) ◽  
pp. 1139-1145 ◽  
Author(s):  
Elizabeth Alvarez ◽  
Juan F. Mejía ◽  
Germán A. Llano ◽  
John B. Loke ◽  
Alberto Calari ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Manihot Esculenta ◽  
Fragment Length Polymorphism ◽  
Rrna Gene ◽  
South American ◽  
Sequence Analyses ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

Cassava frogskin disease (CFSD) is an economically important root disease of cassava (Manihot esculenta) in Colombia and other South American countries, including Brazil, Venezuela, Peru, Costa Rica, and Panama. The roots of severely affected plants are thin, making them unsuitable for consumption. In Colombia, phytoplasma infections were confirmed in 35 of 39 genotypes exhibiting mild or severe CFSD symptoms either by direct or nested polymerase chain reaction (PCR) assays employing ribosomal (r)RNA operon primer pairs. The CFSD-associated phytoplasmas were identified as group 16SrIII strains by restriction fragment length polymorphism (RFLP) and sequence analyses of amplified rDNA products, and results were corroborated by PCRs employing group 16SrIII-specific rRNA gene or ribosomal protein (rp) gene primers. Collectively, RFLP analyses indicated that CFSD strains differed from all phytoplasmas described previously in group 16SrIII and, on this basis, the strains were tentatively assigned to new ribosomal and ribosomal protein subgroups 16SrIII-L and rpIII-H, respectively. This is the first molecular identification of a phytoplasma associated with CFSD in cassava in Colombia.

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