1010 poster Increased radiosensitizing effect of bleomycin or cisplatin on LPB sarcoma tumor cells and tumors with localy drug delivery method, electroporation

Computational tools have been used to study the photophysical and photochemical features of photosensitizers in photodynamic therapy (PDT) –a minimally invasive, less aggressive alternative for cancer treatment. PDT is mainly based by the activation of molecular oxygen through the action of a photoexcited sensitizer (photosensitizer). Temoporfin, widely known as mTHPC, is a second-generation photosensitizer, which produces the cytotoxic singlet oxygen when irradiated with visible light and hence destroys tumor cells. However, the bioavailability of the mostly hydrophobic photosensitizer, and hence its incorporation into the cells, is fundamental to achieve the desired effect on malignant tissues by PDT. In this study, we focus on the optical properties of the temoporfin chromophore in different environments –in <i>vacuo</i>, in solution, encapsulated in drug delivery agents, namely cyclodextrin, and interacting with a lipid bilayer.

Download Full-text

Transferrin-Conjugated Nanocarriers as Active-Targeted Drug Delivery Platforms for Cancer Therapy

Current Pharmaceutical Design ◽

10.2174/1381612822666161026162347 ◽

2017 ◽

Vol 23 (3) ◽

pp. 454-466 ◽

Cited By ~ 16

Author(s):

Daniele R. Nogueira-Librelotto ◽

Cristiane F. Codevilla ◽

Ammad Farooqi ◽

Clarice M. B. Rolim

Keyword(s):

Drug Delivery ◽

Cancer Therapy ◽

Tumor Cells ◽

Therapeutic Benefit ◽

Active Targeting ◽

Future Research ◽

Passive Targeting ◽

The Right ◽

Review Current ◽

Target Effects

A lot of effort has been devoted to achieving active targeting for cancer therapy in order to reach the right cells. Hence, increasingly it is being realized that active-targeted nanocarriers notably reduce off-target effects, mainly because of targeted localization in tumors and active cellular uptake. In this context, by taking advantage of the overexpression of transferrin receptors on the surface of tumor cells, transferrin-conjugated nanodevices have been designed, in hope that the biomarker grafting would help to maximize the therapeutic benefit and to minimize the side effects. Notably, active targeting nanoparticles have shown improved therapeutic performances in different tumor models as compared to their passive targeting counterparts. In this review, current development of nano-based devices conjugated with transferrin for active tumor-targeting drug delivery are highlighted and discussed. The main objective of this review is to provide a summary of the vast types of nanomaterials that have been used to deliver different chemotherapeutics into tumor cells, and to ultimately evaluate the progression on the strategies for cancer therapy in view of the future research.

Download Full-text

Peptide-conjugated PEGylated PAMAM as a highly affinitive nanocarrier towards HER2-overexpressing cancer cells

RSC Advances ◽

10.1039/c6ra19552k ◽

2016 ◽

Vol 6 (109) ◽

pp. 107337-107343 ◽

Cited By ~ 10

Author(s):

Iman Rostami ◽

ZiJian Zhao ◽

ZiHua Wang ◽

WeiKai Zhang ◽

Yeteng Zhong ◽

...

Keyword(s):

Drug Delivery ◽

Cancer Cells ◽

Tumor Cells ◽

Targeting Peptide

Efficient drug delivery to the tumor cells was carried out with HER2 targeting peptide-conjugated PEGlyted PAMAM.

Download Full-text

P10.02 Combined methods of a micropump system and a chronic cranial window allows tumor observation with multi photon laser scanning microscopy under continuous treatment

Neuro-Oncology ◽

10.1093/neuonc/noab180.095 ◽

2021 ◽

Vol 23 (Supplement_2) ◽

pp. ii28-ii28

Author(s):

S Weil ◽

E Jung ◽

D Domínguez Azorín ◽

J Higgins ◽

J Reckless ◽

...

Keyword(s):

Drug Delivery ◽

Tumor Cells ◽

Blood Brain Barrier ◽

Laser Scanning ◽

Laser Scanning Microscopy ◽

New Drugs ◽

Brain Barrier ◽

Cranial Window ◽

Blood Brain ◽

The Brain

Abstract BACKGROUND Glioblastomas are notoriously therapy resistant tumors. As opposed to other tumor entities, no major advances in therapeutic success have been made in the past decades. This has been calling for a deeper biological understanding of the tumor, its growth and resistance patterns. We have been using a xenograft glioma model, where human glioblastoma cells are implanted under chronic cranial windows and studied longitudinally over many weeks and months using multi photon laser scanning microscopy (MPLSM). To test the effect of (new) drugs, a stable and direct delivery system avoiding the blood-brain-barrier has come into our interest. MATERIAL AND METHODS We implanted cranial windows and fluorescently labeled human glioblastoma stem-like cells into NMRI nude mice to follow up on the tumor development in our MPLSM model. After tumor establishment, an Alzet® micropump was implanted to directly deliver agents via a catheter system continuously over 28 days directly under the cranial window onto the brain surface. Using the MPLSM technique, the continuous delivery and infusion of drugs onto the brain and into the tumor was measured over many weeks in detail using MPLSM. RESULTS The establishment of the combined methods allowed reliable concurrent drug delivery over 28 days bypassing the blood-brain-barrier. Individual regions and tumor cells could be measured and followed up before, and after the beginning of the treatment, as well as after the end of the pump activity. Fluorescently labelled drugs were detectable in the MPLSM and its distribution into the brain parenchyma could be quantified. After the end of the micropump activity, further MPLSM measurements offer the possibility to observe long term effects of the applied drug on the tumor. CONCLUSION The combination of tumor observation in the MPSLM and concurrent continuous drug delivery is a feasible and reliable method for the investigation of (novel) anti-tumor agents, especially drugs that are not blood-brain-barrier penetrant. Morphological or even functional changes of individual tumor cells can be measured under and after treatment. These techniques can be used to test new drugs targeting the tumor, its tumor microtubes and tumor cells networks, and measure the effects longitudinally.

Download Full-text

Curcumin loaded ultrasound contrast agents for drug delivery to tumor cells

10.17918/etd-3374 ◽

2021 ◽

Author(s):

Archana Sidalaghatta Nagaraja

Keyword(s):

Drug Delivery ◽

Contrast Agents ◽

Tumor Cells ◽

Ultrasound Contrast Agents ◽

Ultrasound Contrast

Download Full-text

A comprehensive review on buccal patches

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2020.13.1.0308 ◽

2020 ◽

Vol 13 (1) ◽

pp. 130-135

Author(s):

Seema ◽

Kapil kumar ◽

Deepak Teotia

Keyword(s):

Drug Delivery ◽

Oral Cavity ◽

Systemic Circulation ◽

Drug Formulation ◽

Delivery Method ◽

Modern Approach ◽

Comprehensive Review ◽

First Pass Metabolism ◽

First Pass ◽

Pass Metabolism

Buccal Patches are the type of drug formulation that has normally a different course of administration through the buccal mucosa for drug delivery. The product is placed between upper gingiva (gums) and cheek to treat local and systemic conditions. Buccal patch have good accessibility to the membranes that line the oral cavity. These patches tend to help drug enter directly into the systemic circulation escaping hepatic first pass metabolism. This type of drug delivery method is considered useful for elevating the bioavailability of drugs. This review is a thorough study to apprehend the procedures involved in assessment of buccal patches and the modern approach towards this type of drug delivery. This article intends to analyze the overall profile of Buccal Patches and scope of future advances.

Download Full-text

Exosomes containing miRNAs targeting HER2 synthesis and engineered to adhere to HER2 on tumor cells surface exhibit enhanced antitumor activity

10.21203/rs.3.rs-39552/v2 ◽

2020 ◽

Author(s):

Lei Wang ◽

Xusha Zhou ◽

Weixuan Zou ◽

Yinglin Wu ◽

Jing Zhao ◽

...

Keyword(s):

Drug Delivery ◽

Epidermal Growth Factor Receptor ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Cancer Cells ◽

Tumor Cells ◽

Growth Factor Receptor ◽

Wide Range ◽

Epidermal Growth

Abstract Background: Exosomes are small, cellular membrane-derived vesicles with a diameter of 50-150 nm. Exosomes are considered ideal drug delivery systems with a wide range of applications in various diseases, including cancer. However, nonspecific delivery of therapeutic agents by exosomes in vivo remains challenging. H uman epidermal growth factor receptor 2 (HER2) is an epidermal growth factor receptor tyrosine kinase, and its overexpression is usually associated with cell survival and tumor progression in various cancers. In this study, we aim to develop novel exosomes with dual HER2-targeting ability as a nanoparticle delivery vehicle to enhance antitumor efficacy in vivo . Results: Here, we report the generation of two kinds of exosomes carrying miRNAs designed to block HER2 synthesis and consequently kill tumor cells. 293-miR-HER2 exosomes package and deliver designed miRNAs to cells to block HER2 synthesis. These exosomes kill cancer cells dependent on HER2 for survival but do not affect cells that lack HER2 or that are engineered to express HER2 but are not dependent on it for survival. In contrast, 293-miR-XS-HER2 exosomes carry an additional peptide, which enables them to adhere to HER2 on the surface of cancer cells. Consequently, these exosomes preferentially enter and kill cells with surface expression of HER2. 293-miR-XS-HER2 exosomes are significantly more effective than the 293-miR-HER2 exosomes in shrinking HER2-positive tumors implanted in mice. Conclusions: Collectively, as novel antitumor drug delivery vehicles, HER2 dual-targeting exosomes exhibit increased target-specific delivery efficiency and can be further utilized to develop new nanoparticle-based targeted therapies.

Download Full-text

Transglutaminase mediated PEGylation of nanobodies for targeted nano-drug delivery

Journal of Materials Chemistry B ◽

10.1039/c7tb03132g ◽

2018 ◽

Vol 6 (7) ◽

pp. 1011-1017 ◽

Cited By ~ 9

Author(s):

Tiantian Wu ◽

Hai Huang ◽

Yaping Sheng ◽

Hongdong Shi ◽

Yuanzeng Min ◽

...

Keyword(s):

Drug Delivery ◽

Tumor Cells ◽

Drug Delivery System ◽

Delivery System ◽

Nano Drug Delivery

The PEGylation of anti-EGFR nanobodies was achieved via a transglutaminase catalyzed reaction through a Q-tag. The nanobody tethered drug delivery system exhibits superior specificity to EGFR positive tumor cells.

Download Full-text