Addition of insulin to bovine antral follicles in vitro induces ultrastructural and protein profile changes characteristic of mature oocytes

1999 ◽  
Vol 51 (1) ◽  
pp. 372
Author(s):  
A.A Fouladi-Nashta ◽  
K.H.S Campbell
Keyword(s):  
Protein Profile ◽  
Antral Follicles ◽  
Profile Changes

Should fertile women quit drinking alcohol to produce better quality oocytes?

Zygote ◽  
2020 ◽  
pp. 1-3
Author(s):  
Burcu Ozbakir ◽  
Pinar Tulay
Keyword(s):  
Alcohol Consumption ◽  
Young Women ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Control Group ◽  
Social Drinkers ◽  
Antral Follicles ◽  
Healthy Women ◽  
Significant Difference

Summary Alcohol consumption has long been shown to affect both fetal health and pregnancy. In this study, antral follicle count, maturation level of oocytes including morphological assessment and number of metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) stage oocytes obtained from young women (age < 30 years old) with or without alcohol consumption were investigated. In total, 20 healthy women who were social drinkers and 36 healthy women who do not consume alcohol were involved in this study. Women in both study and control groups were undergoing controlled ovarian stimulation. The antral follicle count and the number and quality of the oocytes retrieved were evaluated and recorded. In total, 635 antral follicles, 1098 follicles and 1014 oocytes with 820 MII, 72 MI and 78 GV stage oocytes were collected from the social drinkers. In the control group, 628 antral follicles, 1136 follicles and 1085 oocytes with 838 MII, 93 MI and 102 GV stage oocytes were evaluated. The results of this study showed that the antral follicle count was very similar in both groups. The number of oocytes and MII stage oocytes was slightly higher in the control group, although it was not a significant difference. This study showed that although the consumption of alcohol may have adverse effects post-implantation, it may not have a solid effect during oogenesis in young women. The results of this study are especially important in clinical settings as some women who are social drinkers undergo in vitro fertilization treatments.

scholarly journals Salt-induced root protein profile changes in seedlings of maize inbred lines with differing salt tolerances

2014 ◽  
Vol 74 (4) ◽  
pp. 468-476 ◽  
Author(s):  
Yujing Cheng ◽  
Guoqing Chen ◽  
Derong Hao ◽  
Huhua Lu ◽  
Mingliang Shi ◽  
...  
Keyword(s):  
Protein Profile ◽  
Inbred Lines ◽  
Maize Inbred Lines ◽  
Profile Changes

scholarly journals Copper-stress induced alterations in protein profile and antioxidant enzymes activities in the in vitro grown Withania somnifera L.

2013 ◽  
Vol 19 (3) ◽  
pp. 353-361 ◽  
Author(s):  
Jyoti R. Rout ◽  
Shidharth S. Ram ◽  
Ritarani Das ◽  
Anindita Chakraborty ◽  
Mathummal Sudarshan ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Withania Somnifera ◽  
Protein Profile ◽  
Copper Stress ◽  
Enzymes Activities ◽  
Antioxidant Enzymes Activities

scholarly journals Detection of Nuclear Protein Profile Changes by Human Metapneumovirus M2-2 Protein Using Quantitative Differential Proteomics

Vaccines ◽  
2017 ◽  
Vol 5 (4) ◽  
pp. 45 ◽  
Author(s):  
Yuping Ren ◽  
Eunjin Choi ◽  
Ke Zhang ◽  
Yu Chen ◽  
Sha Ye ◽  
...  
Keyword(s):  
Nuclear Protein ◽  
Protein Profile ◽  
Human Metapneumovirus ◽  
Differential Proteomics ◽  
Profile Changes

scholarly journals Involvement of hyaluronan synthesis in ovarian follicle growth in rats

Reproduction ◽  
2014 ◽  
Vol 147 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Noriyuki Takahashi ◽  
Wataru Tarumi ◽  
Bunpei Ishizuka
Keyword(s):  
Ovarian Follicle ◽  
Primary Site ◽  
Corpora Lutea ◽  
Follicular Atresia ◽  
Follicle Growth ◽  
Adult Rats ◽  
Antral Follicles ◽  
Cell Layers ◽  
Ha Synthase

Most of the previous studies on ovarian hyaluronan (HA) have focused on mature antral follicles or corpora lutea, but scarcely on small preantral follicles. Moreover, the origin of follicular HA is unknown. To clarify the localization of HA and its synthases in small growing follicles, involvement of HA in follicle growth, and gonadotropin regulation of HA synthase (Has) gene expression, in this study, perinatal, immature, and adult ovaries of Wistar-Imamichi rats were examined histologically and biochemically and byin vitrofollicle culture. HA was detected in the extracellular matrix of granulosa and theca cell layers of primary follicles and more advanced follicles. Ovarian HA accumulation ontogenetically started in the sex cords of perinatal rats, and its primary site shifted to the intrafollicular region of primary follicles within 5 days of birth. TheHas1–3mRNAs were expressed in the ovaries of perinatal, prepubertal, and adult rats, and the expression levels ofHas1andHas2genes were modulated during the estrous cycle in adult rats and following administration of exogenous gonadotropins in immature acyclic rats. TheHas1andHas2mRNAs were predominantly localized in the theca and granulosa cell layers of growing follicles respectively. Treatments with chemicals known to reduce ovarian HA synthesis induced follicular atresia. More directly, the addition ofStreptomyceshyaluronidase, which specifically degrades HA, induced the arrest of follicle growth in anin vitroculture system. These results indicate that gonadotropin-regulated HA synthesis is involved in normal follicle growth.

scholarly journals Evaluation of amniotic hydrogel’s impact on the development of murine pre-antral follicles in mouse model

2019 ◽  
Vol 2 (4) ◽  
pp. 32-39
Author(s):  
Van Ngoc Le Trinh ◽  
Hang Thi Kim Tran ◽  
Thu Thuy Anh Vo ◽  
Tuyet Thi Vi Le ◽  
Ha Le Bao Tran
Keyword(s):  
In Vitro Maturation ◽  
High Demand ◽  
Antral Follicles ◽  
Secondary Follicle ◽  
The Moment ◽  
Main Component ◽  
Supporting Frame ◽  
Mature Process

Global average infertility rate is about 6– 12%, and in Vietnam at around 7.7%. As a result, there is a high demand for treatment, especially for female infertility. In vitro maturation (IVM) was evaluated and proven to be the most popular and promising at the moment. In long-term cultivation, the follicle was observed to extend, therefore, the usage of a supporting frame is quite necessary to maintain follicle’s natural sphere structure as well as completing the mature process. Amniotic membrane is an avascular membrane, composed of collagen, fibronectin, nidogen, proteoglycan, containing a big number of growth – factors with antimicrobial, anti-inflammatory, low immunogenicity and viscoelasticity properties. Amniotic hydrogel owns structure formed with thin fibers to help preserve the main component as collagen, which can turn to gel form at 37 degree Celsius. With those properties, amniotic hydrogel showed high potential as a scaffold for the follicle. When amniotic hydrogel is used as a scaffold for cultivating of secondary follicle (100 – 130 µm), the size of oocyte and follicle increased after 12 days of culturing, along with the formation of antrum. The results demonstrated the possibility to use amniotic hydrogel as a scaffold for the development of the secondary follicle.

scholarly journals Embryo transfer following in vitro maturation and cryopreservation of oocytes recovered from antral follicles during conservative surgery for ovarian cancer

2012 ◽  
Vol 29 (8) ◽  
pp. 779-781 ◽  
Author(s):  
Rubens Fadini ◽  
Mariabeatrice Dal Canto ◽  
Mario Mignini Renzini ◽  
Rodolfo Milani ◽  
Robert Fruscio ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Embryo Transfer ◽  
In Vitro Maturation ◽  
Conservative Surgery ◽  
Antral Follicles
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